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    DETERMINATION METHOD FOR ALLERGIC DISEASE
    11.
    发明公开
    DETERMINATION METHOD FOR ALLERGIC DISEASE 有权
    BESTIMMUNGSVERFAHRENFÜREINE ALLERGISCHE ERKRANKUNG

    公开(公告)号：EP2136211A1

    公开(公告)日：2009-12-23

    申请号：EP08710396.6

    申请日：2008-02-15

    申请人： The University of Tokushima ,  Oriental Yeast Co., Ltd.

    发明人： KIDO, Hiroshi ,  SUZUKI, Koichi

    IPC分类号： G01N33/53 ,  G01N21/78 ,  G01N33/531 ,  G01N33/543

    CPC分类号： G01N33/6854 ,  G01N33/54373

    摘要： It is to provide a method for determining allergic diseases with a high sensitivity and accuracy which enables multilateral and global analysis with a minute amount of sample, even using body fluid other than blood such as saliva, nasal discharge and tears as a sample, particularly by suppressing nonspecific reaction as much as possible. A chemically modified diamond/DLC (Diamond-like Carbon) chip is activated with a reacting reagent, a coupling reaction with a peptide comprising allergen or allergen epitope is conducted, and a sample such as saliva, tears, and nasal discharge which has undergone pressure filtration with a low protein-adsorbing filter, is contacted with an allergen determination chip to which washing and blocking operations have been performed on unreacted active groups, and an allergen recognizing antibody in the sample captured by the allergen determination chip is detected by immunoassay using a labeled secondary antibody, wherein a glycine-containing solution is used for a washing solution and/or blocking solution used in the washing and blocking operations, is used.

    摘要翻译： 它是提供一种以高灵敏度和精确度确定过敏性疾病的方法，其能够使用少量样品进行多边和全球分析，甚至使用血液以外的体液（如唾液，鼻腔排泄物和泪液）作为样品，特别是通过 尽可能地抑制非特异性反应。 用反应试剂激活化学改性的金刚石/ DLC（类金刚石碳）芯片，进行与包含变应原或变应原表位的肽的偶联反应，以及经历压力的样品例如唾液，泪液和鼻放电 与低蛋白质吸附过滤器的过滤与已对未反应的活性基团进行洗涤和封闭操作的变应原测定芯片接触，并且通过免疫测定法检测由变应原测定芯片捕获的样品中的变应原识别抗体，使用 使用其中含有甘氨酸的溶液用于洗涤和阻塞操作中使用的洗涤溶液和/或封闭溶液。

    METHOD OF DETECTING MAMMARY CANCER CELLS
    12.
    发明公开
    METHOD OF DETECTING MAMMARY CANCER CELLS 审中-公开
    VERFAHREN ZUM NACHWEIS VON BRUSTKREBSZELLEN

    公开(公告)号：EP2128621A1

    公开(公告)日：2009-12-02

    申请号：EP07707909.3

    申请日：2007-02-01

    申请人： Oriental Yeast Co., Ltd.

    发明人： KINUGASA, Masahiro ,  SUGIMOTO, Michiyo ,  UCHIDA, Koji

    IPC分类号： G01N33/574

    CPC分类号： G01N33/57415

    摘要： The present invention provides a novel method for detecting mammary cancer cells which uses, as an index, an expression level of a specific gene in human mammary cancer tissue (or cells). The method is characterized by including (1) measuring an expression level of a gene having a specific nucleotide sequence in human mammary cancer tissue (or cells), (2) measuring an expression level of the gene in human normal mammary gland tissue (or cells), and (3) detecting mammary cancer cells on the basis of the difference between measurement values obtained in (1) and (2).

    摘要翻译： 本发明提供了一种用于检测乳腺癌细胞的新方法，其以人乳腺癌组织（或细胞）中特定基因的表达水平作为指标。 该方法的特征在于：（1）测定人乳腺癌组织（或细胞）中具有特定碱基序列的基因的表达水平，（2）测定人正常乳腺组织（或细胞）中基因的表达水平 ），（3）基于（1）和（2）中得到的测定值之差来检测乳腺癌细胞。

    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT
    13.
    发明公开
    METHOD OF SEPARATING AND DISTINGUISHING WALNUT FROM PECAN NUT 审中-公开
    维多利亚州立大学维多利亚州立大学

    公开(公告)号：EP2110433A1

    公开(公告)日：2009-10-21

    申请号：EP08710648.0

    申请日：2008-01-30

    申请人： Oriental Yeast Co., Ltd. ,  Nissin Foods Holdings Co., Ltd.

    发明人： YANO, Takeo ,  SAKAI, Yumiko ,  UCHIDA, Kouji ,  NAKAO, Yoshiki ,  ISHIHATA, Kimie ,  NAKANO, Shigeru ,  YAMADA, Toshihiro

    IPC分类号： C12N15/00 ,  C12N15/09 ,  C12Q1/68

    CPC分类号： C12Q1/6895 ,  A01H1/04 ,  C07K14/415

    摘要： [Object] To newly develop a method whereby walnut can be simply, rapidly and accurately separated and distinguished from pecan nut.
    [Means for Resolution] Due to the increase in the number of patients with the walnut allergen and the increase of import of pecan nut which is an edible nut belonging to the family Juglandaceae, recently in Japan, the development of a method for detecting walnut and the nut in a discriminative manner is urgently needed. With attention focused on the chloroplast matK gene with the apparent gene sequence, we developed a PCR method for specifically detecting nuts of the family Juglandaceae and identified the matK sequence of pecan nut to establish a method for discriminating walnut and pecan nut from each other using restriction enzymes, for discriminating walnut and pecan nut from each other.

    摘要翻译： [对象]新开发一种核桃可以简单，快速，准确地分离和区别于山核桃坚果的方法。 [解决方法]由于核桃过敏原患者数量的增加以及日本最近在日本的菊科植物中属于可食用坚果的山核桃进口量的增加，因此开发了检测核桃的方法 迫切需要有歧视的手段。 重点关注具有表观基因序列的叶绿体matK基因，我们开发了一种特异性检测菊科植物坚果的PCR方法，并鉴定了山核桃坚果的matK序列，建立了一种用核桃和山核桃果实进行限制的方法 用于区分核桃和山核桃果仁的酶。