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1.发明申请VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF 审中-公开来自BACILLUS SUBTILIS的变体ALPHA-淀粉及其使用方法
公开(公告)号：WO2009149395A3
公开(公告)日：2010-03-25
申请号：PCT/US2009046480
申请日：2009-06-05
Applicant: DANISCO US INC GENENCOR DIV , CASCAO-PEREIRA LUIS , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , POWER SCOTT D , RAMER SANDRA W , TOPPOZADA AMR R , WALLACE LOUISE
Inventor： CASCAO-PEREIRA LUIS , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , POWER SCOTT D , RAMER SANDRA W , TOPPOZADA AMR R , WALLACE LOUISE
CPC classification number: C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
Abstract: Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α淀粉酶，其变体，编码它们的核酸和包含核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 这样的方法可能产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH，不存在钙的情况下和/或不存在葡糖淀粉酶的情况下使用。
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2.发明申请PRODUCTION OF GLUCOSE FROM STARCH USING ALPHA-AMYLASES FROM BACILLUS SUBTILIS 审中-公开使用来自BACILLUS SUBTILIS的ALPHA-AMYLASES从淀粉生产葡萄糖
公开(公告)号：WO2009149271A2
公开(公告)日：2009-12-10
申请号：PCT/US2009046279
申请日：2009-06-04
Applicant: DANISCO US INC , CASCAO-PEREIRA LUIS G , CHIN REGINA , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , PEPSIN MICHAEL J , POWER SCOTT D , RAMER SANDRA W , REQUADT CAROL A , SHAW ANDREW , TOPPOZADA AMR R , WALLACE LOUISE
Inventor： CASCAO-PEREIRA LUIS G , CHIN REGINA , CUEVAS WILLIAM A , ESTELL DAVID A , LEE SANG-KYU , PEPSIN MICHAEL J , POWER SCOTT D , RAMER SANDRA W , REQUADT CAROL A , SHAW ANDREW , TOPPOZADA AMR R , WALLACE LOUISE
Abstract: An alpha amylase from Bacillus subtilis (AmyE) produces significant amounts of glucose from various carbohydrate substrates, including vegetable starch, maltoheptaose, and maltotriose. Among other things, this advantageous property allows AmyE or variants thereof to be used in a saccharification reaction having a reduced or eliminated requirement for glucoamylase. The reduction or elimination of the glucoamylase requirement significantly improves the efficiency of the production of ethanol or high fructose corn syrup, for example.
Abstract translation: 来自枯草芽孢杆菌（AmyE）的α淀粉酶从各种碳水化合物底物产生显着量的葡萄糖，包括植物淀粉，麦芽庚糖和麦芽三糖。 除此之外，这种有利的性质允许AmyE或其变体用于具有葡糖淀粉酶降低或消除的糖化反应中。 葡糖淀粉酶需求的减少或消除显着提高了乙醇或高果糖玉米糖浆的生产效率。
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3.发明申请
公开(公告)号：WO2009100138A3
公开(公告)日：2009-12-10
申请号：PCT/US2009033087
申请日：2009-02-04
Applicant: DANISCO US INC GENENCOR DIV , PEPSIN MICHAEL J , RAMER SANDRA W , SHAW ANDREW
Inventor： PEPSIN MICHAEL J , RAMER SANDRA W , SHAW ANDREW
CPC classification number: C12N9/2417 , Y02E50/17
Abstract: Described are variants of Bacillus stearodhermophilus alpha-amylases with mutations at positions 181 or/and 182 having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.
