公开(公告)号：WO2005079544A9

公开(公告)日：2007-09-20

申请号：PCT/US2005005743

申请日：2005-02-22

Applicant: CYVERA CORP ,  SIRKIS JAMES S ,  MOON JOHN A ,  BRUCATO ROBERT N ,  PUTNAM MARTIN A ,  LI TUO ,  TRAYNOR JOSEPH

Inventor： SIRKIS JAMES S ,  MOON JOHN A ,  BRUCATO ROBERT N ,  PUTNAM MARTIN A ,  LI TUO ,  TRAYNOR JOSEPH

IPC: G01B11/00 ,  B01L3/00 ,  B32B5/02 ,  B32B27/04 ,  B32B27/12 ,  C12M1/34 ,  C12M1/36 ,  C12M1/38 ,  C12M3/00 ,  G01B11/14 ,  G01N1/00 ,  G01N21/00 ,  G01N21/01 ,  G01N21/27 ,  G01N21/29 ,  G01N21/31 ,  G01N21/41 ,  G01N21/47 ,  G01N21/59 ,  G01N21/64 ,  G01N21/66

CPC classification number: G01N21/6452 ,  B01J2219/005 ,  B01J2219/0054 ,  B01J2219/00549 ,  B01J2219/0056 ,  B01J2219/00576 ,  B01J2219/00722 ,  B01L3/5025 ,  B01L3/502761 ,  B01L2200/0605 ,  B01L2200/0668 ,  B01L2300/0803 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0487

Abstract: A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (µm) in diameter and between 100 and 500 µm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes. The third axis, rotation about the center axis of the cylinder, is azimuthally symmetric and therefore does not require alignment.

Abstract translation: 提供了一种用于对准光学元件或微珠的方法和装置，其中每个微珠具有细长体，其沿着其纵向轴线嵌入其中的代码，以被代码读取装置读取。 微珠与定位装置对准，使得微珠的纵向轴线相对于代码读取装置定位成固定的取向。 微珠通常为直径为25至250微米（μm）且长度为100至500μm的圆柱形玻璃珠，并且具有嵌入珠的中心区域中的全息代码，其用于从其余部分识别它 珠子中有一批具有许多不同化学探针的珠粒。 交叉参考用于确定哪个探针连接到哪个珠，从而允许研究者将每个珠上的化学成分与测量的荧光信号相关联。 由于代码由通常沿轴线设置的衍射光栅组成，因此在三个旋转轴中的两个中存在入射读出激光束和读出检测器之间所需的特定对准。 第三轴，围绕气缸的中心轴线的旋转是方位对称的，因此不需要对准。

公开(公告)号：WO2005029047A3

公开(公告)日：2005-06-09

申请号：PCT/US2004030038

申请日：2004-09-13

Applicant: CYVERA CORP ,  PUTNAM MARTIN A ,  LI TUO ,  MOON JOHN A

Inventor： PUTNAM MARTIN A ,  LI TUO ,  MOON JOHN A

IPC: B01L3/14 ,  G01N15/14 ,  G01N35/00 ,  G02B5/18 ,  G02B6/02 ,  G02B6/16

CPC classification number: G01N35/00732 ,  G01N2035/00772

Abstract: An assay stick (7) includes a transparent reaction vessel or tube (14) having one or more microbeads (8) disposed therein. The microbeads (8) have a plurality of unique identification digital codes based on a diffraction grating (12) disposed therein that are detected when illuminated by incident light (24). The incident light (24) may be directed transversely onto the side or onto an end of the tube (14) with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The assay stick (7) may be reused or disposed upon completion of the assay. Alternatively, the beads may be attached to a strip or planar surface. The encoded beads can also provide traceability, quality-control, and authenticity of each bead (8) to its source and/or to the chemistry on each bead (8). Also, the low sample volume of the assay stick allows for faster reactions and better sensitivity.

