公开(公告)号：EP2410337A4

公开(公告)日：2012-12-12

申请号：EP10753559

申请日：2010-03-17

申请人： WAKO PURE CHEM IND LTD

发明人： YONEDA AKITO

IPC分类号： G01N33/579 ,  C07K14/435 ,  C12N1/15 ,  C12N1/21 ,  C12N5/00 ,  C12N15/09 ,  C12P21/02 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/579 ,  C07K14/43509 ,  C12N9/6408 ,  G01N33/5308 ,  G01N33/56961 ,  G01N2333/38 ,  G01N2333/40 ,  G01N2333/43504 ,  G01N2400/24 ,  G01N2469/10 ,  Y10S435/935

摘要： Disclosed are a method for measuring ²G comprising the steps of bringing a sample into contact with a ²G-binding protein 1 and a ²G-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID N0:20, and having the ²-glucan binding activity, to form a complex of the ²G-binding protein 1, ²G in the sample and the ²G-binding protein 2, measuring quantity of the complex, and determining ²G concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel ²G-binding protein; a nucleic acid molecule encoding the ²G-binding protein; and a method for producing the aforementioned ²G-binding protein.

摘要翻译： 公开了一种测量G的方法，包括使样品与G结合蛋白1和G结合蛋白2接触的步骤，每个包含与SEQ ID NO：1所示的氨基酸序列相同或基本相同的氨基酸序列 SEQ ID NO：4，SEQ ID NO：6，SEQ ID NO：8，SEQ ID NO：10，SEQ ID NO：14，SEQ ID NO：16，SEQ ID NO：18或SEQ ID NO： 20，并具有β-葡聚糖结合活性，形成样品中的G结合蛋白1，G和G结合蛋白2的复合物，测定复合物的量，并基于 复数的数量; 用于所述方法的试剂和试剂盒; 一种新型的G结合蛋白; 编码该结合蛋白的核酸分子; 以及制备上述的G结合蛋白的方法。

公开(公告)号：EP0915988B1

公开(公告)日：2003-01-29

申请号：EP98929264.4

申请日：1998-04-22

申请人： DSM N.V.

发明人： NIEBOER, Maarten ,  DE VROOM, Erik ,  LUGTENBURG, Johannis ,  SCHIPPER, Dirk ,  VOLLEBREGT, Adrianus, Wilhelmus, Hermanus ,  BOVENBERG, Roelof, Ary, Lans

IPC分类号： C12P35/00 ,  C12P37/04 ,  C12N15/52 ,  C12N15/54

CPC分类号： C12N9/0071 ,  C12N9/1029 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

摘要： The present invention discloses a process for the production of N-deacylated cephalosporin compounds via the fermentative production of their 7-acylated counterparts.

公开(公告)号：EP0235951A1

公开(公告)日：1987-09-09

申请号：EP87300916.1

申请日：1987-02-02

申请人： Antibioticos, S.A.

发明人： Sanchez, Florentina Sanchez ,  Susan, Victor Rubio ,  Soto, Miguel Angel Penalva ,  Ortega, Agustin Perez Aranda

IPC分类号： C12N15/80

CPC分类号： C12N9/0028 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/80 ,  Y10S435/935

摘要： Auxotrophic mutants of P. chrysogenum are of use as receptors for exogenous DNA, facilitating genetic engineering of this industrially useful fungus.

摘要翻译： 产黄青素的营养突变体可用作外源DNA的受体，有助于该工业上有用的真菌的遗传工程。

公开(公告)号：EP0540210B1

公开(公告)日：1998-12-02

申请号：EP92309351.2

申请日：1992-10-14

申请人： GIST-BROCADES B.V.

发明人： Conder, Michael J. ,  Rambosek, John A. ,  Stepan, Anthony Michael ,  Mcada, Phyllis C. ,  Crawford, Lorilee ,  Reeves, Christopher D.

IPC分类号： C12N15/52 ,  C12N1/15 ,  C12P35/02

CPC分类号： C12N9/0071 ,  C12N9/00 ,  C12N9/1029 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

公开(公告)号：EP0843013A3

公开(公告)日：1998-06-17

申请号：EP97203374.0

申请日：1992-09-11

申请人： GIST-BROCADES B.V.

发明人： Crawford, Lorilee ,  Conder, Michael J. ,  McAda, Phyllis C. ,  Rambosek, John A.

IPC分类号： C12N15/52 ,  C12P35/00 ,  C12N9/00 ,  C12N1/21

CPC分类号： C12N9/0071 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

摘要： An important intermediate for preparing cephalopsporin antibotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus , with which the P.chrysogenum has been transformed, converts the adipoyl-6-APA by ring expansion to the novel compound adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.

摘要翻译： 制备头孢菌素类抗生素7-氨基脱乙酰氧基头孢菌酸（7-ADCA）的重要中间体是通过新型生物过程制备的，其中在己二酸酯原料存在下培养转化的产黄青霉菌菌株以产生己二酰-6-APA（6 - 氨基青霉烷酸）; 并且就地表达扩环酶基因，例如来自链霉菌（Streptomyces clavuligerus）的，已经转化产青霉菌的扩环酶基因通过环扩环将己二酰-6-APA转化为新化合物己二酰-7-ADCA。 然后使用己二酰基酰基转移酶切割己二酰侧链制备最终产物7-ADCA。 相应地，整个合成使用生物过程进行，并且是高效和经济的。

公开(公告)号：EP0240250B1

公开(公告)日：1992-07-01

申请号：EP87302632.2

申请日：1987-03-26

申请人： Antibioticos, S.A.

