公开(公告)号：WO2011150411A1

公开(公告)日：2011-12-01

申请号：PCT/US2011/038464

申请日：2011-05-27

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey L ,  RAKITSKY, Walt

发明人： FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey L ,  RAKITSKY, Walt

IPC分类号： A23D9/00 ,  C11B5/00

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of food compositions, oils, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing food compositions, and transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产食品组合物，油，燃料，油脂化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造食品组合物，以及运输燃料如可再生柴油，生物柴油和可再生喷气燃料， 以及诸如功能流体，表面活性剂，肥皂和润滑剂的油脂化学品。

公开(公告)号：WO2010036774A1

公开(公告)日：2010-04-01

申请号：PCT/US2009/058174

申请日：2009-09-24

申请人： MEDIMMUNE, LLC ,  THOMPSON, Mark ,  WEE, Janice ,  NAGPAL, Akanksha

发明人： THOMPSON, Mark ,  WEE, Janice ,  NAGPAL, Akanksha

IPC分类号： A61K39/155 ,  C12N7/02

CPC分类号： C12N7/00 ,  A61K2039/5254 ,  C12N2760/16051 ,  C12N2760/18551

摘要： The present invention provides methods for the purification of cell-associated viruses from adherent cells (e.g., MDCK or Vero cells). In particular, the present invention provides purification methods for the production of immunogenic compositions comprising a live attenuated cell-associated virus (e.g., an attenuated respiratory syncytial virus (RSV) or cold- adapted, and/or temperature sensitive influenza virus) that result in levels of host cell DNA (HCD), host cell protein (HCP) and non-specific endonuclease (e.g., Benzonase), which are below the specifications required by regulatory agencies. The immunogenic compositions can be used to actively immunize subjects or to generate antibodies for a variety of uses, including passive immunization and diagnostic immunoassays.

摘要翻译： 本发明提供了用于从贴壁细胞（例如MDCK或Vero细胞）纯化细胞相关病毒的方法。 特别地，本发明提供用于产生免疫原性组合物的纯化方法，其包含活减毒细胞相关病毒（例如，减毒呼吸道合胞病毒（RSV）或冷适应性和/或温度敏感型流感病毒），其导致 宿主细胞DNA（HCD），宿主细胞蛋白（HCP）和非特异性内切核酸酶（例如Benzonase）的水平低于管理机构要求的规格。 免疫原性组合物可用于主动免疫受试者或产生用于多种用途的抗体，包括被动免疫和诊断性免疫测定。

公开(公告)号：WO2011130573A1

公开(公告)日：2011-10-20

申请号：PCT/US2011/032582

申请日：2011-04-14

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  DECKER, Stephen, M. ,  WEE, Janice

发明人： FRANKLIN, Scott ,  DECKER, Stephen, M. ,  WEE, Janice

IPC分类号： C12N1/00 ,  A61K31/20 ,  A23D9/00

CPC分类号： C12P7/6463 ,  C10G3/40 ,  C10G3/52 ,  C10G2300/1014 ,  C10L1/026 ,  C10L1/08 ,  C10L1/19 ,  C10L10/08 ,  C10L2200/043 ,  C10L2200/0469 ,  C10L2200/0476 ,  C10L2200/0492 ,  C10L2290/26 ,  C10L2290/54 ,  C10L2290/544 ,  C11B1/06 ,  C11B1/10 ,  C11C1/025 ,  C11C3/00 ,  C11C3/003 ,  C11C3/10 ,  C11C3/12 ,  C12P7/649 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Oleaginous yeast can be used to produce oil that can be extracted from oleaginous yeast biomass and converted into a wide variety of useful products, including fuels, hydrocarbon compositions, and/or oleochemicals.

摘要翻译： 油性酵母可用于生产可从含油酵母生物质提取的油，并转化成各种有用的产品，包括燃料，烃组合物和/或油脂化学品。

公开(公告)号：WO2012061647A2

公开(公告)日：2012-05-10

申请号：PCT/US2011/059224

申请日：2011-11-03

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  LU, Wenhua ,  RAKITSKY, Walter ,  RODRIGUEZ, Felipe Arana ,  RUDENKO, George ,  WEE, Janice ,  ZHAO, Xinhua

发明人： FRANKLIN, Scott ,  LU, Wenhua ,  RAKITSKY, Walter ,  RODRIGUEZ, Felipe Arana ,  RUDENKO, George ,  WEE, Janice ,  ZHAO, Xinhua

IPC分类号： C11B1/02 ,  C12P7/64 ,  H01B3/22 ,  H01F27/12

CPC分类号： C12P7/6463 ,  A23D9/007 ,  A23D9/02 ,  C10M169/041 ,  C11B1/025 ,  C11B1/06 ,  C11B1/10 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  C12N9/2442 ,  C12N15/79 ,  C12P7/6409 ,  C12Y301/02014 ,  H01B3/20 ,  H01F27/105 ,  H01F27/321

