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    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS
    1.
    发明申请
    PORCINE TORQUE TENO VIRUS VACCINES AND DIAGNOSIS 审中-公开
    猪瘟TENO病毒疫苗及其诊断

    公开(公告)号：WO2011031438A3

    公开(公告)日：2011-06-30

    申请号：PCT/US2010046330

    申请日：2010-08-23

    Applicant: VIRGINIA TECH INTELL PROP ,  MENG XIANG-JIN ,  HUANG YAOWEI

    Inventor： MENG XIANG-JIN ,  HUANG YAOWEI

    IPC: C12N15/34 ,  A01K67/027 ,  A61K39/12 ,  C12N15/63 ,  C12Q1/68 ,  G01N33/569

    CPC classification number: G01N33/56983 ,  A61K39/12 ,  A61K2039/552 ,  C07K14/005 ,  C07K16/081 ,  C12N7/00 ,  C12N2750/00021 ,  C12N2750/00022 ,  C12N2750/00034 ,  C12N2750/14034 ,  C12Q1/701 ,  G01N2333/01 ,  G01N2333/085 ,  G01N2469/20

    Abstract: The present invention provides four purified preparation containing a polynucleic acid molecule encoding porcine Torque teno virus (PTTV) genotypes or subtypes PTTV1a-VA, PTTV1b-VA, PTTV2b-VA, and PTTV2c-VA. The present invention also provides infectious DNA clones, biologically functional plasmid or viral vector containing the infectious nucleic acid genome molecule of the same. The present invention further provides live, attenuated, vector-expressed and purified recombinant capsid subunit or killed viral vaccines for protection against PTTV infection. The present invention additionally provides subunit vaccines comprising PTTV specific gene products, especially ORF1 capsid gene product for protection against PTTV infection. Further, the present invention provides methods for diagnosing PTTV infection via polymerase chain reaction (PCR) using specific primer for PTTV1, PTTV2, and individual PTTV1 genotypes. Finally, the present invention provides methods for diagnosing PTTV infection via immunological methods, e.g., enzyme-linked immunoabsorbent assay (ELISA) and Western blot using PTTV specific antigens for detecting serum PTTV specific antibodies.

    Abstract translation: 本发明提供了四种含有编码猪扭矩型诺沃病毒（PTTV）基因型或亚型PTTV1a-VA，PTTV1b-VA，PTTV2b-VA和PTTV2c-VA的多核酸分子的纯化制剂。 本发明还提供了感染性DNA克隆，生物功能性质粒或含有其的感染性核酸基因组分子的病毒载体。 本发明进一步提供活的，减毒的，载体表达和纯化的重组衣壳亚单位或杀死的病毒疫苗以防止PTTV感染。 本发明另外提供了包含PTTV特异性基因产物的亚单位疫苗，特别是用于防止PTTV感染的ORF1衣壳基因产物。 此外，本发明提供了使用用于PTTV1，PTTV2和个体PTTV1基因型的特异性引物通过聚合酶链式反应（PCR）诊断PTTV感染的方法。 最后，本发明提供了通过免疫学方法诊断PTTV感染的方法，例如酶联免疫吸附测定（ELISA）和使用PTTV特异性抗原的Western印迹来检测血清PTTV特异性抗体。

    ROLLING CIRCLE REPLICON EXPRESSION VECTORS
    2.
    发明申请
    ROLLING CIRCLE REPLICON EXPRESSION VECTORS 审中-公开
    滚动循环回复表达式向量

    公开(公告)号：WO0161024A3

    公开(公告)日：2002-06-27

    申请号：PCT/US0105394

    申请日：2001-02-15

    Applicant: LARGE SCALE BIOLOGY CORP ,  PALMER KENNETH E ,  POGUE GREGORY P

    Inventor： PALMER KENNETH E ,  POGUE GREGORY P

    IPC: C07K14/01 ,  C12N15/34 ,  C12N15/79 ,  C12N15/86

    CPC classification number: C07K14/005 ,  A01K2217/05 ,  C12N15/79 ,  C12N15/86 ,  C12N2750/00022 ,  C12N2750/12043 ,  C12N2800/108 ,  C12N2830/42

