公开(公告)号：WO2023064904A1

公开(公告)日：2023-04-20

申请号：PCT/US2022/078120

申请日：2022-10-14

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  CHARITÉ – UNIVERSITÄTSMEDIZIN BERLIN ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： GROSSWENDT, Stefanie ,  MEISSNER, Alexander ,  CHRISTALLER, Tobias ,  STACHEL-BRAUM, Philipp ,  GARTNER, Zev ,  MOSER, Brittany ,  CHOW, Eric

IPC: C40B40/06 ,  C12Q1/6837 ,  C12Q1/6811 ,  C40B20/04

Abstract: The present invention provides a method for obtaining sequencing-based information from cells/nuclei, the method comprising (a) a first barcoding of cells and/or nuclei of a group of cells and/or nuclei comprising interacting cells with one or more group-specific barcode sequence(s) or a group-specific combination of one or more barcode sequence(s); (b) a second barcoding of nucleic acid molecules contained in and/or attached to individual cells and/or nuclei from said group of cells/nuclei with a cell/nucleus-specific barcode sequence; and (c) sequencing of the barcoded nucleic acid sequences.

公开(公告)号：WO2019051072A1

公开(公告)日：2019-03-14

申请号：PCT/US2018/049728

申请日：2018-09-06

Applicant: REGENTS OF THE UNIVERSITY OF MINNESOTA ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

Inventor： KODANDARAMAIAH, Suhasa ,  TAVERNA, Elena ,  SHULL, Gabriella ,  HUTTNER, Wieland

IPC: C12M1/42 ,  C12M1/00 ,  C12M1/36

CPC classification number: C12M21/08 ,  C12M23/50 ,  C12M33/04 ,  C12M35/00 ,  C12M41/48

Abstract: Techniques are described for automated microinjection of substances, such as genetic material, into single cells in tissue samples. An example system comprises a robotic manipulator apparatus configured to hold and position a micropipette. Furthermore, the system comprises a microscope camera positioned to observe an injection site. A computing device receives image data from a microscope camera of the system, where the image data represents an image of a tissue sample. The computing device receives, via a user interface, an indication of a line traced by a user on the image of a tissue sample. In response, the computing device controls the robotic manipulator apparatus to move a tip of the micropipette along a path defined by the trajectory line. The pressure controller injects a gas into the micropipette to eject a substance out of the micropipette at one or more points along the path defined by the trajectory line.

公开(公告)号：WO2016038108A1

公开(公告)日：2016-03-17

申请号：PCT/EP2015/070647

申请日：2015-09-09

Applicant: UNIVERSITEIT LEIDEN ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

Inventor： FAEZ, Sanli Ebrahimi Pour ,  SANDOGHDAR, Vahid

IPC: G01N15/14 ,  B01L3/00 ,  G01N21/03

CPC classification number: G01N15/1436 ,  B01L3/502761 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/474 ,  G01N21/53 ,  G01N21/6458 ,  G01N2015/0038 ,  G01N2021/4742 ,  G01N2201/08

Abstract: There is provided a particle detection apparatus (30) comprising: a channel (32) including an inlet and at least one channel wall, the inlet permitting light to be introduced into the channel (32), the or each channel wall being arranged to define a channel path through which light may propagate; a light source (34) configured to introduce light into the channel (32) via the inlet, the channel (32) being shaped to guide the light to propagate along the channel path for illuminating a particle or a plurality of particles located in the channel path; and a monitoring device (36) configured to detect scattered light that is created by the illumination of the or each particle by the guided light and that leaves the channel (32) by passing through the or each channel wall.

Abstract translation: 提供了一种颗粒检测装置（30），包括：通道（32），其包括入口和至少一个通道壁，所述入口允许光引入通道（32），所述或每个通道壁被布置成限定 光可以传播的通道; 经配置以经由所述入口将光引入所述通道（32）的光源（34），所述通道（32）被成形为引导所述光沿所述通道路径传播以照射位于所述通道中的颗粒或多个颗粒 路径; 以及监测装置（36），其被配置为检测由所述引导光照射所述颗粒或每个颗粒产生的散射光，并且通过所述通道壁或每个通道壁离开所述通道（32）。

公开(公告)号：WO2014170401A1

公开(公告)日：2014-10-23

申请号：PCT/EP2014/057806

申请日：2014-04-16

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V.