Abstract translation: 描述了具有改变的淀粉水解曲线的位置181或/和182处具有突变的嗜热脂肪芽孢杆菌α-淀粉酶的变体。 这些变体具有改善的热稳定性和增加的比活性，导致在淀粉液化期间降低的峰值粘度和改变的最终粘度。 淀粉酶变体是有用的，例如在液化和其它淀粉降解过程中。
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公开(公告)号：WO2009149419A3
公开(公告)日：2009-12-10
申请号：PCT/US2009/046506
申请日：2009-06-05
Applicant: DANISCO US INC. , CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Inventor： CHIN, Regina , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , PEPSIN, Michael, J. , POWER, Scott, D. , RAMER, Sandra, W. , REQUADT, Carol, A. , SHAW, Andrew , TOPPOZADA, Amr, R. , WALLACE, Louise
Abstract: Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
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5.发明申请GEOBACILLUS STEAROTHERMOPHILUS ALPHA-AMYLASE (AMYS) VARIANTS WITH IMPROVED PROPERTIES 审中-公开具有改进性质的GEOBACILLUS STEAROTHERMOPHILUS ALPHA-AMYLASE（AMYS）变种
公开(公告)号：WO2009149130A2
公开(公告)日：2009-12-10
申请号：PCT/US2009/046034
申请日：2009-06-02
Applicant: DANISCO US INC. , CUEVAS, William, A. , ESTELL, David, A. , HADI, Sura, Hussain , LEE, Sang-Kyu , RAMER, Sandra, W. , SHAW, Andrew , TOPPOZADA, Amr, R. , WEYLER, Walter
Inventor： CUEVAS, William, A. , ESTELL, David, A. , HADI, Sura, Hussain , LEE, Sang-Kyu , RAMER, Sandra, W. , SHAW, Andrew , TOPPOZADA, Amr, R. , WEYLER, Walter
CPC classification number: D06M16/003 , C11D3/38609 , C11D3/38618 , C11D3/3869 , C12N9/2417 , C12Y302/01001 , D06L4/40
Abstract: Described are variants of a parent alpha-amylase that exhibits an alteration in at least one of the following properties relative to said parent alpha-amylase: specific activity, substrate specificity, substrate binding, substrate cleavage, thermal stability, pH-dependent activity, pH-dependent stability, oxidative stability, calcium dependency, pI, and wash performance. The variants are suitable for starch conversion, ethanol production, laundry washing, dish washing, hard surface cleaning, textile desizing, and/or sweetener production.
Abstract translation: 描述了相对于所述亲本α-淀粉酶在下列性质中表现出至少一种变化的亲本α-淀粉酶的变体：比活性，底物特异性，底物结合，底物切割，热稳定性，pH依赖性活性，pH 依赖性稳定性，氧化稳定性，钙依赖性，pI和洗涤性能。 这些变体适用于淀粉转化，乙醇生产，洗衣，洗碗，硬表面清洗，纺织品退浆和/或甜味剂生产。
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Patent Agency Ranking
公开(公告)号：WO2009061381A3
公开(公告)日：2009-11-19
申请号：PCT/US2008012413
申请日：2008-11-03
Applicant: DANISCO US INC GENENCOR DIV , SHAW ANDREW , RAMER SANDRA , POWER SCOTT D , SHETTY JAYARAMA K , PAULSON BRADLEY , SHARMA VIVEK , WARD DONALD , CASCAO-PEREIRA LUIS G , KELLIS JAMES T
Inventor： SHAW ANDREW , RAMER SANDRA , POWER SCOTT D , SHETTY JAYARAMA K , PAULSON BRADLEY , SHARMA VIVEK , WARD DONALD , CASCAO-PEREIRA LUIS G , KELLIS JAMES T
CPC classification number: C11D3/38681 , C11D3/386 , C12N9/2417 , D06M16/003
Abstract: Disclosed are compositions comprising variants of alpha-amylase that have alpha-amylase activity and which exhibit altered properties relative to a parent AmyS-like alpha-amylase from which they are derived. The compositions comprise an additional enzyme such as a phytase. Also disclosed are methods of using the compositions, and kits related thereto.
Abstract translation: 公开了包含α-淀粉酶变体的组合物，其具有α-淀粉酶活性，并且相对于其来源的亲本AmyS样α-淀粉酶表现出改变的性质。 组合物包含另外的酶如植酸酶。 还公开了使用组合物的方法和与其相关的试剂盒。
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7.发明申请VARIANT ALPHA-AMYLASES FROM BACILLUS SUBTILIS AND METHODS OF USE, THEREOF 审中-公开来自枯草芽孢杆菌的变体α-淀粉酶及其使用方法
公开(公告)号：WO2009149395A2
公开(公告)日：2009-12-10
申请号：PCT/US2009/046480
申请日：2009-06-05
Applicant: DANISCO US INC., GENENCOR DIVISION , CASCAO-PEREIRA, Luis , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , POWER, Scott, D. , RAMER, Sandra, W. , TOPPOZADA, Amr, R. , WALLACE, Louise
Inventor： CASCAO-PEREIRA, Luis , CUEVAS, William, A. , ESTELL, David, A. , LEE, Sang-Kyu , POWER, Scott, D. , RAMER, Sandra, W. , TOPPOZADA, Amr, R. , WALLACE, Louise
CPC classification number: C12N9/2417 , A21D8/042 , C12N9/2408 , C12P7/14 , C12P19/02 , C12P19/14 , G01N11/00 , Y02E50/17
Abstract: Alpha amylases from Bacillus subtilis (AmyE), variants thereof, nucleic acids encoding the same, and host cells comprising the nucleic acids are provided. Methods of using AmyE or variants thereof are disclosed, including liquefaction and/or saccharification of starch. Such methods may yield sugars useful for ethanol production or high fructose corn syrup production. In some cases, the amylases can be used at low pH, in the absence of calcium, and/or in the absence of a glucoamylase.
