公开(公告)号：WO2015001173A1

公开(公告)日：2015-01-08

申请号：PCT/FI2014/000014

申请日：2014-07-01

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias ,  SUHONEN, Anssi

IPC: C12M1/34 ,  C12M1/36 ,  C12M1/12 ,  C12M1/04 ,  A61L2/07 ,  A61L2/10 ,  A61L2/20

CPC classification number: C12M37/00 ,  A61L2/07 ,  A61L2/10 ,  A61L2/20 ,  C12M23/28 ,  C12M33/04 ,  C12M37/02 ,  C12M37/06 ,  C12M41/14

Abstract: A microbiological cultivation method, and a device for its use is equipped with field-capable system for producing optimal culture conditions for aerobic, microaerobic and anaerobic microbes. They are further equipped with possibilities for independent clean up and sterilization, as well as for gas supply, generation and regulation. This device and method can be used for clinical, environmental and industrial microbial monitoring tasks.

Abstract translation: 微生物培养方法及其使用装置配备有田间能力系统，用于产生需氧，微需氧和厌氧微生物的最佳培养条件。 他们进一步配备独立清理和灭菌以及供气，生成和调节的可能性。 该装置和方法可用于临床，环境和工业微生物监测任务。

公开(公告)号：WO2016001479A1

公开(公告)日：2016-01-07

申请号：PCT/FI2015/000031

申请日：2015-07-01

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: C12P3/00 ,  C12M1/04 ,  C12M1/107 ,  C12M1/34 ,  B09B3/00 ,  C10L5/04 ,  C22B3/18

CPC classification number: C12P3/00 ,  C12M43/04

Abstract: The present invention describes a fermentation process aiming to clean lignite (Figure 1 ), with the purpose of improving the usability of lignite in energy production from the viewpoint of environmental protection, process technology, and overall economic advantage. Characteristic of the equipment used is that its operation is adjusted and optimized by measuring and adjusting the composition and flow rates of gas flows.

Abstract translation: 本发明描述了一种旨在清除褐煤的发酵方法（图1），其目的是从环境保护，工艺技术和总体经济优势的角度提高褐煤在能源生产中的可用性。 使用的设备的特点是通过测量和调整气体流量的组成和流量来调整和优化其操作。

公开(公告)号：WO2015052369A1

公开(公告)日：2015-04-16

申请号：PCT/FI2014/000026

申请日：2014-10-08

Applicant: HAKALEHTO, Eino, Elias

Inventor： HAKALEHTO, Eino, Elias

IPC: C12P7/18 ,  C12P7/56 ,  C12P7/52 ,  C12P19/24

CPC classification number: C12P7/52 ,  C12M29/00 ,  C12P7/04 ,  C12P7/18 ,  C12P7/40

Abstract: Slaughter waste normally contains considerable amounts of so-called stomach manure. Its biotechnical treatment rapidly decreases the waste problem. With the help of the method and device according to this invention stomach manure is first used as a biocatalyst in a process that produces mannitol. After that the lactic acid that at the same time farms into the slaughter waste Is converted with the help of microbes into propionic acid or other useful compounds.

Abstract translation: 屠宰废物通常含有大量所谓的胃肥。 其生物技术处理迅速减少了废物的问题。 借助根据本发明的方法和装置，在产生甘露醇的过程中首先将胃肥用作生物催化剂。 之后，在微生物的帮助下将同时进入屠宰废物的乳酸转化成丙酸或其他有用的化合物。

公开(公告)号：WO2022038306A1

公开(公告)日：2022-02-24

申请号：PCT/FI2021/000008

申请日：2021-08-17

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: C12P7/18 ,  C12P7/56 ,  C12M1/00 ,  C12P39/00 ,  C12P7/40

Abstract: In the combined process for several biorefinery products obtained from a UMC (Undefined Mixed Culture) type of reaction it is possible to obtain biochemicals, energy gases, soil improvement etc. from a MPBU (Multipurpose Biorefinery Unit). The economically beneficial as well as environmentally sustainable results of the arrangement are demonstrated by the integrated process using two reactor systems with zero fiber for the production of lactate (in both the reactors pools 1 and 2). Additionally, mannitol can be produced in one of the reactor pools (number 2). It is possible to a. combine the processes taking into account their biochemical characteristics, b. produce gaseous substances for energy and industrial use, c. obtain organic fertilizers which can be microbiologically upgraded d. improve the adjustability for optimization of the various partial reactivities. The chemical production occurs in two pools which advantageously are inoculated simultaneously.

