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    METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELL, COMPOSITION USED IN METHOD, AND USES THEREOF
    3.
    发明公开
    METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELL, COMPOSITION USED IN METHOD, AND USES THEREOF 审中-公开
    VERFAHREN ZUR HERSTELLUNG INDUZIERTER PLURIPOTENTER STAMMZELLEN，ZUSAMMENSETZUNGHIERFÜRUND VERWENDUNGEN DAVON

    公开(公告)号：EP3070163A4

    公开(公告)日：2017-06-28

    申请号：EP14862407

    申请日：2014-11-12

    申请人： GUANGZHOU INST BIOMED & HEALTH

    发明人： PEI DUANQING ,  LIU JING ,  CHEN JIEKAI ,  PENG MEIXIU

    IPC分类号： C12N5/074 ,  C07K14/435

    CPC分类号： C12N5/0696 ,  C12N2501/60 ,  C12N2501/602 ,  C12N2501/603 ,  C12N2501/604 ,  C12N2501/605 ,  C12N2501/606 ,  C12N2501/608 ,  C12N2506/02

    摘要： Provided are a method for preparing an induced pluripotent stem cell and a composition used in the method. The method comprises: introducing a composition for promoting the formation of an induced pluripotent stem cell into a somatic cell, the composition comprising: (i) a c-Jun antagonist and one group of factors from among the following seven such groups: (1) Sox2, Klf4 and c-Myc, (2) Klf4 and c-Myc, (3) Oct3/4, Klf4 and c-Myc, (4) Sox2, Nanog and Lin28, (5) Oct3/4, Nanog and Lin28, (6) Oct3/4, Klf and Sox2, and (7) Klf4 and Sox2; or (ii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of Glis1, Sall4 or Lrh1; or (iii) the c-Jun antagonist, Jhdm1b and Id1, and at least one of: Oct4, Klf4, Sox2, Lin28, Esrrb, Lef1, Utf1 or miRNA C. The present method allows for successful preparation of induced pluripotent stem cells with no generation of abnormal chromosomes.

    摘要翻译： 提供了用于制备诱导性多能干细胞的方法和用于该方法中的组合物。 所述方法包括：将用于促进诱导性多能干细胞形成的组合物引入体细胞中，所述组合物包含：（i）c-Jun拮抗剂和来自以下七个这样的组中的一组因子：（1） Sox2，Klf4和c-Myc，（2）Klf4和c-Myc，（3）Oct3 / 4，Klf4和c-Myc，（4）Sox2，Nanog和Lin28，（5）Oct3 / 4，Nanog和Lin28， （6）Oct3 / 4，Klf和Sox2，和（7）Klf4和Sox2; 或（ii）c-Jun拮抗剂Jhdm1b和Id1以及Glis1，Sall4或Lrh1中的至少一种; 或（iii）c-Jun拮抗剂，Jhdm1b和Id1以及Oct4，Klf4，Sox2，Lin28，Esrrb，Lef1，Utf1或miRNAC中的至少一种。本发明方法允许成功制备诱导性多能干细胞 没有一代异常的染色体。

    METHOD FOR INTRODUCING EXOGENOUS MITOCHONDRIA INTO MAMMALIAN CELLS
    6.
    发明公开
    METHOD FOR INTRODUCING EXOGENOUS MITOCHONDRIA INTO MAMMALIAN CELLS 审中-公开
    VÄRFAHRENZUREINFÜHRUNGVON EXOGEN MITOCHONDRIEN在SÄUGETIERZELLEN

    公开(公告)号：EP3067416A4

    公开(公告)日：2017-10-04

    申请号：EP14860811

    申请日：2014-08-25

    申请人： GUANGZHOU INST BIOMED & HEALTH

    发明人： LIU XINGGUO ,  PEI DUANQING ,  LIU JINGLEI ,  LIU XUEBIN ,  YAO DEYANG

    IPC分类号： C12N5/10 ,  C12N5/071 ,  C12N5/0786 ,  C12N15/85 ,  C12N15/87

    CPC分类号： C12N5/0645 ,  C12N15/87 ,  C12N2510/00

    摘要： The present disclosure provides a method for producing a cell with exogenous mitochondria by obtaining synthetic mitochondria via introduction of exogenous mitochondrial DNA into mitochondria or empty mitochondrial shells, and incorporating the same into mammalian cells via endocytosis. As such, effective functionality of exogenous mitochondria in cells is realized. The synthetic mitochondrial DNA genes introduced according to the present disclosure can be stably expressed and effectively passaged. The method for introducing exogenous mitochondrial DNA into mammalian cells as disclosed herein may be used as a whole new mitochondrial molecular cloning means to perform site-directed mutagenesis, gene insertion, gene knockout, gene rearrangement, and the like in mitochondria. Therefore, any molecular cloning modification can be performed on a mammalian mitochondrial DNA, which is of great importance to therapeutic schemes of diseases derived from mitochondrial DNA mutations.

    摘要翻译： 本公开提供了一种通过将外源线粒体DNA导入线粒体或空线粒体壳获得合成线粒体并通过胞吞作用将其掺入哺乳动物细胞中来产生具有外源线粒体的细胞的方法。 如此，实现了细胞中外源线粒体的有效功能。 根据本公开引入的合成线粒体DNA基因可以稳定表达并有效传代。 如本文所公开的用于将外源线粒体DNA导入哺乳动物细胞的方法可以用作整个新的线粒体分子克隆方法以在线粒体中进行定点诱变，基因插入，基因敲除，基因重排等。 因此，可以对哺乳动物线粒体DNA进行任何分子克隆修饰，这对线粒体DNA突变导致的疾病的治疗方案非常重要。