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    ACTIVE SITE INACTIVATORS
    102.
    发明公开
    ACTIVE SITE INACTIVATORS 有权
    活跃中心的失控者

    公开(公告)号：EP1379634A4

    公开(公告)日：2004-09-08

    申请号：EP02728885

    申请日：2002-04-22

    申请人： UNIV GEORGIA RES FOUND

    发明人： BAIG SALMAN

    IPC分类号： G01N33/53 ,  C12N9/50 ,  C12N9/99 ,  G01N24/08 ,  G01N33/573 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/44

    CPC分类号： C12N9/50 ,  C12N9/99 ,  G01N24/08

    摘要： A method for identifying active site inhibitors of scission enzyme that cleaves the backbones or side groups of peptides, and for using the resultant inactivators to inhibit the target scission enzyme. Kinetic assays are employed to identify peptide substrates that tightly bind to the active site of the target protease but that are not easily cleaved. These noncleavable but tightly binding substrates are optionally structurally modified to yield inhibitory compounds with optimized properties. Additionally these substrates exhibit apparent specificity for a transition state or ground state configuration of the protease.

    POSITIVE RESPONSE BIOSENSORS AND OTHER SENSORS
    104.
    发明公开
    POSITIVE RESPONSE BIOSENSORS AND OTHER SENSORS 有权
    与积极响应和其他传感器生物传感器

    公开(公告)号：EP1430142A2

    公开(公告)日：2004-06-23

    申请号：EP02731594.4

    申请日：2002-05-02

    申请人： Agentase, LLC

    发明人： LEJEUNE, Keith, E. ,  ERBELDINGER, Markus

    IPC分类号： C12Q1/34 ,  C12Q1/44 ,  C12Q1/00 ,  C12M1/00 ,  C12M1/34 ,  G01N33/53

    CPC分类号： C12Q1/001 ,  G01N33/5438 ,  G01N33/94 ,  Y10S435/963

    摘要： A sensor for detecting an analyte in an environment includes a first reaction system including a first enzyme and a substrate for the first enzyme. The analyte inhibits the reaction of the substrate catalyzed by the first enzyme. The sensor further includes at least a second reaction system that reacts to produce a first detectable state when the first enzyme is inhibited. In some embodiments, the reaction of the first reaction system can produce a second detectible state, different from the first detectible state. Another sensor for detecting an analyte in an environment includes a first reaction system including a first enzyme or a first substrate for the first enzyme. In this embodiment, the analyte is either a substrate for the first enzyme or the first enzyme if the first reaction system includes the first substrate. Figure (1) illustrates reaction schematics of one embodiment of a sensor of the present invention for detecting nerve agents.

    ACTIVE SITE INACTIVATORS
    106.
    发明公开
    ACTIVE SITE INACTIVATORS 有权
    灭活活性中心

    公开(公告)号：EP1379634A1

    公开(公告)日：2004-01-14

    申请号：EP02728885.1

    申请日：2002-04-22

    申请人： University of Georgia Research Foundation, Inc.

    发明人： BAIG, Salman

    IPC分类号： C12N9/50 ,  C12N9/99 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/42 ,  C12Q1/44

    CPC分类号： C12N9/50 ,  C12N9/99 ,  G01N24/08

    摘要： A method for identifying active site inhibitors of scission enzyme that cleaves the backbones or side groups of peptides, and for using the resultant inactivators to inhibit the target scission enzyme. Kinetic assays are employed to identify peptide substrates that tightly bind to the active site of the target protease but that are not easily cleaved. These noncleavable but tightly binding substrates are optionally structurally modified to yield inhibitory compounds with optimized properties. Additionally these substrates exhibit apparent specificity for a transition state or ground state configuration of the protease.

    Fluorescent pH indicators for intracellular assays
    108.
    发明公开
    Fluorescent pH indicators for intracellular assays 审中-公开
    Fluoreszierende PH IndikatorenfürintrazelluläreAssays

    公开(公告)号：EP1281712A1

    公开(公告)日：2003-02-05

    申请号：EP02255414.1

    申请日：2002-08-01

    申请人： Molecular Devices Corporation

    发明人： Diwu, Zhenjun ,  Twu, Jesse J. ,  Yi, Guoliang ,  Lavis, Luke D. ,  Chen, Yen-Wen ,  Cassutt, Kelly J.

    IPC分类号： C07D493/10 ,  C07D311/82 ,  G01N33/84 ,  C12Q1/44

    CPC分类号： C07D493/10 ,  C07D311/82 ,  G01N1/30 ,  G01N33/84 ,  G01N2001/302 ,  G01N2800/04

    摘要： Systems, including compositions and methods, for measuring pH, particularly in cells, organelles, and other samples. The compositions include pH-sensitive fluorescent and fluorogenic 2',7'-dialkylfluorescein derivatives and associated nonfluorescent precursor compounds of the formula shown below. The compositions may permit ratiometric measurement in the excitation spectrum and the emission spectrum. The methods include adding a precursor compound to a sample cell, incubating the sample cell to release the free indicator, illuminating the sample cell, and detecting the fluorescence response of the free indicator.

    摘要翻译： 用于测量pH的系统，包括组合物和方法，特别是在细胞，细胞器和其它样品中。 组合物包括pH敏感的荧光和荧光2'，7'-二烷基荧光素衍生物和下列所示式的相关非荧光前体化合物。 组合物可以允许在激发光谱和发射光谱中进行比例测量。 所述方法包括向样品池中加入前体化合物，孵育样品池以释放游离指示剂，照射样品池，以及检测游离指示剂的荧光响应。