公开(公告)号：WO2011150232A2

公开(公告)日：2011-12-01

申请号：PCT/US2011/038180

申请日：2011-05-26

Applicant: INTEGENX INC. ,  MAJLOF, Lars ,  VAN GELDER, Ezra

Inventor： MAJLOF, Lars ,  VAN GELDER, Ezra

IPC: G01N27/447

CPC classification number: G01N27/44708

Abstract: This invention provides a capillary electrophoresis device in which capillaries are thermally regulated on a thermally responsive electrical path attached to an electrically insulating circuit board. This invention also provides an optical scanner useful for scanning an array of capillaries. A laser, optical detector and optical selector are in an arrangement that allows the optical detector to selectively detect an optical signal from any one or more of the plurality of electrophoresis capillaries.

Abstract translation: 本发明提供一种毛细管电泳装置，其中毛细管在附着于电绝缘电路板的热响应电路上进行热调节。 本发明还提供了一种用于扫描毛细管阵列的光学扫描仪。 激光器，光学检测器和光学选择器处于允许光学检测器选择性地检测来自多个电泳毛细管中的一个或多个的光学信号的布置。

公开(公告)号：WO2013130910A1

公开(公告)日：2013-09-06

申请号：PCT/US2013/028462

申请日：2013-02-28

Applicant: INTEGENX INC. ,  EBERHART, David ,  LI, Yuan ,  OGG, James ,  VAN GELDER, Ezra ,  WILLIAMS, Stephen, J. ,  WOUDENBERG, Timothy ,  BURGI, Dean, S. ,  NIELSEN, William, D.

Inventor： EBERHART, David ,  LI, Yuan ,  OGG, James ,  VAN GELDER, Ezra ,  WILLIAMS, Stephen, J. ,  WOUDENBERG, Timothy ,  BURGI, Dean, S. ,  NIELSEN, William, D.

IPC: C12Q1/68

CPC classification number: C12Q1/68 ,  B01L3/502715 ,  B01L7/52 ,  B01L2200/04 ,  B01L2200/0684 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2300/1805 ,  B01L2300/1822 ,  B01L2300/1838 ,  B01L2400/0478 ,  B01L2400/0616 ,  B01L2400/0655 ,  B01L2400/0683 ,  C12Q1/686 ,  C12Q2565/629

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种集成且自动化的样本到回答系统，其从包含生物材料的样本开始，在不到两个小时内生成遗传图谱。 在某些实施方案中，生物材料是DNA并且遗传谱涉及确定受试者的一个或多个基因座（例如遗传基因座）处的等位基因，例如STR（短串联重复序列）分布，例如如在 CODIS系统。 该系统可以执行多种操作，包括（a）提取和分离核酸; （b）扩增选定基因座的核苷酸序列（例如遗传基因座）; 和（c）扩增产物的检测和分析。 这些操作可以在包含几个集成模块的系统中执行，包括分析物准备模块; 一个检测和分析模块以及一个控制模块。

公开(公告)号：WO2012024658A2

公开(公告)日：2012-02-23

申请号：PCT/US2011/048528

申请日：2011-08-20

Applicant: INTEGENX, INC. ,  JOVANOVICH, Stevan, B. ,  STERN, Seth ,  VAN GELDER, Ezra ,  VANGBO, Mattias

Inventor： JOVANOVICH, Stevan, B. ,  STERN, Seth ,  VAN GELDER, Ezra ,  VANGBO, Mattias

IPC: C12M1/34

CPC classification number: B01J19/0046 ,  B01J2219/00389 ,  B01J2219/00599 ,  B01J2219/00716 ,  B01J2219/00722 ,  B01L3/502738 ,  B01L7/52 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2200/16 ,  B01L2300/0816 ,  B01L2300/0867 ,  B01L2300/087 ,  B01L2400/043 ,  B01L2400/0481 ,  B01L2400/0655 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6874 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/0084

Abstract: The invention provides systems, devices, methods, and kits for performing an integrated analysis. The integrated analysis can include sample processing, library construction, amplification, and sequencing. The integrated analysis can be performed within one or more modules that are fluidically connected to each other. The one or more modules can be controlled and/or automated by a computer. The integrated analysis can be performed on a tissue sample, a clinical sample, or an environmental sample. The integrated analysis system can have a compact format and return results within a designated period of time.