Abstract translation: 测定棒（7）包括其中设置有一个或多个微珠（8）的透明反应容器或管（14）。 微珠（8）具有基于设置在其中的衍射光栅（12）的多个唯一的识别数字代码，其被入射光（24）照射时检测。 入射光（24）可以以窄带（单波长）或多波长源横向地引导到管（14）的一侧或一端上，在这种情况下，代码由光的空间分布或 波长谱。 测定棒（7）可以在测定完成时重复使用或处置。 或者，珠可以附接到条或平面表面。 编码的珠粒还可以提供每个珠粒（8）对其来源和/或每个珠粒上的化学物质的追踪性，质量控制和真实性（8）。 此外，测定棒的低样品体积允许更快的反应和更好的灵敏度。

公开(公告)号：WO2004025344A8

公开(公告)日：2004-05-21

申请号：PCT/US0328890

申请日：2003-09-12

Applicant: CYVERA CORP

Inventor： PUTNAM MARTIN A ,  MOON JOHN ,  SZCZEPANEK PAUL S ,  LI TUO ,  RAUSEO ANTHONY ,  TRAYNOR JOSEPH

IPC: B01J19/00 ,  B01L3/00 ,  G01N15/14 ,  G01N33/52 ,  G02B5/18 ,  G02B6/34 ,  G03H1/00 ,  G06K9/18 ,  G06T5/00 ,  G02B6/16

CPC classification number: G06K19/06009 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/502707 ,  B01L3/5085 ,  B01L2200/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0487 ,  G02B6/02076 ,  G03H1/0272 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24

Abstract: A method for manufacturing a diffusion grating-based optical identification element is provided. The optical identification element includes a known optical substrate, having an optical diffraction grating disposed in the volume of the substrate. A large number of substrates or microbeads having unique identification codes can be manufactured winding a substrate, such as a fiber, around a polygonal shaped cage/basket to form a fiber ribbon having flatsections. A grating writing station writes one or more gratings into each flat section to form a unique code to this section. Each flat section of fibers of the fiber ribbon is written with the saure gratings to provide the same identification code, or alternatively each flat section may be have a different grating(s) written therein so that each section has a different identification code. The fiber ribbon is then removed from the cage and diced to form a groups of optical identification elements, each group having unique optical identification codes.

Abstract translation: 提供了一种制造基于漫射光栅的光学识别元件的方法。 光学识别元件包括已知的光学基板，其具有布置在基板的体积中的光学衍射光栅。 可以制造具有独特识别码的大量衬底或微珠，其围绕多边形形状的笼/筐缠绕诸如纤维等基底以形成具有扁平截面的纤维带。 光栅书写站将一个或多个光栅写入每个平面部分，以形成该部分的唯一代码。 光纤带的每个平坦部分的纤维都用琢型光栅写入以提供相同的识别码，或者每个平坦部分可以具有写在其中的不同光栅，使得每个部分具有不同的识别码。 然后将光纤带从笼中取出并切割成一组光学识别元件，每组具有唯一的光学识别码。

公开(公告)号：WO2005026729A3

公开(公告)日：2005-06-30

申请号：PCT/US2004030300

申请日：2004-09-13

Applicant: CYVERA CORP

Inventor： LI TUO ,  MOON JOHN A ,  PUTNAM MARTIN A ,  PERBOST MICHEL ,  TRAYNOR JOSEPH

IPC: B01J19/00 ,  G01N33/543 ,  G01N33/552 ,  G02B5/18 ,  G02B6/34 ,  G03H1/00 ,  G06K9/18 ,  G06K19/00 ,  G06K19/06 ,  G06T5/00

CPC classification number: G01N33/552 ,  G01N21/4788 ,  G01N21/6428 ,  G01N21/645 ,  G01N21/6452 ,  G01N21/7743 ,  G01N33/54306 ,  G01N33/54313 ,  G01N33/54366 ,  G01N33/54386 ,  G03H1/0005 ,  G03H1/02 ,  G03H1/0236 ,  G03H1/26 ,  G03H2001/0033 ,  G03H2001/0044 ,  G03H2210/53 ,  G03H2210/63 ,  G03H2230/10 ,  G03H2250/12 ,  G06K19/04 ,  G06K19/06009

Abstract: A method and apparatus are provided for aligning optical elements or microbeads (8), wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads (8) are aligned with a positioning device (or cell) (500) having a plate or platform (200, 1252) with grooves (205, 1258) so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads (8) are typically cylindrically shaped glass beads having a diffraction grating-based code embedded in the bead (8) disposed along an axis, which requires a predetermined alignment between the incident code readout laser beam and the code readout detector in two of three rotational axes. The geometry of the grooves (205) are designed to allow for easy loading and unloading of beads from a cell, and the grooves (205) may be straight or curved. Also, the cell may be segmented into regions each associated with a different reaction or used for a different identification process/ application, and may have many different geometries depending on the application.

