公开(公告)号：WO2018113431A1

公开(公告)日：2018-06-28

申请号：PCT/CN2017/109653

申请日：2017-11-07

Applicant: 广州格拉姆生物科技有限公司

Inventor： 张宏刚 ,  康小龙 ,  李莉

IPC: C12N15/81 ,  C12N1/19 ,  C12N15/66 ,  C12P19/12 ,  C12P19/04 ,  C12P19/00 ,  C12P21/02 ,  C12N15/56 ,  C12R1/865

CPC classification number: C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/81 ,  C12N2800/102 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P21/02 ,  C12Y302/01004 ,  C12Y302/01021

Abstract: 提供一种能降解纤维素生产益生纤维寡糖并分泌抗菌肽的多功能酿酒酵母，该重组酿酒酵母中含有能利用能降解纤维素生产益生纤维寡糖并分泌抗菌肽的酿酒酵母多基因共表达载体，该载体中含有降解纤维素的酶基因和抗菌肽基因。

公开(公告)号：WO2015181292A1

公开(公告)日：2015-12-03

申请号：PCT/EP2015/061837

申请日：2015-05-28

Applicant: NOVOZYMES A/S

Inventor： JENSEN, Kenneth ,  SCHNORR, Kirk Matthew ,  SALOMON, Jesper ,  HENRISSAT, Bernard

IPC: C12N9/88 ,  C11D3/386 ,  C12N9/24

CPC classification number: C11D3/38636 ,  C09K8/52 ,  C09K8/62 ,  C09K2208/24 ,  C11D3/38645 ,  C12N9/2402 ,  C12N9/2434 ,  C12N9/88 ,  C12Y302/0104 ,  C12Y402/02012 ,  E21B37/00 ,  E21B43/26

Abstract: The present invention relates to endoglucanases having xanthan degrading activity and polynucleotides encoding the endoglucanases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the endoglucanases.

Abstract translation: 本发明涉及具有黄原胶降解活性的内切葡聚糖酶和编码内切葡聚糖酶的多核苷酸。 本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及产生和使用内切葡聚糖酶的方法。

公开(公告)号：WO2014201595A1

公开(公告)日：2014-12-24

申请号：PCT/CN2013/001509

申请日：2013-12-06

Applicant: 中国科学院大连化学物理研究所

Inventor： 杨凌 ,  窦同意 ,  栾宏伟 ,  刘兴宝 ,  李世阳

IPC: C12N9/42 ,  C12N15/56 ,  C12N15/63 ,  C12N1/19 ,  C12N1/21 ,  C12P19/14 ,  C12R1/19 ,  C12R1/645

CPC classification number: C12P19/14 ,  C12N9/2434 ,  C12P19/02 ,  C12Y302/01037

Abstract: 提供了一种木糖苷酶Xyl_S及其编码基因与应用，该木糖苷酶的氨基酸序列如SEQ ID NO.2所示，其核苷酸编码序列如SEQ ID NO.1所示。该木糖苷酶具有β-木糖苷水解酶和β-葡萄糖苷酶双重活性，可特异性地水解相应的木糖苷键和葡萄糖苷键。

公开(公告)号：WO2014143734A2

公开(公告)日：2014-09-18

申请号：PCT/US2014027818

申请日：2014-03-14

Applicant: AMICUS THERAPEUTICS INC

Inventor： DO HUNG

IPC: A61K38/47

CPC classification number: A61K47/48215 ,  A61K38/30 ,  A61K38/47 ,  A61K47/60 ,  C07K14/65 ,  C07K2319/00 ,  C12N9/16 ,  C12N9/20 ,  C12N9/2402 ,  C12N9/2408 ,  C12N9/2434 ,  C12N9/2465 ,  C12N9/2488 ,  C12N9/96 ,  C12Y302/0102

Abstract: Disclosed herein are methods of chemical conjugation comprising contacting a lysosomal enzyme with a first crosslinking agent to introduce aldehyde groups; contacting a lysosomal targeting peptide with a second crosslinking agent to introduce a hydrazide group at the N-terminal residue; contacting the lysosomal enzyme with aldehyde groups of step a. with the lysosomal targeting peptide with a hydrazide group at the N-terminal residue of step b; and forming a lysosomal enzyme-lysosomal targeting peptide conjugate.

Abstract translation: 本文公开了化学共轭的方法，包括使溶酶体酶与第一交联剂接触以引入醛基; 使溶酶体靶向肽与第二交联剂接触以在N-末端残基引入酰肼基团; 使溶酶体酶与步骤a的醛基接触。 其中在步骤b的N末端残基处具有酰肼基团的溶酶体靶向肽; 并形成溶酶体酶 - 溶酶体靶向肽缀合物。

公开(公告)号：WO2014108900A1

公开(公告)日：2014-07-17

申请号：PCT/IL2014/050024

申请日：2014-01-09

Applicant: DESIGNER ENERGY LTD.