发明人： Sanchez, Florentina Sanchez ,  Susan, Victor Rubio ,  Carramolino-Fitera, Laura ,  Ortega, Agustin Perez Aranda

IPC分类号： C12N15/52 ,  C12N15/53 ,  C12N15/80

CPC分类号： C12N9/0028 ,  C12N9/1085 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12N15/80 ,  Y10S435/911 ,  Y10S435/933 ,  Y10S435/935

公开(公告)号：EP2410337A1

公开(公告)日：2012-01-25

申请号：EP10753559.3

申请日：2010-03-17

申请人： Wako Pure Chemical Industries, Ltd.

发明人： YONEDA Akito

IPC分类号： G01N33/579 ,  C07K14/435 ,  C12N1/15 ,  C12N1/21 ,  C12N5/00 ,  C12N15/09 ,  C12P21/02 ,  G01N33/53 ,  G01N33/543

CPC分类号： G01N33/579 ,  C07K14/43509 ,  C12N9/6408 ,  G01N33/5308 ,  G01N33/56961 ,  G01N2333/38 ,  G01N2333/40 ,  G01N2333/43504 ,  G01N2400/24 ,  G01N2469/10 ,  Y10S435/935

摘要： Disclosed are a method for measuring βG comprising the steps of bringing a sample into contact with a βG-binding protein 1 and a βG-binding protein 2, each comprising an amino acid sequence which is identical or substantially identical to an amino acid sequence shown in any one of SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:14, SEQ ID NO:16, SEQ ID NO:18, or SEQ ID N0:20, and having the β-glucan binding activity, to form a complex of the βG-binding protein 1, βG in the sample and the βG-binding protein 2, measuring quantity of the complex, and determining βG concentration in the sample based on the quantity of the complex; a reagent and a kit for use in said method; a novel βG-binding protein; a nucleic acid molecule encoding the βG-binding protein; and a method for producing the aforementioned βG-binding protein.

公开(公告)号：EP0882131A1

公开(公告)日：1998-12-09

申请号：EP97905653.0

申请日：1997-02-07

申请人： NOVO NORDISK BIOTECH, INC. ,  NOVO NORDISK A/S

发明人： BERKA, Randy, M. ,  CHRISTGAU, Stephan ,  SHUSTER, Jeff ,  HALKIER, Torben ,  FUGLSANG, Claus, Crone

IPC分类号： C12N15 ,  C07K19 ,  C12N1 ,  C12N9 ,  C12R1

CPC分类号： C12Y302/01084 ,  C12N9/2402 ,  Y10S435/933 ,  Y10S435/935

摘要： The present invention relates to polypeptides having mutanase activity and isolated nucleic acid sequences encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the polypeptides. The present invention further relates to oral cavity compositions and methods for degrading mutan.

公开(公告)号：EP0843013A2

公开(公告)日：1998-05-20

申请号：EP97203374.0

申请日：1992-09-11

申请人： GIST-BROCADES B.V.

发明人： Crawford, Lorilee ,  Conder, Michael J. ,  McAda, Phyllis C. ,  Rambosek, John A.

IPC分类号： C12N15/52 ,  C12P35/00 ,  C12N9/00 ,  C12N1/21

CPC分类号： C12N9/0071 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

摘要： An important intermediate for preparing cephalopsporin antibotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus , with which the P.chrysogenum has been transformed, converts the adipoyl-6-APA by ring expansion to the novel compound adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.

摘要翻译： 通过新型生物工艺制备头孢菌素类抗生素7-氨基乙酰氧基头孢菌素酸（7-ADCA）的重要中间体，其中转化的产黄青霉菌株在己二酸原料存在下培养以产生己二酰基-6-APA（6 氨基青霉烷酸）; 并且例如来自产黄青霉的链霉菌（Streptomyces clavuligerus）的扩环酶基因的原位表达通过环扩增将己二酰基-6-APA转化为新化合物己二酰-7-ADCA。 然后通过使用己二酰酰化酶裂解己二酰侧链制备最终产物7-ADCA。 因此，整个合成使用生物工艺进行，并且是高效和经济的。

公开(公告)号：EP0532341A1

公开(公告)日：1993-03-17

申请号：EP92308286.1

申请日：1992-09-11

申请人： GIST-BROCADES B.V.

发明人： Conder, Michael J. ,  Crawford, Lorilee ,  McAda, Phyllis C. ,  Rambosek, John A.

IPC分类号： C12N15/52 ,  C12N1/15 ,  C12N9/00 ,  C12P35/02

CPC分类号： C12N9/0071 ,  C12P35/00 ,  C12P37/04 ,  Y10S435/935

摘要： An important intermediate for preparing cephalosporin antibiotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus , with which the P. chrysogenum has been transformed, converts the adipoyl-6-APA by ring expansion to adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.

摘要翻译： 通过新型生物工艺制备头孢菌素类抗生素7-氨基乙酰氧基头孢菌素酸（7-ADCA）的重要中间体，其中转化的产黄青霉菌株在己二酸原料存在下培养产生己二酰基-6-APA（6 氨基青霉烷酸）; 并且已经转化了产黄青霉的膨胀酶基因例如来自链霉菌的原位表达通过环扩展将己二酰基-6-APA转化为己二酰-7-ADCA。 然后通过使用己二酰酰化酶裂解己二酰侧链制备最终产物7-ADCA。 因此，整个合成使用生物工艺进行，并且是高效和经济的。