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    BARLEY AND USES THEREOF
    3.
    发明申请
    BARLEY AND USES THEREOF 审中-公开
    BARLEY及其使用

    公开(公告)号：WO2011011833A1

    公开(公告)日：2011-02-03

    申请号：PCT/AU2010/000968

    申请日：2010-07-30

    Applicant: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  AUSTRALIAN CAPITAL VENTURES LIMITED ,  LI, Zhongyi ,  MORELL, Matthew Kennedy

    Inventor： LI, Zhongyi ,  MORELL, Matthew Kennedy

    IPC: A01H5/00 ,  A01H4/00 ,  C12N15/09 ,  A01H1/06 ,  C12N5/10 ,  C12Q1/25

    CPC classification number: A01H5/10 ,  C12N15/8245 ,  C12Q1/25

    Abstract: The invention provides barley grain comprising a reduced level or activity of starch synthase Ha protein and a starch content of at least 41% (w/w) and methods of producing, identifying and using same. The grain may comprise an amylase content of at least 50 %, a β-glucan content of 5-9 % (w/w) or greater than 9% (w/w), and/or a fructan content of 3- 11 % (w/w). The fructan may comprise a degree of polymerization from about 3 to about 12. For example, the plant and grain comprises a sex6-292 allele and/or an amol mutation. A food or beverage product, and methods of producing a food or beverage product, comprising obtaining or producing the subject grain and processing the grain to produce the product. Also contemplated are methods of improving one or more indicators of health in a mammal comprising administering a composition comprising the subject barley grain or a product comprising same.

    Abstract translation: 本发明提供了包含降低的淀粉合成酶Ha蛋白的水平或活性和至少41％（w / w）的淀粉含量的麦粒，以及产生，鉴定和使用淀粉合成酶的方法。 颗粒可以包含至少50％的淀粉酶含量，5-9％（w / w）或大于9％（w / w）的β-葡聚糖含量和/或3- 11％的果聚糖含量， （重量/重量）。 果聚糖可以包含约3至约12的聚合度。例如，植物和颗粒包含6-292等位基​​因和/或氨基酸突变。 食品或饮料产品，以及生产食品或饮料产品的方法，包括获得或生产受试者颗粒并加工谷物以生产产品。 还考虑了改善哺乳动物中一种或多种健康指标的方法，包括施用包含受试大麦粒的组合物或包含其的产品。

    BETA GLUCAN EXTRACTION FROM CEREALS
    6.
    发明申请
    BETA GLUCAN EXTRACTION FROM CEREALS 审中-公开
    谷物从谷物中提取葡萄糖

    公开(公告)号：WO0056268A3

    公开(公告)日：2000-12-21

    申请号：PCT/NZ0000034

    申请日：2000-03-22

    Applicant: NZ INST FOR CROP & FOOD RES ,  COLES THEODORE KENNETH ,  MORELL MATTHEW KENNEDY ,  ABBOT REBECCA CLARE ,  COLES GRAEME DOUGLAS

    Inventor： COLES THEODORE KENNETH ,  MORELL MATTHEW KENNEDY ,  ABBOT REBECCA CLARE ,  COLES GRAEME DOUGLAS

    IPC: C08B30/10 ,  C08B37/00

    CPC classification number: C08B37/0024 ,  C08B30/10

    Abstract: The invention discloses a process for separating gelling beta -glucan from cereals. The process involves: 1) extracting a soluble factor from cereal flour into solution, and not extracting beta -glucans into solution; 2) separating the starch from the solid residue remaining after the first step; and 3) adding the extract from the first step to the non starch residue from the second step. The soluble factor causes gelling beta -glucan to be released, from the non starch residue, into solution.