摘要： Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

摘要翻译： 提供了用于从由微生物产生的脂质生产介电流体的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 含有编码例如蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，脂质途径修饰酶，脂肪酰基-ACP硫酯酶，去饱和酶，脂肪酰辅酶A /醛的外源基因的微藻细胞 还原酶和/或酰基载体蛋白质可用于制造介电流体。

公开(公告)号：WO2011150410A2

公开(公告)日：2011-12-01

申请号：PCT/US2011/038463

申请日：2011-05-27

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey L ,  RAKITSKY, Walt

发明人： FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey L ,  RAKITSKY, Walt

IPC分类号： A23D9/02 ,  A23D7/02

CPC分类号： C12N15/79 ,  A23D7/00 ,  A23D9/00 ,  A23K20/158 ,  C11B1/00 ,  C11C3/00 ,  C12N9/0071 ,  C12N9/16 ,  C12N9/2431 ,  C12N9/2465 ,  C12N15/67 ,  C12N15/8216 ,  C12P7/64 ,  C12P7/6445 ,  C12P7/6463 ,  C12Y114/19001 ,  C12Y301/02 ,  C12Y301/02014 ,  C12Y302/01022 ,  C12Y302/01026 ,  Y02E50/13 ,  Y02P30/20 ,  Y02T50/678

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪醛脱羰酶和/或酰基载体蛋白质可用于制造运输燃料如可再生柴油，生物柴油和可再生喷气燃料，以及油脂化学品 例如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：WO2011130576A1

公开(公告)日：2011-10-20

申请号：PCT/US2011/032586

申请日：2011-04-14

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  PIECHOCKI, John ,  NORRIS, Leslie, M. ,  DECKER, Stephen, M. ,  WEE, Janice ,  ZDANIS, Dana

发明人： FRANKLIN, Scott ,  PIECHOCKI, John ,  NORRIS, Leslie, M. ,  DECKER, Stephen, M. ,  WEE, Janice ,  ZDANIS, Dana

IPC分类号： A23C9/12

CPC分类号： A21D2/267 ,  A23L7/198 ,  A23L33/115 ,  A23L33/14 ,  A23L33/145 ,  A23V2002/00 ,  A23V2200/254 ,  A23V2250/76

摘要： The invention provides novel oleaginous yeast biomass, yeast oil, food compositions comprising oleaginous yeast biomass, whole oleaginous yeast cells, and/or yeast oil in combination with one or more edible ingredients, and methods of making such compositions by combining oleaginous yeast biomass or yeast oil with other edible ingredients.

摘要翻译： 本发明提供新颖的含油酵母生物质，酵母油，包含含油酵母生物质，整个含油酵母细胞和/或酵母油与一种或多种可食用成分组合的食品组合物，以及通过将含油酵母生物质或酵母 油与其他可食用成分。

公开(公告)号：WO2008094438A1

公开(公告)日：2008-08-07

申请号：PCT/US2008/000918

申请日：2008-01-23

申请人： KOSAN BIOSCIENCES INCORPORATED ,  ASHLEY, Gary ,  MENZELLA, Hugo, G. ,  WEE, Janice, Lau ,  CARNEY, John, R.

发明人： ASHLEY, Gary ,  MENZELLA, Hugo, G. ,  WEE, Janice, Lau ,  CARNEY, John, R.

IPC分类号： C12P19/62 ,  A01N43/04 ,  A61K31/70

CPC分类号： C12P17/10 ,  C07D225/06

摘要： Macrolactams are made by feeding aromatic amino acids as replacement starter units to a mutant strain of the geldanamycin-producing microorganism Streptomyces hygroscopicus var. geldanus NRRL 3602, wherein the gene cluster encoding enzymes for the biosynthesis of the natural starter unit 3-amino-5-hydroxybenzoic acid has been deleted.

摘要翻译： 通过将芳香族氨基酸作为替代起始单位加入到产生格尔德霉素的微生物链霉菌吸湿链霉菌变异体的突变菌株中来制备麦芽糖内酰胺。 格尔氏菌NRRL 3602，其中编码天然起始单元3-氨基-5-羟基苯甲酸生物合成酶的基因簇已被缺失。

公开(公告)号：WO2012106560A9

公开(公告)日：2012-08-09

申请号：PCT/US2012/023696

申请日：2012-02-02

申请人： SOLAZYME, INC. ,  FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey ,  RAKITSKY, Walt ,  ZHAO, Xinhua ,  BHAT, Riyaz

发明人： FRANKLIN, Scott ,  SOMANCHI, Aravind ,  WEE, Janice ,  RUDENKO, George ,  MOSELEY, Jeffrey ,  RAKITSKY, Walt ,  ZHAO, Xinhua ,  BHAT, Riyaz

