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    USE OF A BACILLUS COMPOSITION FOR INCREASING THE AMOUNT OF AVAILABLE SUGARS IN ANIMAL FEED
    3.
    发明申请
    USE OF A BACILLUS COMPOSITION FOR INCREASING THE AMOUNT OF AVAILABLE SUGARS IN ANIMAL FEED 审中-公开
    用于增加动物饲料中可利用糖的量的组合物

    公开(公告)号：WO2014187955A1

    公开(公告)日：2014-11-27

    申请号：PCT/EP2014/060661

    申请日：2014-05-23

    Applicant: CHR. HANSEN A/S

    Inventor： NIELSEN, Beatrice ,  STYRISHAVE, Tina ,  CANTOR, Mette Dines ,  DERKX, Patrick ,  NIELSEN, Jonna ,  LANTZ, Robert

    IPC: A23K1/00 ,  A23K1/18

    CPC classification number: C12Q1/02 ,  A23K10/18 ,  A23K20/163 ,  A23K50/30 ,  A23K50/60 ,  A23K50/75 ,  G01N2333/32

    Abstract: The present invention relates to a method for assaying whether a Bacillus composition comprising at least one Bacillus strain is capable of increasing the amount of available sugar from animal feed comprising non-starch polysaccharides (NSP), said method comprising in cubating the animal feed with the Bacillus composition at 37°C for 24 hours, measuring the absorbance in a dinitrosalicylic acid (DNS) assay at OD540 and calculating the amount of sugar (hexose) equivalents produced by correlating the measured absorbance to a standard curve. Based upon this method the invention provides an animal feed comprising 14% (w/w) or more of non-starch polysaccharides (NSP) if the animal feed is for a piglet, lactating sow, broiler or layer or 19% (w/w) or more of non-starch polysaccharides (NSP) if the animal feed is for a grower-finisher pig or gestating sow, and a Bacillus composition comprising at least one Bacillus strain, as well as methods and uses relating thereto.

    Abstract translation: 本发明涉及用于测定包含至少一种芽孢杆菌菌株的芽孢杆菌组合物是否能够增加包含非淀粉多糖（NSP）的动物饲料中的可用糖的量的方法，所述方法包括将动物饲料与 芽孢杆菌组合物在37℃下培养24小时，测量OD540中二硝基水杨酸（DNS）测定中的吸光度，并计算通过将测量的吸光度与标准曲线相关而产生的糖（己糖）当量。 基于该方法，如果动物饲料用于仔猪，哺乳母猪，肉鸡或蛋白质或19％（w / w），本发明提供了包含14％（w / w）或更多的非淀粉多糖（NSP）的动物饲料 ）或更多的非淀粉多糖（NSP），如果动物饲料用于种植者育种猪或妊娠母猪，以及包含至少一种芽孢杆菌菌株的芽孢杆菌组合物，以及与其相关的方法和用途。

    ANTIBIOTIC SENSITIVE BACILLUS STRAINS HAVING ANTIMICROBIAL EFFECT AGAINST E. COLI AND CLOSTRIDIUM PERFRINGENS AND HAVING HIGH SPORULATION CAPACITY
    4.
    发明申请
    ANTIBIOTIC SENSITIVE BACILLUS STRAINS HAVING ANTIMICROBIAL EFFECT AGAINST E. COLI AND CLOSTRIDIUM PERFRINGENS AND HAVING HIGH SPORULATION CAPACITY 审中-公开
    具有抗大肠杆菌和隐孢子虫的抗生素敏感菌株具有高运动能力的抗生素敏感菌株

    公开(公告)号：WO2013153159A1

    公开(公告)日：2013-10-17

    申请号：PCT/EP2013/057590

    申请日：2013-04-11

    Applicant: CHR. HANSEN A/S

    Inventor： NIELSEN, Beatrice ,  CANTOR, Mette Dines ,  STUER-LAURIDSEN, Birgitte ,  DERKX, Patrick ,  JOHANSEN, Eric

    IPC: C12N1/20 ,  C12N15/01 ,  A23K1/00 ,  A23L1/30 ,  C12R1/07 ,  C12R1/125

    CPC classification number: A61K35/742 ,  A23K10/18 ,  C12N1/20 ,  C12N15/01 ,  C12R1/07 ,  C12R1/125

    Abstract: A Bacillus strain characterized by (i) : sensitivity for ampicillin, vancomycin, gentamicin, kanamycin, streptomycin, erythromycin, clindamycin, tetracycline and chloramphenicol; (ii) antimicrobial activity against E. coli and Clostridium perfringens ; and (iii) a sporulation percentage of at least 80 when measured after 2 days of incubation. The invention further relates to a method for selecting such strains. Many of the identified strains according to the invention are of the species Bacillus amyloliquefaciens . Some of the Bacillus amyloliquefaciens were further identified as Bacillus amyloliquefaciens subsp. amyloliquefaciens whereas others were identified as amyloliquefaciens subsp. plantarum. A Bacillus strain of the invention may be used as a feed additive to animal feed where it has a probiotic effect.

