公开(公告)号：WO2010042478A2

公开(公告)日：2010-04-15

申请号：PCT/US2009059643

申请日：2009-10-06

Applicant: UNIV ARIZONA ,  HOLL MARK ,  HOUKAL JEFF ,  MARTINEAU RHETT ,  BESSETTE GREG ,  VANNELA RAVEENDER ,  ZHOU CHAO ,  KIM HYUN WOO ,  SHENG JIE ,  SADAYANDI SINDHUJA ,  BANK DANIEL ,  VELA JUAN ,  RITTMANN BRUCE ,  WESTERHOFF PAUL ,  MELDRUM DEIRDRE

Inventor： HOLL MARK ,  HOUKAL JEFF ,  MARTINEAU RHETT ,  BESSETTE GREG ,  VANNELA RAVEENDER ,  ZHOU CHAO ,  KIM HYUN WOO ,  SHENG JIE ,  SADAYANDI SINDHUJA ,  BANK DANIEL ,  VELA JUAN ,  RITTMANN BRUCE ,  WESTERHOFF PAUL ,  MELDRUM DEIRDRE

IPC: C12M3/00 ,  C12M1/04

CPC classification number: C12M33/06 ,  C12M21/02 ,  C12M23/04 ,  C12M27/06 ,  C12M31/10 ,  C12M33/00

Abstract: A highly-configurable, modular system for scale-up studies of photosynthetic microorganisms to produce products such as biofuels includes various components that can be altered or configured to change parameters within the system.

Abstract translation: 用于放大研究光合微生物以生产诸如生物燃料的产品的高度可配置的模块化系统包括可以改变或配置以改变系统内的参数的各种组分。

公开(公告)号：WO1997039338A1

公开(公告)日：1997-10-23

申请号：PCT/US1997005245

申请日：1997-03-31

Applicant: UNIVERSITY OF WASHINGTON ,  WEIGL, Bernhard, H. ,  YAGER, Paul ,  BRODY, James, P. ,  HOLL, Mark, R. ,  KENNY, Margaret ,  SCHUTTE, David ,  HIXSON, Gregory ,  ZEBERT, M., Diane ,  KAMHOLZ, Andrew ,  WU, Caicai ,  ALTENDORF, Eric

Inventor： UNIVERSITY OF WASHINGTON

IPC: G01N21/64

CPC classification number: B01F5/0403 ,  B01F13/0059 ,  B01F13/0093 ,  B01J19/0093 ,  B01J2219/00828 ,  B01J2219/00831 ,  B01J2219/00952 ,  B01L3/502776 ,  B01L2200/0636 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/0487 ,  G01N15/14 ,  G01N30/0005 ,  G01N30/38 ,  G01N30/6095 ,  G01N30/74 ,  G01N35/085 ,  Y10S366/01 ,  Y10T436/25375 ,  Y10T436/2575

Abstract: A channel-cell system is provided for detecting the presence and/or measuring the presence of analyte particles in a sample stream comprising a laminar flow channel (100), two inlet means (30, 20) in fluid connection with said laminar flow channel (100) for respectively conducting into the laminar flow channel (100) an indicator stream (70) which may comprise an indicator substance which indicates the presence of said analyte particles by a detectable change in property when contacted with said analyte particles, and the sample stream (80), wherein the laminar flow channel (100) has a depth sufficiently small to allow laminar flow of the streams and a length sufficient to allow particles of the analyte to diffuse into said indicator stream (70) to the substantial exclusion of said larger particles in the sample stream (80) to form a detection area; and outlet means (60) for conducting the streams out of the laminar flow channel (100) to form a single mixed stream.