    Abstract: A rolling circle DNA replicon which replicates in a host eukaryotic cell is disclosed which has a truncated replication cycle. The rolling circle DNA replicon comprises the following elements present on the same DNA molecule. It contains a Rep gene open reading frame from a virus belonging to the viral taxonomic families Geminiviridae, Circoviridae or genus Nanovirus . The Rep gene open reading frame is placed under transcriptional control of a promoter, which is placed 5' of the gene. Any sequences that are required to be present in cis on the rolling circle DNA replicon in order that the Rep protein might promote replication of the rolling circle DNA replicon are included. An expression cassette for expression of an ancillary protein that is capable of creating a cellular environment permissive for replication of the rolling circle DNA replicon in the host cell of interest is also included. At least one expression cassette with an RNA polymerase II promoter, a multiple cloning site, and transcription termination and polyadenylation signals suitable for transcription of RNA molecules not normally intrinsic to a geminiviral, circoviral or nanoviral genome is also included.

    Abstract translation: 公开了在宿主真核细胞中复制的旋环DNA复制子，其具有截短的复制周期。 滚环DNA复制子包含存在于相同DNA分子上的以下元素。 它包含来自属于病毒分类学家Geminiviridae，Circoviridae或属Nanovirus的病毒的Rep基因开放阅读框。 将Rep基因开放阅读框置于启动子的转录控制下，其位于基因的5'。 包括在循环DNA复制子上需要存在于顺式中的任何序列，以使Rep蛋白可能促进滚环DNA复制子的复制。 还包括用于表达能够产生在感兴趣的宿主细胞中允许循环DNA复制子复制的细胞环境的辅助蛋白质的表达盒。 还包括至少一个具有RNA聚合酶II启动子，多克隆位点和适合于双因素病毒，环状病毒或纳米病毒基因组通常不固有的RNA分子转录的转录终止和多聚腺苷酸化信号的表达盒。

    HEPATITIS VIRUS SENTINEL VIRUS I (SVI)
    5.
    发明申请
    HEPATITIS VIRUS SENTINEL VIRUS I (SVI) 审中-公开
    HEPATITIS VIRUS SENTINEL VIRUS I（SVI）

    公开(公告)号：WO01042299A2

    公开(公告)日：2001-06-14

    申请号：PCT/IB2000/002011

    申请日：2000-12-08

    IPC: G01N33/53 ,  A61K39/00 ,  A61K39/29 ,  A61K39/39 ,  A61P31/12 ,  C07K14/005 ,  C07K14/01 ,  C07K16/08 ,  C12N7/00 ,  C12N15/09 ,  C12P21/08 ,  C12Q1/68 ,  G01N33/566 ,  G01N33/576

    CPC classification number: C07K14/005 ,  A61K39/00 ,  C12N7/00 ,  C12N2710/00021 ,  C12N2710/00022 ,  C12N2750/00022

    Abstract: The invention relates to a new group of viruses, designated SVI. Isolated SVI viruses, polynucleotides and proteins from SVI viruses, and antibodies which bind SVI virus and SVI viral proteins are provided. The polynucleotides, proteins, and antibodies of the invention may be used to detect SVI virus or infection by SVI virus in a susceptible individual. Additionally, polynucleotides of the invention may be inserted into recombinant expression vectors recombinant production of viral proteins.

    Abstract translation: 本发明涉及一组新的病毒，称为SVI。 提供了分离的SVI病毒，来自SVI病毒的多核苷酸和蛋白质，以及结合SVI病毒和SVI病毒蛋白质的抗体。 本发明的多核苷酸，蛋白质和抗体可用于检测易感个体中的SVI病毒或SVI病毒感染。 另外，本发明的多核苷酸可以插入重组表达载体重组生产病毒蛋白质。

    TT-VIRUS-SEQUENZEN IN MENSCHLICHEN TUMORGEWEBEN, MITTEL ZU DEREN NACHWEIS SOWIE TUMORTHERAPIE
    6.
    发明申请
    TT-VIRUS-SEQUENZEN IN MENSCHLICHEN TUMORGEWEBEN, MITTEL ZU DEREN NACHWEIS SOWIE TUMORTHERAPIE 审中-公开
    人肿瘤TT病毒序列编织检测和肿瘤的治疗将它们

