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    STABILIZATION OF WHOLE BLOOD SAMPLES
    3.
    发明申请
    STABILIZATION OF WHOLE BLOOD SAMPLES 审中-公开
    全血样品稳定

    公开(公告)号：WO2016022433A1

    公开(公告)日：2016-02-11

    申请号：PCT/US2015/043269

    申请日：2015-07-31

    Applicant: THE GENERAL HOSPITAL CORPORATION

    Inventor： SANDLIN, Rebecca ,  WONG, Keith ,  TESSIER, Shannon ,  TONER, Mehmet

    IPC: G01N33/50

    CPC classification number: A01N1/0231

    Abstract: Methods for stabilizing blood samples, e.g., clinical blood samples, for storage or transportation before use. Exemplary applications include but are not limited to enrichment of leukocyte subtypes such as T-cells or neutrophils for cytokine and immuno-assays; isolation of progenitor cells from cord blood or peripheral blood for transplantation; isolation of fetal cells from the maternal blood for diagnosis; and sorting of circulating tumor cells for cancer detection and therapy.

    Abstract translation: 用于在使用前稳定血液样品例如临床血液样品用于储存或运输的方法。 示例性应用包括但不限于用于细胞因子和免疫测定的白细胞亚型如T细胞或嗜中性粒细胞的富集; 从脐带血或外周血分离祖细胞进行移植; 从母体血液中分离胎儿细胞进行诊断; 和循环肿瘤细胞的分选用于癌症检测和治疗。

    SYSTEMS AND METHODS FOR CELL PRESERVATION
    9.
    发明申请
    SYSTEMS AND METHODS FOR CELL PRESERVATION 审中-公开

    公开(公告)号：WO2004011616A3

    公开(公告)日：2004-02-05

    申请号：PCT/US2003/023553

    申请日：2003-07-28

    Applicant: THE GENERAL HOSPITAL CORPORATION ,  TONER, Mehmet ,  ACKER, Jason ,  CHEN, Tani ,  BHOWMICK, Sankha ,  BAUST, John ,  FOWLER, Alex

    Inventor： TONER, Mehmet ,  ACKER, Jason ,  CHEN, Tani ,  BHOWMICK, Sankha ,  BAUST, John ,  FOWLER, Alex

    IPC: A01N1/02

    Abstract: The present invention generally relates to devices and methods for the preservation of cells using drying, freezing, and other related techniques. In one set of embodiments, the invention allows for the preservation of cells in a dried state. In another set of embodiments, the invention allows for the preservation of cells within a glass or other non-viscous, non-frozen media. In some embodiments, the invention allows for the preservation of cells at temperatures below the freezing point of water, and in some cases at cryogenic temperatures, without inducing ice formation. The cells, in certain embodiments, may be preserved in the presence of intracellular and/or extracellular carbohydrates (which may be the same or different), for example, trehalose and sucrose. Carbohydrates may be transported intracellularly by any suitable technique, for example, using microinjection, or through non-microinjected methods such as through pore-forming proteins, electroporation, heat shock, etc. In certain instances, the glass transition temperature of the cells may be raised, e.g., by transporting a carbohydrate intracellularly. In some cases, the cells may be dried and/or stored, for example, in a substantially moisture-saturated environment or a desiccating environment. The cells may also be stored in a vacuum or a partial vacuum. The cells may be protected from oxygen, moisture, and/or light during storage. In certain cases, an inhibitor, such as a cell death inhibitor, a protease inhibitor, an apoptosis inhibitor, and/or an oxidative stress inhibitor may be used during preservation of the cells. The cells may be stored for any length of time, then recovered to a viable state, e.g., through rehydration, for further use.

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