公开(公告)号：WO2012098409A1

公开(公告)日：2012-07-26

申请号：PCT/GB2012/050125

申请日：2012-01-20

Applicant: DNA ELECTRONICS LTD ,  PRODROMAKIS, Themistoklis ,  TOUMAZOU, Christofer

Inventor： PRODROMAKIS, Themistoklis ,  TOUMAZOU, Christofer

IPC: G01N27/414

CPC classification number: G01N27/4148

Abstract: A chemical sensor having a substrate, an electrode formed on or within the substrate, a chemical sensing area formed on the electrode, and coupling means on the substrate to couple the electrode to an active device arranged to receive a signal from the electrode. The active device comprises a transducer arranged to receive the signal from the electrode and provide an output signal. The signal corresponds to the concentration of ions in a sample in contact with the sensor. The sensor may be used to detect neural transmitters in a neural tissue.

Abstract translation: 具有衬底，形成在衬底上或衬底内的电极的化学传感器，形成在电极上的化学感测区域以及衬底上的耦合装置，以将电极耦合到被布置成从电极接收信号的有源器件。 有源器件包括被布置成从电极接收信号并提供输出信号的换能器。 该信号对应于与传感器接触的样品中的离子浓度。 传感器可用于检测神经组织中的神经传递器。

公开(公告)号：WO2012017424A1

公开(公告)日：2012-02-09

申请号：PCT/IB2011/053900

申请日：2011-09-06

Applicant: DNA ELECTRONICS LTD ,  PRODROKAKIS, Themistoklis ,  TOUMAZOU, Christofer

Inventor： PRODROKAKIS, Themistoklis ,  TOUMAZOU, Christofer

IPC: G01N27/12 ,  G01N27/327 ,  G01N33/543

CPC classification number: G01N27/60 ,  G01N27/127 ,  G01N27/3278 ,  G01N33/5438

Abstract: A sensor comprising a memory device having a first electrode and a first chemical- sensing layer coupled to the first electrode. The chemical-sensing layer, in the presence of an analyte, is arranged to change a property of the Memristive device. The sensor can detect an analyte by providing a sample to be detected proximate the chemical sensing layer, observing the state of the memory element; and determining a property of the sample by comparing the observed state of the memory element with a previous state. The sensor is manufactured by depositing a second electrode on a surface, depositing an active layer or layers onto said second electrode, depositing a first electrode onto said active layer(s), and coupling a chemically sensitive layer to the first electrode.

Abstract translation: 一种传感器，包括具有第一电极和耦合到第一电极的第一化学感测层的存储器件。 在分析物存在下的化学感测层被布置成改变记忆装置的性质。 传感器可以通过在化学感测层附近提供要检测的样本来检测分析物，观察存储元件的状态; 以及通过将所述存储元件的观察状态与先前状态进行比较来确定所述样本的属性。 通过在表面上沉积第二电极来制造传感器，将活性层沉积到所述第二电极上，在第一电极上沉积第一电极，以及将化学敏感层耦合到第一电极。

公开(公告)号：WO2013186537A1

公开(公告)日：2013-12-19

申请号：PCT/GB2013/051499

申请日：2013-06-07

Applicant: DNA ELECTRONICS LTD

Inventor： TOUMAZOU, Christofer ,  ALAHDAL, AbdulRahman

IPC: G11C16/04 ,  G01N27/414

CPC classification number: G01N27/4145 ,  G11C16/0416 ,  G11C16/045

Abstract: A device and method for threshold voltage programming of an Ion Sensitive Field Effect Transistor (ISFET). This is of particular use in matching ISFETs in an array, which may be used in biological reaction monitoring. A circuit comprises an Ion Sensitive Field Effect Transistor (ISFET) having a floating gate (111) and two tunnelling inputs (Vt-, 148,149; Vt+, 145, 146) coupled by capacitors (Ct-, Ct+) to the floating gate for bidirectional electron tunnelling to program a charge on the floating gate.

Abstract translation: 用于离子敏感场效应晶体管（ISFET）的阈值电压编程的装置和方法。 这特别用于匹配阵列中的ISFET，可用于生物反应监测。 电路包括具有浮置栅极（111）的离子敏感场效应晶体管（ISFET）和由电容器（Ct-，Ct +）耦合到用于双向的浮动栅极的两个隧穿输入端（Vt-，148,149; Vt +，145,146） 电子隧道来编程浮动栅极上的电荷。

公开(公告)号：WO2011110873A1

公开(公告)日：2011-09-15

申请号：PCT/GB2011/050501

申请日：2011-03-14

Applicant: DNA ELECTRONICS LTD ,  TOUMAZOU, Christofer ,  KALOFONOU, Melpomeni

Inventor： TOUMAZOU, Christofer ,  KALOFONOU, Melpomeni

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: G01N27/4145 ,  C12Q1/6825 ,  C12Q1/6827 ,  C12Q1/6858 ,  C12Q1/6869 ,  C12Q2523/125 ,  C12Q2527/119 ,  C12Q2565/607

Abstract: The use of ion sensitive field effect transistor (ISFET) to detect methylated nucleotides in a DNA sample is described. A method of detecting methylated nucleotides in a DNA sample may include the steps of treating a sample of DNA with a reagent which discriminates between methylated and non-methylated nucleotides to provide treated DNA, amplifying the treated DNA and optionally sequencing the amplified DNA. An ISFET is used to monitor the addition of one or more dNTPs in the strand extension reactions during the amplification and/or sequencing step. Suitable apparatus is also provided.