Abstract translation: 提供了一种用于对准光学元件或微珠（8）的方法和装置，其中每个微珠具有细长体，其沿着其纵向轴线嵌入其中的代码，以被代码读取装置读取。 微珠（8）与具有带槽（205,1258）的板或平台（200,1252）的定位装置（或电池）（500）对齐，使得微珠的纵向轴线相对于 代码阅读器。 微珠（8）通常为圆柱形玻璃珠，其具有嵌入沿着轴设置的珠（8）中的基于衍射光栅的代码，其需要在入射代码读出激光束和代码读出检测器之间的预定对准 三个旋转轴。 凹槽（205）的几何形状被设计成允许从电池容易地加载和卸载珠，并且凹槽（205）可以是直的或弯曲的。 此外，电池可以被分割成各自与不同反应相关联的区域或用于不同的识别过程/应用，并且可以根据应用具有许多不同的几何形状。

公开(公告)号：WO2004024328B1

公开(公告)日：2004-06-03

申请号：PCT/US0328862

申请日：2003-09-12

Applicant: CYVERA CORP

Inventor： MOON JOHN ,  PUTNAM MARTIN A ,  LI TUO ,  TRAYNOR JOSEPH

IPC: B01J19/00 ,  B01L3/00 ,  G01N1/00 ,  G01N15/14 ,  G01N33/52 ,  G02B6/34 ,  G03H1/00 ,  G06K9/18 ,  G06T5/00

CPC classification number: G03H1/0272 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0487 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24 ,  Y10T436/25

Abstract: A method and apparatus are provided for aligning optical elements or microbeads, wherein each microbead has an elongated body with a code embedded therein along a longitudinal axis thereof to be read by a code reading device. The microbeads are aligned with a positioning device so the longitudinal axis of the microbeads is positioned in a fixed orientation relative to the code reading device. The microbeads are typically cylindrically shaped glass beads between 25 and 250 microns (µm) in diameter and between 100 and 500 µm long, and have a holographic code embedded in the central region of the bead, which is used to identify it from the rest of the beads in a batch of beads with many different chemical probes. A cross reference is used to determine which probe is attached to which bead, thus allowing the researcher to correlate the chemical content on each bead with the measured fluorescence signal. Because the code consists of a diffraction grating typically disposed along an axis, there is a particular alignment required between the incident readout laser beam and the readout detector in two of the three rotational axes. The third axis, rotation about the center axis of the cylinder, is azimuthally symmetric and therefore does not require alignment.

Abstract translation: 提供了一种用于对准光学元件或微珠的方法和装置，其中每个微珠具有细长体，其沿着其纵向轴线嵌入其中的代码，以被代码读取装置读取。 微珠与定位装置对准，使得微珠的纵向轴线相对于代码读取装置定位成固定的取向。 微珠通常为直径为25至250微米（μm）且长度为100至500μm的圆柱形玻璃珠，并且具有嵌入珠的中心区域中的全息代码，其用于从其余部分识别它 珠子中有一批具有许多不同化学探针的珠粒。 交叉参考用于确定哪个探针连接到哪个珠，从而允许研究者将每个珠上的化学成分与测量的荧光信号相关联。 由于代码由通常沿轴线设置的衍射光栅组成，因此在三个旋转轴中的两个中存在入射读出激光束和读出检测器之间所需的特定对准。 第三轴，围绕气缸的中心轴线的旋转是方位对称的，因此不需要对准。

公开(公告)号：WO2004025562B1

公开(公告)日：2004-06-10

申请号：PCT/US0329164

申请日：2003-09-12

Applicant: CYVERA CORP

Inventor： MOON JOHN ,  PUTNAM MARTIN A ,  KERSEY ALAN D ,  LI TUO ,  DUNPHY JAMES ,  DIDDEN F KEVIN

IPC: B01J19/00 ,  B01L3/00 ,  G01N15/14 ,  G01N33/52 ,  G02B5/18 ,  G02B6/34 ,  G02B27/44 ,  G03H1/00 ,  G06K9/18 ,  G06T5/00

CPC classification number: G03H1/0272 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0487 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24

