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    INTEGRATION OF ELECTROCHEMICAL PH MODULATION WITH LAB ON A CHIP TECHNOLOGIES
    2.
    发明申请
    INTEGRATION OF ELECTROCHEMICAL PH MODULATION WITH LAB ON A CHIP TECHNOLOGIES 审中-公开
    电化学PH调制与实验室芯片技术的集成

    公开(公告)号：WO2017077384A1

    公开(公告)日：2017-05-11

    申请号：PCT/IB2016/001691

    申请日：2016-11-04

    Applicant: ROBERT BOSCH GMBH

    Inventor： FOMINA, Nadezda ,  JOHNSON, Christopher ,  AHMAD, Habib ,  LANG, Christoph ,  LAERMER, Franz

    IPC: B01L3/00 ,  G01N27/416 ,  C12N15/10 ,  C12Q1/68

    CPC classification number: B01L3/5027 ,  B01L2300/087 ,  B01L2400/046 ,  C12N15/1003 ,  C12Q1/6825 ,  G01N27/3277 ,  C12Q2527/119 ,  C12Q2565/607

    Abstract: At least one electrode is integrated on a lab on a chip cartridge in a sample preparation chamber of the cartridge, a DNA hybridization chamber of the cartridge, a protein assay chamber of the cartridge, and/or a detection chamber of the cartridge, for example, where the electrode is used to generate pH electrochemically in order to activate, deactivate, or intermediately attenuate an enzyme's activity on demand, in order to increase the fidelity of analyte detection, for cell lysis, for protein extraction, for DNA dehybridization, for primer hybridization control, for sample pre concentration, and/or for washing to remove non target species.

    Abstract translation: 将至少一个电极集成在盒的样品制备室中的芯片盒上的实验室，盒的DNA杂交室，盒的蛋白质测定室和/或 例如，在电极用于电化学生成pH以激活，失活或中间减弱酶的活性时，为了提高分析物检测，细胞裂解，蛋白质的保真度 提取，用于DNA去杂交，用于引物杂交对照，用于样品预浓缩，和/或用于洗涤以去除非目标物质。

    DEVICES AND KITS FOR MEASURING BIOLOGICAL RESULTS
    5.
    发明申请
    DEVICES AND KITS FOR MEASURING BIOLOGICAL RESULTS 审中-公开
    用于测量生物学结果的装置和工具

    公开(公告)号：WO2015170190A2

    公开(公告)日：2015-11-12

    申请号：PCT/IB2015/001424

    申请日：2015-04-10

    Applicant: CREDO BIOMEDICAL PTE LTD.

    Inventor： OU, Chung-pei ,  RAHMAN, Abdur Rub, Abdur ,  SAGAR, Kaushal ,  KAO, Stephen, Chang-chi ,  WINSTON WONG, Jr.

    CPC classification number: G01N33/52 ,  C12Q1/6813 ,  G01N33/84 ,  C12Q2527/119 ,  C12Q2563/173

    Abstract: The present invention relates to a kit and a device for measuring nucleic acid amplification through colour differentiation wherein said kit contains at least one pH indicator dye, one or more contained amplification reagents. The kit and device of the present invention also are used to detect, measure and/or record enzymatic reactions that result in pH changes. The kit and device provide a mechanism to detect pH change by utilizing a pH indicator dye, thus making it observable with the un-aided eye. The kit contains a device for carrying out said reactions. The device contains at least one container, reagents, a pH indicator, a heating or cooling means where needed and a magnetic component.

    Abstract translation: 本发明涉及用于通过颜色分化测量核酸扩增的试剂盒和装置，其中所述试剂盒含有至少一种pH指示剂染料，一种或多种含有扩增试剂。 本发明的试剂盒和装置还用于检测，测量和/或记录导致pH变化的酶反应。 试剂盒和装置提供了通过使用pH指示剂染料来检测pH变化的机制，从而使其可以用无辅助眼睛观察到。 试剂盒包含用于进行所述反应的装置。 该装置包含至少一个容器，试剂，pH指示剂，需要的加热或冷却装置和磁性组分。

    NUCLEIC ACID DETECTION METHOD AND KIT
    6.
    发明申请
    NUCLEIC ACID DETECTION METHOD AND KIT 审中-公开
    核酸检测方法和试剂盒

    公开(公告)号：WO2015095929A1

    公开(公告)日：2015-07-02

    申请号：PCT/AU2014/050443

    申请日：2014-12-23

    Applicant: THE UNIVERSITY OF QUEENSLAND

    Inventor： TRAU, Matt ,  WEE, Eugene ,  BOTELLA, Jose Ramon

    IPC: C12Q1/68 ,  G01N33/50

    CPC classification number: C12Q1/6851 ,  C12Q1/6844 ,  C12Q1/689 ,  C12Q1/6893 ,  C12Q1/703 ,  C12Q1/708 ,  C12Q2527/119 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2565/113

    Abstract: A method and kit for detecting a target nucleic acid is provided wherein combining an amplified nucleic acid with a particle such as a paramagnetic bead form a flocculated complex which can be detected by visual inspection. The volume of nucleic acid sample that can be detected is as low as a few microlitres. The method can be applied to in-the-field or point-of-care diagnosis for a rapid determination of the presence or absence of the target nucleic acid. The methylation status of a target nucleic acid can also be determined. The method and lit may have general applicability to detecting diseases in plants and animals, environmental testing and testing for contamination of foods and other edible products.

