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    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN
    1.
    发明申请
    ENHANCED AMYLASE PRODUCTION BY N-TERMINAL ADDITION TO MATURE AMYLASE PROTEIN 审中-公开
    通过N末端添加成熟的淀粉酶蛋白增强了淀粉酶的生产

    公开(公告)号：WO2008118377A3

    公开(公告)日：2008-10-02

    申请号：PCT/US2008/003778

    申请日：2008-03-20

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  ENGLAND, George ,  KOLKMAN, Marc ,  VROEMEN, Casper

    Inventor： ENGLAND, George ,  KOLKMAN, Marc ,  VROEMEN, Casper

    IPC: C12N15/52

    Abstract: A method of making a Bacillus alpha-amylase variant that increases alpha-amylase production and the alpha-amylases produced thereby. The recombinant alpha-amylases can be placed in compositions and used for purposes of laundry detergents, cleaning and dishwashing detergents, fabric desizing, starch liquefaction, cereal liquefaction, starch saccharification, biofilm removal, and starch hydrolysis in cane sugar processing.

    Abstract translation: 制备增加α-淀粉酶产生的芽孢杆菌属α-淀粉酶变体和由此产生的α-淀粉酶的方法。 可以将重组α-淀粉酶置于组合物中，并用于蔗糖加工中的洗衣洗涤剂，清洁和洗碟洗涤剂，织物退浆，淀粉液化，谷物液化，淀粉糖化，生物膜去除和淀粉水解。

    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY
    2.
    发明申请
    PULLULANASE VARIANTS WITH INCREASED PRODUCTIVITY 审中-公开
    PULLULANASE变量具有提高的生产力

    公开(公告)号：WO2008024372A2

    公开(公告)日：2008-02-28

    申请号：PCT/US2007/018523

    申请日：2007-08-21

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  ENGLAND, George ,  KOLKMAN, Marc ,  MILLER, Brian S. ,  VROEMEN, Casper

    Inventor： ENGLAND, George ,  KOLKMAN, Marc ,  MILLER, Brian S. ,  VROEMEN, Casper

    IPC: C12N9/44 ,  C12N15/12 ,  C12N5/10

    CPC classification number: C12Y302/01041 ,  C12N9/2457

    Abstract: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of “wild-type” molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.

    Abstract translation: 本发明涉及酶促支链淀粉酶的新变体，编码所述新肽的基因序列，包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物。 通过使用密码子优化程序，选择性截短“野生型”分子和通过置换所选择的氨基酸残基，凭经验提供本发明的新型支链淀粉酶变体。 此外，本发明涉及这些新型支链淀粉酶肽在纺织，发酵，食品和其它工业中的用途。

    ENHANCED PROTEIN EXPRESSION AND METHODS THEREOF
    3.
    发明申请
    ENHANCED PROTEIN EXPRESSION AND METHODS THEREOF 审中-公开
    增强的蛋白质表达及其方法

    公开(公告)号：WO2017075195A1

    公开(公告)日：2017-05-04

    申请号：PCT/US2016/059078

    申请日：2016-10-27

    Applicant: DANISCO US INC

    Inventor： BONGIORNI, Cristina ,  CERVIN, Marguerite A. ,  ENGLAND, George ,  ZHU, Chao ,  KOOPMAN, Frank Wouter

    IPC: C12N1/20 ,  C12N9/12

    CPC classification number: C12N9/1247 ,  C12P21/00 ,  C12P21/02

    Abstract: The present disclosure is generally related to modified Gram positive bacterial cells producing increased amounts of one or more protein(s) of interest and modified Gram positive bacterial cells having increased genetic competency. Thus, certain embodiments of the disclosure are directed to modified Gram positive bacterial cells expressing an increased amount of a protein of interest, relative to an unmodified (parental) Gram positive bacterial cell expressing the same protein of interest, wherein the modified bacterial cell comprises at least one mutation in a rpo C gene encoding a variant RNA-polymerase (RNAP) β'-subunit polypeptide. In certain embodiments, the rpo C gene encoding the variant β'-subunit polypeptide is integrated into the chromosome of the modified cell. In other embodiments, the rpo C gene encoding the variant β-subunit polypeptide is comprised on an extrachromosomal plasmid introduced into the modified cell. In other embodiments, the disclosure is directed to competent Bacillus host cells comprising at least one copy of a nucleic acid construct encoding a modified rpoC polypeptide comprising 90% sequence identity to SEQ ID NO: 8 and an aspartic acid to glycine substitution at position 796 of SEQ ID NO: 8, wherein the polynucleotide encoding the rpoC polypeptide is foreign to the Bacillus host cell that was non-competent prior to the introduction of the first nucleic acid construct.

