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    METHOD OF PRODUCING OPTICALLY ACTIVE N-(HALOPROPYL)AMINO ACID DERIVATIVE
    131.
    发明公开
    METHOD OF PRODUCING OPTICALLY ACTIVE N-(HALOPROPYL)AMINO ACID DERIVATIVE 有权
    VERFAHREN ZUR HERSTELLUNG EINES OPTISCH AKTIVEN N-（HALOGENPROPYL）AMINOSÄUREDERIVATS

    公开(公告)号：EP2192110A1

    公开(公告)日：2010-06-02

    申请号：EP08753110.9

    申请日：2008-05-19

    申请人： Nagase & Co., Ltd.

    发明人： MATSUMOTO, Jun ,  INOUE, Toru

    IPC分类号： C07C231/02 ,  C07C227/14 ,  C07C227/30 ,  C07C229/12 ,  C07C233/83 ,  C07C269/04 ,  C07C271/22 ,  C07B53/00

    CPC分类号： C07B53/00 ,  C07B2200/07 ,  C07C229/12 ,  C07C233/83 ,  C07C271/22 ,  Y02P20/55

    摘要： The present invention provides a method for producing an optically active N-(halopropyl)amino acid derivative, wherein the method comprises the steps of obtaining a compound represented by formula (III) by reacting an optically active alanine ester represented by formula (I) or a salt thereof (hereinafter sometimes simply referred to as an "alanine ester") with a halogenated propane represented by formula (II); and obtaining an optically active N-(halopropyl)amino acid derivative represented by formula (IV) by introducing a protecting group onto the nitrogen atom of the compound represented by formula (III). The present invention provides a method for efficiently producing an optically active N-(halopropyl)amino acid derivative.

    摘要翻译： 本发明提供一种光学活性N-（卤代丙基）氨基酸衍生物的制造方法，其中，该方法包括以下步骤：通过使式（I）表示的光学活性丙氨酸酯或式 其盐（以下有时简称为“丙氨酸酯”）与式（II）表示的卤代丙烷; 通过在式（III）表示的化合物的氮原子上引入保护基，得到由式（Ⅳ）表示的光学活性的N-（卤代丙基）氨基酸衍生物。 本发明提供了有效制备光学活性N-（卤代丙基）氨基酸衍生物的方法。

    ISOLATION OF NUCLEIC ACIDS
    136.
    发明授权
    ISOLATION OF NUCLEIC ACIDS 有权
    核酸分离

    公开(公告)号：EP1345952B1

    公开(公告)日：2004-11-24

    申请号：EP01270542.2

    申请日：2001-12-14

    申请人： DNA Research Innovations Limited

    发明人： BAKER, Matthew, John

    IPC分类号： C07H1/08 ,  C07C229/12 ,  C07C233/47 ,  C07C229/16 ,  C07D295/08

    CPC分类号： C12N15/101 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1013

    摘要： The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, pH at which the charge on the material is negative, neutral or less positive. The release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.

    Isolation of nucleid acids
    137.
    发明公开
    Isolation of nucleid acids 审中-公开
    Nucleinsäure，隔离

    公开(公告)号：EP1473299A2

    公开(公告)日：2004-11-03

    申请号：EP04017291.8

    申请日：2001-12-14

    申请人： DNA Research Innovations Limited

    发明人： Baker, Matthew, John

    IPC分类号： C07H1/08 ,  C07C229/12 ,  C07C233/47 ,  C07C229/16 ,  C07D295/08

    CPC分类号： C12N15/101 ,  C12N15/1003 ,  C12N15/1006 ,  C12N15/1013

    摘要： The present invention relates to materials for extracting nucleic acid using charge switch methods which involve, at a first pH, bringing the sample into contact with a material which comprises an ionisable group, wherein the material has a positive charge at said first pH, such that nucleic acid is bound to the material; and releasing the nucleic acid at a second, higher, pH at which the charge on the material is negative, neutral or less positive. The release of the nucleic acid occurs under mild conditions and the ionisable group is provided by a biological buffer, a polyhydroxylated amine, a detergent or surfactant, a carbohydrate, a nucleic acid base, a heterocyclic nitrogen-containing compound, a monoamine, a biological dye, or a negatively ionisable group, the pKa of which is between about 3.0 and 7.0 and a metal oxide which is positively charged at said first pH, and optionally also at said second pH.

    摘要翻译： 本发明涉及使用电荷开关方法提取核酸的材料，该方法涉及在第一pH下使样品与包含可离子化基团的材料接触，其中所述材料在所述第一pH下具有正电荷，使得 核酸与材料结合; 并在第二个较高的pH下释放核酸，在该pH下，材料上的电荷是负的，中性的或较小的阳性。 核酸的释放在温和条件下发生，并且可电离基团由生物缓冲液，多羟基化胺，去污剂或表面活性剂，碳水化合物，核酸碱基，杂环含氮化合物，单胺，生物 染料或负电离基团，其pKa在约3.0和7.0之间，金属氧化物在所述第一pH和任选地还在所述第二pH下带正电荷。