    Abstract translation: 提供了用于检测靶核酸的方法和试剂盒，其中将扩增的核酸与诸如顺磁珠的颗粒组合形成可以通过目视检测的絮凝复合物。 可以检测到的核酸样品的体积低至几微升。 该方法可以应用于现场或点护理诊断以快速测定靶核酸的存在或不存在。 也可以确定靶核酸的甲基化状态。 该方法和照明可能具有普遍适用于检测植物和动物疾病，环境测试和食品和其他可食用产品污染的检测。

    SYSTEM AND METHOD FOR OPERATION OF ISFET ARRAYS USING pH INERT REFERENCE SENSORS
    8.
    发明申请
    SYSTEM AND METHOD FOR OPERATION OF ISFET ARRAYS USING pH INERT REFERENCE SENSORS 审中-公开
    使用pH INERT参考传感器操作ISFET阵列的系统和方法

    公开(公告)号：WO2014037423A1

    公开(公告)日：2014-03-13

    申请号：PCT/EP2013/068320

    申请日：2013-09-05

    Applicant: ROCHE DIAGNOSTICS GMBH ,  F. HOFFMANN-LA ROCHE AG

    Inventor： WONG, Chiu Tai Andrew ,  FERRERI, Gianni Calogero ,  BASURAY, Sagnik ,  BAPPAL, Arthika

    IPC: C12Q1/68

    CPC classification number: C12Q1/6874 ,  C12Q1/6869 ,  C12Q2527/119 ,  C12Q2535/101 ,  C12Q2535/122 ,  C12Q2545/113 ,  C12Q2563/137 ,  C12Q2563/149 ,  C12Q2565/607 ,  C12Q2565/629 ,  H01L27/105

    Abstract: An embodiment of a method for sequencing a species of nucleic acid template using pH inert reference sensors is described that comprises the steps of: introducing a nucleotide species to an array of wells where a plurality of the wells comprise a species of nucleic acid template and a plurality of the wells comprise a plurality of functional groups with a high pH buffering characteristic, and in at least a first well a polymerase species incorporates the nucleotide species into a plurality of strands complementary to the species of nucleic acid template disposed in the first well and results in a release of a plurality of hydrogen ions; detecting a signal in the first well that is responsive to the hydrogen ions and one or more noise sources; detecting a signal in a second well comprising the functional groups with the high pH buffering characteristic that is responsive to the one or more noise sources; and subtracting the second well signal from the first well signal to generate a corrected signal associated with the detected hydrogen ions.

    Abstract translation: 描述了使用pH惰性参考传感器对核酸模板种类进行测序的方法的实施方案，其包括以下步骤：将核苷酸物种引入孔阵列，其中多个孔包含核酸模板和 多个孔包括具有高pH缓冲特征的多个官能团，并且在至少第一阱中，聚合酶物种将核苷酸物种并入与设置在第一孔中的核酸模板的种类互补的多个链， 导致多个氢离子的释放; 检测第一阱中响应于氢离子和一个或多个噪声源的信号; 检测包含响应于所述一个或多个噪声源的高pH缓冲特性的功能组的第二阱中的信号; 以及从第一阱信号中减去第二阱信号以产生与检测到的氢离子相关联的校正信号。

    METHOD OF PERFORMING DIGITAL PCR
    9.
    发明申请
    METHOD OF PERFORMING DIGITAL PCR 审中-公开
    执行数字PCR的方法

    公开(公告)号：WO2013158982A1

    公开(公告)日：2013-10-24

    申请号：PCT/US2013/037352

    申请日：2013-04-19

    Applicant: LIFE TECHNOLOGIES CORPORATION

    Inventor： CHEN, Caifu ,  McFARLAND, Casey, R. ,  KEYS, Jr., David, N.

    IPC: C12Q1/68

    CPC classification number: C12Q1/686 ,  C12Q1/6851 ,  C12Q1/6858 ,  C12Q2527/119 ,  C12Q2563/159 ,  C12Q2565/607 ,  C12Q2565/301

    Abstract: A method of detection of a target nucleic acid is provided. The method includes fractionating a sample into a plurality of sample volumes wherein more than 50% of the fractions contain no more than 1 target nucleic acid molecule per sample volumes, and subjecting the plurality of sample volumes to conditions for amplification. The method further includes detecting a change in ion concentration in a sample volume wherein a target nucleic acid is present, counting the number of fractions with an amplified target nucleic acid, and determining the quantity of target nucleic acid in the sample.

    Abstract translation: 提供了检测靶核酸的方法。 该方法包括将样品分馏成多个样品体积，其中超过50％的级分每个样品体积含有不超过1个靶核酸分子，并且对多个样品体积进行扩增条件。 该方法还包括检测样品体积中的离子浓度的变化，其中存在靶核酸，用扩增的靶核酸计数分数，并测定样品中靶核酸的量。

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