Inventor： MIRZOYAN, Razmik ,  TESHIMA, Masahiro ,  POPOVA, Elena

IPC: H01L31/107 ,  H01L31/0224 ,  H01L27/144

CPC classification number: H01L27/1446 ,  H01L31/022408 ,  H01L31/107 ,  H01L31/1804

Abstract: The silicon-based photomultiplier device comprises a substrate (1), a first layer (2) of a first conductivity type, a second layer (3) of a second conductivity type formed on the first layer, wherein the first layer (2) and the second layer (3) form a p-n junction, wherein the first layer (2) and the second layer (3) are disposed on or above the substrate (1). A material layer (15) between the substrate (1) and the first layer (2) fulfils the function of a light absorber, thereby efficiently suppressing crosstalk between adjacent cells of the device. Material layer (15) may further serve as an electrode for readout of electrical signals from the device.

Abstract translation: 所述硅基光电倍增器装置包括基板（1），第一导电类型的第一层（2）和形成在所述第一层上的第二导电类型的第二层（3），其中所述第一层（2）和 第二层（3）形成pn结，其中第一层（2）和第二层（3）设置在衬底（1）上或上方。 基板（1）与第一层（2）之间的材料层（15）实现光吸收体的功能，从而有效地抑制装置的相邻单元之间的串扰。 材料层（15）还可以用作用于从装置读出电信号的电极。

公开(公告)号：WO2013078413A1

公开(公告)日：2013-05-30

申请号：PCT/US2012/066394

申请日：2012-11-21

Applicant: THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPARTMENT OF HEALTH AND HUMAN SERVICES ,  MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  UNIVERSITY OF MARYLAND, BALTIMORE

Inventor： BOXER, Matthew B. ,  BELLER, Mathias ,  TSCHAPALDA, Kirsten ,  SHEN, Min ,  LIU, Li ,  ZHANG, Yaqin ,  LI, Zhuyin ,  SZTALRYD, Carole

IPC: C07D231/56 ,  A61K31/416 ,  A61P3/00 ,  A61P3/04 ,  A61P3/06 ,  A61P3/10 ,  A61P9/10 ,  A61P9/12 ,  A61P9/04

CPC classification number: C07D231/56

Abstract: Disclosed are compounds of formulas I, II, and III: Formulas I, II, and III wherein R 1 -R 19 and n are as described herein, or pharmaceutically acceptable salts thereof, or an enantiomer thereof or a conjugate thereof wherein the conjugate comprises a marker group, for use in treating or preventing a disease or disorder responsive to reduction of lipid storage droplets in an animal in need thereof. Also disclosed are pharmaceutical compositions comprising such compounds or pharmaceutically acceptable salts thereof and a pharmaceutically acceptable carrier and methods of treating or preventing neurodegenerative diseases and disorders.

Abstract translation: 公开了式I，II和III的化合物：式I，II和III，其中R 1 -R 19和n如本文所述，或其药学上可接受的盐，或其对映异构体或其缀合物，其中缀合物包含标记 用于治疗或预防对有需要的动物中的脂质储存液滴的减少有反应的疾病或病症。 还公开了包含这些化合物或其药学上可接受的盐和药学上可接受的载体的药物组合物以及治疗或预防神经变性疾病和病症的方法。

公开(公告)号：WO2013056720A1

公开(公告)日：2013-04-25

申请号：PCT/EP2011/005231

申请日：2011-10-18

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  HELL, Stefan, W. ,  NIZAMOV, Shamil ,  DONNERT, Gerald ,  KOLMAKOV, Kirill ,  SCHILL, Heiko ,  KASTRUP, Lars ,  WURM, Christian, A. ,  BELOV, Vladimir, N. ,  BUECKERS, Johanna ,  WILLIG, Katrin

Inventor： HELL, Stefan, W. ,  NIZAMOV, Shamil ,  DONNERT, Gerald ,  KOLMAKOV, Kirill ,  SCHILL, Heiko ,  KASTRUP, Lars ,  WURM, Christian, A. ,  BELOV, Vladimir, N. ,  BUECKERS, Johanna ,  WILLIG, Katrin

IPC: C09B11/24 ,  C09B57/02 ,  G01N33/58 ,  C09B57/10

CPC classification number: G01N33/52 ,  C09B11/24 ,  C09B57/02 ,  C09B57/10 ,  G01N33/533 ,  G01N33/582 ,  G01N2458/00

