公开(公告)号：WO2020006132A1

公开(公告)日：2020-01-02

申请号：PCT/US2019/039326

申请日：2019-06-26

Applicant: ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES ,  URNOV, Fyodor ,  STAMATOYANNOPOULOS, John A.

Inventor： URNOV, Fyodor ,  STAMATOYANNOPOULOS, John A.

IPC: C12Q1/68 ,  C12N9/22 ,  C07K14/195

Abstract: Provided herein are DNA binding domains comprising a plurality of repeat units, wherein each repeat unit is expanded or contracted in length. Also provided herein are DNA binding domains comprising a plurality of repeat units, wherein each repeat unit is separated from a neighboring repeat unit by a linker. In certain aspects, the linker includes a recognition site. Also disclosed are DNA binding proteins that include a fragment of N-cap sequence of a TALE protein. The TALE protein may be a Xanthomonas TALE protein.

公开(公告)号：WO2009042186A2

公开(公告)日：2009-04-02

申请号：PCT/US2008/011136

申请日：2008-09-25

Applicant: SANGAMO BIOSCIENCES, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA ,  AMACHER, Sharon ,  DOYON, Yannick ,  MCCAMMON, Jasmine ,  URNOV, Fyodor

Inventor： AMACHER, Sharon ,  DOYON, Yannick ,  MCCAMMON, Jasmine ,  URNOV, Fyodor

IPC: A01K67/027

CPC classification number: A01K67/0276 ,  A01K2217/075 ,  A01K2227/40 ,  A01K2267/03 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N2800/80

Abstract: Disclosed herein are methods and compositions for genomic editing of one or more genes in zebrafish, using fusion proteins comprising a zinc finger protein and a cleavage domain or cleavage half-domain. Polynucleotides encoding said fusion proteins are also provided, as are cells comprising said polynucleotides and fusion proteins.

Abstract translation: 本文公开了使用包含锌指蛋白和切割结构域或裂解半结构域的融合蛋白的斑马鱼中一种或多种基因的基因组编辑的方法和组合物。 还提供了编码所述融合蛋白的多核苷酸，以及包含所述多核苷酸和融合蛋白的细胞。

公开(公告)号：WO2009042164A1

公开(公告)日：2009-04-02

申请号：PCT/US2008/011089

申请日：2008-09-25

Applicant: DOW AGROSCIENCES LLC ,  SANGAMO BIOSCIENCES, INC. ,  GUPTA, Manju ,  PALTA, Asha, M. ,  NOVAK, Stephen ,  URNOV, Fyodor ,  GOPALAN, Sunita

Inventor： GUPTA, Manju ,  PALTA, Asha, M. ,  NOVAK, Stephen ,  URNOV, Fyodor ,  GOPALAN, Sunita

IPC: C12N15/82 ,  C12N9/22 ,  C12N15/90 ,  A01H5/00

CPC classification number: C12N15/8213 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/1092 ,  C12N15/8243 ,  C12N15/8274

Abstract: The present disclosure relates to engineered zinc finger proteins that target 5-enolpyruvyl shikimate-3-phosphate synthase (EPSPS) genes in plants and methods of using such zinc finger proteins in modulating gene expression, gene inactivation, and targeted gene modification. In particular, the disclosure pertains to zinc finger nucleases for targeted cleavage and alteration of EPSPS genes.

Abstract translation: 本公开涉及靶向植物中5-烯醇丙酮酸莽草酸-3-磷酸合酶（EPSPS）基因的工程化锌指蛋白，以及使用这种锌指蛋白调节基因表达，基因失活和靶向基因修饰的方法。 特别地，本公开涉及用于靶向切割和改变EPSPS基因的锌指核酸酶。

公开(公告)号：WO2009042163A3

公开(公告)日：2009-04-02

申请号：PCT/US2008/011087

申请日：2008-09-25

Applicant: SANGAMO BIOSCIENCES, INC. ,  DOYON, Yannick ,  URNOV, Fyodor

Inventor： DOYON, Yannick ,  URNOV, Fyodor

IPC: C12Q1/34 ,  G01N33/573

Abstract: Disclosed herein are methods and compositions for rapidly identifying and ranking nucleases for specific cleavage of a target sequence.

公开(公告)号：WO03027247A3

公开(公告)日：2003-05-22

申请号：PCT/US0230413

申请日：2002-09-24

Applicant: SANGAMO BIOSCIENCES INC ,  WOLFFE LIZ LF ,  MOORE MICHAEL ,  FARRIES TIMOTHY ,  COLLINGWOOD TREVOR ,  URNOV FYODOR

Inventor： WOLFFE ALAN P DI ,  MOORE MICHAEL ,  FARRIES TIMOTHY ,  COLLINGWOOD TREVOR ,  URNOV FYODOR

IPC: C12N5/0735 ,  G01N33/50 ,  A01N37/18 ,  A01N43/04 ,  A01N63/00 ,  C07H21/04 ,  C07K1/00 ,  C12N5/00 ,  C12N15/63

CPC classification number: C12N15/85 ,  A61K35/12 ,  C07H21/04 ,  C12N5/0606 ,  C12N15/62 ,  C12N15/63 ,  C12N2501/40 ,  C12N2503/02 ,  G01N33/5008 ,  G01N33/5023 ,  G01N33/5073

Abstract: Methods and compositions for modifying stem cells using one or more ZFPs are disclosed. Such methods and compositions are useful for facilitating processes such as, for example, dedifferentiating cells, differentiating stems cells into the desired phenotype, propagating stem cells and/or facilitating cloning.