Abstract translation: 描述了使用离子敏感场效应晶体管（ISFET）来检测DNA样品中的甲基化核苷酸。 检测DNA样品中的甲基化核苷酸的方法可以包括用鉴别甲基化和非甲基化核苷酸之间的试剂处理DNA样品以提供处理的DNA，扩增经处理的DNA和任选地对扩增的DNA进行测序的步骤。 ISFET用于监测在扩增和/或测序步骤期间在链延伸反应中添加一种或多种dNTP。 还提供了合适的装置。

公开(公告)号：WO2015162301A2

公开(公告)日：2015-10-29

申请号：PCT/EP2015/059097

申请日：2015-04-27

Applicant: DNA ELECTRONICS LTD

Inventor： TOUMAZOU, Christofer ,  DAVIDSON, David ,  REED, Sam

IPC: C12Q1/68

CPC classification number: C12M1/34 ,  B01L3/5027 ,  B01L7/52 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0816 ,  B01L2300/087 ,  C12M41/48 ,  C12Q1/6825 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N27/4146 ,  C12Q2537/149 ,  C12Q2565/629 ,  C12Q2565/537 ,  C12Q2565/607

Abstract: A method of analysing nucleic acid using apparatus comprising a reaction chamber and plurality of sensors located in the base of the chamber, with each sensor preferably located within a respective well. The method comprises flowing a fluid containing the nucleic acid or fragments thereof into the reaction chamber. While the chamber is fully or at least partially sealed, amplification of the nucleic acid or said fragments is performed within the chamber using an amplification primer or primers whilst detecting the generation of amplicons using said sensors. Sequencing or hybridisation is then performed on the amplicons, and sequencing or hybridisation is detected using said sensors.

Abstract translation: 一种使用装置分析核酸的方法，所述设备包括反应室和位于所述室的底部中的多个传感器，每个传感器优选地位于相应的孔内。 该方法包括将含有核酸或其片段的流体流入反应室。 当室完全或至少部分密封时，使用扩增引物或引物在室内进行核酸或所述片段的扩增，同时使用所述传感器检测扩增子的产生。 然后对扩增子进行测序或杂交，并使用所述传感器检测测序或杂交。

公开(公告)号：WO2008107014A1

公开(公告)日：2008-09-12

申请号：PCT/EP2007/052010

申请日：2007-03-02

Applicant: DNA ELECTRONICS LTD ,  TOUMAZOU, Christofer ,  PURUSHOTHAMAN, Sunil

Inventor： TOUMAZOU, Christofer ,  PURUSHOTHAMAN, Sunil

IPC: C12Q1/68 ,  G01N27/414

CPC classification number: C12Q1/6851 ,  G01N27/4145 ,  C12Q2565/607 ,  C12Q2561/113 ,  C12Q2527/119

Abstract: Use of a pH sensor comprising an ion-sensitive field effect transistor (ISFET) to perform real time detection/ quantification of nucleic acid amplification, e.g. polymerase chain reaction (PCR) nucleic acid amplification, based on detection of protons released during the primer extension phase.

Abstract translation: 使用包含离子敏感场效应晶体管（ISFET）的pH传感器来执行核酸扩增的实时检测/定量，例如， 基于检测在引物延伸期释放的质子的聚合酶链反应（PCR）核酸扩增。

公开(公告)号：WO2013132202A1

公开(公告)日：2013-09-12

申请号：PCT/GB2012/050494

申请日：2012-03-06

Applicant: DNA ELECTRONICS LTD ,  TOUMAZOU, Christofer ,  LIU, Yan

Inventor： TOUMAZOU, Christofer ,  LIU, Yan

IPC: G01N27/414 ,  G01N27/416

CPC classification number: G01N31/16 ,  G01N27/4148

Abstract: There is provided a method and device for measuring an ion concentration of a sample. The method comprises exposing a chemical sensor to the sample to provide an electrical output signal 5 representing said ion concentration and controlling a titrator exposed to the sample to release or absorb a quantity of ions to the sample. The method may use feedback means comprising Pulse Width Modulation control to drive the titrator such that the sample maintains a stable ion concentration.