公开(公告)号：WO2017051058A1

公开(公告)日：2017-03-30

申请号：PCT/FI2015/000042

申请日：2015-11-13

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: C05F11/08 ,  C05C11/00 ,  C12P3/00 ,  C05F1/00 ,  C05F3/00 ,  C05F7/00 ,  C05F9/00 ,  C05F17/00 ,  C12M1/00 ,  C12N1/00

CPC classification number: C05C11/00 ,  C05F1/00 ,  C05F11/08 ,  C12N1/20 ,  Y02A40/201 ,  Y02P20/145 ,  Y02W30/43 ,  Y02W30/47

Abstract: The effect of organic and other fertilizers to plant nutrition can be improved with the help of microbes. With a method and device according to this invention, especially the usefulness of nitrogen to plants is increased when microbes that affect its circulation in an advantageous way, in respect to plant nutrition, are added either as partial components of a fertilizer or as such, for example in irrigation water. The added microbes may be used as pure cultures, microbe communities or mixed cultures and they advantageously have an ability for the binding of microbiological nitrogen from air. With the help of Clostridium pasteurianum bacteria both the binding of nitrogen and the biochemical processes in soil that correspond to nitrification, can be accomplished. This enables the improvement of plant nutrition, not only by the interaction of microbe communities, but also with the help of the different enzymatic reactions of a single microbe strain and as their sum effect. In experiments using this procedure a 100% crop increase has been achieved with several different cultivated plants.

Abstract translation: 借助微生物，可以提高有机肥料和其他肥料对植物营养的影响。 使用根据本发明的方法和装置，特别是当植物营养中以有利的方式影响其循环的微生物作为肥料的部分成分添加时，特别是对植物的氮的有效性增加， 例如灌溉水。 添加的微生物可以用作纯培养物，微生物群落或混合培养物，并且它们有利地具有从空气中结合微生物氮的能力。 在巴斯德氏梭菌的帮助下，可以实现与硝化相对应的氮和土壤中生化过程的结合。 这使得植物营养的改善不仅可以通过微生物群落的相互作用，而且还可以借助于单一微生物菌株的不同酶反应和其总效应。 在使用该方法的实验中，已经用几种不同的栽培植物实现了100％的作物增加。

公开(公告)号：WO2012104468A1

公开(公告)日：2012-08-09

申请号：PCT/FI2010/000078

申请日：2010-12-28

Applicant: HAKALEHTO, Eino, Elias

Inventor： HAKALEHTO, Eino, Elias

IPC: C12Q1/04 ,  C12M1/34 ,  C12M1/107 ,  G01N35/02

CPC classification number: C12Q1/04 ,  G01N15/06 ,  G01N15/1459 ,  G01N21/00 ,  G01N35/00 ,  G01N35/02 ,  G01N35/1095 ,  G01N2015/1493

Abstract: By using the method and equipment according to this invention it is possible to analyse rapidly a large number of microbiological culture samples, or alternatively of liquid phase samples, based on the gases or gaseous compounds released by them. This invention exploits generally a sample line, along which the samples move, and it can be used for the microbial control tasks in hospitals, industry, hygiene and environmental fields. In this system gas is led into the culture vessels during the growth of the microbe, and gases released by the culture or liquids derived from them are collected into the chambers, capsules or equivalents.

Abstract translation: 通过使用根据本发明的方法和设备，可以基于由它们释放的气体或气体化合物快速分析大量的微生物培养样品或者液相样品。 本发明一般利用样品移动的样品线，可用于医院，工业，卫生和环境领域的微生物控制任务。 在该系统中，在微生物生长过程中，气体被引入培养容器中，由培养物释放的气体或从其产生的液体被收集到腔室，胶囊或等同物中。

公开(公告)号：WO2011110731A3

公开(公告)日：2011-10-27

申请号：PCT/FI2011000016

申请日：2011-03-09

Applicant: HAKALEHTO EINO ELIAS

Inventor： HAKALEHTO EINO ELIAS

IPC: C12M1/00 ,  C12M1/04 ,  C12M1/36 ,  C12N1/00

CPC classification number: C12P7/18 ,  C12M21/12 ,  C12M23/02 ,  C12P7/16 ,  C12P39/00 ,  Y02E50/10 ,  Y02E50/343

Abstract: The method and apparatus according to the present invention concentrate the formation of the biotechnical product to active site in the reactor, where the production is accelerated to extreme speeds by altering the conditions. To achieve this, anabolic, catabolic or overflow metabolic reactions can be utilised. The aim is to implement production so that carbon dioxide emissions in particular are minimised The products are e.g. 2,3-butanediol, butanol, ethanol, acetone, organic acids, methane or hydrogen gas and other fuels or compounds necessary for chemical or material production.