Abstract translation: 提供了来自枯草芽孢杆菌（AmyE）的α-淀粉酶，其变体，编码它的核酸和包含该核酸的宿主细胞。 公开了使用AmyE或其变体的方法，包括淀粉的液化和/或糖化。 此类方法可产生可用于乙醇生产或高果糖玉米糖浆生产的糖。 在一些情况下，淀粉酶可以在低pH下，在不存在钙的情况下和/或在不存在葡糖淀粉酶的情况下使用。
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8.发明申请
公开(公告)号：WO2009100138A2
公开(公告)日：2009-08-13
申请号：PCT/US2009/033087
申请日：2009-02-04
Applicant: DANISCO US INC., GENENCOR DIVISION , PEPSIN, Michael, J. , RAMER, Sandra, W. , SHAW, Andrew
Inventor： PEPSIN, Michael, J. , RAMER, Sandra, W. , SHAW, Andrew
CPC classification number: C12N9/2417 , Y02E50/17
Abstract: Described are variants of alpha (?)-amylases having altered starch hydrolysis profiles. The variants have improved thermostability and increased specific activity, resulting in reduced peak viscosity and altered final viscosity during starch liquefaction. The amylase variants are useful, e.g., in liquefaction and other starch degradation processes.
Abstract translation: 描述了具有改变的淀粉水解曲线的α（α） - 淀粉酶的变体。 这些变体具有改善的热稳定性和增加的比活性，导致在淀粉液化期间降低的峰值粘度和改变的最终粘度。 淀粉酶变体是有用的，例如在液化和其它淀粉降解过程中。
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公开(公告)号：WO2002029079A3
公开(公告)日：2002-04-11
申请号：PCT/US2001/031273
申请日：2001-10-04
Applicant: MAXYGEN, INC. , RAMER, Sandra, W. , HUISMAN, Gjalt , MILLIS, Jim , SHELDON, Roger , DEL CARDAYRE, Stephen , TOBIN, Matthew , COX, Anthony , DAVIS, S., Christopher
Inventor： RAMER, Sandra, W. , HUISMAN, Gjalt , MILLIS, Jim , SHELDON, Roger , DEL CARDAYRE, Stephen , TOBIN, Matthew , COX, Anthony , DAVIS, S., Christopher
IPC: C12P13/00
Abstract: Enantioselective or enantiospecific nitrilases and nitrile hydratases are used to produce R or S enantiomers of amides, and carboxylic acids. R-amino acids and S-amino acids are produced using such enantioselective enzymes. In addition, methods of producing and screening enantioselective nitrilases and nitrile hydratases are provided.
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公开(公告)号：WO0229079A2
公开(公告)日：2002-04-11
申请号：PCT/US0131273
申请日：2001-10-04
Applicant: MAXYGEN INC , RAMER SANDRA W , HUISMAN GJALT , MILLIS JIM , SHELDON ROGER , DEL CARDAYRE STEPHEN , TOBIN MATTHEW , COX ANTHONY , DAVIS S CHRISTOPHER
Inventor： RAMER SANDRA W , HUISMAN GJALT , MILLIS JIM , SHELDON ROGER , DEL CARDAYRE STEPHEN , TOBIN MATTHEW , COX ANTHONY , DAVIS S CHRISTOPHER
CPC classification number: C12P13/04 , C12N9/88 , C12P13/02 , C12P41/006
Abstract: Enantioselective or enantiospecific nitrilases and nitrile hydratases are used to produce R or S enantiomers of amides, and carboxylic acids. R-amino acids and S-amino acids are produced using such enantioselective enzymes. In addition, methods of producing and screening enantioselective nitrilases and nitrile hydratases are provided.
Abstract translation: 对映选择性或对映体特异性腈水解酶和腈水合酶用于产生酰胺和羧酸的R或S对映异构体。 使用这种对映选择性酶产生R-氨基酸和S-氨基酸。 另外，提供了生产和筛选对映选择性腈水解酶和腈水合酶的方法。
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