Abstract translation: 提供了用于测量样品的离子浓度的方法和装置。 该方法包括将化学传感器暴露于样品以提供代表所述离子浓度的电输出信号5并控制暴露于样品的滴定剂以释放或吸收一定量的离子到样品。 该方法可以使用包括脉宽调制控制的反馈装置来驱动滴定器，使样品保持稳定的离子浓度。

公开(公告)号：WO2012056247A1

公开(公告)日：2012-05-03

申请号：PCT/GB2011/052105

申请日：2011-10-28

Applicant: DNA ELECTRONICS LTD ,  TOUMAZOU, Christofer ,  LIU, Yan ,  GEORGIOU, Pantelis ,  OU, Chung Pei ,  REED, Sam

Inventor： TOUMAZOU, Christofer ,  LIU, Yan ,  GEORGIOU, Pantelis ,  OU, Chung Pei ,  REED, Sam

IPC: G01N27/44 ,  G01N27/414 ,  C12Q1/68

CPC classification number: C12Q1/6874 ,  C12Q1/6825 ,  C12Q1/6858 ,  G01N27/4145 ,  G01N27/44 ,  C12Q2533/101 ,  C12Q2565/301 ,  C12Q2565/607 ,  C12Q2565/501

Abstract: An apparatus comprises a transducer having a first output signal and arranged to receive an electrical input. The transducer switches the first output signal between an ON and OFF state. The apparatus comprises a chemical sensing surface coupled to the transducer arranged to receive a chemical input.A signal generator oscillates one or more of said inputs to vary the switching point of the transducer. The oscillating input may be the chemical input and/or the electrical input. The output signal may be a pulse whose period ON or OFF is determined by the oscillating input modulated by the chemical input.

Abstract translation: 一种装置包括具有第一输出信号并被布置成接收电输入的换能器。 传感器在ON和OFF状态之间切换第一个输出信号。 该装置包括耦合到换能器的化学感测表面，布置成接收化学输入。信号发生器振荡一个或多个所述输入以改变换能器的切换点。 振荡输入可以是化学输入和/或电输入。 输出信号可以是其周期ON或OFF由通过化学输入调制的振荡输入确定的脉冲。

公开(公告)号：WO2014013263A1

公开(公告)日：2014-01-23

申请号：PCT/GB2013/051930

申请日：2013-07-18

Applicant: DNA ELECTRONICS LTD

Inventor： ANSARI, Zahid ,  AMIN, Krishna ,  JORGENSEN, Ginny ,  KOLB, Kurt ,  MORLEY, Daniel ,  PATEL, Alpesh ,  REED, Sam ,  SHEPHERD, Leila ,  TOUMAZOU, Christofer

IPC: C12Q1/68

CPC classification number: C12Q1/6869 ,  C12Q1/6874 ,  C12Q2527/101 ,  C12Q2527/119 ,  C12Q2535/122 ,  C12Q2543/101 ,  C12Q2547/101 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: Provided is a sensing apparatus comprising a chip for integrated amplification and sequencing of a template polynucleotide in a sample. The apparatus comprises a chip with at least one ISFET in a well or chamber, amplification means for amplifying the template polynucleotide on a surface of said chip and comprising at least one heating means suitable for conducting amplification of the template polynucleotide at temperatures elevated with respect to room temperature, and sequencing means for sequencing the amplified template polynucleotide in said well or chamber. Methods of use are also provided.

Abstract translation: 提供了一种感测装置，包括用于样本中模板多核苷酸的集成扩增和测序的芯片。 该装置包括在井或室中具有至少一个ISFET的芯片，用于在所述芯片的表面上扩增模板多核苷酸的扩增装置，并且包含至少一种适于在相对于 以及用于测序所述孔或室中扩增的模板多核苷酸的测序装置。 还提供了使用方法。

公开(公告)号：WO2011095793A1

公开(公告)日：2011-08-11

申请号：PCT/GB2011/050133

申请日：2011-01-28

Applicant: DNA ELECTRONICS LTD ,  GEORGIOU, Pantelis ,  PRODROKAKIS, Themistoklis ,  CONSTANDINOU, Timothy G ,  TOUMAZOU, Christofer

Inventor： GEORGIOU, Pantelis ,  PRODROKAKIS, Themistoklis ,  CONSTANDINOU, Timothy G ,  TOUMAZOU, Christofer

IPC: G01N27/414 ,  G01N33/487 ,  B01L3/00

CPC classification number: G01N27/4163 ,  G01N27/4145 ,  G01N33/4836

Abstract: A sensor system comprising a substrate and integrated onto the substrate an array (7) of sensor elements (1), each sensor element comprising one or more inductors (3), one or more electrochemical sensors (4), and one or more optical sensors (2). The system further comprising a controller configured in use to separately address each of the sensor elements (1) to drive the respective inductors and receive outputs of the respective sensors.

Abstract translation: 一种传感器系统，包括基板并将传感器元件（1）的阵列（7）集成到所述基板上，每个传感器元件包括一个或多个电感器（3），一个或多个电化学传感器（4）和一个或多个光学传感器 （2）。 所述系统还包括控制器，其被配置为用于单独地寻址每个所述传感器元件（1）以驱动相应的电感器并接收各个传感器的输出。