公开(公告)号：WO2007064404A3

公开(公告)日：2007-06-07

申请号：PCT/US2006/039135

申请日：2006-10-03

Applicant: KIONIX, INC. ,  YOUNG, Lincoln ,  ZHOU, Peng

Inventor： YOUNG, Lincoln ,  ZHOU, Peng

IPC: B01L3/00 ,  F04B43/04 ,  B32B27/00 ,  F04B53/10

Abstract: Plastic microfluidic structures having a substantially rigid diaphragm that actuates between a relaxed state wherein the diaphragm sits against the surface of a substrate and an actuated state wherein the diaphragm is moved away from the substrate. As will be seen from the following description, the microfluidic structures formed with this diaphragm provide easy to manufacture and robust systems, as well readily made components such as valves and pumps.

公开(公告)号：WO2009049268A1

公开(公告)日：2009-04-16

申请号：PCT/US2008/079659

申请日：2008-10-10

Applicant: KIONIX, INC. ,  ZHOU, Peng ,  YOUNG, Lincoln C. ,  ROSWECH, Todd ,  SPIZZ, Gwendolyn ,  CHEN, Zongyuan ,  THOMAS, Benjamin W. ,  LEE, Travis

Inventor： ZHOU, Peng ,  YOUNG, Lincoln C. ,  ROSWECH, Todd ,  SPIZZ, Gwendolyn ,  CHEN, Zongyuan ,  THOMAS, Benjamin W. ,  LEE, Travis

IPC: B01L3/00

CPC classification number: B01F13/0059 ,  B01F5/0683 ,  B01F5/0688 ,  B01F11/0074 ,  B01L3/50273 ,  B01L7/52 ,  B01L2200/027 ,  B01L2200/0621 ,  B01L2200/10 ,  B01L2300/0816 ,  B01L2300/1816 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/1844 ,  B01L2300/185 ,  B01L2400/0487 ,  B01L2400/0638 ,  B01L2400/084

Abstract: A microf luidic device for analyzing a sample of interest is provided. The tnicrof luidic device can comprise a microfluidic device body, wherein the microf luidic device body comprises a sample preparation area (101), a nucleic acid amplification area (102), a nucleic acid analysis area (103), and a network of fluid channels. Each of the sample preparation area (101), the nucleic acid amplification area (102) and the nucleic acid analysis area (103) are fluidly interconnected to at least one of the other two areas by at least one of the fluid channels. Using the microfluidic device, sample preparation can be combined with amplification of a biologically active molecule, and a suitable biological sample can be provided for analysis and/or detection of a molecule of interest. The small-scale apparatus and methods provided are easier, faster, less expensive, and equally efficacious compared to larger scale equipment for the preparation and analysisof a biological sample.

Abstract translation: 提供了一种用于分析感兴趣样品的微流控装置。 微流体装置可以包括微流体装置主体，其中微流体装置主体包括样品制备区域（101），核酸扩增区域（102），核酸分析区域（103）和流体通道网络 。 每个样品制备区域（101），核酸扩增区域（102）和核酸分析区域（103）通过至少一个流体通道流体地互连到另外两个区域中的至少一个区域。 使用微流体装置，样品制备可以与生物活性分子的扩增相结合，并且可以提供合适的生物样品用于分析和/或检测目的分子。 与用于生物样品的制备和分析的较大规模的设备相比，所提供的小型装置和方法更容易，更快，更便宜，并且同样有效。

公开(公告)号：WO2004103562A2

公开(公告)日：2004-12-02

申请号：PCT/US2004/014811

申请日：2004-05-12

Applicant: KIONIX, Inc. ,  ZHOU, Peng ,  YOUNG, Lincoln

Inventor： ZHOU, Peng ,  YOUNG, Lincoln

IPC: B01L

CPC classification number: B01L3/502715 ,  B01J2219/0081 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/027 ,  B01L2200/028 ,  B01L2300/043 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2300/0874 ,  G01N30/6082 ,  G01N30/6095 ,  Y10T29/49 ,  Y10T29/49002 ,  Y10T436/11 ,  Y10T436/117497 ,  Y10T436/118339 ,  G01N30/74 ,  G01N30/6047 ,  G01N2030/8881

Abstract: A reconfigurable modular microfluidic system, providing a microfluidic breadboard platform for the formation of fluidic network and fluidic sealing upon a system assembly. Modular microfluidic elements or "chips" are arranged on a precisely machined alignment base to form a fluidic network, with fluid connections provided directly from chip-to-chip at overlapping corners. Fluidic access to external devices is possible at every fluid connection and through special ingress/egress chips. By maintaining a largely planar layout, optical access is provided for detecting or visualization for every chip. The assembly may be covered by a perforated cover plate.

