公开(公告)号：EP1418180A2

公开(公告)日：2004-05-12

申请号：EP02760073.3

申请日：2002-07-12

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical 'Pedrokouri'

发明人： HERMIDA CRUZ, Lisset ,  RODRIGUEZ DIAZ, Rayner; ,  LAZO VAZQUEZ, Laura ,  ZULUETA MORALES, Aida ,  LOPEZ ABARRATEGUI, Carlos ,  VALDES PRADO, Iris ,  SILVA RODRIGUEZ, Ricardo de la C. ,  CHINEA SANTIAGO, Glay ,  GUILLEN NIETO, Gerardo Enrique ,  GUZMAN TIRADO, Maria Guadalupe ,  SIERRA VAZQUEZ, Beatriz de la Caridad ,  ESPINOSA PEREZ, Raul Rafael

IPC分类号： C07K14/18 ,  C12N15/40 ,  C12N15/70 ,  A61K38/16 ,  C12Q1/68

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C12N9/0006 ,  C12N2770/24122 ,  Y02A50/386

摘要： The present invention is related to the obtaining of chimeric chains coding for proteins capable of inducing, in the recipient, a serotype-specific and protective humoral immune response against the infection by the Dengue virus, thus eliminating the effects of the serotype-nonespecific viral immunoenhancement that causes hemorrhagies and clinical complications described for this kind of pathology. These chimeric chains of nucleic acids are composed by the specific combination of fragments belonging to the gene of a mutated protein from Neisseria meningitidis with dehydrogenase activity and fragments that codify for a region of the envelope (E) protein from the Dengue virus which, when inserted to an expression vector, give rise to chimeric proteins with particular properties. The resultant chimeric molecules from this invention are applicable to the pharmaceutical industry for the obtaining of vaccine preparations and diagnostic means of high serotype-specificity to be used in humans.

摘要翻译： 本发明涉及获得编码能够在受体中诱导针对登革热病毒感染的血清型特异性和保护性体液免疫应答的蛋白质的嵌合链，从而消除了血清型非特异性病毒免疫增强的作用 导致这种病理描述的出血和临床并发症。 核酸的这些嵌合链由来自脑膜炎奈瑟氏球菌与脱氢酶活性的突变蛋白的基因的片段的特异性组合组成，并且编码来自登革病毒的包膜（E）蛋白的区域的片段，其在插入时 表达载体，产生具有特定性质的嵌合蛋白。 所得到的本发明的嵌合分子适用于获得用于人类的疫苗制剂和高血清型特异性的诊断方法的制药工业。

公开(公告)号：EP0966535A1

公开(公告)日：1999-12-29

申请号：EP98900843.0

申请日：1998-01-13

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical "Pedro Kouri" IPK

发明人： VAZQUEZ RAMUDO, Susana ,  GUZMAN TIRADO, Guadalupe ,  GUILLEN NIETO, Gerardo Enrique ,  PARDO LAZO, Orlando Luis ,  CHINEA SANTIAGO, Glay ,  PEREZ DIAZ, Ana Beatriz ,  PUPO ANTUNEZ, Maritza ,  RODRIGUEZ ROCHE, Rosmari ,  REYES ACOSTA, Osvaldo ,  GARAY PEREZ, Hilda Elisa ,  PADRON PALOMARES, Gabriel ,  ALVAREZ VERA, Maylin ,  MORIER DIAZ, Luis ,  PEREZ INSUITA, Omaida ,  PELEGRINO MARTINEZ DE LA COTERA, José Luis

IPC分类号： C12N15 ,  A61K38 ,  A61K39 ,  A61P31 ,  C07K14 ,  C07K16

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24122 ,  G01N2333/18 ,  Y02A50/386

摘要： The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.

公开(公告)号：EP2690107A2

公开(公告)日：2014-01-29

申请号：EP12716190.9

申请日：2012-03-21

申请人： Centro De Ingenieria Genetica Y Biotecnologia

发明人： ABRAHANTES PÉREZ, María, del Carmen ,  CHINEA SANTIAGO, Glay ,  MARTÍNEZ DÍAZ, Eduardo ,  GARAY PÉREZ, Hilda, Elisa ,  REYES ACOSTA, Osvaldo ,  LÓPEZ MOLA, Ernesto ,  LÓPEZ ABAD, Cruz, Matilde ,  GONZALEZ BLANCO, Sonia

IPC分类号： C07K7/06

CPC分类号： A61K38/12 ,  A61K9/0019 ,  A61K9/127 ,  A61K9/5031 ,  A61K31/4025 ,  A61K38/00 ,  A61K45/06 ,  A61K47/60 ,  C07K7/06 ,  C07K7/08 ,  C07K7/64

摘要： The present invention comprises cyclic peptides bearing antitumor and antiangiogenic properties, as well as their corresponding pharmaceutically-suitable salts and also pharmaceutical compositions containing it. These cyclic peptides are used to prepare medicines for human and/or veterinary therapeutics, and additionally in diagnosis. These compounds can be used to detect, monitor and/or control a range of cellular proliferation-related disorders, such as oncological diseases and undesired angiogenesis. Moreover, they can be included as part of controlled release systems, and used more precisely in the field of nanobiotechnology, either because of their self-assembly capacity or as part of other systems.

