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8 条记录 (耗时 0.017 秒)

    VIRAL POLYPEPTIDE FRAGMENTS THAT BIND CELLULAR POL II C-TERMINAL DOMAIN (CTD) AND THEIR USES
    1.
    发明申请
    VIRAL POLYPEPTIDE FRAGMENTS THAT BIND CELLULAR POL II C-TERMINAL DOMAIN (CTD) AND THEIR USES 审中-公开
    结合细胞pol II C-端结构域（CTD）的病毒多肽片段及其用途

    公开(公告)号：WO2018007615A1

    公开(公告)日：2018-01-11

    申请号：PCT/EP2017/067144

    申请日：2017-07-07

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY

    发明人： CUSACK, Stephen ,  PFLUG, Alexander ,  LUKARSKA, Maria

    IPC分类号： C12N9/12 ,  G06F19/16

    摘要： The present invention relates to in silico methods for identifying compounds which decrease or prevent the binding of the viral RNA-dependent RNA polymerase from the Orthomyxoviridae family or variant thereof, to its ligand, (preferably to cellular Pol II, more preferably to CTD), as well as methods of producing the identified compounds. The present invention also relates to a compounds identifiable and/or producible by said methods. The present invention also relates to antibodies directed against the binding site of the RNA-dependent RNA polymerase, to its ligand (in particular to cellular Pol II, in particular to CTD of Pol II) as well as nucleic acids encoding said antibodies and vectors comprising the nucleic acid. The present invention relates to a pharmaceutical composition producible according to said method, and/or comprising said compound, said antibody, said nucleic acid, or said vector. The present invention also relates to the use of said compound, said antibody, said nucleic acid, said vector or said pharmaceutical in treating, ameliorating, or preventing disease conditions caused by viral infections with viruses of the Orthomyxoviridae family.

    摘要翻译： 本发明涉及用于鉴定减少或防止病毒RNA依赖性RNA聚合酶从正粘病毒科或其变体与其变体结合的化合物的计算机方法， 配体（优选转化为细胞Pol II，更优选转化为CTD），以及生产所鉴定的化合物的方法。 本发明还涉及可通过所述方法鉴定和/或生产的化合物。 本发明还涉及针对所述RNA依赖性RNA聚合酶的结合位点的抗体，其配体（尤其是对细胞RNA聚合酶II，特别是RNA聚合酶II的CTD）以及核酸编码所述抗体和载体，其包括 核酸。 本发明涉及根据所述方法可产生的和/或包含所述化合物，所述抗体，所述核酸或所述载体的药物组合物。 本发明还涉及所述化合物，所述抗体，所述核酸，所述载体或所述药物在治疗，改善或预防由正粘病毒科病毒感染病毒引起的疾病中的用途。

    A NEW TYROSINE RECOMBINASE FOR GENETIC ENGINEERING
    2.
    发明申请
    A NEW TYROSINE RECOMBINASE FOR GENETIC ENGINEERING 审中-公开
    一种用于遗传工程的新型酪氨酸重组体

    公开(公告)号：WO2003004652A2

    公开(公告)日：2003-01-16

    申请号：PCT/EP2002/007176

    申请日：2002-06-28

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY ,  L'UNIVERSITE CATHOLIQUE DE LOUVAIN ,  STEWART, Francis, A. ,  ZHANG, Youming ,  HALLET, Bernard

    发明人： STEWART, Francis, A. ,  ZHANG, Youming ,  HALLET, Bernard

    IPC分类号： C12N15/52

    CPC分类号： C12N9/00 ,  C12N15/902

    摘要： The present invention is directed to methods and compositions for TnpI-mediated genetic engineering using the Bacillus thurungiensis recombinase TnpI and the TnpI recombination substrates TRT or TRT', and variants thereof. In particular, the invention relates to TRT or TRT' sequences, and variants thereof, vectors, cells and kits useful for TnpI-mediated genetic recombination, as well as methods for the use of TRT or TRT' sequences for TnpI-mediated genetic engineering.

    摘要翻译： 本发明涉及使用芽孢杆菌Thrungiensis重组酶TnpI和TnpI重组底物TRT或TRT'及其变体的TnpI介导的遗传工程的方法和组合物。 特别地，本发明涉及可用于TnpI介导的基因重组的TRT或TRT'序列及其变体，载体，细胞和试剂盒，以及用于TnpI介导的基因工程的TRT或TRT'序列的使用方法。

    A SYSTEM AND METHOD FOR AUTOMATIC PROTEIN SEQUENCING BY MASS SPECTROMETRY
    4.
    发明申请
    A SYSTEM AND METHOD FOR AUTOMATIC PROTEIN SEQUENCING BY MASS SPECTROMETRY 审中-公开
    一种通过质谱分析自动蛋白质测序的系统和方法

