公开(公告)号：WO2016061071A1

公开(公告)日：2016-04-21

申请号：PCT/US2015/055284

申请日：2015-10-13

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  ZHANG, Ying ,  HARMS, Lisa

IPC: C12N5/071

CPC classification number: C12N5/063 ,  A61K35/36 ,  C12N2500/38 ,  C12N2500/90 ,  C12N2501/01 ,  C12N2501/10 ,  C12N2501/11 ,  C12N2501/113 ,  C12N2501/15 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/33 ,  C12N2501/385 ,  C12N2501/998 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2533/52 ,  G01N33/5073

Abstract: Provided herein are methods for the generation of functional keratinocyte stem cells that are differentiated directly from human ESCs/iPSCs in a chemically defined serum-free cell culture system, as well as cells derived therefrom and methods of use thereof. Also provided are methods for culturing primary keratinocytes.

Abstract translation: 本文提供了用于产生在化学上限定的无血清细胞培养系统中直接与人ESCs / iPSC分化的功能性角化细胞干细胞的方法，以及由其衍生的细胞及其使用方法。 还提供了培养原代角质形成细胞的方法。

公开(公告)号：WO2017070333A1

公开(公告)日：2017-04-27

申请号：PCT/US2016/057893

申请日：2016-10-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  VODYANYK, Maksym, A. ,  SASAKI, Jeffrey ,  RAJESH, Deepika ,  BURTON, Sarah, A.

IPC: C12N5/0789

CPC classification number: C12N5/0647 ,  C12N5/0636 ,  C12N5/0639 ,  C12N5/0646 ,  C12N15/85 ,  C12N2500/90 ,  C12N2501/115 ,  C12N2501/125 ,  C12N2501/145 ,  C12N2501/155 ,  C12N2501/165 ,  C12N2501/2303 ,  C12N2501/2306 ,  C12N2501/2307 ,  C12N2501/26 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2501/727 ,  C12N2506/025 ,  C12N2506/11 ,  C12N2506/45 ,  C12N2510/00 ,  C12N2840/20

Abstract: The present disclosure generally regards methods and compositions for providing multi-lineage hematopoietic precursor cells from pluripotent stem cells (PSCs). The PSCs comprise an expression construct encoding an ETS/ERG gene, GATA2 and HOXA9. Also provided are methods for providing hematopoietic stem cells capable of long-term engraftment in mammals, such as humans. Further provided are therapeutic compositions including the provided hematopoietic stem cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects

Abstract translation: 本公开一般涉及用于从多能干细胞（PSC）提供多谱系造血前体细胞的方法和组合物。 PSC包含编码ETS / ERG基因，GATA2和HOXA9的表达构建体。 还提供了用于提供能够在哺乳动物（例如人）中长期植入的造血干细胞的方法。 进一步提供了治疗性组合物，包括所提供的造血干细胞和造血细胞的前体，以及用于治疗受试者的方法

公开(公告)号：WO2012109208A2

公开(公告)日：2012-08-16

申请号：PCT/US2012/024098

申请日：2012-02-07

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  YU, Junying ,  VODYANYK, Maksym, A.

Inventor： YU, Junying ,  VODYANYK, Maksym, A.

IPC: C12N5/074 ,  C12N5/10 ,  C12N15/63 ,  C12N5/02 ,  A61K35/12

CPC classification number: C12N5/0647 ,  A61K35/28 ,  C12N2501/60 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2506/45 ,  C12N2510/00

Abstract: The invention generally regards methods for providing ematopoietic cells and precursors of hematopoietic cells from a variety of cell sources, such as pluripotent stem cells or somatic cells. Also provided are therapeutic compositions including the provided hematopoietic cells and precursors of hematopoietic cells, and methods of using such for the treatment of subjects.

Abstract translation: 本发明通常涉及用于从多种细胞来源例如多能干细胞或体细胞提供造血细胞和造血细胞前体的方法。 还提供了治疗组合物，包括所提供的造血细胞和造血细胞的前体，以及用于治疗受试者的方法。

公开(公告)号：WO2011130402A2

公开(公告)日：2011-10-20

申请号：PCT/US2011/032309

申请日：2011-04-13

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  YU, Junying ,  CHAU, Fongching, Kevin ,  JIANG, Jinlan ,  JIANG, Yong ,  VODYANYK, Maksym, A.

Inventor： YU, Junying ,  CHAU, Fongching, Kevin ,  JIANG, Jinlan ,  JIANG, Yong ,  VODYANYK, Maksym, A.