摘要翻译： 本发明包括具有抗肿瘤和抗血管生成特性的环肽，以及它们相应的药学上合适的盐以及含有它的药物组合物。 这些环肽用于制备用于人和/或兽医治疗剂的药物，另外用于诊断。 这些化合物可用于检测，监测和/或控制一系列细胞增殖相关疾病，例如肿瘤疾病和不期望的血管生成。 此外，它们可以作为控制释放系统的一部分纳入纳米生物技术领域，或者由于其自组装能力或作为其他系统的一部分。

公开(公告)号：EP2085098B1

公开(公告)日：2012-07-25

申请号：EP07817384.6

申请日：2007-10-30

申请人： Centro De Ingenieria Genetica Y Biotecnologia

发明人： CHINEA SANTIAGO, Glay ,  HUERTA GALINDO, Vivian ,  MARTÍN DUNN, Alejandro Miguel ,  FLEITAS SALAZAR, Noralvis ,  GUIROLA CRUZ, Osmany ,  TOLEDO MAYORA, Patricia, Gabriela ,  SARRÍA NÚÑEZ, Mónica ,  MUSACCHIO LASA, Alexis ,  REYES ACOSTA, Osvaldo ,  GARAY PÉREZ, Hilda Elisa ,  CABRALES RICO, Ania

IPC分类号： C07K19/00

CPC分类号： A61K47/48315 ,  A61K38/00 ,  A61K47/645 ,  C07K14/005 ,  C07K2319/04 ,  C07K2319/10 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24222 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/389 ,  Y02A50/393 ,  Y02A50/395

摘要： The present invention is relative to chimerical peptides, whose primary structure holds at least one segment which inhibits the activation of the NS3 protease of a virus from the Flaviviridae family, they also contain a cell penetrating segment and they are capable of inhibiting or attenuate the viral infection. This invention is also relative to pharmaceutical compounds which contain these chimerical peptides for the prevention and/or treatment of the infection caused by a virus of the Flaviviridae family.

摘要翻译： 本发明是相对于嵌合肽，其主要结构保持至少一个片段，其阻止来自黄病毒科的病毒的NS3蛋白酶的活化，它们还含有细胞穿透片段，并且它们能够抑制或减弱病毒 感染。 本发明还涉及含有这些嵌合肽用于预防和/或治疗由黄病毒科病毒引起的感染的药物化合物。

公开(公告)号：EP2258356A2

公开(公告)日：2010-12-08

申请号：EP09714439.8

申请日：2009-02-27

申请人： Centro De Ingenieria Genetica Y Biotecnologia

发明人： MAZOLA REYES, Yuliet ,  CHINEA SANTIAGO, Glay ,  GUIROLA CRUZ, Osmany ,  VERA ALVAREZ, Roberto ,  HUERTA GALINDO, Vivian ,  FLEITAS SALAZAR, Noralvis ,  MUSACCHIO LASA, Alexis

IPC分类号： A61K31/00 ,  A61K31/496 ,  A61K31/4453 ,  A61K31/5375 ,  A61K31/5377 ,  A61K31/425 ,  A61K31/381 ,  A61K31/404 ,  A61K31/433 ,  A61K31/40 ,  A61K31/4192 ,  A61K31/34 ,  A61K31/505 ,  A61K31/55 ,  A61P31/14

CPC分类号： A61K31/16 ,  A61K31/18 ,  A61K31/381 ,  A61K31/41 ,  A61K31/426 ,  A61K31/428 ,  A61K31/44 ,  A61K31/495 ,  A61K31/4965 ,  A61K31/50 ,  A61K31/506 ,  A61K31/535 ,  A61K31/55 ,  A61K47/54 ,  Y02A50/385 ,  Y02A50/387 ,  Y02A50/389 ,  Y02A50/393 ,  Y02A50/395

摘要： Use of chemical compounds obtained in silico for the preparation of pharmaceutical compositions to attenuate or inhibit Dengue virus infection. Particularly, through the interference or the modulation of several stages of viral replication cycle related with the entry of virus into host cells and the assembly of mature progeny virions. The invention also comprises the use of such pharmaceutical compositions for prophylactic and/or therapeutic treatment of infection caused by all four serotypes of Dengue virus and other flaviviruses.