Abstract translation: 提供了一种通道细胞系统，用于检测样品流中存在和/或测量分析物颗粒的存在，所述样品流包括层流通道（100），与所述层流通道流体连接的两个入口装置（30,20） 100），用于分别导入层流通道（100），指示物流（70），其可以包含指示物质，其指示当与所述分析物颗粒接触时通过可检测的性质变化指示所述分析物颗粒的存在，并且样品流 （80），其中所述层流通道（100）的深度足够小以允许所述流的层流，并且足够长度以允许所述分析物的颗粒扩散到所述指示物流（70）中，从而基本排除所述较大的 颗粒在样品流（80）中形成检测区域; 以及用于将流引导出层流通道（100）以形成单个混合流的出口装置（60）。

公开(公告)号：WO9618486A3

公开(公告)日：1996-08-29

申请号：PCT/US9516370

申请日：1995-12-12

Applicant: UNIV WASHINGTON ,  KUMAR VIPIN ,  SCHIRMER HENRY G ,  HOLL MARK ROLAND

Inventor： KUMAR VIPIN ,  SCHIRMER HENRY G ,  HOLL MARK ROLAND

IPC: B29C44/34

CPC classification number: B29C44/3453 ,  B29C44/3446 ,  B29C44/348 ,  C08J9/122 ,  C08J9/34 ,  C08J2201/032 ,  C08J2203/06 ,  C08J2205/044 ,  C08J2205/052 ,  C08J2325/06 ,  C08J2327/06 ,  C08J2333/12 ,  C08J2355/02 ,  C08J2367/02 ,  C08J2369/00 ,  Y10S264/13 ,  Y10S264/83

Abstract: The present invention provides a method for the semi-continuous production of microcellular foam articles. In a preferred embodiment, a roll of polymer sheet is provided with a gas channelling means interleaved between the layers of polymer. The roll is exposed to a non-reacting gas at elevated pressure for a period of time sufficient to achieve a desired concentration of gas within the polymer. The saturated polymer sheet is then separated from the gas channelling means and bubble nucleation and growth is initiated by heating the polymer sheet. After foaming, bubble nucleation and growth is quenched by cooling the foamed polymer sheet.

公开(公告)号：WO2010022391A2

公开(公告)日：2010-02-25

申请号：PCT/US2009054781

申请日：2009-08-24

Applicant: AZTE ARIZONA TECHNOLOGY ENTPR ,  UNIV WASHINGTON ,  HOLL MARK ,  MELDRUM DEIRDRE ,  ANIS YASSIR ,  ASHILI SHASHANKA ,  HOUKAL JEFF ,  JOHNSON ROGER ,  KELBAUSKAS LAIMONAS ,  LI YONGZHONG ,  MERZA SAEED ,  NANDAKUMAR VIVEK ,  SMITH DEAN ,  YOUNG CODY ,  TIAN XANQING ,  ZHU HAIXIN ,  CHAO JOSEPH

Inventor： HOLL MARK ,  MELDRUM DEIRDRE ,  ANIS YASSIR ,  ASHILI SHASHANKA ,  HOUKAL JEFF ,  JOHNSON ROGER ,  KELBAUSKAS LAIMONAS ,  LI YONGZHONG ,  MERZA SAEED ,  NANDAKUMAR VIVEK ,  SMITH DEAN ,  YOUNG CODY ,  TIAN XANQING ,  ZHU HAIXIN ,  CHAO JOSEPH

IPC: G01N33/48 ,  C12Q1/68 ,  G01N35/00

CPC classification number: G01N35/0092 ,  C12M41/36 ,  C12M41/38 ,  C12M41/48

Abstract: The present invention provides for an automated, high-throughput, multi-parameter integrated analysis system to monitor, in parallel, cellular parameters descriptive of cell function and associated physiology both singly and in interactions with other cells in tissues. Parameters may be measured with cell or tissue samples confined in arrays of chambers microfabricated in cassettes. System variants provide for cell or tissue harvesting for (optionally destructive) further analysis. The present invention relates to a system for biological laboratory automation, providing for full automation of cell manipulation using capillary micropipettes and associated robotics. This invention provides a robust and flexible technology that can be deployed in research and corporate laboratories, enabling new experimental protocols. Applications include microinjection and high-throughput, single- to multi-cell, multi-parameter monitoring of parameters such as respiration rates, moiety of interest concentrations, metabolic fluxes, chemotactic gradients, gene expression and transcription, translation to proteins (intracellular, membrane, and extracellular), membrane integrity, and cellular ion gradients.