    公开(公告)号：WO2003046577A1

    公开(公告)日：2003-06-05

    申请号：PCT/EP2001/014041

    申请日：2001-11-30

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY ,  WILM, Matthias ,  NEUBAUER, Gitte, Jackie

    发明人： WILM, Matthias ,  NEUBAUER, Gitte, Jackie

    IPC分类号： G01N33/68

    CPC分类号： G01N33/6848 ,  G01N2458/15 ,  H01J49/04

    摘要： A method of deducing the sequence of a protein by analysing tandem mass spectrometry data. The protein is subjected to partial isotopic labelling by enzymatic digestion in a water mixture comprising a non-natural abundance of H 2 1s O. Differential scanning mass spectrometry is applied to the peptide fragments obtained from the digestion. Peaks in the spectra are analyzed to ascertain whether they arise from isotopically labeled fragments or not. A filtered spectrum is calculated that just comprises peaks from the y-ions. The sequence of the peptide is deduced by computing the difference in the mass between adjacent y-ion peaks.

    摘要翻译： 通过分析串联质谱数据推导蛋白质序列的方法。 蛋白质通过在包含非天然丰度的H2 <1s> O的水混合物中通过酶消化进行部分同位素标记。 差示扫描质谱法应用于从消化获得的肽片段。 分析光谱中的峰，以确定它们是否来自同位素标记的片段。 计算出仅包含来自y离子的峰的滤波光谱。 通过计算相邻y离子峰之间的质量差异来推导肽的序列。

    NUCLEIC ACID CONSTRUCT BINDING TO INFLUENZA POLYMERASE PB1 RNA SYNTHESIS ACTIVE SITE
    5.
    发明申请
    NUCLEIC ACID CONSTRUCT BINDING TO INFLUENZA POLYMERASE PB1 RNA SYNTHESIS ACTIVE SITE 审中-公开

    公开(公告)号：WO2020239822A1

    公开(公告)日：2020-12-03

    申请号：PCT/EP2020/064674

    申请日：2020-05-27

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY

    发明人： CUSACK, Stephen ,  DRNCOVA, Petra ,  KOUBA, Tomas

    IPC分类号： C12N15/11 ,  C12N9/12 ,  C07K14/005 ,  C30B29/58

    摘要： The present invention relates to a new nucleic acid construct capable of binding to the PB1 RNA synthesis active site of influenza polymerase. The nucleic acid construct allows capturing the structure of the transcription initiation state of influenza polymerase. The present invention further pertains to methods for obtaining images or crystallography data on an influenza polymerase in a functional or active state, methods for identifying, selecting or designing a compound which inhibits influenza RNA polymerase, and to such compounds per se. Further provided are pharmaceutical compositions comprising such compounds and the compounds or pharmaceutical compositions for use in treating, ameliorating, or preventing a disease caused by viral infections with negative-sense single stranded RNA viruses.

    DETECTION AND TREATMENT OF BREAST CANCER
    6.
    发明申请
    DETECTION AND TREATMENT OF BREAST CANCER 审中-公开
    乳腺癌的检测与治疗

    公开(公告)号：WO2013093489A2

    公开(公告)日：2013-06-27

    申请号：PCT/GB2012/053223

    申请日：2012-12-20

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY ,  UCL BUSINESS PLC ,  FAHY GURTEEN LABORATORIES LTD.

    发明人： ELLENBERG, Jan ,  NEUMANN, Beate ,  LODDO, Marco ,  WILLIAMS, Gareth ,  STOEBER, Kai

    IPC分类号： G01N33/574 ,  A61K38/17 ,  C07K14/47 ,  C12Q1/68

    CPC分类号： C12Q1/6886 ,  A61K38/1709 ,  A61K38/1754 ,  A61K38/30 ,  A61K45/06 ,  C07K14/4715 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158 ,  G01N33/57415 ,  A61K2300/00

    摘要： The present invention describes methods for determining the risk that a breast precursor lesion will progress to invasive breast cancer and/or the risk of recurrent non-invasive disease in a patient, comprising detecting the presence and/or level of PAPPA and/or PAPPA functional activity in a breast tissue sample obtained from the patient, wherein if PAPPA is not present, or is present at a reduced amount compared to a control, there is the risk of progression to invasive cancer and/or the risk or recurrent disease. In an alternative embodiment, the diagnosis can be carried out by identifying the proportion of mitotic cells in a patient sample that are in prophase or pro- metaphase, wherein if the proportion of cells in prophase or pro-metaphase is 30% or more,this indicates a risk of progression to invasive breast cancer and/or risk of recurrent disease. The present invention also enables the chemosensitisation of mitotically delayed breast cancer cells to anti-proliferative agents, preferably anti-mitotic agents, by restoring normal progression through mitosis. In this embodiment a first drug is applied to release breast cancer cells from the mitotic block and, sequentially,a second drug affecting proliferating cells is administered for cancer cell killing.