Abstract translation: 根据本发明的方法和设备将生物技术产品的形成集中到反应器中的活性部位，其中通过改变条件将生产加速到极限速度。 为了达到此目的，可以利用合成代谢，分解代谢或溢流代谢反应。 其目的是实施生产，使二氧化碳排放量特别降到最低。 2,3-丁二醇，丁醇，乙醇，丙酮，有机酸，甲烷或氢气以及化学或材料生产所需的其他燃料或化合物。

公开(公告)号：WO2009125049A1

公开(公告)日：2009-10-15

申请号：PCT/FI2009/000047

申请日：2009-04-07

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: C12M1/00 ,  A01C3/02 ,  C05F3/00 ,  C05F3/06 ,  C05F9/00 ,  C05F9/02 ,  C05F9/04 ,  C05F17/00 ,  C05F17/02

CPC classification number: C12M23/20 ,  C05F17/0018 ,  Y02P20/145 ,  Y02W30/43

Abstract: The use of the equipment and method corresponding with this invention makes it possible to implement biotechnological production from biomass-based raw materials cost-effectively at, or in the vicinity of, raw material sources. Only some sections of the reactor used, such as supporting structures and the component needed for arranging lead-tliroughs, are implemented with a hard material, such as steel. Additionally, plastic, paper, or textile or another material of this type is used to construct the reactor frame.

Abstract translation: 使用与本发明相对应的设备和方法使得可以在原材料来源或附近成本有效地实现基于生物质的原材料的生物技术生产。 所使用的反应堆的一些部分，例如支撑结构和排列导线所需的部件，是用诸如钢的硬质材料来实现的。 此外，塑料，纸或纺织品或这种类型的其他材料用于构建反应器框架。

公开(公告)号：WO2012104475A1

公开(公告)日：2012-08-09

申请号：PCT/FI2012/000004

申请日：2012-02-03

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: C12Q1/04 ,  C12Q1/24 ,  C12M1/04

CPC classification number: C12Q1/04

Abstract: With a method and equipment according to this invention the best possible growth start of hygiene indicators and other bacteria and other microbes in water samples is ensured in microbe cultivations for example on Colilert™ media using both static cultivations and bubbled cultures such as in a PMEU (Portable Microbe Enrichment Unit) type cultivation. The objective is the best possible microbiological detection value of a water sample or process sample regardless of whether the said cultivation is implemented without bubbling or other mixing or with it. The equipment and method according to the invention applies to and can be connected with automatic or manual sampling.

Abstract translation: 使用根据本发明的方法和设备，在微生物培养中确保水样中的卫生指示剂和其它细菌和其它微生物的最佳生长开始，例如在ColilertTM培养基上使用静态栽培和鼓泡培养物，例如在PMEU（Portable 微生物浓缩单位）种植。 目标是水样或过程样品的最佳可能的微生物检测值，无论所述培养是否在不起泡或其它混合的情况下实施。 根据本发明的设备和方法适用于并且可以与自动或手动取样连接。

公开(公告)号：WO2009130367A1

公开(公告)日：2009-10-29

申请号：PCT/FI2009/000053

申请日：2009-04-24

Applicant: HAKALEHTO, Eino Elias

Inventor： HAKALEHTO, Eino Elias

IPC: A23L1/30 ,  A61K35/74 ,  C12Q1/00

CPC classification number: A61K35/74 ,  A23L33/135

Abstract: The method described in this invention allows a new way of regulating the pH conditions and microbial balance of the small intestine and the entire intestinal channel through facultative anaerobic bacteria, making it possible for this regulation to be based on the use of measurement data obtained from the system. This may be particularly significant in the prevention of various illnesses and obesity, as well as in maintaining a good state of health.

Abstract translation: 本发明描述的方法允许通过兼性厌氧细菌调节小肠和整个肠道的pH条件和微生物平衡的新方法，使得该调节可以基于使用从 系统。 这可能在预防各种疾病和肥胖以及维持健康状况方面尤其重要。