公开(公告)号：WO1998010463A1

公开(公告)日：1998-03-12

申请号：PCT/US1996014342

申请日：1996-09-05

Applicant: REGENTS OF THE UNIVERSITY OF MICHIGAN ,  LITZ, Kyle, Erik ,  HOLL, Mark, M., Banaszak ,  BENDER, John, E., IV

Inventor： REGENTS OF THE UNIVERSITY OF MICHIGAN

IPC: H01L21/20

CPC classification number: C07F7/003 ,  C07F7/10 ,  C30B31/00 ,  H01L21/3115 ,  H01L21/316

Abstract: The present invention provides germanium-containing compounds which can function as dopants and where the methods for their use are flexible, reliable and environmentally safe. The process includes the ability to make bisamidegermanes under relatively mild conditions, usually standard temperature and pressure, without toxic by-products, giving pure products at satisfactory yields.

Abstract translation: 本发明提供了可用作掺杂剂的含锗化合物，其使用方法是灵活的，可靠的和环境安全的。 该方法包括在相对温和的条件（通常是标准温度和压力）下制备双酰胺木糖的能力，没有有毒的副产物，产生满意的产率的纯产品。

公开(公告)号：WO2010075423A3

公开(公告)日：2010-11-18

申请号：PCT/US2009069257

申请日：2009-12-22

Applicant: UNIV MICHIGAN ,  BAKER JR JAMES R ,  BANASZAK HOLL MARK M ,  CHENG XUE-MIN ,  HUANG BAOHUA ,  MCNERNY DANIEL ,  MULLEN DOUGLAS G ,  THOMAS THOMMEY

Inventor： BAKER JR JAMES R ,  BANASZAK HOLL MARK M ,  CHENG XUE-MIN ,  HUANG BAOHUA ,  MCNERNY DANIEL ,  MULLEN DOUGLAS G ,  THOMAS THOMMEY

IPC: A61K47/48 ,  A61K31/7088 ,  A61K39/385 ,  A61K39/395 ,  A61P19/00 ,  A61P29/00

CPC classification number: A61K31/74 ,  A61K31/00 ,  A61K45/06 ,  A61K47/551 ,  A61K47/59 ,  A61K47/595 ,  C08G73/0206 ,  C08G73/028 ,  C08G81/00 ,  C08G83/003 ,  C08L79/02 ,  C08L101/005 ,  A61K2300/00

Abstract: The present invention relates to novel therapeutic and diagnostic dendrimer based modular platforms (e.g., drug delivery platforms). In particular, the dendrimer based modular platforms are configured such that two or more dendrimers (e.g., PAMAM dendrimers) are coupled together (e.g., via a cycloaddition reaction) wherein each of the coupled dendrimers is functionalized (e.g., functionalized for targeting, imaging, sensing, and/or providing a therapeutic or diagnostic material and/or monitoring response to therapy). In some embodiments, the present invention provides dendrimer based modular platforms having coupled dendrimers (e.g., two or more coupled dendrimers) wherein each dendrimer is conjugated to one or more functional groups (e.g., therapeutic agent, imaging agent, targeting agent, triggering agent) (e.g., for specific targeting and/or therapeutic use of the dendrimer based modular platform). In some embodiments, the functional groups are conjugated to the dendrimers via a linker and/or a triggering agent. In addition, the present invention is directed to methods of synthesizing dendrimer based modular platforms, compositions comprising the dendrimer based modular platforms, as well as systems and methods utilizing the dendrimer based modular platforms (e.g., in diagnostic and/or therapeutic settings (e.g., for the delivery of therapeutics, imaging, and/or targeting agents (e.g., in disease (e.g., cancer) diagnosis and/or therapy, etc.)).