Abstract: The invention relates to novel fluorescent dyes with phosphorylated hydroxymethyl groups, a method for preparing the same as well as to their use in imaging techniques. Said fluorescent dyes are coumarin, rhodamine or BODIPY dyes having of one of the following general formulae (I-III) wherein W = OP(0)Y 1 Y 2 or P(0)Υ 1 Υ 2 , where Y 1 and Y 2 independently denote any of the following residues: OH, O(-), OR a and OR b , NHR a and NHR b , NR a R b and NR c R d , OR a and NHR b , OR a and NR b R c , NHR a and NR b R c ; and any salt thereof.

Abstract translation: 本发明涉及具有磷酸化羟甲基的新型荧光染料，其制备方法以及其在成像技术中的应用。 所述荧光染料是具有以下通式（I-III）之一的香豆素，罗丹明或BODIPY染料，其中W = OP（O）Y1Y2或P（0）Upsilon1Upsilon2，其中Y1和Y2独立地表示以下任何残基： OH，O（ - ），ORa和ORb，NHRa和NHRb，NRaRb和NRcRd，ORa和NHRb，ORa和NRbRc，NHRa和NRbRc; 及其任何盐。

公开(公告)号：WO2012016709A1

公开(公告)日：2012-02-09

申请号：PCT/EP2011/003934

申请日：2011-08-04

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  FOERCH, Renate ,  DUQUE, Luis ,  LOTZ, Alexander

Inventor： FOERCH, Renate ,  DUQUE, Luis ,  LOTZ, Alexander

IPC: A61L27/34 ,  A61L29/08 ,  A61L31/10

CPC classification number: A61L27/34 ,  A61L27/54 ,  A61L31/10 ,  A61L31/16 ,  A61L2300/102 ,  A61L2300/404 ,  A61L2400/18

Abstract: The present invention provides a multifunctional coating having antimicrobial activity and the capability to regulate cell adhesion, comprising, deposited on a substrate surface, i) an optional anchoring layer, ii) a Zn-release layer deposited on the anchoring layer or directly on the substrate surface and iii) a functional barrier layer deposited on the Zn-release layer, wherein the functional barrier layer is capable to slow down the diffusion of Zn from the Zn-release layer through the barrier layer and wherein the surface of the functional barrier layer regulates cell adhesion. In a more specific embodiment of the invention, the polymeric film of the Zn-release layer has been formed by plasma assisted polymerisation of zinc-organic precursors, e.g. Zn (acac) 2 , bis (pentafluoro-phenyl ) -zinc, and zinc-hexafluoro acetylacetonate.

Abstract translation: 本发明提供了具有抗微生物活性和调节细胞粘附能力的多功能涂层，其包括沉积在基材表面上，i）任选的锚定层，ii）沉积在锚定层上或直接沉积在基底上的Zn释放层 表面和iii）沉积在所述Zn释放层上的功能性阻挡层，其中所述功能阻挡层能够减缓Zn从Zn释放层通过势垒层的扩散，并且其中功能性阻挡层的表面调节 细胞粘附。 在本发明的一个更具体的实施方案中，Zn释放层的聚合物膜是通过锌 - 有机前体的等离子体辅助聚合形成的。 Zn（acac）2，双（五氟 - 苯基）锌和六氟乙酰丙酮锌。

公开(公告)号：WO2011144344A2

公开(公告)日：2011-11-24

申请号：PCT/EP2011/002506

申请日：2011-05-19

Applicant: MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  ADTEC EUROPE LTD. ,  MORFILL Gregor ,  SHIMIZU, Tetsuji ,  LI, Yang-Fang ,  URAYAMA, Takuya ,  ZIMMERMANN Julia ,  STEFFES Bernd

Inventor： MORFILL Gregor ,  SHIMIZU, Tetsuji ,  LI, Yang-Fang ,  URAYAMA, Takuya ,  ZIMMERMANN Julia ,  STEFFES Bernd

IPC: A45D34/02 ,  A45D34/04 ,  A47L15/00 ,  A61L2/14 ,  H05H1/24 ,  H05H1/30 ,  H05H1/34 ,  A61N1/44 ,  B66B31/02

CPC classification number: A61L2/14 ,  A01J7/04 ,  A45D34/041 ,  A47L15/4236 ,  A61B1/122 ,  A61B18/14 ,  A61C19/06 ,  B62B5/069 ,  B65B55/10 ,  D06F35/003 ,  H05H1/2406 ,  H05H2001/2418 ,  H05H2245/1225

Abstract: The invention relates to an appliance (18) for at least partially disinfecting/sterilising a contaminated surface (21), wherein the appliance (18) comprises an integrated plasma source for at least partially disinfecting/sterilising the surface by generating a non-thermal atmospheric plasma on the surface thereby reducing the concentration of pathogenic germs on the surface.