摘要翻译： 使用在硅胶中获得的化合物用于制备药物组合物以减轻或抑制登革热病毒感染。 特别是通过与病毒进入宿主细胞相关的几个阶段的病毒复制循环的干扰或调节以及成熟后代病毒粒子的组装。 本发明还包括使用这种药物组合物预防和/或治疗由登革热病毒和其他黄病毒的所有四种血清型引起的感染。

公开(公告)号：EP1982994A2

公开(公告)日：2008-10-22

申请号：EP06828471.0

申请日：2006-12-28

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA

发明人： MENÉNDEZ MEDINA, Tamara ,  CRUZ LEAL, Yoelys ,  REYES ACOSTA, Osvaldo ,  GARAY PÉREZ, Hilda, Elisa ,  GUILLÉN NIETO, Gerardo, Enrique ,  COIZEAU RODRÍGUEZ, Edelgis ,  SANTIAGO VISPO, Nelson, Francisco ,  CHINEA SANTIAGO, Glay

IPC分类号： C07K14/22 ,  A61K38/16 ,  G01N33/68

CPC分类号： C07K14/22 ,  A61K39/00

摘要： The present invention is related with the pharmaceutical industry and the biotechnology, specifically with vaccine antigens applied against bacterial, viral, cancerous or another origin diseases. Development of pharmaceuticals formulations able to protect or to increase the protective spectrum of already developed vaccines and to extend it against several pathogens, using as target for the immune response, carbohydrates composed of repeated units of (α 2-8) linked N-replaced neuraminic acid. Several peptides were isolated and identified as molecular mimetics of the capsular polysaccharide form serogroup B of Neisseria meningitidis, which is composed of repeated units of (α 2-8) linked N-acetyl neuraminic acid. The immunogenicity of peptides was evaluated in animal biomodels, demonstrating their value as antigens able to induce the production of antibodies able of specifically recognize the bacterium N. meningitidis from serogroup B and of showing bactericidal activity against the same one. The resulting formulations of are applicable in the pharmaceutical industry as vaccine formulations for human use.

摘要翻译： 本发明涉及制药工业和生物技术，特别是针对细菌，病毒，癌性或其它起源疾病的疫苗抗原。 开发能够保护或增加已经开发的疫苗的保护范围并将其扩展到几种病原体的药物制剂，其用作免疫应答的靶标，由（±2-8）连接的N-取代的神经氨酸的重复单位组成的碳水化合物 酸。 分离出几种肽，鉴定为脑膜炎奈瑟菌脑膜炎血清群B的荚膜多糖的分子模拟物，其由重复单位的（±2-8）连接的N-乙酰神经氨酸组成。 在动物生物模型中评估肽的免疫原性，证明其作为能够诱导产生能够特异性识别来自血清群B的脑膜炎奈瑟菌细菌的抗体的抗原的值，并且对相同抗体具有杀菌活性。 所得到的制剂可用于制药工业，作为用于人类的疫苗制剂。

公开(公告)号：EP1958959A2

公开(公告)日：2008-08-20

申请号：EP06817996.9

申请日：2006-11-21

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB)

发明人： CHINEA SANTIAGO, Glay ,  HUERTA GALINDO, Vivian ,  MARTÍN DUNN, Alejandro, Miguel ,  GAVILONDO COWLEY, Jorge, Victor ,  FLEITAS SALAZAR, Noralvis ,  GUIROLA CRUZ, Osmany ,  GIL VALDÉS, Jeovanis ,  ZULUETA MORALES, Aída ,  HERMIDA CRUZ, Lisset ,  AYALA AVILA, Marta ,  GONZÁLEZ ROCHE, Diamilé ,  PAEZ MEIRELES, Rolando ,  TOLEDO MAYORA, Patricia, Gabriela ,  SARRÍA NÚÑEZ, Mónica ,  MUSACCHIO LASA, Alexis ,  MAZOLA REYES, Yuliet

IPC分类号： C07K14/18 ,  C07K19/00 ,  C07K16/10 ,  C12N15/40 ,  C12N15/62 ,  C12N15/63 ,  A61K39/12

CPC分类号： C07K14/005 ,  A61K39/00 ,  A61K39/12 ,  A61K2039/55566 ,  C07K2319/02 ,  C07K2319/21 ,  C12N2770/24022 ,  C12N2770/24122 ,  C12N2770/24134 ,  Y02A50/386 ,  Y02A50/388 ,  Y02A50/39 ,  Y02A50/394 ,  Y02A50/396

摘要： The present invention is related to the field of the pharmaceutical industry, and describes a conserved area on the surface of the E protein that can be used for the development of wide-spectrum antiviral molecules to be employed in the prophylaxis and/or treatment of infections due to Dengue Virus serotypes 1-4 and other flaviviruses. The invention also covers chimeric proteins to be used as vaccines or as a prophylactic or therapeutic treatment against the four serotypes of Dengue Virus and other flaviviruses.