Abstract translation: 本发明涉及新颖的基于治疗和诊断树枝状大分子的模块化平台（例如药物递送平台）。 特别地，基于树枝状聚合物的模块化平台被构造成使得两种或更多种树枝状聚合物（例如，PAMAM树枝状聚合物）偶联在一起（例如，经由环加成反应），其中每个偶联的树枝状聚合物被官能化（例如官能化用于靶向，成像， 感测和/或提供对治疗的治疗或诊断材料和/或监测响应）。 在一些实施方案中，本发明提供了具有偶联树枝状聚合物（例如两种或更多种偶联树枝状聚合物）的基于树枝状聚合物的模块化平台，其中每种树枝状聚合物缀合至一种或多种官能团（例如治疗剂，成像剂，靶向剂，引发剂） （例如，用于基于树状聚合物的模块化平台的特定靶向和/或治疗用途）。 在一些实施方案中，官能团通过接头和/或触发剂与树枝状聚合物缀合。 另外，本发明涉及合成基于树枝状聚合物的模块化平台的方法，包含基于树状聚合物的模块化平台的组合物，以及利用基于树状聚合物的模块化平台的系统和方法（例如，在诊断和/或治疗设置（例如， 用于递送治疗剂，成像剂和/或靶向剂（例如在疾病（例如癌症）诊断和/或治疗中等））。

公开(公告)号：WO2010022391A9

公开(公告)日：2010-04-15

申请号：PCT/US2009054781

申请日：2009-08-24

Applicant: AZTE ARIZONA TECHNOLOGY ENTPR ,  UNIV WASHINGTON ,  HOLL MARK ,  MELDRUM DEIRDRE ,  ANIS YASSIR ,  ASHILI SHASHANKA ,  HOUKAL JEFF ,  JOHNSON ROGER ,  KELBAUSKAS LAIMONAS ,  LI YONGZHONG ,  MERZA SAEED ,  NANDAKUMAR VIVEK ,  SMITH DEAN ,  YOUNG CODY ,  TIAN XANQING ,  ZHU HAIXIN ,  CHAO JOSEPH

Inventor： HOLL MARK ,  MELDRUM DEIRDRE ,  ANIS YASSIR ,  ASHILI SHASHANKA ,  HOUKAL JEFF ,  JOHNSON ROGER ,  KELBAUSKAS LAIMONAS ,  LI YONGZHONG ,  MERZA SAEED ,  NANDAKUMAR VIVEK ,  SMITH DEAN ,  YOUNG CODY ,  TIAN XANQING ,  ZHU HAIXIN ,  CHAO JOSEPH

IPC: G01N33/48 ,  C12Q1/68 ,  G01N35/00

CPC classification number: G01N35/0092 ,  C12M41/36 ,  C12M41/38 ,  C12M41/48

Abstract: The present invention provides for an automated, high-throughput, multi-parameter integrated analysis system to monitor, in parallel, cellular parameters descriptive of cell function and associated physiology both singly and in interactions with other cells in tissues. Parameters may be measured with cell or tissue samples confined in arrays of chambers microfabricated in cassettes. System variants provide for cell or tissue harvesting for (optionally destructive) further analysis. The present invention relates to a system for biological laboratory automation, providing for full automation of cell manipulation using capillary micropipettes and associated robotics. This invention provides a robust and flexible technology that can be deployed in research and corporate laboratories, enabling new experimental protocols. Applications include microinjection and high-throughput, single- to multi-cell, multi-parameter monitoring of parameters such as respiration rates, moiety of interest concentrations, metabolic fluxes, chemotactic gradients, gene expression and transcription, translation to proteins (intracellular, membrane, and extracellular), membrane integrity, and cellular ion gradients.