Abstract translation: 公开了使用一种或多种ZFP修饰干细胞的方法和组合物。 此类方法和组合物可用于促进过程，例如去分化细胞，使干细胞分化成所需表型，增殖干细胞和/或促进克隆。

公开(公告)号：WO2020006131A2

公开(公告)日：2020-01-02

申请号：PCT/US2019/039325

申请日：2019-06-26

Applicant: ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES ,  STAMATOYANNOPOULOS, John A. ,  URNOV, Fyodor ,  PEARL, Jocelynn ,  THOMAS, Sean

Inventor： STAMATOYANNOPOULOS, John A. ,  URNOV, Fyodor ,  PEARL, Jocelynn ,  THOMAS, Sean

Abstract: DNA cleaving enzymes are disclosed. The DNA cleaving enzymes are fused to a heterologous DNA binding domain that is designed to bind to a target nucleic acid sequence. Nucleic acids and expression cassettes encoding the DNA cleaving enzymes are also provided. Methods for genome editing using the DNA cleaving enzymes, fusion proteins, and compositions thereof are disclosed herein.

公开(公告)号：WO2012012667A3

公开(公告)日：2012-01-26

申请号：PCT/US2011/044902

申请日：2011-07-21

Applicant: SANGAMO BIOSCIENCES, INC. ,  BOARD OF REGENTS, THE UNIVERSITY OF TEXAS SYSTEM ,  COLLINGWOOD, Trevor ,  COOPER, Laurence J.,N. ,  GREGORY, Philip D. ,  HOLMES, Michael C. ,  MILLER, Jeffrey C. ,  REBAR, Edward J. ,  REIK, Andreas ,  URNOV, Fyodor

Inventor： COLLINGWOOD, Trevor ,  COOPER, Laurence J.,N. ,  GREGORY, Philip D. ,  HOLMES, Michael C. ,  MILLER, Jeffrey C. ,  REBAR, Edward J. ,  REIK, Andreas ,  URNOV, Fyodor

IPC: C07K14/00

Abstract: Disclosed herein are methods and compositions for modulating the expression of a HLA locus or for selectively deleting or manipulating a HLA locus or HLA regulator.

公开(公告)号：WO2009131632A1

公开(公告)日：2009-10-29

申请号：PCT/US2009/002292

申请日：2009-04-13

Applicant: SANGAMO BIOSCIENCES, INC. ,  DEKELVER, Russell ,  HOLMES, Michael, C. ,  URNOV, Fyodor ,  GREGORY, Philip, D.

Inventor： DEKELVER, Russell ,  HOLMES, Michael, C. ,  URNOV, Fyodor ,  GREGORY, Philip, D.

IPC: C12N15/82 ,  C12N15/90

CPC classification number: C12N15/907 ,  C12N15/63 ,  C12N15/8213

Abstract: Disclosed herein are linear donor molecules comprising homology arms of 50-750 base pairs (e.g., 50-100 base pairs) flanking one or more sequences of interest. The donor molecules and/or compositions comprising these molecules can be used in methods for targeted integration of an exogenous sequence into a specified region of interest in the genome of a cell.

Abstract translation: 本文公开了包含侧翼于一个或多个感兴趣序列的50-750个碱基对（例如，50-100个碱基对）的同源臂的线性供体分子。 包含这些分子的供体分子和/或组合物可用于将外源序列靶向整合到细胞基因组中的特定感兴趣区域的方法中。

公开(公告)号：WO2008133938A3

公开(公告)日：2009-04-02

申请号：PCT/US2008005282

申请日：2008-04-24

Applicant: SANGAMO BIOSCIENCES INC ,  DEKELVER RUSSELL ,  GREGORY PHILIP D ,  PASCHON DAVID ,  URNOV FYODOR ,  TAM PHILLIP

Inventor： DEKELVER RUSSELL ,  GREGORY PHILIP D ,  PASCHON DAVID ,  URNOV FYODOR ,  TAM PHILLIP

IPC: C12N15/10 ,  C12N15/90

CPC classification number: C12N15/907 ,  C07K14/435 ,  C07K14/43595 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/1082 ,  C12N15/113 ,  C12N2800/10 ,  C12N2800/30 ,  C12Y301/21004

Abstract: Disclosed herein are methods and compositions for targeted integration of an exogenous sequence into the human PPP1R12C locus, for example, for expression of a polypeptide of interest.

Abstract translation: 本文公开了用于将外源序列靶向整合到人PPP1R12C基因座中的方法和组合物，例如用于表达感兴趣的多肽。

公开(公告)号：WO2007014275A2

公开(公告)日：2007-02-01

申请号：PCT/US2006/029027

申请日：2006-07-26

Applicant: SANGAMO BIOSCIENCES, INC. ,  HOLMES, Michael C. ,  URNOV, Fyodor ,  GREGORY, Philip D. ,  REBAR, Edward J. ,  BRENNAN, Sean M.

Inventor： HOLMES, Michael C. ,  URNOV, Fyodor ,  GREGORY, Philip D. ,  REBAR, Edward J. ,  BRENNAN, Sean M.

IPC: C12N15/09

CPC classification number: C12N15/907 ,  A61K48/0008 ,  A61K48/005 ,  A61K48/0058 ,  C07K2319/00 ,  C07K2319/81 ,  C12N9/22 ,  C12N15/62 ,  C12N15/90

Abstract: Disclosed herein are methods and compositions for targeted integration of a exogenous sequence into a predetermined target site in a genome for use, for example, in protein expression and gene inactivation.

Abstract translation: 本文公开了用于将外源序列靶向整合到基因组中的预定靶位点以用于例如蛋白质表达和基因失活的方法和组合物。