    公开(公告)号：WO2003023027A2

    公开(公告)日：2003-03-20

    申请号：PCT/DE2002/003371

    申请日：2002-09-10

    Applicant: DEUTSCHES KREBSFORSCHUNGSZENTRUM ,  ZUR HAUSEN, Harald ,  DE VILLIERS-ZUR HAUSEN, Ethel-Michele

    Inventor： ZUR HAUSEN, Harald ,  DE VILLIERS-ZUR HAUSEN, Ethel-Michele

    IPC: C12N9/00

    CPC classification number: C07K14/005 ,  C12N2750/00022

    Abstract: Beschrieben werden DNA-Sequenzen, die das Genom eines Tumorassoziierten TT-Virus umfassen, oder Fragmente davon. Die vorliegende Erfindung betrifft ferner von diesen DNA-Sequenzen kodierte Proteine, gegen diese Proteine gerichtete Antikörper sowie gegen mRNAs gerichtete Ribozyme und Antisense-RNAs und schliesslich deren Verwendung in Diagnose und Therapie. Die vorliegende Erfindung betrifft auch zur Diagnose geeignete Reagenzien enthaltende Kits, z.B. Antikörper enthaltende Kits.

    Abstract translation: 描述的是其包含肿瘤相关TT病毒的基因组的DNA序列，或其片段。 本发明还涉及一种编码针对这些蛋白质和针对mRNA的核酶和反义RNA的抗体蛋白这些DNA序列，最后它们在诊断和治疗中的用途。 本发明还涉及含有该诊断试剂盒合适的试剂，例如 包含抗体。

    ROLLING CIRCLE REPLICON EXPRESSION VECTORS
    8.
    发明申请
    ROLLING CIRCLE REPLICON EXPRESSION VECTORS 审中-公开
    滚动循环回复表达式向量

    公开(公告)号：WO01061024A2

    公开(公告)日：2001-08-23

    申请号：PCT/US2001/005394

    申请日：2001-02-15

    IPC: C07K14/01 ,  C12N15/34 ,  C12N15/79 ,  C12N15/86

    CPC classification number: C07K14/005 ,  A01K2217/05 ,  C12N15/79 ,  C12N15/86 ,  C12N2750/00022 ,  C12N2750/12043 ,  C12N2800/108 ,  C12N2830/42

    Abstract: A rolling circle DNA replicon which replicates in a host eukaryotic cell is disclosed which has a truncated replication cycle. The rolling circle DNA replicon comprises the following elements present on the same DNA molecule. It contains a Rep gene open reading frame from a virus belonging to the viral taxonomic families Geminiviridae, Circoviridae or genus Nanovirus . The Rep gene open reading frame is placed under transcriptional control of a promoter, which is placed 5' of the gene. Any sequences that are required to be present in cis on the rolling circle DNA replicon in order that the Rep protein might promote replication of the rolling circle DNA replicon are included. An expression cassette for expression of an ancillary protein that is capable of creating a cellular environment permissive for replication of the rolling circle DNA replicon in the host cell of interest is also included. At least one expression cassette with an RNA polymerase II promoter, a multiple cloning site, and transcription termination and polyadenylation signals suitable for transcription of RNA molecules not normally intrinsic to a geminiviral, circoviral or nanoviral genome is also included.

    Abstract translation: 公开了在宿主真核细胞中复制的旋环DNA复制子，其具有截短的复制周期。 滚环DNA复制子包含存在于相同DNA分子上的以下元素。 它包含来自属于病毒分类学家双子座科，圆环病毒科或纳米病毒属的病毒的Rep基因开放阅读框架。 将Rep基因开放阅读框置于启动子的转录控制下，其位于基因的5'。 包括在循环DNA复制子上需要存在于顺式中的任何序列，以使Rep蛋白可能促进滚环DNA复制子的复制。 还包括用于表达能够产生在感兴趣的宿主细胞中允许循环DNA复制子复制的细胞环境的辅助蛋白质的表达盒。 还包括至少一个具有RNA聚合酶II启动子，多克隆位点以及适合于双因素病毒，环状病毒或纳米病毒基因组通常不固有的RNA分子转录的转录终止和多聚腺苷酸化信号的表达盒。

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