Abstract translation: 本发明提供了一种自动化，高吞吐量的多参数综合分析系统，以并行地监测描述细胞功能和相关生理学的细胞参数，其单独地和与组织中的其他细胞相互作用。 参数可以用限制在盒中的微室的阵列中的细胞或组织样品来测量。 系统变体提供细胞或组织采集（可选择的破坏性）进一步分析。 本发明涉及用于生物实验室自动化的系统，提供使用毛细管微量移液管和相关机器人的细胞操作的全自动化。 本发明提供了可以在研究和公司实验室中部署的强大而灵活的技术，从而实现了新的实验方案。 应用包括显微注射和高通量，单细胞，多参数监测参数，如呼吸速率，感兴趣浓度部分，代谢通量，趋化性梯度，基因表达和转录，翻译成蛋白质（细胞内，膜， 和细胞外），膜完整性和细胞离子梯度。

公开(公告)号：WO1996018486A2

公开(公告)日：1996-06-20

申请号：PCT/US1995016370

申请日：1995-12-12

Applicant: UNIVERSITY OF WASHINGTON ,  KUMAR, Vipin ,  SCHIRMER, Henry, G. ,  HOLL, Mark, Roland

Inventor： UNIVERSITY OF WASHINGTON

IPC: B29C44/34

CPC classification number: B29C44/3453 ,  B29C44/3446 ,  B29C44/348 ,  C08J9/122 ,  C08J9/34 ,  C08J2201/032 ,  C08J2203/06 ,  C08J2205/044 ,  C08J2205/052 ,  C08J2325/06 ,  C08J2327/06 ,  C08J2333/12 ,  C08J2355/02 ,  C08J2367/02 ,  C08J2369/00 ,  Y10S264/13 ,  Y10S264/83

Abstract: The present invention provides a method for the semi-continuous production of microcellular foam articles. In a preferred embodiment, a roll of polymer sheet is provided with a gas channelling means interleaved between the layers of polymer. The roll is exposed to a non-reacting gas at elevated pressure for a period of time sufficient to achieve a desired concentration of gas within the polymer. The saturated polymer sheet is then separated from the gas channelling means and bubble nucleation and growth is initiated by heating the polymer sheet. After foaming, bubble nucleation and growth is quenched by cooling the foamed polymer sheet.

Abstract translation: 本发明提供了一种半连续生产微孔泡沫制品的方法。 在一个优选实施例中，一卷聚合物片材设置有交错在聚合物层之间的气体通道装置。 将辊在升高的压力下暴露于非反应性气体一段足以在聚合物内达到所需浓度的气体的时间。 然后将饱和聚合物片材与气体引导装置分离，并且气泡成核，并通过加热聚合物片材开始生长。 发泡后，通过冷却发泡聚合物片来淬灭气泡成核和生长。

公开(公告)号：WO2011028334A2

公开(公告)日：2011-03-10

申请号：PCT/US2010043109

申请日：2010-07-23

Applicant: UNIV MICHIGAN ,  BAKER JR JAMES R ,  BANASZAK HOLL MARK M ,  MULLEN DOUGLAS GURNETT ,  ORR BRADFORD G ,  DESAI ANKUR ,  SANDER LEONARD M ,  WADDELL JACK NEEL

Inventor： BAKER JR JAMES R ,  BANASZAK HOLL MARK M ,  MULLEN DOUGLAS GURNETT ,  ORR BRADFORD G ,  DESAI ANKUR ,  SANDER LEONARD M ,  WADDELL JACK NEEL

IPC: A61K31/74 ,  A61K9/20 ,  A61K31/4985 ,  A61K47/34 ,  A61K47/48 ,  A61P35/00

CPC classification number: C08G83/004

Abstract: The present invention relates to novel methods of synthesis and isolation of dendrimer systems. In particular, the present invention is directed to novel dendrimer conjugates with defined and limited numbers of ligand conjugates and high levels of structural uniformity, methods of synthesizing the same, compositions comprising the conjugates, as well as systems and methods utilizing the conjugates (e.g., in diagnostic and/or therapeutic settings (e.g., for the delivery of therapeutics, imaging, and/or targeting agents (e.g., in disease (e.g., cancer) diagnosis and/or therapy, pain therapy, etc.)).