Inventor： MORAG, Ely ,  BARAK, Tal ,  KARPOL, Alon ,  ANBAR, Michael

IPC: C12N9/42 ,  C12P7/10 ,  C12P19/14

CPC classification number: C12N11/16 ,  C12N9/2434 ,  C12N9/2445 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01021 ,  Y02P20/582

Abstract: Compositions comprising unprocessed cell pellets of a cellulosome-producing microorganism grown on cellulosic biomass are provided. Further provided are methods for producing the compositions and uses thereof in hydrolysis of cellulosic substrates. In particular, the compositions advantageously contain extracellular beta-glucosidase, either expressed on the cells themselves or extrinsically added to the cell pellets.

Abstract translation: 提供了包含在纤维素生物质上生长的产生纤维素酶的微生物的未处理的细胞团的组合物。 进一步提供了用于生产组合物的方法及其在纤维素底物水解中的用途。 特别地，组合物有利地含有细胞外β-葡糖苷酶，其在细胞本身上表达或者外源性加入到细胞团中。

公开(公告)号：WO2014078546A3

公开(公告)日：2014-07-17

申请号：PCT/US2013070116

申请日：2013-11-14

Applicant: BP CORP NORTH AMERICA INC ,  LYON CHRISTOPHER SCOTT ,  LUGINBUHL PETER ,  STEGE JUSTIN TRENT

Inventor： LYON CHRISTOPHER SCOTT ,  LUGINBUHL PETER ,  STEGE JUSTIN TRENT

IPC: C12P19/14 ,  C12N9/42

CPC classification number: C12N9/2434 ,  C07K2319/00 ,  C12N9/2437 ,  C12P7/10 ,  C12P7/14 ,  C12P19/02 ,  C12P19/12 ,  C12P19/14 ,  C12P2203/00 ,  C12Y302/01091 ,  Y02E50/16

Abstract: The present disclosure relates to variant CBH II polypeptides that have improved specific activity, and compositions, e.g., cellulase compositions, comprising variant CBH II polypeptides. The variant CBH II polypeptides and related compositions can be used in variety of agricultural and industrial applications. The present disclosure further relates to nucleic acids encoding variant CBH II polypeptides and host cells that recombinantly express the variant CBH II polypeptides.

Abstract translation: 本公开涉及具有改善的比活性的变体CBH II多肽，以及包含变体CBH II多肽的组合物，例如纤维素酶组合物。 变体CBH II多肽和相关组合物可用于各种农业和工业应用。 本公开还涉及编码变体CBH II多肽的核酸和重组表达变体CBH II多肽的宿主细胞。

公开(公告)号：WO2013189878A1

公开(公告)日：2013-12-27

申请号：PCT/EP2013/062490

申请日：2013-06-17

Applicant: DSM IP ASSETS B.V.

Inventor： LOS, Alrik Pieter ,  HEIJNE, Wilbert Herman Marie ,  PEL, Herman Jan ,  DAMVELD, Robbertus Antonius ,  VONK, Brenda

IPC: C12N1/15 ,  C12N9/18 ,  C12N9/26 ,  C12N9/42 ,  C12N15/80 ,  C12P1/02 ,  C12P21/02

CPC classification number: C12N15/80 ,  C12N9/18 ,  C12N9/2408 ,  C12N9/2428 ,  C12N9/2434 ,  C12P1/02 ,  C12P21/02

Abstract: The present invention relates to isolated Rasamsonia promoter DNA sequences, to DNA constructs, vectors, and host cells comprising these promoters in operative association with coding sequences. The present invention also relates to methods for expressing a gene and/or producing a biological compound using the new promoters isolated. The present invention also relates to methods for altering the transcription level and/or regulation of an endogenous gene using the new promoter of the invention.

Abstract translation: 本发明涉及分离的Rasamsonia启动子DNA序列，涉及包含这些启动子的DNA构建体，载体和宿主细胞，其与编码序列有效结合。 本发明还涉及使用分离的新启动子来表达基因和/或生产生物化合物的方法。 本发明还涉及使用本发明的新启动子改变内源基因的转录水平和/或调节的方法。

公开(公告)号：WO2013185344A1

公开(公告)日：2013-12-19

申请号：PCT/CN2012/076993

申请日：2012-06-15

Applicant: JIANGSU UNIVERSITY ,  Cui, Fengjie

Inventor： Cui, Fengjie

IPC: C12P7/56 ,  C07C59/08 ,  C07C51/00 ,  C12R1/225

CPC classification number: C12P7/56 ,  C07C51/42 ,  C12M45/09 ,  C12M47/10 ,  C12M47/12 ,  C12N9/2434 ,  C12N9/2482 ,  C12P7/54 ,  C12P2203/00 ,  C07C59/08

Abstract: A method and system for producing lactic acid from biomass materials uses a mixed bacteria culture of at least one homofermentative lactic acid bacteria and at least one heterofermentative lactic acid bacteria in an integrated production system to increase the productivity and yield of lactic acid.