    Abstract translation: 本发明公开了一种从谷物中分离凝胶β-葡聚糖的方法。 该过程包括：1）将谷物粉中的可溶性因子提取到溶液中，不将β-葡聚糖提取到溶液中; 2）将淀粉与第一步残留的固体残渣分离; 和3）从第二步骤将第一步骤的提取物加入至非淀粉残渣中。 可溶性因子使得β-葡聚糖凝胶从非淀粉残渣中释放到溶液中。

    PLANTS WITH MODIFIED STARCH METABOLISM
    8.
    发明申请
    PLANTS WITH MODIFIED STARCH METABOLISM 审中-公开
    具有改性淀粉代谢的植物

    公开(公告)号：WO2009067751A1

    公开(公告)日：2009-06-04

    申请号：PCT/AU2008/001759

    申请日：2008-11-27

    Applicant: COMMONWEALTH SCIENTIFIC AND INDUSTRIAL RESEARCH ORGANISATION ,  GRAINS RESEARCH AND DEVELOPMENT CORPORATION ,  RAL, Jean-Philippe Francois Michel ,  LI, Zhongyi ,  MORELL, Matthew Kennedy

    Inventor： RAL, Jean-Philippe Francois Michel ,  LI, Zhongyi ,  MORELL, Matthew Kennedy

    IPC: C12N15/82 ,  C12N9/00 ,  C12N15/29 ,  A01H5/00 ,  C12N9/12

    CPC classification number: C12N15/8245 ,  C07K14/415 ,  C12N9/1294 ,  C12N15/8234 ,  C12N15/8261 ,  C12Y207/09004

    Abstract: The specification provides methods of obtaining a genetically modified plant which has increased production potential compared to a control plant, the method comprising the steps of i) obtaining a plurality of plants at least one of which comprises in its genome a heterologous polynucleotide, ii) identifying from the plurality of plants a plant which has increased production potential relative to the control plant and comprises the heterologous polynucleotide, and iii) selecting the genetically modified plant, wherein the polynucleotide comprises a transcriptional control sequence operably linked to a nucleic acid sequence which encodes an agent that modifies endogenous starch phosphorylation and/or starch degradation in the plant. In some embodiments, the plant has increased endogenous glycosylase or increased digestibility compared to a control plant. In some specific embodiments, the endogenous starch phosphorylation and/or starch degradation is modified by modifying expression or activity of one or more enzymes selected from the group consisting of α-amylase (EC 3.2.1.1), β-amylase (EC 3.2.1.2), glucoamylase (EC 3.2.1.3), starch phosphorylase (EC2.4.1.1), glycosylase (EC 3.1.33), sucrase-isomaltase (EC 3.2.10), amylomaltase (EC 2.4.1.25), maltase (EC 3.2.1.20), isoamylase, and α-glucan, water dikinase (GWD, EC 2.7.9.4).

    Abstract translation: 本说明书提供了获得与对照植物相比具有增加的生产潜力的遗传修饰植物的方法，所述方法包括以下步骤：i）获得多个植物，其中至少一个在其基因组中包含异源多核苷酸，ii）鉴定 来自多个植物的植物相对于对照植物具有增加的生产潜力并且包含异源多核苷酸，以及iii）选择遗传修饰植物，其中所述多核苷酸包含可操作地连接到编码 修饰植物内源性淀粉磷酸化和/或淀粉降解的试剂。 在一些实施方案中，与对照植物相比，植物具有增加的内源糖基化酶或增加的消化率。 在一些具体实施方案中，通过修饰选自α-淀粉酶（EC 3.2.1.1），β-淀粉酶（EC 3.2.1.2）的一种或多种酶的表达或活性来修饰内源淀粉磷酸化和/或淀粉降解 ），葡糖淀粉酶（EC 3.2.1.3），淀粉磷酸化酶（EC2.4.1.1），糖基化酶（EC 3.1.33），蔗糖酶异麦芽糖酶（EC 3.2.10），淀粉麦芽糖酶（EC 2.4.1.25），麦芽糖酶 1.20），异淀粉酶和α-葡聚糖，水二激酶（GWD，EC 2.7.9.4）。

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