摘要翻译： 本发明涉及制药工业领域，并且描述了E蛋白的表面上的一个保守的区域也可用于广谱抗病毒分子的发展感染的预防和/或治疗要采用 由于登革热病毒血清型1-4和其他黄病毒。 因此，本发明涵盖了嵌合蛋白用作疫苗或作为针对登革病毒和其他黄病毒的四种血清型的预防或治疗性治疗。

公开(公告)号：EP1418180B1

公开(公告)日：2006-12-27

申请号：EP02760073.3

申请日：2002-07-12

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical 'Pedrokouri'

发明人： HERMIDA CRUZ, Lisset ,  RODRIGUEZ DIAZ, Rayner; ,  LAZO VAZQUEZ, Laura ,  ZULUETA MORALES, Aida ,  LOPEZ ABARRATEGUI, Carlos ,  VALDES PRADO, Iris ,  SILVA RODRIGUEZ, Ricardo de la C. ,  CHINEA SANTIAGO, Glay ,  GUILLEN NIETO, Gerardo Enrique ,  GUZMAN TIRADO, Maria Guadalupe ,  SIERRA VAZQUEZ, Beatriz de la Caridad ,  ESPINOSA PEREZ, Raul Rafael

IPC分类号： C07K14/18 ,  C12N15/40 ,  C12N15/70 ,  A61K38/16 ,  C12Q1/68

CPC分类号： C07K14/005 ,  A61K39/00 ,  C07K2319/00 ,  C12N9/0006 ,  C12N2770/24122 ,  Y02A50/386

摘要： The present invention is related to the obtaining of chimeric chains coding for proteins capable of inducing, in the recipient, a serotype-specific and protective humoral immune response against the infection by the Dengue virus, thus eliminating the effects of the serotype-nonespecific viral immunoenhancement that causes hemorrhagies and clinical complications described for this kind of pathology. These chimeric chains of nucleic acids are composed by the specific combination of fragments belonging to the gene of a mutated protein from Neisseria meningitidis with dehydrogenase activity and fragments that codify for a region of the envelope (E) protein from the Dengue virus which, when inserted to an expression vector, give rise to chimeric proteins with particular properties. The resultant chimeric molecules from this invention are applicable to the pharmaceutical industry for the obtaining of vaccine preparations and diagnostic means of high serotype-specificity to be used in humans.

公开(公告)号：EP0966535B1

公开(公告)日：2004-12-08

申请号：EP98900843.8

申请日：1998-01-13

申请人： CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) ,  Instituto de Medicina Tropical "Pedro Kouri" IPK

发明人： VAZQUEZ RAMUDO, Susana ,  GUZMAN TIRADO, Guadalupe ,  GUILLEN NIETO, Gerardo Enrique ,  PARDO LAZO, Orlando Luis ,  CHINEA SANTIAGO, Glay ,  PEREZ DIAZ, Ana Beatriz ,  PUPO ANTUNEZ, Maritza ,  RODRIGUEZ ROCHE, Rosmari ,  REYES ACOSTA, Osvaldo ,  GARAY PEREZ, Hilda Elisa ,  PADRON PALOMARES, Gabriel ,  ALVAREZ VERA, Maylin ,  MORIER DIAZ, Luis ,  PEREZ INSUITA, Omaida ,  PELEGRINO MARTINEZ DE LA COTERA, José Luis

IPC分类号： C12N15/40 ,  C07K14/18 ,  A61K39/12 ,  C07K16/10

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C12N2770/24122 ,  G01N2333/18 ,  Y02A50/386

摘要： The present invention relates to five synthetic peptides of pre-M/M protein of Dengue-2 virus, corresponding to amino acid sequences 3-31, 45-67, 57-92, 69-93 and 103-124. The anti-peptide immune response was evaluated in mice. Recombinant fusion proteins were also obtained, including regions of pre-M/M protein. The presence of B cell epitopes in both mice and humans was demonstrated in the pre-M/M protein peptides. Peptides 3-31 and 103-124 elicited neutralizing antibodies against the four serotypes of Dengue virus. Virus-specific proliferative responses were demonstrated in mice immunized with non-conjugated peptides 3-31 and 57-92. Mice immunized with conjugated peptides 3-31, 57-92, and 69-93 were protected when they were challenged with Dengue-2 virus. Thus, the presence of sequential epitopes in Pre-M/M protein of Dengue-2 virus was demonstrated, as well as their relevance in the immune response against this flavivirus.