Abstract translation: 本发明提供用于执行综合分析的系统，设备，方法和套件。 综合分析可以包括样品处理，文库构建，扩增和测序。 综合分析可以在彼此流体连接的一个或多个模块内执行。 一个或多个模块可以由计算机控制和/或自动化。 综合分析可以在组织样本，临床样本或环境样本上进行。 综合分析系统可以有一个紧凑的格式，并在指定的时间内返回结果。

公开(公告)号：WO2013059750A1

公开(公告)日：2013-04-25

申请号：PCT/US2012/061223

申请日：2012-10-20

Applicant: INTEGENX INC. ,  NIELSEN, Willian, D. ,  BELCINSKI, Richard, J. ,  BOGDAN, Gregory ,  EBERHART, David ,  EL-SISSI, Omar ,  JOVANOVICH, Stevan, B. ,  RECKNOR, Michael ,  VAN GELDER, Ezra ,  WYRICK, David, W.

Inventor： NIELSEN, Willian, D. ,  BELCINSKI, Richard, J. ,  BOGDAN, Gregory ,  EBERHART, David ,  EL-SISSI, Omar ,  JOVANOVICH, Stevan, B. ,  RECKNOR, Michael ,  VAN GELDER, Ezra ,  WYRICK, David, W.

IPC: C12M1/00

CPC classification number: C12Q1/68 ,  B01L3/502715 ,  B01L3/5029 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/028 ,  B01L2200/04 ,  B01L2200/0647 ,  B01L2200/143 ,  B01L2300/023 ,  B01L2300/027 ,  B01L2300/0672 ,  B01L2300/123 ,  B01L2400/043 ,  B01L2400/0478 ,  B01L2400/0616 ,  G01N27/44704 ,  G01N2035/00158

Abstract: This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module.

Abstract translation: 本公开提供了一种综合和自动化的样本回答系统，其从包含生物材料的样品开始，在少于两小时内产生遗传图谱。 在某些实施方案中，生物材料是DNA，并且遗传图谱涉及确定受试者的一个或多个位点（例如，遗传基因座）等位基因，例如STR（短串联重复）分布，例如如 CODIS系统。 该系统可以执行若干操作，包括（a）提取和分离核酸; （b）在选择的位点（例如遗传基因座）扩增核苷酸序列; 和（c）扩增产物的检测和分析。 这些操作可以在包括多个集成模块的系统中进行，包括分析物准备模块; 检测和分析模块和控制模块。

公开(公告)号：WO2012097233A1

公开(公告)日：2012-07-19

申请号：PCT/US2012/021217

申请日：2012-01-13

Applicant: INTEGENX INC. ,  EBERHART, David ,  VAN GELDER, Ezra

Inventor： EBERHART, David ,  VAN GELDER, Ezra

IPC: B81B3/00

CPC classification number: F16K99/0015 ,  F16K99/0026 ,  F16K99/0059

Abstract: This invention provides a device comprising at least one diaphragm valve actuated by a hydraulic actuation system. The device is comprised in a combination that includes a fluidics layer, an actuation layer and an elastic layer sandwiched between the fluidics layer and the actuation layer, wherein the diaphragm valve comprises: (a) a valve inlet and a valve outlet comprised in the fluidics layer; (b) a valve seat; (c) a diaphragm comprised in the elastic layer, wherein the diaphragm is actuatable to move into contact or out of contact with the valve seat, thereby closing or opening the diaphragm valve; and (d) an actuator comprising: (1 ) a hydraulic conduit comprised at least in part in the actuation layer; (2) a translator; and (3) an incompressible fluid contained within the hydraulic conduit, wherein the incompressible fluid communicates with the translator and with the diaphragm.

Abstract translation: 本发明提供了一种包括由液压致动系统致动的至少一个隔膜阀的装置。 该装置包括流体层，致动层和夹在流体层和致动层之间的弹性层的组合，其中隔膜阀包括：（a）阀入口和包括在流体中的阀出口 层; （b）阀座; （c）包括在所述弹性层中的隔膜，其中所述隔膜可致动以与阀座接触或脱离接触，从而关闭或打开隔膜阀; 和（d）致动器，包括：（1）至少部分地包括在所述致动层中的液压管道; （2）翻译; 和（3）包含在液压管道内的不可压缩流体，其中不可压缩流体与平移器和隔膜连通。

公开(公告)号：WO2011123801A1

公开(公告)日：2011-10-06

申请号：PCT/US2011/030973

申请日：2011-04-01

Applicant: INTEGENX INC. ,  EBERHART, David ,  VAN GELDER, Ezra

Inventor： EBERHART, David ,  VAN GELDER, Ezra

IPC: G01N15/06

CPC classification number: F16K99/0015 ,  B01J2219/00353 ,  B01J2219/00389 ,  B01J2219/00398 ,  B01J2219/00722 ,  B01L3/502707 ,  B01L3/502738 ,  B01L2300/0816 ,  B01L2300/0861 ,  B01L2300/123 ,  B01L2300/165 ,  B01L2400/0655 ,  C40B50/08 ,  C40B60/14 ,  F16K99/0059 ,  Y10T137/0318 ,  Y10T137/8593 ,  Y10T428/13 ,  Y10T428/1397

Abstract: In one aspect, this invention provides an article fabricated in one piece comprising at least one aperture through the piece, wherein the aperture defines a non-micro fiuidic volume, and a micro fiuidic channel formed in a surface of the piece onto which the aperture opens, wherein the channel is in fiuidic communication with the aperture, wherein the aperture and the microfluidic channel define a fiuidic circuit.

