公开(公告)号：WO2015092575A1

公开(公告)日：2015-06-25

申请号：PCT/IB2014/066284

申请日：2014-11-24

Applicant: BASF SE ,  BASF (CHINA) COMPANY LIMITED

Inventor： PETZKE, Lutz ,  HEROLD, Andrea ,  FLECK, Christian ,  TREIER-MARXEN, Katrin ,  ÖDMAN, Peter ,  DICKHAUT, Joachim ,  WEINGARTEN, Melanie ,  LUZHETSKY, Andriy ,  REBETS, Yuriy ,  BRÖTZ, Elke ,  MANDERSCHEID, Niko ,  MYRONOVSKYI, Maksym

IPC: C12N1/21 ,  C12N15/10 ,  C12N15/31 ,  C12N15/76 ,  C12P1/06 ,  C12Q1/04 ,  C12R1/47

CPC classification number: C12N15/52 ,  C07K14/36 ,  C12P17/181

Abstract: Provided are polynucleotides and polypeptides involved in the biosynthesis of pamamycin and pamamycin precursors as well as vectors and recombinant microorganisms comprising the polynucleotides and polypeptides. Also provided are methods for the production of pamamycins, in particular pamamycin 607 and pamamycin 621, and their precursors.

Abstract translation: 提供参与帕米霉素和帕米霉素前体的生物合成以及包含多核苷酸和多肽的载体和重组微生物的多核苷酸和多肽。 还提供了生产帕拉霉素，特别是帕米霉素607和帕米霉素621及其前体的方法。

公开(公告)号：WO2020249546A1

公开(公告)日：2020-12-17

申请号：PCT/EP2020/065929

申请日：2020-06-09

Applicant: BASF SE

Inventor： TREIER-MARXEN, Katrin ,  SCHOOF, Sebastian ,  SCHAEDLER, Andreas ,  FREYER, Stephan ,  KOPF, Michael Helmut

IPC: C07K1/14 ,  C11D3/386 ,  C12N9/00

Abstract: The present invention relates to a method for recovering a protein of interest from a fermentation broth by adding a salt of a divalent cation and increasing the pH.

公开(公告)号：WO2017097869A1

公开(公告)日：2017-06-15

申请号：PCT/EP2016/080165

申请日：2016-12-08

Applicant: BASF SE

Inventor： TREIER-MARXEN, Katrin ,  DECKERT, Petra ,  SCHAEDLER, Andreas ,  NAVICKAS, Vaidotas ,  WENGERT, Volker ,  KAEDING, Thomas

IPC: B01D61/16 ,  C12N1/02 ,  C07K1/14

CPC classification number: C12N1/02 ,  B01D61/147 ,  B01D61/16 ,  B01D2311/04 ,  B01D2311/18 ,  B01D2315/16 ,  C07K1/145 ,  C12P21/02

Abstract: The present invention is directed to a method of purifying a protein of interest from the particulate matter of a fermentation broth comprising the step of purifying the protein of interest from the particulate matter of the fermentation broth, wherein the step of purifying the protein of interest from the particulate matter of the fermentation broth comprises one or more washing steps, which comprises contacting the particulate matter with a washing solution comprising one or more conditions that favor the solubilization of the protein of interest and/or the desorption of the protein of interest from the particulate matter.

Abstract translation: 本发明涉及从发酵液微粒物质中纯化感兴趣的蛋白质的方法，其包括从发酵液微粒物质中纯化感兴趣的蛋白质的步骤，其中所述 从发酵液的颗粒物质中纯化感兴趣的蛋白质的步骤包括一个或多个洗涤步骤，该步骤包括使颗粒物质与洗涤溶液接触，所述洗涤溶液包含一个或多个有利于感兴趣的蛋白质和/或 目标蛋白质从颗粒物质中解吸。