公开(公告)号：WO2005032496A3

公开(公告)日：2005-04-14

申请号：PCT/US2004/007095

申请日：2004-03-08

Applicant: DIVERSA CORPORATION ,  BORNSCHEUER, Uwe, T. ,  WEINER, David, Paul ,  HITCHMAN, Tim ,  LYON, Jonathan ,  WONGSAKUL, Sirirung

Inventor： BORNSCHEUER, Uwe, T. ,  WEINER, David, Paul ,  HITCHMAN, Tim ,  LYON, Jonathan ,  WONGSAKUL, Sirirung

IPC: C12Q1/34 ,  C12N9/18 ,  C07H21/04

Abstract: The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphlipase A, B, C and D acitvity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzyme, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

公开(公告)号：WO2005032496A2

公开(公告)日：2005-04-14

申请号：PCT/US2004007095

申请日：2004-03-08

Applicant: DIVERSA CORP ,  BORNSCHEUER UWE T ,  WEINER DAVID PAUL ,  HITCHMAN TIM ,  LYON JONATHAN ,  WONGSAKUL SIRIRUNG

Inventor： BORNSCHEUER UWE T ,  WEINER DAVID PAUL ,  HITCHMAN TIM ,  LYON JONATHAN ,  WONGSAKUL SIRIRUNG

IPC: A01H5/00 ,  A01H5/10 ,  A01K67/027 ,  A23C7/00 ,  A23C9/20 ,  A23C19/05 ,  A23C19/06 ,  A23D7/00 ,  A23L1/00 ,  A61K20060101 ,  A61K6/00 ,  A61K8/37 ,  A61K8/60 ,  A61K8/66 ,  A61K31/7088 ,  A61K31/7105 ,  A61K38/00 ,  A61K38/46 ,  A61Q5/06 ,  A61Q11/00 ,  A61Q19/00 ,  C07H21/00 ,  C07H21/02 ,  C07K16/40 ,  C07K19/00 ,  C10M129/70 ,  C10M129/76 ,  C10M177/00 ,  C11D3/386 ,  C12C1/047 ,  C12C5/00 ,  C12C11/00 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12N9/20 ,  C12N11/00 ,  C12N15/10 ,  C12N15/55 ,  C12N15/82 ,  C12P7/52 ,  C12P7/62 ,  C12P7/64 ,  C12P19/34 ,  C12P21/08 ,  C12Q1/02 ,  C12Q1/34 ,  C12Q1/68 ,  D06M15/00 ,  D06M16/00 ,  G01N33/573 ,  A61K

CPC classification number: C12N9/20 ,  A23C9/20 ,  A23C19/05 ,  A23C19/063 ,  A23K10/14 ,  A23K20/189 ,  A23L29/06 ,  A23V2002/00 ,  A61K8/0216 ,  A61K8/375 ,  A61K8/606 ,  A61K8/66 ,  A61K38/465 ,  A61K2800/86 ,  A61Q5/06 ,  A61Q11/00 ,  A61Q19/00 ,  C10M129/70 ,  C10M129/76 ,  C10M2207/281 ,  C10M2207/289 ,  C10M2207/40 ,  C11C3/08 ,  C11D3/386 ,  C11D3/38627 ,  C11D3/38636 ,  C12C1/047 ,  C12C5/004 ,  C12C11/003 ,  C12N9/14 ,  C12N9/16 ,  C12N9/18 ,  C12P7/6418 ,  C12Q1/34 ,  C12Y301/01003 ,  D06M16/003 ,  D06M2200/12 ,  D06M2200/30 ,  D06M2200/50 ,  G01N33/573 ,  G01N2500/02

Abstract: The invention provides hydrolases, polynucleotides encoding them, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides, e.g., enzymes, having a hydrolase activity, e.g., an esterase, acylase, lipase, phospholipase (e.g., phosphlipase A, B, C and D acitvity, patatin activity, lipid acyl hydrolase (LAH) activity) or protease activity, including thermostable and thermotolerant hydrolase activity, and polynucleotides encoding these enzyme, and making and using these polynucleotides and polypeptides. The hydrolase activities of the polypeptides and peptides of the invention include esterase activity, lipase activity (hydrolysis of lipids), acidolysis reactions (to replace an esterified fatty acid with a free fatty acid), transesterification reactions (exchange of fatty acids between triglycerides), ester synthesis, ester interchange reactions, phospholipase activity and protease activity (hydrolysis of peptide bonds). The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts, including the manufacture of cosmetics and nutraceuticals. In another aspect, the polypeptides of the invention are used to synthesize enantiomerically pure chiral products.

Abstract translation: 本发明提供水解酶，编码它们的多核苷酸，以及制备和使用这些多核苷酸和多肽的方法。 在一个方面，本发明涉及具有水解酶活性的多肽，例如酯酶，酰基转移酶，脂肪酶，磷脂酶（例如，磷脂酶A，B，C和D活性，patatin活性，脂质酰基水解酶（ LAH）活性）或蛋白酶活性，包括热稳定和耐热水解酶活性，以及​​编码这些酶的多核苷酸，以及制备和使用这些多核苷酸和多肽。 本发明的多肽和肽的水解酶活性包括酯酶活性，脂肪酶活性（脂质水解），酸解反应（用游离脂肪酸代替酯化脂肪酸），酯交换反应（甘油三酸酯之间的脂肪酸交换）， 酯合成，酯交换反应，磷脂酶活性和蛋白酶活性（肽键的水解）。 本发明的多肽可用于各种药物，农业和工业环境中，包括制造化妆品和营养品。 另一方面，本发明的多肽用于合成对映体纯的手性产物。