IPC分类号： C12P7/64 ,  C12N1/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

公开(公告)号：WO2012106560A1

公开(公告)日：2012-08-09

申请号：PCT/US2012023696

申请日：2012-02-02

申请人： SOLAZYME INC ,  FRANKLIN SCOTT ,  SOMANCHI ARAVIND ,  WEE JANICE ,  RUDENKO GEORGE ,  MOSELEY JEFFREY ,  RAKITSKY WALT ,  ZHAO XINHUA ,  BHAT RIYAZ

发明人： FRANKLIN SCOTT ,  SOMANCHI ARAVIND ,  WEE JANICE ,  RUDENKO GEORGE ,  MOSELEY JEFFREY ,  RAKITSKY WALT ,  ZHAO XINHUA ,  BHAT RIYAZ

IPC分类号： C12P7/64 ,  C12N1/13

CPC分类号： C12P7/6463 ,  C07C69/52 ,  C10L1/02 ,  C10L2200/0476 ,  C10L2290/26 ,  C10L2290/30 ,  C12N9/0071 ,  C12N9/1029 ,  C12N9/16 ,  C12N15/52 ,  Y02E50/13

摘要： Methods and compositions for the production of oil, fuels, oleochemicals, and other compounds in recombinant microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a lipase, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a keto acyl-ACP synthase enzyme, a fatty acyl-ACP thioesterase, a fatty acyl-CoA/aldehyde reductase, a fatty acyl-CoA reductase, a fatty aldehyde reductase, a fatty acid hydroxylase, a desaturase enzyme, a fatty aldehyde decarbonylase, and/or an acyl carrier protein are useful in manufacturing transportation fuels such as renewable diesel, biodiesel, and renewable jet fuel, as well as oleochemicals such as functional fluids, surfactants, soaps and lubricants.

摘要翻译： 提供了用于在重组微生物中生产油，燃料，油化合物和其它化合物的方法和组合物，包括含油微生物和这种微生物的低成本培养的方法。 包含编码例如脂肪酶，蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，酮酰基-ACP合成酶，脂肪酰基-ACP硫酯酶，脂肪酰辅酶A的外源基因的微藻细胞 /醛还原酶，脂肪酰辅酶A还原酶，脂肪醛还原酶，脂肪酸羟化酶，去饱和酶，脂肪醛脱羰酶和/或酰基载体蛋白，可用于制造运输燃料如可再生柴油，生物柴油， 和可再生喷气燃料，以及油化学品如功能流体，表面活性剂，肥皂和润滑剂。

公开(公告)号：WO2012061647A3

公开(公告)日：2012-08-09

申请号：PCT/US2011059224

申请日：2011-11-03

申请人： SOLAZYME INC ,  FRANKLIN SCOTT ,  LU WENHUA ,  RAKITSKY WALTER ,  RODRIGUEZ FELIPE ARANA ,  RUDENKO GEORGE ,  WEE JANICE ,  ZHAO XINHUA

发明人： FRANKLIN SCOTT ,  LU WENHUA ,  RAKITSKY WALTER ,  RODRIGUEZ FELIPE ARANA ,  RUDENKO GEORGE ,  WEE JANICE ,  ZHAO XINHUA

IPC分类号： C11B1/02 ,  C12P7/64 ,  H01B3/22 ,  H01F27/12

CPC分类号： C12P7/6463 ,  A23D9/007 ,  A23D9/02 ,  C10M169/041 ,  C11B1/025 ,  C11B1/06 ,  C11B1/10 ,  C11B3/001 ,  C11B3/006 ,  C11B3/008 ,  C11B3/06 ,  C11B3/10 ,  C11B3/14 ,  C12N9/2442 ,  C12N15/79 ,  C12P7/6409 ,  C12Y301/02014 ,  H01B3/20 ,  H01F27/105 ,  H01F27/321

摘要： Methods and compositions for the production of dielectric fluids from lipids produced by microorganisms are provided, including oil-bearing microorganisms and methods of low cost cultivation of such microorganisms. Microalgal cells containing exogenous genes encoding, for example, a sucrose transporter, a sucrose invertase, a fructokinase, a polysaccharide-degrading enzyme, a lipid pathway modification enzyme, a fatty acyl-ACP thioesterase, a desaturase, a fatty acyl-CoA/aldehyde reductase, and/or an acyl carrier protein are useful in manufacturing dielectric fluids.

摘要翻译： 提供了由微生物产生的脂质制备介电流体的方法和组合物，包括含油微生物和低成本培养这些微生物的方法。 包含编码例如蔗糖转运蛋白，蔗糖转化酶，果糖激酶，多糖降解酶，脂质途径修饰酶，脂肪酰基-ACP硫酯酶，去饱和酶，脂肪酰基-CoA /醛的外源基因的微藻细胞 还原酶和/或酰基载体蛋白质可用于制造介电流体。