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    COMPOSITIONS AND METHODS FOR DETECTION OF GENETIC DEAFNESS GENE MUTATION
    1.
    发明申请
    COMPOSITIONS AND METHODS FOR DETECTION OF GENETIC DEAFNESS GENE MUTATION 审中-公开
    用于检测遗传多样性基因突变的组合物和方法

    公开(公告)号：WO2017008177A1

    公开(公告)日：2017-01-19

    申请号：PCT/CN2015/000505

    申请日：2015-07-14

    Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

    Inventor： XIANG, Guangxin ,  LIU, Xuezhong ,  CHAI, Xingping ,  XING, Wanli ,  CHENG, Jing

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6883 ,  C12Q1/6827 ,  C12Q2600/156 ,  C12Q2600/16 ,  C12Q2563/103 ,  C12Q2563/149 ,  C12Q2565/519

    Abstract: In one aspect, a kit for detection of genetic gene mutation is provided, which is used for detecting nine deafness gene mutations of Caucasian populations, including GJB2 (c. 35delG, c. 167delT, c. 132G>C, and c. 269T>C), GJB6 (c. del309kb), SLC26A4 (c. 707T>C and c. 1246A>C), 12S rRNA (m. 1555A>G and m. 7444G>A). In another aspect, a method is provided, which method comprises labeling a target molecule with a luminophore, coupling the target molecule to a particle, and binding to a probe molecule on microarray. In some aspects, this technology, with high sensitivity, enables the detection and interpretation of molecular interactions in an efficient way.

    Abstract translation: 在一方面，提供了用于检测遗传基因突变的试剂盒，其用于检测高加索人群的九个耳聋基因突变，包括GJB2（c.35delG，c.167delT，c.132G> C和c.269D> C），GJB6（c。del309kb），SLC26A4（c.707T> C和c.1246A> C），12S rRNA（m.1555A> G和m.7444G> A）。 在另一方面，提供了一种方法，该方法包括用发光体标记靶分子，将靶分子偶联到颗粒上，并结合微阵列上的探针分子。 在某些方面，这种具有高灵敏度的技术能够以有效的方式检测和解释分子相互作用。

    LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS
    4.
    发明申请
    LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS 审中-公开
    与基于微量元素测定的颗粒偶联的荧光标记分子

    公开(公告)号：WO2015081612A1

    公开(公告)日：2015-06-11

    申请号：PCT/CN2014/001085

    申请日：2014-12-02

    Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

    Inventor： LI, Yang ,  ZHANG, Guanbin ,  JIANG, Di ,  XIANG, Guangxin ,  XING, Wanli ,  CHENG, Jing

    IPC: G01N33/58 ,  C12Q1/68

    CPC classification number: C12Q1/6837 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6883 ,  C12Q1/6886 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16 ,  G01N33/58 ,  C12Q2537/143 ,  C12Q2563/103 ,  C12Q2563/149

    Abstract: A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.

    Abstract translation: 使用微阵列芯片标记与颗粒偶联用于检测靶分子的靶分子的方法，包括：提供官能化微粒，其中所述微粒涂覆有一个或多个官能团; 在待检测的每个靶分子上提供修饰基团以形成修饰的靶分子; 使官能化的微粒与修饰的靶分子接触; 将发光体耦合到功能化微粒与修饰的靶分子之间的复合物上，由此用发光体直接或间接标记每个修饰的靶分子。 通过用发光体直接或间接标记目标分子，该方法降低荧光检测的成本，并避免衍生自传统荧光标记分子的PCR抑制。

    KITS AND METHODS FOR DETECTING DRUG-INDUCED DEAFNESS SUSCEPTIBILITY
    6.
    发明申请
    KITS AND METHODS FOR DETECTING DRUG-INDUCED DEAFNESS SUSCEPTIBILITY 审中-公开
    用于检测药物诱导的不饱和可疑性的试剂盒和方法

    公开(公告)号：WO2014190762A1

    公开(公告)日：2014-12-04

    申请号：PCT/CN2014/000548

    申请日：2014-05-30

    Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY

    Inventor： KONG, Yanling ,  XIANG, Guangxin ,  ZHANG, Xiaogang ,  LIANG, Shujian ,  XING, Wanli

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6883 ,  C12Q2600/106 ,  C12Q2600/156

    Abstract: The present invention provides a detection kit for detecting the mitochondrial gene C1494T and A1555G mutations associated with maternally inherited aminoglycoside-induced deafness. The kit comprises 2 pairs of primers with the nucleotide sequences set forth in SEQ ID NO: l, SEQ ID NO:2, SEQ ID NO:5, and SEQ ID NO:6, and 3 TaqMan-MGB probes with the nucleotide sequences set forth in SEQ ID NO:3, SEQ ID NO:4, and SEQ ID NO:7.

    Abstract translation: 本发明提供了用于检测与母体遗传的氨基糖苷诱导的耳聋有关的线粒体基因C1494T和A1555G突变的检测试剂盒。 试剂盒包含2对具有SEQ ID NO：1，SEQ ID NO：2，SEQ ID NO：5和SEQ ID NO：6所示核苷酸序列的引物和3个具有核苷酸序列的TaqMan-MGB探针 在SEQ ID NO：3，SEQ ID NO：4和SEQ ID NO：7中。

    MICROARRAY-BASED ASSAY INTEGRATED WITH PARTICLES FOR ANALYZING MOLECULAR INTERACTIONS
    8.
    发明申请
    MICROARRAY-BASED ASSAY INTEGRATED WITH PARTICLES FOR ANALYZING MOLECULAR INTERACTIONS 审中-公开
    用于分析分子相互作用的基于微阵列的测定

    公开(公告)号：WO2012016357A1

    公开(公告)日：2012-02-09

    申请号：PCT/CN2010/001203

    申请日：2010-08-06

    Applicant: CAPITALBIO CORPORATION ,  TSINGHUA UNIVERSITY ,  ZHANG, Guanbin ,  XIANG, Guangxin ,  XING, Wanli ,  CHENG, Jing

    Inventor： ZHANG, Guanbin ,  XIANG, Guangxin ,  XING, Wanli ,  CHENG, Jing

    IPC: C12Q1/68 ,  C12N15/11

    CPC classification number: C12Q1/6837 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2563/155

    Abstract: A microarray-based assay is provided, which is used for analyzing molecular interactions, including polynucleotides, polypeptides, antibodies, small molecule compounds, peptides and carbohydrates. Such method comprises coupling a target molecule to a particle and then binding to a probe molecule on microarray. In particular, multiplexed genetic analysis of nucleic acid fragments can be implemented. Specific genes, single nucleotide polymorphisms or gene mutations, such as deletions, insertions, and indels, can be identified. Coupled with microarray, the particles, themselves or further modified, facilitate the detection of results with non-expensive devices or even naked eyes. This technology enables the detection and interpretation of molecular interactions in an efficient and cost effective way.

    Abstract translation: 提供基于微阵列的测定法，其用于分析分子相互作用，包括多核苷酸，多肽，抗体，小分子化合物，肽和碳水化合物。 这种方法包括将靶分子偶联到颗粒上，然后与微阵列上的探针分子结合。 特别地，可以实现核酸片段的多重遗传分析。 可以鉴定特异性基因，单核苷酸多态性或基因突变，例如缺失，插入和插入。 与微阵列结合，颗粒本身或进一步修饰，有助于使用非昂贵装置甚至肉眼检测结果。 该技术能够以有效和成本有效的方式检测和解释分子相互作用。

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