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    MILD HYDROLYSIS OF PROTEINS FROM RICE BRAN
    4.
    发明申请
    MILD HYDROLYSIS OF PROTEINS FROM RICE BRAN 审中-公开
    米兰蛋白质水解研究

    公开(公告)号：WO2014064024A1

    公开(公告)日：2014-05-01

    申请号：PCT/EP2013/071914

    申请日：2013-10-21

    Applicant: DSM IP ASSETS B.V.

    Inventor： JANSE, Arthur, Maurits, Christiaan ,  VEERMAN, Cecile ,  SMOLDERS, Gerardus, Johannes, Franciscus

    IPC: A23J3/34 ,  A23J1/12 ,  A23L1/305

    CPC classification number: A23J3/346 ,  A23J1/125 ,  A23J3/14 ,  A23L33/18 ,  A23V2002/00 ,  C12P21/06 ,  C12Y304/11 ,  C12Y304/21 ,  C12Y304/22 ,  C12Y304/23 ,  C12Y304/24 ,  C12Y304/24028 ,  C12Y304/25 ,  A23V2250/548 ,  A23V2250/55

    Abstract: The present invention provides a rice bran hydrolysate which comprises of more than 90%, preferably of more than 95%, of (poly) peptides with a molecular weight (MW) of more than 500 Da and which has a DH (Degree of Hydrolysis) of at least 10%, preferably between 10 and 16%. According to another aspect of the invention a process to produce a rice bran hydrolysate is provided which comprises: adding an enzyme or enzyme composition to a suspension of rice bran which has a concentration of rice bran of between 12 and 30 wt%; performing an enzyme incubation at a pH between 6 and 8; performing the enzyme incubation to an extend of hydrolysis to a DH (Degree of Hydrolysis) of at least 10%, preferably between 10 and 16%; performing the enzyme incubation at a temperature of between 45 and 65 °C; and separating the liquid from the solid fraction; whereby the enzyme or enzyme composition comprises an endoprotease.

    Abstract translation: 本发明提供了一种米糠水解产物，其包含分子量（MW）大于500Da并且具有DH（水解度）的超过90％，优选大于95％的（多）肽， 至少10％，优选10至16％。 根据本发明的另一方面，提供了一种生产米糠水解产物的方法，其包括：向米糠浓度为12-30重量％的米糠悬浮液中加入酶或酶组合物; 在6至8之间的pH下进行酶温育; 将酶水解延伸至DH（水解度）至少10％，优选10至16％之间进行酶温育; 在45至65℃的温度下进行酶温育; 并将液体与固体部分分离; 由此酶或酶组合物包含内切蛋白酶。

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