Abstract translation: 本发明涉及树枝状大分子体系的合成和分离的新方法。 特别地，本发明涉及具有限定数量和限定数量的配体缀合物和高水平结构均匀性的新型树状聚合物缀合物，其合成方法，包含缀合物的组合物以及利用缀合物的系统和方法（例如， 在诊断和/或治疗设置（例如，用于递送治疗剂，成像和/或靶向剂（例如，在疾病（例如，癌症）诊断和/或治疗，疼痛治疗等中））。

公开(公告)号：WO2010075423A2

公开(公告)日：2010-07-01

申请号：PCT/US2009/069257

申请日：2009-12-22

Applicant: THE REGENTS OF THE UNIVERSITY OF MICHIGAN ,  BAKER, JR., James R. ,  BANASZAK HOLL, Mark M. ,  CHENG, Xue-Min ,  HUANG, Baohua ,  MCNERNY, Daniel ,  MULLEN, Douglas G. ,  THOMAS, Thommey

Inventor： BAKER, JR., James R. ,  BANASZAK HOLL, Mark M. ,  CHENG, Xue-Min ,  HUANG, Baohua ,  MCNERNY, Daniel ,  MULLEN, Douglas G. ,  THOMAS, Thommey

IPC: A61K47/48 ,  A61K39/385 ,  A61K39/395 ,  A61K31/7088 ,  A61P29/00 ,  A61P19/00

CPC classification number: A61K31/74 ,  A61K31/00 ,  A61K45/06 ,  A61K47/551 ,  A61K47/59 ,  A61K47/595 ,  C08G73/0206 ,  C08G73/028 ,  C08G81/00 ,  C08G83/003 ,  C08L79/02 ,  C08L101/005 ,  A61K2300/00

Abstract: The present invention relates to novel therapeutic and diagnostic dendrimer based modular platforms (e.g., drug delivery platforms). In particular, the dendrimer based modular platforms are configured such that two or more dendrimers (e.g., PAMAM dendrimers) are coupled together (e.g., via a cycloaddition reaction) wherein each of the coupled dendrimers is functionalized (e.g., functionalized for targeting, imaging, sensing, and/or providing a therapeutic or diagnostic material and/or monitoring response to therapy). In some embodiments, the present invention provides dendrimer based modular platforms having coupled dendrimers (e.g., two or more coupled dendrimers) wherein each dendrimer is conjugated to one or more functional groups (e.g., therapeutic agent, imaging agent, targeting agent, triggering agent) (e.g., for specific targeting and/or therapeutic use of the dendrimer based modular platform). In some embodiments, the functional groups are conjugated to the dendrimers via a linker and/or a triggering agent. In addition, the present invention is directed to methods of synthesizing dendrimer based modular platforms, compositions comprising the dendrimer based modular platforms, as well as systems and methods utilizing the dendrimer based modular platforms (e.g., in diagnostic and/or therapeutic settings (e.g., for the delivery of therapeutics, imaging, and/or targeting agents (e.g., in disease (e.g., cancer) diagnosis and/or therapy, etc.)).

Abstract translation: 本发明涉及新颖的基于树枝状大分子的模块化平台（例如药物递送平台）。 特别地，基于树枝状聚合物的模块化平台被配置为使得两个或更多个树枝状聚合物（例如，PAMAM树枝状聚合物）偶联在一起（例如，通过环加成反应），其中每个偶联的树枝状聚合物被官能化（例如，功能化用于靶向，成像， 感测和/或提供治疗或诊断材料和/或监测对治疗的响应）。 在一些实施方案中，本发明提供了具有结合的树枝状大分子（例如两个或更多个偶联的树枝状大分子）的树枝状大分子模块化平台，其中每个树枝状聚合物与一个或多个官能团（例如，治疗剂，成像剂，靶向剂，引发剂） （例如，用于基于树枝状聚合物的模块化平台的特异性靶向和/或治疗用途）。 在一些实施方案中，官能团通过接头和/或引发剂与树枝状聚合物缀合。 此外，本发明涉及基于树枝状聚合物的模块化平台的方法，包含基于树枝状聚合物的模块化平台的组合物以及利用基于树枝状聚合物的模块化平台的系统和方法（例如，在诊断和/或治疗设置（例如， 用于递送治疗剂，成像和/或靶向剂（例如，在疾病（例如癌症）诊断和/或治疗中等））。