GENE ENCODING A MUTANT PROTEIN PROVIDING A DECORATIVE FLOWERING PHENOTYPE IN PLANTS
    3.
    发明申请
    GENE ENCODING A MUTANT PROTEIN PROVIDING A DECORATIVE FLOWERING PHENOTYPE IN PLANTS 审中-公开
    编码在植物中提供装饰性花粉的突变蛋白的基因

    公开(公告)号:WO2015086165A1

    公开(公告)日:2015-06-18

    申请号:PCT/EP2014/063019

    申请日:2014-06-20

    Applicant: FIDES BV

    Abstract: The present invention relates to proteins capable of providing a decorative flowering phenotype in plants and especially in plants belonging to the Kalanchoe genus. The present invention also relates to nucleic acid sequences, or cDNA sequences, and genes encoding the present proteins. The present invention further relates to use of the present proteins, nucleic acid sequences and genes for selecting decorative flowering Kalanchoe plants and decorative flowering Kalanchoe plants comprising the present proteins, mRNA forms of the present cDNAs or the present genes. Specifically, the present invention relates to proteins comprising an amino acid substitution of the amino acid histidine at position 136 and/or the amino acid alanine at position 338 of the methyltransf erase protein of a Kalanchoe plant, wherein the amino acid substitution provides a decorative flowering phenotype in the present Kalanchoe plants.

    Abstract translation: 本发明涉及能够在植物中,特别是属于Kalanchoe属的植物中提供装饰开花表型的蛋白质。 本发明还涉及编码本蛋白质的核酸序列或cDNA序列和基因。 本发明还涉及本发明的蛋白质,核酸序列和基因用于选择装饰开花的Kalanchoe植物和装饰开花的Kalanchoe植物,其包含本发明的蛋白质,本发明的cDNA或本基因的mRNA形式。 具体地说,本发明涉及包含卡兰切荷植物的甲基转移消除蛋白的第136位的氨基酸组氨酸和/或第338位的氨基酸丙氨酸的氨基酸取代的蛋白质,其中氨基酸取代提供装饰开花 在当前的Kalanchoe植物中的表型。

    C1ORF59 FOR TARGET GENES OF CANCER THERAPY AND DIAGNOSIS
    4.
    发明申请
    C1ORF59 FOR TARGET GENES OF CANCER THERAPY AND DIAGNOSIS 审中-公开
    C1ORF59针对目标癌症治疗和诊断基因

    公开(公告)号:WO2011161960A1

    公开(公告)日:2011-12-29

    申请号:PCT/JP2011/003564

    申请日:2011-06-22

    Abstract: Objective methods for diagnosing a predisposition to developing cancer are described herein. In one embodiment, the diagnostic method involves determining an expression level of the C1orf59 gene, the PIWIL4 gene, or piRNA and correlating the determined level with a healthy, diseased or at-risk state. The present invention further provides methods of assessing or determining the prognosis of a patient with esophageal cancer. In one embodiment, the diagnostic method involves determining an expression level of the C1orf59 gene. The present invention further provides methods of screening for therapeutic substances useful in the treatment of a C1orf59-, and/or PIWIL4-associated disease, such as a cancer. The present invention further provides methods of treating or preventing a cancer expressing the C1orf59 gene and/or the PIWIL4 gene. The present invention also features products, including double-stranded molecules and vectors encoding thereof as well as to compositions including them.

    Abstract translation: 本文描述了用于诊断发展中的癌症倾向的客观方法。 在一个实施方案中,诊断方法包括确定C1orf59基因,PIWIL4基因或piRNA的表达水平,并将确定的水平与健康的,患病的或处于危险的状态相关联。 本发明还提供了评估或确定患有食管癌患者的预后的方法。 在一个实施方案中,诊断方法涉及确定C1orf59基因的表达水平。 本发明还提供筛选用于治疗C1orf59-和/或PIWIL4相关疾病如癌症的治疗物质的方法。 本发明还提供了治疗或预防表达C1orf59基因和/或PIWIL4基因的癌症的方法。 本发明还包括产物,包括双链分子和编码它们的载体以及包括它们的组合物。