    Abstract translation: 本公开内容通常涉及产生增加量的一种或多种感兴趣蛋白质和修饰的具有增加的遗传能力的革兰氏阳性细菌细胞的修饰的革兰氏阳性细菌细胞。 因此，本公开的某些实施方案涉及相对于表达相同目的蛋白的未修饰（亲本）革兰氏阳性细菌细胞表达增加量的感兴趣蛋白的修饰的革兰氏阳性细菌细胞，其中修饰的细菌细胞包含at 编码变异RNA聚合酶（RNAP）β'-亚基多肽的rpoC基因中的至少一个突变。 在某些实施方案中，编码变体β'-亚基多肽的rpoC基因整合到修饰细胞的染色体中。 在其他实施方案中，编码变体β-亚基多肽的rpoC基因包含在引入修饰细胞中的染色体外质粒中。 在其他实施方案中，本公开涉及感受态芽孢杆菌宿主细胞，其包含编码修饰的rpoC多肽的核酸构建体的至少一个拷贝，所述修饰的rpoC多肽包含与SEQ ID NO：8具有90％序列同一性并且天冬氨酸与甘氨酸取代在位置796 SEQ ID NO：8，其中编码rpoC多肽的多核苷酸对于在引入第一核酸构建体之前是非感受态的芽孢杆菌属宿主细胞是外源的。

    CONDITIONING BIOMASS FOR MICROBIAL GROWTH
    7.
    发明申请
    CONDITIONING BIOMASS FOR MICROBIAL GROWTH 审中-公开
    微生物生长调节生物量

    公开(公告)号：WO2008085356A1

    公开(公告)日：2008-07-17

    申请号：PCT/US2007/025911

    申请日：2007-12-17

    Applicant: DANISCO US, INC., GENENCOR DIVISION ,  BODIE, Elizabeth A. ,  ENGLAND, George

    Inventor： BODIE, Elizabeth A. ,  ENGLAND, George

    IPC: C12N1/22 ,  C12P21/00 ,  C12N9/02 ,  C12N9/42 ,  C12R1/885

    CPC classification number: C12N1/22 ,  C12N1/38 ,  C12N9/001 ,  C12N9/0059 ,  C12N9/0061 ,  C12N9/0071 ,  C12N9/2437

    Abstract: The present invention relates to methods for improving the yield of microbial processes that use lignocellulose biomass as a nutrient source. The methods comprise conditioning a composition comprising lignocellulose biomass with an enzyme composition that comprises a phenol oxidizing enzyme. The conditioned composition can support a higher rate of growth of microorganisms in a process. In one embodiment, a laccase composition is used to condition lignocellulose biomass derived from non-woody plants, such as corn and sugar cane. The invention also encompasses methods for culturing microorganisms that are sensitive to inhibitory compounds in lignocellulose biomass. The invention further provides methods of making a product by culturing the production microorganisms in conditioned lignocellulose biomass.

    Abstract translation: 本发明涉及提高使用木素纤维素生物质作为营养源的微生物过程产量的方法。 所述方法包括用包含酚氧化酶的酶组合物调理包含木质纤维素生物质的组合物。 经调理的组合物可以在一个过程中支持更高的微生物生长速率。 在一个实施方案中，漆酶组合物用于调节源自非木本植物如玉米和甘蔗的木质纤维素生物质。 本发明还包括培养对木质纤维素生物质中抑制性化合物敏感的微生物的方法。 本发明还提供了通过在调理的木质纤维素生物质中培养生产微生物来制备产品的方法。

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