Abstract translation: 在一个方面，本发明提供了一件制造的制品，其包括通过该片的至少一个孔，其中孔限定非微小流体体积，以及形成在该孔的表面上的微流体通道，孔开口 ，其中所述通道与所述孔流体通信，其中所述孔和所述微流体通道限定流体电路。

公开(公告)号：WO2011068762A1

公开(公告)日：2011-06-09

申请号：PCT/US2010/058227

申请日：2010-11-29

Applicant: INTEGENX INC. ,  KOBRIN, Boris ,  BLAGA, Iuliu, I. ,  NIELSEN, William ,  QI, Shize, D. ,  VAN GELDER, Ezra

Inventor： KOBRIN, Boris ,  BLAGA, Iuliu, I. ,  NIELSEN, William ,  QI, Shize, D. ,  VAN GELDER, Ezra

IPC: B23B7/00

CPC classification number: F16K99/0001 ,  B01F11/0071 ,  B01F13/0059 ,  B32B7/02 ,  B32B9/04 ,  B33Y80/00 ,  F16K99/0015 ,  F16K99/0059 ,  F16K2099/008 ,  F16K2099/0084 ,  F16K2099/0094 ,  Y10T137/0318 ,  Y10T137/2213 ,  Y10T137/86453 ,  Y10T137/87249 ,  Y10T156/1039 ,  Y10T428/31504

Abstract: This invention provides composite plastic articles and methods of making them. The articles can be fluidic or microfluidic devices having fluidic conduits and, optionally, pneumatic conduits that regulate flow in the fluidic conduits. The articles comprise a first substrate coated with a layer of a material that comprises, or onto which have been introduced, reactive groups. For example, the substrate can be a plastic coated with an oxide or a siloxane onto which hydroxyl groups have been introduced. These articles are covalently bonded with other articles comprising reactive groups on their surfaces, for example, polysiloxanes treated to have silanol groups. Certain articles have specified locations on their surfaces that are not bonded to the other piece. For example, the coating can be removed from these locations before bonding. Such locations can be useful as functional elements of various devices, such as valve seats in valves of microfluidic devices.

Abstract translation: 本发明提供复合塑料制品及其制造方法。 制品可以是具有流体导管的流体或微流体装置，以及可选地调节流体管道中的流动的气动导管。 制品包括涂覆有材料层的第一基材，其包含或已经引入反应性基团的材料层。 例如，基底可以是涂覆有已经引入羟基的氧化物或硅氧烷的塑料。 这些制品与其表面上包含反应性基团的其它制品共价键合，例如被处理为具有硅烷醇基团的聚硅氧烷。 某些物品在其表面上指定了未粘结到另一件的位置。 例如，可以在粘合之前从这些位置去除涂层。 这样的位置可以用作各种装置的功能元件，例如微流体装置的阀中的阀座。

公开(公告)号：WO2010141921A1

公开(公告)日：2010-12-09

申请号：PCT/US2010/037545

申请日：2010-06-04

Applicant: INTEGENX INC. ,  JOVANOVICH, Stevan, B. ,  NIELSEN, William, D. ,  COHEN, David, S. ,  RECKNOR, Michael ,  VANGBO, Mattias ,  VAN GELDER, Ezra ,  MAJLOF, Lars ,  EL-SISSI, Omar

Inventor： JOVANOVICH, Stevan, B. ,  NIELSEN, William, D. ,  COHEN, David, S. ,  RECKNOR, Michael ,  VANGBO, Mattias ,  VAN GELDER, Ezra ,  MAJLOF, Lars ,  EL-SISSI, Omar

IPC: C02F1/40

CPC classification number: C12Q1/6806 ,  G01J3/4406 ,  G01N1/31 ,  G01N1/405 ,  G01N21/64 ,  G01N27/44721 ,  G01N27/44791 ,  G01N27/745 ,  G01N35/00029 ,  G01N2035/00148 ,  G01N2035/00247 ,  G01N2201/06113 ,  G01N2201/062 ,  Y10T436/11 ,  Y10T436/143333 ,  Y10T436/25 ,  Y10T436/2575