    NOVEL PROTEIN AND GENE RELATED TO FLAVONOID O-METHYLTRANSFERASE (FOMT) AND THEIR USES THEREFORE
    7.
    发明申请
    NOVEL PROTEIN AND GENE RELATED TO FLAVONOID O-METHYLTRANSFERASE (FOMT) AND THEIR USES THEREFORE 审中-公开
    与FLAVONOID O-甲基转移酶(FOMT)相关的新蛋白和基因及其用途

    公开(公告)号:WO2014176732A1

    公开(公告)日:2014-11-06

    申请号:PCT/CN2013/074973

    申请日:2013-04-28

    CPC classification number: C12N15/825 C12N9/1007 C12N15/8205 C12Y201/01

    Abstract: The present invention provides novel protein and gene related to flavonoid O-methyltransferase (FOMT) and their uses therefore. The said protein having an amino acid sequence shown in SEQ ID NO: 3, or an amino acid sequence having deletion, substitution or insertion of one or plural amino acids in said amino acid sequence. The said gene comprising the nucleotide sequence shown in SEQ ID NO: 1, or a gene which hybridizes with said gene under stringent conditions and encodes a protein, which has anthocyanin 3'-O-methyltransferase or 3',5'-O-methyltrasnferase activity. The present invention also provides a method for obtaining the transgenic plant used the above-mentioned gene.

    Abstract translation: 本发明提供与类黄酮O-甲基转移酶(FOMT)相关的新型蛋白质和基因及其用途。 所述蛋白质具有SEQ ID NO:3所示的氨基酸序列,或在所述氨基酸序列中具有一个或多个氨基酸的缺失,取代或插入的氨基酸序列。 所述基因包含SEQ ID NO:1所示的核苷酸序列,或在严格条件下与所述基因杂交并编码具有花青素3'-O-甲基转移酶或3',5'-O-甲基酪氨酸激酶的蛋白质的基因 活动。 本发明还提供了获得使用上述基因的转基因植物的方法。

    VERFAHREN ZUR BIOTECHNOLOGISCHEN HERSTELLUNG VON FLAVONOIDEN
    9.
    发明申请
    VERFAHREN ZUR BIOTECHNOLOGISCHEN HERSTELLUNG VON FLAVONOIDEN 审中-公开
    方法为生物技术制造类黄酮

    公开(公告)号:WO2016050656A1

    公开(公告)日:2016-04-07

    申请号:PCT/EP2015/072189

    申请日:2015-09-27

    Applicant: SYMRISE AG

    Abstract: Vorgeschlagen wird ein Verfahren zur Herstellung von Flavonoiden, umfassend die Schritte: (a) Bereitstellen eines transgenen Mikroorganismus, enthaltend (i) einen ersten Nukleinsäure-Abschnitt (A), umfassend oder bestehend aus einem für eine CYP450-Oxidase kodierenden Gen, (ii) einen zweiten Nukleinsäure-Abschnitt (B), umfassend oder bestehend aus einem für eine pflanzliche O-Methyltransferase kodierenden Gen, sowie (b) Hinzufügen eines oder mehrerer Flavanone zu dem transgenen Mikroorganismus, (c) die Umsetzung der Substrat Flavanone durch des transgenen Mikroorganismus zu den entsprechenden Flavonoiden, sowie gegebenenfalls (d) Isolierung und Aufreinigung der Endprodukte.

    Abstract translation: 提出一种方法,用于生产类黄酮的方法,包括以下步骤:(a)提供转基因微生物,其包含(i)第一核酸部分(A),其包含或由一个基因的编码CYP450氧化酶基因,(ⅱ) 第二核酸部分(b),其包含或由一个基因的编码植物-O-甲基转移酶基因,和(b)加入一种或多种的黄烷酮向转基因微生物,(c)该基材黄烷酮由转基因微生物反应 最终产品的相应的黄酮类化合物,和任选的(d)中分离和纯化。

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