    摘要翻译： 本发明描述了用于确定乳腺前体病变将进入侵袭性乳腺癌的风险和/或患者中复发性非侵入性疾病的风险的方法，包括检测PAPPA和/或PAPPA功能的存在和/或水平 从患者获得的乳腺组织样品中的活性，其中如果PAPPA不存在或与对照相比以较低的量存在，则存在进展为侵袭性癌症和/或风险或复发性疾病的风险。 在另一个实施方案中，诊断可以通过鉴定在前期或前期中期的患者样品中有丝分裂细胞的比例来进行，其中如果前期或前期中期的细胞比例为30％以上，则 表示进展为浸润性乳腺癌和/或复发性疾病的风险。 本发明还通过恢复通过有丝分裂的正常进展，使得有丝分裂延迟的乳腺癌细胞对抗增殖剂（优选抗有丝分裂剂）的化学增敏作用。 在该实施方案中，施用第一种药物以从有丝分裂块释放乳腺癌细胞，并且依次施用影响增殖细胞的第二种药物用于癌细胞杀伤。

    MICROFLUIDIC SORTING DEVICES AND METHODS
    7.
    发明申请
    MICROFLUIDIC SORTING DEVICES AND METHODS 审中-公开
    微流体分配装置和方法

    公开(公告)号：WO2018054975A1

    公开(公告)日：2018-03-29

    申请号：PCT/EP2017/073775

    申请日：2017-09-20

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY

    发明人： MERTEN, Christoph A. ,  FRENZEL, Daniel

    IPC分类号： B01L3/00 ,  G01N15/10

    摘要： The present invention relates to the field of microfluidics and in particular to devices and methods for sorting objects in microfluidic channels. These devices and methods allow for fast and robust sorting in two-way and multi-way setups. They also enable sorting over extended periods of time.

    摘要翻译： 本发明涉及微流体领域，具体涉及用于在微流体通道中分选物体的装置和方法。 这些设备和方法允许在双向和多向设置中进行快速且稳健的分选。 他们还可以在较长时间内进行分类。

    ADENOVIRAL COAT PROTEIN DERIVED DELIVERY VEHICLES
    8.
    发明申请
    ADENOVIRAL COAT PROTEIN DERIVED DELIVERY VEHICLES 审中-公开
    腺病毒包膜蛋白衍生物递送车辆

    公开(公告)号：WO2017167988A1

    公开(公告)日：2017-10-05

    申请号：PCT/EP2017/057747

    申请日：2017-03-31

    申请人： THE EUROPEAN MOLECULAR BIOLOGY LABORATORY

    发明人： BERGER, Imre ,  GARZONI, Frédéric ,  FENDER, Pascal

    IPC分类号： C12N15/86 ,  C07K14/005 ,  C12N7/00 ,  A61K39/12

    摘要： The present invention relates to new adenoviral coat protein based delivery vehicles. They are based on a modified penton base protomers that assemble into VLPs. Exposed areas of the penton base proteins can be modified to allow the VLP to specifically bind to any target and/or to comprise any desired peptide epitope. Additional cargo, e.g. drugs, proteins, or nucleic acids, can reversibly or irreversibly attached to the VLP via engineered fibre protein fragments. The present invention relates to such engineered penton base protomers, engineered proteins comprising an adenovirus fibre protein N-terminal fragment specifically binding to an adenovirus fibre protein binding cleft of a penton base protomer, VLPs comprising the engineered penton base protomers and optionally engineered proteins comprising an adenovirus fibre protein N-terminal fragment specifically binding to an adenovirus fibre protein binding cleft of a penton base protomer, nucleic encoding the engineered proteins, the VLPs as well as methods of producing the proteins and VLPs.

    摘要翻译： 本发明涉及新的基于腺病毒外壳蛋白的递送载体。 它们基于装配成VLP的经修饰的五邻体基原体。 可以修饰五邻体碱基蛋白的暴露区域以允许VLP特异性结合任何靶标和/或包含任何期望的肽表位。 额外的货物，例如 药物，蛋白质或核酸可以通过工程化的纤维蛋白片段可逆地或不可逆地附着于VLP。 本发明涉及这样的工程化的五邻体碱基原体，包含特异性结合五邻体碱基前体的腺病毒纤维蛋白结合裂隙的腺病毒纤维蛋白N-末端片段的工程化蛋白，包含工程化的五邻体碱基前体的VLP和任选工程化的蛋白， 腺病毒纤维蛋白N-末端片段，其特异性结合五聚体碱基前体的腺病毒纤维蛋白结合裂隙，编码工程蛋白的核酸，VLP以及产生蛋白和VLP的方法。