利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

174 条记录 (耗时 0.018 秒)

    MULTIMERIC PROTEINS
    94.
    发明授权
    MULTIMERIC PROTEINS 无效
    多聚蛋白

    公开(公告)号：EP0827544B1

    公开(公告)日：2004-08-18

    申请号：EP96916159.5

    申请日：1996-05-23

    申请人： MorphoSys AG

    发明人： PACK, Peter ,  HOESS, Adolf

    IPC分类号： C12N15/62 ,  C07K19/00 ,  C07K14/82 ,  C07K14/52 ,  C07K14/78 ,  C07K14/47 ,  C07K16/18 ,  C07K16/28 ,  C12N1/21 ,  C12N1/19

    CPC分类号： C07K16/18 ,  A61K38/00 ,  C07K16/28 ,  C07K16/44 ,  C07K19/00 ,  C07K2319/02 ,  C07K2319/70 ,  C07K2319/75 ,  C12N15/62

    摘要： The present invention relates to the construction and use of small multimerization devices, preferably of human origin, which self-assemble fused functional domains to multimeric and multifunctional complexes. Multimerization devices of this invention do not significantly interfere with secretion, expression yields and the independent folding of functional domains, which are attached via flexible but protease-resistant linkers. Modular gene cassettes encoding functional domains, linkers and multimerization domain can easily be combined to a cistron encoding the multimeric protein. Translation in a suitable host results in self-assembly to multimers larger than dimers. In cases, in which one or both functional domains are not expressible in sufficient yields or native fold in the same expression host, multimeric proteins can be produced, in which one or both functional domains or the multimerization device are produced separately by, e.g., in vitro translation, peptide synthesis and/or refolding and subsequently, e.g., chemically coupled to the remaining part of the multimeric protein.

    GENERATION OF SPECIFIC BINDING PARTNERS BINDING TO (POLY)PEPTIDES ENCODED BY GENOMIC DNA FRAGMENTS OR ESTS
    96.
    发明公开
    GENERATION OF SPECIFIC BINDING PARTNERS BINDING TO (POLY)PEPTIDES ENCODED BY GENOMIC DNA FRAGMENTS OR ESTS 有权
    特异性生产基因组DNA片段或ESTS CODED结合伴侣ON（多）肽BINDING

    公开(公告)号：EP1133565A2

    公开(公告)日：2001-09-19

    申请号：EP00947904.9

    申请日：2000-06-30

    申请人： MorphoSys AG

    发明人： FRISCH, Christian ,  KRETZSCHMAR, Titus ,  HÖSS, Adolf ,  VON RÜDEN, Thomas

    IPC分类号： C12N15/62 ,  C07K16/00 ,  C12N1/21 ,  C12N15/70

    CPC分类号： C40B40/02 ,  C07K16/1018 ,  C07K16/2845 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2319/00 ,  C12N15/1037

    摘要： The present invention relates to the generation of specific binding partners binding to (poly)peptides encoded by genomic DNA fragments or ESTs. The (poly)peptides are expressed as part of fusion proteins which are forming inclusion bodies on expression in host cells. The inclusion bodies are used to generate binding partners which bind specifically to said (poly)peptides. The specific binding partners, in particular immunoglobulins or fragments thereof, are useful for analysis and functional characterisation of proteins encoded by nucleic acid sequences comprising the corresponding genomic DNA fragments or ESTs. The invention further relates to nucleic acid molecules, vectors and host cells to be used in the methods of the present invention. The invention further relates to the use of fusion proteins comprising the first N-terminal domain of the geneIII protein of filamentous phage as fusion partner for the expression of a (poly)peptide/protein fused to said fusion partner, and to methods for the expression of (poly)peptide/proteins.