IPC: C12N5/0735

CPC classification number: C12N15/85 ,  A61K35/407 ,  C12N5/067 ,  C12N2501/12 ,  C12N2501/237 ,  C12N2501/60 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5067

Abstract: The invention generally features methods for providing hepatocytes from a variety of cell sources, particularly pluripotent stem cells, therapeutic compositions featuring such cells, and methods of using them for the treatment of subjects.

Abstract translation: 本发明通常具有从各种细胞来源，特别是多能干细胞，特征为这种细胞的治疗组合物提供肝细胞的方法，以及使用它们用于治疗受试者的方法。

公开(公告)号：WO2015164228A1

公开(公告)日：2015-10-29

申请号：PCT/US2015/026583

申请日：2015-04-20

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  ZHANG, Xin

IPC: C12N5/071

CPC classification number: C12N5/067 ,  C12N15/85 ,  C12N2501/01 ,  C12N2501/237 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/60 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2510/00

Abstract: The present invention provides methods comprising both genetic and chemical means for the production of hepatocytes from a variety of cell sources, particularly pluripotent stem cells.

Abstract translation: 本发明提供了用于从各种细胞来源，特别是多能干细胞产生肝细胞的遗传和化学手段的方法。

公开(公告)号：WO2011056971A2

公开(公告)日：2011-05-12

申请号：PCT/US2010/055444

申请日：2010-11-04

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  YU, Junying

Inventor： YU, Junying

IPC: C12N5/074 ,  C12N5/02 ,  C12N15/85

CPC classification number: C12N5/0696 ,  A61K35/545 ,  C12N2501/10 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/235 ,  C12N2501/60 ,  C12N2501/70 ,  C12N2510/00 ,  C12N2533/52

Abstract: Methods and composition of induction of pluripotent stem cells are disclosed. For example, in certain aspects methods for generating essentially vector-free induced pluripotent stem cells with cell signaling regulators are described. Furthermore, certain aspects of the invention provide novel compositions comprising induced pluripotent stem cells essentially free of exogenous retroviral vector elements in the presence of a medium comprising signaling inhibitors. In certain aspects, feeder-free episomal reprogramming methods may be provided.

Abstract translation: 公开了诱导多能干细胞的方法和组合物。 例如，在某些方面描述了用细胞信号传导调节剂产生基本上无载体的诱导性多能干细胞的方法。 此外，本发明的某些方面提供了新型组合物，其包含在包含信号传导抑制剂的培养基存在下基本上不含外源逆转录病毒载体元件的诱导性多能干细胞。 在某些方面，可以提供无饲养细胞的附加体重编程方法。

公开(公告)号：WO2014130770A1

公开(公告)日：2014-08-28

申请号：PCT/US2014/017588

申请日：2014-02-21

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC.

Inventor： YU, Junying ,  ZHANG, Xin

IPC: C12N5/071

CPC classification number: C12N15/85 ,  C12N5/067 ,  C12N2501/01 ,  C12N2501/237 ,  C12N2501/33 ,  C12N2501/39 ,  C12N2501/727 ,  C12N2501/999 ,  C12N2506/02 ,  C12N2510/00 ,  G01N33/5067

Abstract: The present invention provides methods comprising both genetic and chemical means for the production of hepatocytes from a variety of cell sources, particularly pluripotent stem cells.

Abstract translation: 本发明提供了用于从各种细胞来源，特别是多能干细胞产生肝细胞的遗传和化学手段的方法。

公开(公告)号：WO2012006440A2

公开(公告)日：2012-01-12

申请号：PCT/US2011/043218

申请日：2011-07-07

Applicant: CELLULAR DYNAMICS INTERNATIONAL, INC. ,  YU, Junying ,  VODYANYK, Maksym, A.

Inventor： YU, Junying ,  VODYANYK, Maksym, A.

IPC: C12N5/071 ,  C12N5/10 ,  C12N15/12 ,  A61K35/12 ,  A61P7/06 ,  A61P9/00

CPC classification number: C12N15/85 ,  C12N5/069 ,  C12N5/0692 ,  C12N2501/40 ,  C12N2506/02 ,  C12N2506/45 ,  G01N33/5064 ,  G01N2500/10

Abstract: The invention generally regards methods for providing endothelial cells and precursors of endothelial cells from a variety of cell sources, such as pluripotent stem cells. Also provided are therapeutic compositions including the provided endothelial cells, and methods of using them for the treatment of subjects.

Abstract translation: 本发明通常涉及从多种细胞来源（例如多能干细胞）提供内皮细胞和内皮细胞前体的方法。 还提供包括所提供的内皮细胞的治疗组合物，以及使用它们来治疗受试者的方法。