Abstract translation: 从生物质材料生产乳酸的方法和系统在综合生产系统中使用至少一种同型发酵乳酸菌和至少一种异质发酵乳酸菌的混合细菌培养物，以提高乳酸的生产率和产率。

公开(公告)号：WO2013163590A3

公开(公告)日：2013-12-19

申请号：PCT/US2013038477

申请日：2013-04-26

Applicant: NOVOZYMES INC ,  NOVOZYMES AS

Inventor： LIN JANINE ,  BOHAN DOREEN ,  MARANTA MICHELLE ,  BERESFORD LESLIE ,  LAMSA MICHAEL ,  SWEENEY MATT ,  WOGULIS MARK ,  ZNAMEROSKI ELIZABETH ,  RASMUSSEN FRANK WINTHER

IPC: C12N9/02 ,  A01H5/00 ,  C11D3/386 ,  C12N1/15 ,  C12N1/21 ,  C12N9/42 ,  D21C5/00

CPC classification number: C12N9/2437 ,  C11D3/38645 ,  C12N9/0071 ,  C12N9/2434 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01004 ,  D21C5/005

Abstract: The present invention relates to GH61 polypeptide variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Abstract translation: 本发明涉及GH61多肽变体。 本发明还涉及编码这些变体的多核苷酸; 核酸构建体，载体和包含多核苷酸的宿主细胞; 以及使用这些变体的方法。

公开(公告)号：WO2013182669A2

公开(公告)日：2013-12-12

申请号：PCT/EP2013/061767

申请日：2013-06-07

Applicant: DSM IP ASSETS B.V. ,  CONCORDIA UNIVERSITY

Inventor： TSANG, Adrian ,  POWLOWSKI, Justin ,  BUTLER, Gregory

IPC: C12N9/24

CPC classification number: C12N9/2405 ,  C12N9/0059 ,  C12N9/0071 ,  C12N9/2402 ,  C12N9/2434 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2477 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/88 ,  C12P7/10 ,  C12P19/02 ,  C12P19/14 ,  C12P2203/00 ,  C13K1/02 ,  C13K13/00 ,  C13K13/002 ,  Y02E50/16

Abstract: The present invention relates to a process for degrading biomass or pretreated biomass wherein an enzyme is used comprising a polypeptide having a. a polypeptide sequence as set forth in any one of SEQ ID Nos:3, 6, 9, 12, 15, 8, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 59, 61, 63, 65, 67, 69 and 71; b. a polypeptide that is at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to the any one of SEQ ID Nos:3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45, 48, 51, 54, 57, 59, 61, 63, 65, 67, 69 and 71; c.a polypeptide sequence encoded by nucleic acids sequence as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 56, 58,60, 62, 64, 66, 68 and 70, or nucleic acids that are at least 60%, preferably at least 70%, more preferably at least 80%, even more preferably at least 90%, 95%, 96%, 97%, 98% or 99% homologous to any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 56, 58, 60, 62, 64, 66, 68 and 70; d.a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the polynucleotide as set forth in any one of 2 SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 55, 56, 58, 60, 62, 64, 66, 68 and 70; or e.a polypeptide sequence encoded by a nucleic acids sequence hybridizing under stringent conditions to the reverse complement of a polynucleotide as set forth in any one of SEQ ID NOs: 1, 2, 4, 5, 7, 8, 10, 11, 13, 14, 16, 17, 19, 20, 22, 23, 25, 26, 28, 29, 31, 32, 34, 35, 37, 38, 40, 41, 43, 44, 46, 47, 49, 50, 52, 53, 56, 58, 60, 62, 64, 66, 68 and70.

Abstract translation: 本发明涉及降解生物质或预处理的生物质的方法，其中使用的酶包括具有a。 如SEQ ID No：3,6,9,12,158,21,24,27,30,33,36,39,42,45,48,51,54,54,45,45,48,45,45,45,45,45,45,45,45,45,45,45,45,45,55,55,52,52,58,54,54,54,54,54,54,54,54,54,54,54,54 57,59,61,63,65,67,69和71; 湾 与下列任何一种的至少60％，优选至少70％，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或99％同源的多肽 SEQ ID No：3,6,9。12，15， 67,69和71; 由SEQ ID NO：1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23中任一个所示的核酸序列编码的多肽序列 ，25,26,28,29,31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,56,58,60,62,64 ，66,68和70，或至少60％，优选至少70％，更优选至少80％，甚至更优选至少90％，95％，96％，97％，98％或 与SEQ ID NO：1,2,4,5,7,8,10,11,13,14,16,17,19,20,22,23,25,26,28,29中的任一个同源的99％ ，31,32,34,35,37,38,40,41,43,44,46,47,49,50,52,53,56,58,60,62,64,66,68和70; da多肽序列，其由在严格条件下与如SEQ ID NO：2,4,5,7,8,10,11,13,14,16， 17，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，44，46，47，49，50，52，53， 55，56，58，60，62，64，66，68及70条; 或由在严格条件下杂交的核酸序列编码的ea多肽序列与如SEQ ID NO：1,2,4,5,7,8,10,11,13， 14，16，17，19，20，22，23，25，26，28，29，31，32，34，35，37，38，40，41，43，44，46，47，49，50， 52,53,56,58,60,62,64,66,68和70。