    Abstract translation: 一种芽孢杆菌菌株，其特征在于：（i）：对氨苄青霉素，万古霉素，庆大霉素，卡那霉素，链霉素，红霉素，克林霉素，四环素和氯霉素的敏感性; （ii）对大肠杆菌和产气荚膜梭菌的抗菌活性; 和（iii）孵育2天后测量的至少80的孢子形成百分比。 本发明还涉及一种选择这种菌株的方法。 根据本发明的许多鉴定的菌株是物种解淀粉芽孢杆菌。 一些解淀粉芽孢杆菌进一步鉴定为解淀粉芽孢杆菌亚种。 解淀粉芽孢杆菌，而其他被鉴定为解淀粉芽孢杆菌亚种。 植物。 本发明的芽孢杆菌菌株可以用作动物饲料的饲料添加剂，其具有益生菌作用。

    NEW BACTERIA
    6.
    发明申请
    NEW BACTERIA 审中-公开
    新的细菌

    公开(公告)号：WO2018041717A1

    公开(公告)日：2018-03-08

    申请号：PCT/EP2017/071352

    申请日：2017-08-24

    Applicant: CHR. HANSEN A/S

    Inventor： DERKX, Patrick ,  JANZEN, Thomas ,  SOERENSEN, Kim Ib

    IPC: C12N1/20 ,  A23C9/123 ,  C07K14/315 ,  C12R1/46

    Abstract: The present invention relates to a bacterial cell with texturizing property, starter cultures comprising the cell, and dairy products manufactured using the bacterial cell.

    Abstract translation: 本发明涉及具有组织化性质的细菌细胞，包含该细胞的起子培养物和使用该细菌细胞制造的乳制品。

    A SCREENING METHOD FOR RHEOLOGICAL PROPERTIES OF MILK GEL
    8.
    发明申请
    A SCREENING METHOD FOR RHEOLOGICAL PROPERTIES OF MILK GEL 审中-公开
    一种用于牛奶凝胶流变特性的筛选方法

    公开(公告)号：WO2015086574A1

    公开(公告)日：2015-06-18

    申请号：PCT/EP2014/076999

    申请日：2014-12-09

    Applicant: CHR. HANSEN A/S

    Inventor： CANTOR, Mette ,  POULSEN, Vera ,  OEREGAARD, Gunnar ,  TRIHAAS, Jeorgos ,  WIND, Sandra ,  DERKX, Patrick

    IPC: A23C9/00 ,  A23C9/12 ,  G01N11/00 ,  G01N11/06 ,  G01N11/08 ,  A23C9/154 ,  A23C21/00 ,  G01N35/10

    CPC classification number: G01N11/02 ,  A23C9/123 ,  A23C13/16 ,  A23C17/00 ,  A23C19/076 ,  G01N7/00 ,  G01N11/08 ,  G01N33/04 ,  G01N2203/0089

    Abstract: The present invention relates to the field of dairy technology, in particular it relates to a method for assessing rheological properties of acidified milk gels (milk gels), including determination of shear stress, gel firmness and water-holding capacity. The method can be used to determine rheological properties of acidified milk, e.g. yoghurt and fresh cheese, in a fast and reliable way. The present invention also relates to a method of screening for microbial cultures resulting in fermented milk with desired rheological properties. By using an automated microtiter-plate pipetting station equipped with a pressure sensor inside the air displacement barrel of each pipette, it is possible to monitor real-time changes in pressure, when aspirating and dispensing milk gels, and then correlate the pressure versus time data obtained with milk gel rheological properties such as shear stress, gel firmness and water-holding capacity.

    Abstract translation: 本发明涉及乳制品技术领域，特别涉及一种评估酸化乳胶（乳胶）流变特性的方法，包括测定剪切应力，凝胶坚固性和持水能力。 该方法可用于测定酸化牛奶的流变性质，例如， 酸奶和新鲜的奶酪，以快速可靠的方式。 本发明还涉及一种筛选微生物培养物的方法，该方法产生具有所需流变特性的发酵乳。 通过使用在每个移液管的排气筒内装有压力传感器的自动化微量滴定板移液站，可以在抽吸和分配牛奶凝胶时监测压力的实时变化，然后将压力与时间数据相关联 获得牛奶凝胶流变性能如剪切应力，凝胶坚固度和保水能力。

    METHOD OF PRODUCING STREPTOCOCCUS THERMOPHILUS MUTANT STRAINS
    9.
    发明申请
    METHOD OF PRODUCING STREPTOCOCCUS THERMOPHILUS MUTANT STRAINS 审中-公开

    公开(公告)号：WO2018220104A1

    公开(公告)日：2018-12-06

    申请号：PCT/EP2018/064325

    申请日：2018-05-31

    Applicant: CHR. HANSEN A/S

    Inventor： TRIHAAS, Jeorgos ,  JANZEN, Thomas ,  DERKX, Patrick

    IPC: C12N1/20 ,  C12N15/01 ,  C12P1/04 ,  C12R1/46 ,  A23C9/12 ,  A23C9/123

    Abstract: The present invention is directed to a method of producing a Streptococcus thermophilus strain comprising the steps of a) Providing a mother strain in the form of Streptococcus thermophilus DSM32502, b) Growing a culture of the mother strain in the presence of a bacteriophage, to which the mother strain is not resistant, to obtain a number of mutant strains, which are resistant to the said bacteriophage, c) Measuring the acidification time of the bacteriophage-resistant mutant strains and the mother strain in a milk base and selecting at least one mutant strain with a reduced acidification time as compared to the mother strain to obtain a fast-acidifying mutant strain.

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