Abstract: The invention provides a system that can process a raw biological sample, perform a biochemical reaction and provide an analysis readout. For example, the system can extract DNA from a swab, amplify STR loci from the DNA, and analyze the amplified loci and STR markers in the sample. The system integrates these functions by using microfluidic components to connect what can be macrofluidic functions. In one embodiment the system includes a sample purification module, a reaction module, a post- reaction clean-up module, a capillary electrophoresis module and a computer. In certain embodiments, the system includes a disposable cartridge for performing analyte capture. The cartridge can comprise a fluidic manifold having macrofluidic chambers mated with microfluidic chips that route the liquids between chambers. The system fits within an enclosure of no more than 10 ft 3 , and can be a closed, portable, and/or a battery operated system. The system can be used to go from raw sample to analysis in less than 4 hours.

Abstract translation: 本发明提供一种可以处理原始生物样品，进行生化反应并提供分析读数的系统。 例如，该系统可以从拭子中提取DNA，从DNA扩增STR基因座，并分析样品中扩增的位点和STR标记。 该系统通过使用微流体组件来集成这些功能，以连接什么是宏流动功能。 在一个实施方案中，该系统包括样品纯化模块，反应模块，反应后清洗模块，毛细管电泳模块和计算机。 在某些实施例中，该系统包括用于执行分析物捕获的一次性盒。 墨盒可以包括流体歧管，该流体歧管具有与微流体芯片配合的大流动腔室，所述微流体芯片在腔室之间引导液体。 该系统适用于不超过10英尺3的外壳，可以是封闭的，便携式的和/或电池供电的系统。 该系统可用于在不到4小时内从原始样品进行分析。

公开(公告)号：WO2013049071A1

公开(公告)日：2013-04-04

申请号：PCT/US2012/057130

申请日：2012-09-25

Applicant: INTEGENX INC. ,  VAN GELDER, Ezra

Inventor： VAN GELDER, Ezra

IPC: B01L3/02

CPC classification number: B01L9/547 ,  B01L3/0279 ,  B01L2200/025 ,  B01L2200/148 ,  B01L2300/0838 ,  G01N35/1009 ,  G01N35/1065 ,  G01N2035/1037 ,  G01N2035/1076 ,  Y10T137/0402 ,  Y10T137/598

Abstract: One aspect of the technology relates to a method comprising the steps of: moving a frame movably holding at least one hollow bore pin having a liquid dispensing tip, such that the liquid dispensing tip of the at least one hollow bore pin moves into a respective well of a set of target wells, and the liquid dispensing tip makes contact with a bottom surface of the respective well; continuing to move the frame after the liquid dispensing tip makes contact, such that the at least one hollow bore pin moves to a particular position (e.g., vertical position) within a range of positions (e.g., vertical positions) relative to and permitted by the frame.

Abstract translation: 该技术的一个方面涉及一种方法，包括以下步骤：移动可移动地保持具有液体分配尖端的至少一个中空孔销的框架，使得至少一个中空孔销的液体分配尖端移动到相应的孔 的一组目标井，并且液体分配尖端与相应井的底表面接触; 在液体分配尖端接触之后继续移动框架，使得至少一个中空孔销相对于和允许的位置（例如，垂直位置）的范围内移动到特定位置（例如，垂直位置） 帧。

公开(公告)号：WO2013028643A1

公开(公告)日：2013-02-28

申请号：PCT/US2012/051632

申请日：2012-08-20

Applicant: INTEGENX INC. ,  TANNER, Michael ,  JOVANOVICH, Stevan, B. ,  VAN GELDER, Ezra ,  HARRIS, Dennis ,  PARK, Charles ,  STERN, Seth

Inventor： TANNER, Michael ,  JOVANOVICH, Stevan, B. ,  VAN GELDER, Ezra ,  HARRIS, Dennis ,  PARK, Charles ,  STERN, Seth

IPC: C07H21/04 ,  C40B30/04

CPC classification number: C07C49/753 ,  C12Q1/6855 ,  C12Q2521/507 ,  C12Q2535/122 ,  C12Q2565/543

Abstract: The present invention provides methods and devices for performing nucleic acid amplification and sequencing on a solid substrate (e.g., a flow cell), including preparation of libraries of amplified DNA fragments for massively parallel (next-generation) sequencing.

Abstract translation: 本发明提供了用于在固体基质（例如流动细胞）上进行核酸扩增和测序的方法和装置，包括用于大规模并行（下一代）扩增的DNA片段文库的制备， 测序