Abstract: A methods and apparatus for labeling an item using diffraction grating-based encoded optical identification elements (8) includes an optical substrate (10) having at least one diffraction grating (12) disposed therein. The grating (12) has one or more colocated pitches A which represent a unique identification digital code that is detected when illuminated by incident light (24). The incident light (24) may be directed transversely from the side of the substrate (10) (or from an end) with a narrow band (single wavelength) or multiple wavelength source, and the code is represented by a spatial distribution of light or a wavelength spectrum, respectively, or a combination thereof. The element (8) can provide a large number of unique codes, e.g., greater than 67 million codes, and can withstand harsh environments. The encoded element (8) may be used to label any desired item, such as large or small objects, products, solids, powders, liquids, gases, plants, minerals, cells and/or animals, or any combination of or portion of one or more thereof. The label may be used for many different purposes, such as for sorting, tracking, identification, verification, authentication, anti-theft/anti-counterfeit, security/anti-terrorism, or for other purposes. In a manufacturing environment, the elements (8) may be used to track inventory for production information or sales of goods/products.

Abstract translation: 使用基于衍射光栅的编码光学识别元件（8）来标记物品的方法和设备包括具有设置在其中的至少一个衍射光栅（12）的光学基板（10）。 光栅（12）具有一个或多个共同定位的间距A，其表示当被入射光（24）照射时检测到的唯一识别数字码。 入射光（24）可以从具有窄带（单波长）或多个波长源的基板（10）侧（或从一端）横向引导，并且代码由光的空间分布或 波长谱，或其组合。 元件（8）可以提供大量唯一的代码，例如大于67百万个代码，并且可以承受恶劣的环境。 编码元件（8）可用于标记任何所需物品，例如大或小物体，产品，固体，粉末，液体，气体，植物，矿物，细胞和/或动物，或任何组合或一部分 或更多。 该标签可用于许多不同的目的，例如用于分类，跟踪，识别，验证，认证，防盗/防伪，安全/反恐，或用于其他目的。 在制造环境中，元件（8）可用于跟踪生产信息的库存或货物/产品的销售。

公开(公告)号：WO2004025560B1

公开(公告)日：2004-06-10

申请号：PCT/US0328875

申请日：2003-09-12

Applicant: CYVERA CORP

Inventor： PUTNAM MARTIN A ,  MOON JOHN ,  LI TUO

IPC: B01J19/00 ,  B01L3/00 ,  C12M1/34 ,  C12Q1/68 ,  G01N15/14 ,  G01N33/52 ,  G01N33/53 ,  G02B6/34 ,  G03H1/00 ,  G06K9/18 ,  G06T5/00

CPC classification number: G03H1/0272 ,  B01J2219/00459 ,  B01J2219/00463 ,  B01J2219/0047 ,  B01J2219/005 ,  B01J2219/00547 ,  B01J2219/00576 ,  B01J2219/00702 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0636 ,  B01L2200/0668 ,  B01L2300/0654 ,  B01L2400/0409 ,  B01L2400/0457 ,  B01L2400/0487 ,  G03H2210/53 ,  G03H2230/10 ,  G03H2270/20 ,  G03H2270/24

Abstract: An assay stick 7 includes a transparent reaction vessel or tube 14 having one or more microbeads 8 disposed therein. The microbeads 8 have a plurality of unique identification digital codes based on a diffraction grating 12 disposed therein that are detected when illuminated by incident light 24. The incident light 24 may be directed transversely onto the side or onto an end of the tube 14 with a narrow band (single wavelength) or multiple wavelength source, in which case the code is represented by a spatial distribution of light or a wavelength spectrum, respectively. The assay stick 7 may be reused or disposed upon completion of the assay. Instead of beads, cells or molecules may be spotted or grown directly on the tube.

Abstract translation: 测定棒7包括其中设置有一个或多个微珠8的透明反应容器或管14。 微珠8具有基于设置在其中的衍射光栅12的多个独特的识别数字代码，其被入射光24照射时检测。入射光24可以横向地被引导到管14的一侧或一端上， 窄带（单波长）或多波长源，在这种情况下，码分别由光或波长光谱的空间分布表示。 测定棒7可以在测定完成后重复使用或放置。 代替珠，细胞或分子可以直接点在管上或在管上生长。