Abstract translation: 可重构模块化微流体系统，提供用于在系统组件上形成流体网络和流体密封的微流体面包板平台。 模块化微流体元件或“芯片”被布置在精确加工的对准基底上以形成流体网络，其中在重叠的角落处直接从芯片到芯片提供流体连接。 在每个流体连接和特殊的入口/出口芯片处都可以对外部设备进行流体访问。 通过维持大体平面的布局，提供了光学访问，用于每个芯片的检测或可视化。 组件可以被穿孔盖板覆盖。

公开(公告)号：WO2006043922A1

公开(公告)日：2006-04-27

申请号：PCT/US2004/033791

申请日：2004-10-13

Applicant: KIONIX, INC. ,  ZHOU, Peng ,  YOUNG, Lincoln, C.

Inventor： ZHOU, Peng ,  YOUNG, Lincoln, C.

IPC: C09J5/10

CPC classification number: B29C66/54 ,  B01L3/502707 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/0816 ,  B01L2300/0887 ,  B29C65/4815 ,  B29C65/483 ,  B29C65/4895 ,  B29C66/543 ,  B29C66/71 ,  B29L2031/756 ,  B29K2069/00 ,  B29K2033/08 ,  B29K2025/06

Abstract: A method for making a polymeric microfluidic structure in which two or more components (layers) of the microfluidic structure are fixedly bonded or laminated with a weak solvent bonding agent, particularly acetonitrile or a mixture of acetonitrile and alcohol. In an aspect, acetonitrile can be used as a weak solvent bonding agent to enclose a microstructure fabricated in or on a non-elastomeric polymer such as polystyrene, polycarbonate, acrylic or other linear polymer to form a three-dimensional microfluidic network. The method involves the steps of wetting at least one of the opposing surfaces of the polymeric substrate components with the weak solvent bonding agent in a given, lower temperature range, adjacently contacting the opposing surfaces, and thermally activating the bonding agent at a higher temperature than the lower temperature range for a given period of time. The contacted polymeric substrates may also be aligned prior to thermal activation and compressed during thermal activation. A laminated, polymeric microfluidic structure is also disclosed.

Abstract translation: 一种制备聚合物微流体结构的方法，其中微流体结构的两个或多个组分（层）用弱溶剂粘合剂，特别是乙腈或乙腈和醇的混合物固定粘合或层压。 在一个方面，乙腈可以用作弱溶剂粘合剂以包围在非弹性聚合物如聚苯乙烯，聚碳酸酯，丙烯酸或其它线性聚合物中或其上制造的微结构，以形成三维微流体网络。 该方法包括以下步骤：在给定的较低温度范围内用弱溶剂粘合剂润湿聚合物基材组分的至少一个相对表面，相邻地接触相对表面，并在比例 在一定时间内较低的温度范围。 接触的聚合物基材还可以在热活化之前对准，并在热活化期间被压缩。 还公开了层压聚合物微流体结构。

公开(公告)号：WO2003103836A1

公开(公告)日：2003-12-18

申请号：PCT/US2003/015191

申请日：2003-05-13

Applicant: KIONIX, INC.

Inventor： YOUNG, Lincoln C. ,  ZHOU, Peng ,  PINNISI, Michael D. ,  MARUSAK, Ronald E. ,  MOON, James E.

IPC: B01L3/00

CPC classification number: B01L3/502776 ,  B01D11/0415 ,  B01D11/0496 ,  B01F13/0059 ,  B01F13/0093 ,  B01F2005/0031 ,  B01F2005/0034 ,  B01F2215/0431 ,  B01J19/0093 ,  B01J2219/00783 ,  B01J2219/00905 ,  B01J2219/00907 ,  B01L3/502753 ,  B01L2200/0636 ,  B01L2300/0681 ,  B01L2400/0472 ,  B01L2400/086 ,  G01N30/00 ,  G01N30/42 ,  G01N30/6086 ,  G01N30/6095 ,  G01N2001/4061 ,  G01N2030/009 ,  G01N2030/387 ,  G01N2030/407 ,  G01N2030/522

Abstract: Devices for performing liquid extraction of one or more constituents from one fluid to another fluid are provided. In operation, the fluids are separated by channel structures that stabilize the interfacial boundary between the fluids allowing, for example, countercurrent flow and exchange or other flow conditions incompatible with unassisted maintenance of laminar flow. Also provided are channel structures which aid in mixing the fluids. Thin membranes may be formed using liquid extraction devices according to the invention. A process for manufacturing such devices using DRIE is described.

Abstract translation: 提供了用于将一种或多种组分从一种流体液体萃取到另一种流体的装置。 在操作中，流体通过稳定流体之间的界面边界的通道结构分开，允许例如与逆流维持层流不相容的逆流和交换或其它流动条件。 还提供了有助于混合流体的通道结构。 可以使用根据本发明的液体提取装置形成薄膜。 描述了使用DRIE制造这种装置的方法。