Abstract translation: 本发明涉及一种用于对受污染表面（21）进行至少部分消毒/灭菌的器具（18），其中所述器具（18）包括用于通过产生非热常压来对所述表面进行至少部分消毒/灭菌的集成等离子体源 表面上的等离子体，从而降低表面上致病细菌的浓度。

公开(公告)号：WO2007006509A1

公开(公告)日：2007-01-18

申请号：PCT/EP2006/006677

申请日：2006-07-07

Applicant: MAX-PLANCK GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  GEORG-AUGUST-UNIVERSITAET GOETTINGEN ,  FAUBEL, Manfred ,  CHARVAT, Ales ,  TROE, Juergen ,  ABEL, Bernd

Inventor： FAUBEL, Manfred ,  CHARVAT, Ales ,  TROE, Juergen ,  ABEL, Bernd

IPC: H01J49/04 ,  B05B1/02

CPC classification number: B05B1/02 ,  H01J49/0431

Abstract: Die Erfindung betrifft eine Düsenanordnung (1) zur Abgabe eines Fluids, insbesondere zur Einspritzung des Fluids in eine Vakuumkammer, mit einem Düsenkörper (2) mit einem durchgehenden Düsenkanal (9) und einer ausgangsseitig ausgebildeten Düsenöffnung (10) zur Abgabe des Fluids sowie mit einer koaxial zu dem Düsenkanal (9) verlaufenden Zuleitungskapillare (8) zur Zuführung des einzuspritzenden Fluids. Es wird vorgeschlagen, dass die Zuleitungskapillare (8) in Strömungsrichtung bis in den Düsenkanal (9) des Düsenkörpers (2) hinein geführt ist, um das Totvolumen zu verringern.

Abstract translation: 本发明涉及一种喷嘴装置（1）用于分配流体，特别是用于流体注入到真空室中，具有喷嘴主体（2）具有用于分配流体，并用连续的喷嘴通道（9）和输出侧形成喷嘴开口（10） 同轴延伸至喷嘴通道（9）Zuleitungskapillare（8），用于将流体供应至喷射。 它提出，Zuleitungskapillare（8）中流动到喷嘴主体（2）的喷嘴通道（9）的方向，以减小死体积引导。

公开(公告)号：WO2003026562A2

公开(公告)日：2003-04-03

申请号：PCT/IL2002/000786

申请日：2002-09-24

Applicant: YEDA RESEARCH AND DEVELOPMENT CO. LTD. ,  MAX PLANCK SOCIETY MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V. ,  YONATH, Ada ,  BASHAN, Anat ,  ZARIVACH, Raz ,  FRANCESCHI, Francois ,  SCHLUENZEN, Frank ,  HARMS, Joerg ,  ALBRECHT, Renate ,  BERISIO, Rita

Inventor： YONATH, Ada ,  BASHAN, Anat ,  ZARIVACH, Raz ,  FRANCESCHI, Francois ,  SCHLUENZEN, Frank ,  HARMS, Joerg ,  ALBRECHT, Renate ,  BERISIO, Rita

IPC: A61K

CPC classification number: G01N33/6803 ,  C07K2299/00 ,  G01N33/554 ,  G01N2333/195

Abstract: Methods of growing crystals of free, antibiotic complexed, or ribosomal substrate complexed large ribosomal subunits, coordinates defining the three-dimensional atomic structure thereof and methods of utilizing such coordinates for rational design or identification of antibiotics or large ribosomal subunits having desired characteristics are disclosed.

Abstract translation: 公开了生长游离，抗生素复合物或核糖体底物的晶体的方法，复合了大核糖体亚基，定义其三维原子结构的坐标和利用这种坐标来合理设计或鉴定具有所需特征的抗生素或大核糖体亚基的方法。