公开(公告)号：EP2045335B1

公开(公告)日：2014-12-31

申请号：EP08021839.9

申请日：2006-04-17

申请人： Epigenomics AG

发明人： Distler, Jürgen ,  Hildmann, Thomas ,  Lesche, Ralf ,  Lofton-Day, Cathy ,  Model, Fabian ,  Schuster, Matthias ,  Sledziewski, Andrew ,  Song, Xiaoling ,  Tetzner, Reimo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q1/6806 ,  C12Q2600/106 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/158

公开(公告)号：EP1794319B1

公开(公告)日：2014-08-13

申请号：EP05802610.5

申请日：2005-09-30

申请人： Epigenomics AG

发明人： BALLHAUSE, Matthias ,  BERLIN, Kurt ,  DIETRICH, Dimo ,  KLUTH, Antje ,  SCHUSTER, Matthias ,  WAGNER, Ute ,  WASSERKORT, Reinhold ,  ZIEBARTH, Heike

IPC分类号： C12Q1/68 ,  C12N15/10

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q1/6858 ,  C12Q1/686 ,  C12Q2523/125 ,  C12Q2527/137 ,  C12Q2527/149 ,  C12Q2531/107

摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.

公开(公告)号：EP2634264A1

公开(公告)日：2013-09-04

申请号：EP13154538.6

申请日：2007-07-23

申请人： Epigenomics AG

发明人： Lofton-Day, Catherine ,  Sledziewski, Andrew ,  Model, Fabian ,  Cottrell, Susan ,  Distler, Jürgen ,  Tetzner, Reimo ,  Dietrich, Dimo

IPC分类号： C12Q1/68 ,  G01N33/574

CPC分类号： C12Q1/6886 ,  C12Q2600/154

摘要： The invention provides methods, nucleic acids and kits for detecting colorectal cell proliferative disorders based on the gene PCDHGC3. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

摘要翻译： 本发明提供了方法，核酸和试剂盒，用于检测基于所述基因PCDHGC3结肠细胞增殖性疾病。 本发明光盘松散的基因组序列哪甲基化模式对所述类疾病的改进的检测工具，从而使得患者的改进的诊断和治疗。

公开(公告)号：EP1945806B1

公开(公告)日：2013-02-27

申请号：EP06805804.9

申请日：2006-09-21

申请人： Epigenomics AG

发明人： FOEKENS, John ,  MARTENS, John ,  EPPENBERGER-CASTORI, Serenella ,  VUAROQUEAUX, Vincent ,  SPYRATOS, Frédérique ,  HARBECK, Nadia ,  SCHMITT, Manfred ,  HÖFLER, Heinz ,  MAIER, Sabine ,  WEISS, Gunter ,  LESCHE, Ralf ,  HILDMANN, Thomas ,  PLUM, Achim

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/106 ,  C12Q2600/118 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/16

公开(公告)号：EP2484778A3

公开(公告)日：2012-10-10

申请号：EP11009932.2

申请日：2007-04-17

申请人： Epigenomics AG

发明人： Lofton-Day, Catherine ,  Ebert, Matthias

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/158

摘要： The invention provides methods, nucleic acids and kits for detecting, or for distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

公开(公告)号：EP1871912B1

公开(公告)日：2012-02-29

申请号：EP06750657.6

申请日：2006-04-17

申请人： Epigenomics AG

发明人： BALLHAUSE, Matthias ,  BERLIN, Kurt ,  DEVOS, Theo ,  DIETRICH, Dimo ,  LIEBENBERG, Volker ,  LOFTON-DAY, Cathy ,  LOGRASSO, Joe ,  MAAS, Jennifer ,  MODEL, Fabian ,  SCHUSTER, Matthias ,  SLEDZIEWSKI, Andrew ,  TETZNER, Reimo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6883 ,  C12N15/1003 ,  C12Q1/6806 ,  C12Q2523/125 ,  C12Q2600/154 ,  Y10T436/143333 ,  C12Q2521/131 ,  C12Q2531/101

摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from a remote sample. In particular aspects a remote sample comprising DNA is provided, DNA is isolated from the remote sample, and the isolated DNA is treated in a way which allows differentiation of methylated and unmethylated cytosine. Additional, particular embodiments provide compositions and methods for methylation analysis of DNA derived from a remote sample. Other aspects provide for compositions and methods of whole genome amplification of bisulfite treated DNA.

公开(公告)号：EP2354249A1

公开(公告)日：2011-08-10

申请号：EP10174640.2

申请日：2004-06-23

申请人： Epigenomics AG

发明人： Lofton-Day, Cathy ,  Model, Fabian ,  Sledziewski, Andrew ,  Rujan, Tamas ,  Lewin, Jörn ,  Distler, Jürgen

IPC分类号： C12Q1/68 ,  C07H21/00

CPC分类号： C12Q1/6886 ,  C12Q2600/112 ,  C12Q2600/154 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Q2600/16

摘要： The invention provides methods, nucleic acids and kits for detecting, or for detecting and distinguishing between or among colorectal cell proliferative disorders. The invention discloses genomic sequences the methylation patterns of which have utility for the improved detection of and differentiation between said class of disorders, thereby enabling the improved diagnosis and treatment of patients.

摘要翻译： 本发明提供用于检测或检测和区分结肠直肠细胞增殖性疾病或其中的方法，核酸和试剂盒。 本发明公开了基因组序列，其甲基化模式可用于改善所述疾病类别的检测和分化，从而能够改善患者的诊断和治疗。

公开(公告)号：EP1481095B1

公开(公告)日：2010-12-08

申请号：EP03742964.4

申请日：2003-02-27

申请人： Epigenomics AG

发明人： ADORJAN, Peter ,  BURGER, Matthias ,  MAIER, Sabine ,  LESCHE, Ralf ,  COTTRELL, Susan ,  MOONEY, Suzanne

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6886 ,  C12Q2600/154 ,  Y10T436/143333

摘要： The present invention relates to modified and genomic sequences, to oligonucleotides and/or PNA-oligomers for detecting the cytosine methylation state of genomic DNA, as well as to a method for ascertaining genetic and/or epigenetic parameters of genes for use in the differentiation, diagnosis, treatment and/or monitoring of colon cell proliferative disorders, or the predisposition to colon cell proliferative disorders.

公开(公告)号：EP2189538A1

公开(公告)日：2010-05-26

申请号：EP08169662.7

申请日：2008-11-21

申请人： Epigenomics AG

发明人： Tetzner, Reimo

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6853 ,  C12Q2525/301 ,  C12Q2525/186 ,  C12Q2523/125

摘要： The invention relates to an analysis molecule for the analysis of one or more nucleotide positions in a nucleic acid. Said positions are in a first state or an alternative second state. The invention further relates to inventive methods that comprise the use of the inventive analysis molecule. The invention further relates to inventive kits that comprise the inventive analysis molecule.

摘要翻译： 本发明涉及用于分析核酸中一个或多个核苷酸位置的分析分子。 所述位置处于第一状态或替代的第二状态。 本发明还涉及包括使用本发明分析分子的本发明方法。 本发明还涉及包含本发明分析分子的本发明试剂盒。

公开(公告)号：EP1794319A4

公开(公告)日：2009-10-21

申请号：EP05802610

申请日：2005-09-30

申请人： EPIGENOMICS AG

发明人： BALLHAUSE MATTHIAS ,  BERLIN KURT ,  DIETRICH DIMO ,  KLUTH ANTJE ,  SCHUSTER MATTHIAS ,  WAGNER UTE ,  WASSERKORT REINHOLD ,  ZIEBARTH HEIKE

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6806 ,  C12N15/1003 ,  C12Q2527/127 ,  C12Q2523/125 ,  C12Q2521/531 ,  C12Q2547/107

摘要： Aspects of the present invention relate to compositions and methods for providing DNA fragments from an archived sample (e.g., paraffin-embedded and/or fixed-tissue biopsies, etc). Particular aspects provide methods whereby high yields of DNA are isolated as well as a substantial portion of the DNA consists of long DNA fragments, and where the isolated genomic DNA is free of associated or cross-linked contaminants like proteins, peptides, amino acids or RNA. The methods are facile, cost-effective, an are characterized by high reproducibility and reliability. Particular aspects provide methods for providing DNA fragments derived from an archived sample, wherein the yield of DNA before, for example, an amplification step is at least 20%, and amplicons up to a length of about 1,000 base pairs are amplifiable.