摘要:
L'invention concerne un procédé de perfusion micro-fluidique d'un sphéroïde, mis en œuvre dans un dispositif micro-fluidique qui comporte une cavité (151) de piégeage hydrodynamique dudit sphéroïde, ledit procédé comportant les étapes suivantes : - Première injection d'un gel (20) contenant ledit sphéroïde dans le réseau micro-fluidique (10), - Piégeage hydrodynamique dudit sphéroïde dans la cavité (151) de piégeage du réseau micro-fluidique, - Deuxième injection d'un fluide non miscible avec ledit gel dans ledit réseau micro-fluidique pour chasser le gel présent dans le réseau, à l'exception de la cavité (151) de piégeage, - Réticulation du gel (20) présent autour du sphéroïde, dans la cavité (151) de piégeage, - Troisième injection d'un milieu de culture (22) dans ledit réseau micro-fluidique pour perfuser le sphéroïde fixé dans son environnement gélifié, localisé dans la cavité (151) de piégeage.
摘要:
L'invention concerne un procédé de fabrication d'un dispositif micro-fluidique (1) comportant un réseau micro-fluidique comprenant plusieurs capsules micro-fluidiques (3), chaque capsule micro-fluidique (3) étant réalisée par les étapes suivantes : - Réalisation d'une cavité (7) à travers une plaque support ; - Remplissage au moins partiel de ladite cavité (7) à l'aide d'un élément (31) en matériau déformable se présentant dans un état initial liquide ; - Solidification dudit élément (31) en matériau déformable à l'intérieur de la cavité (7) de manière à y délimiter une chambre de volume modulable par déformation dudit élément.
摘要:
The invention relates to a system for delivering a liquid, comprising: - a peristaltic pump (1) operating in cycles so that an elementary volume of liquid is delivered into the pipe during the course of each cycle, - at least one actuator (51 1 , 51 2 , 51 3 ) able to cause the pump to deliver a volume of liquid, during the course of a cycle, - a control circuit (55, 56, 58, 62), for applying a control signal to the actuator so that the volume delivered during the course of a cycle is dependent on this signal applied to the actuator, - a sensor (54, 60), able to produce a signal indicative of the quantity of liquid ejected during the course of a cycle, - the control circuit (55, 56, 58, 62) being able to determine a control signal on the basis of said signal from the sensor and of a reference signal (V D , V S ).
摘要:
The invention relates to a device or pipette for extracting a particle-depleted liquid phase from a suspension, comprising: - a main flow conduit (3) for said suspension, the main conduit comprising a fractionating cell (7) comprising at least one singularity location suitable for causing the formation of at least one recirculation vortex, and a clear layer pre-configuration channel (5) positioned upstream from the fractionating cell (7), said fractionating cell (7) and said pre-configuration channel (5) are formed on a same substrate, and - at least one liquid withdrawal means (15), positioned at a region of the fractionating cell (7) where said suspension is depleted in terms of particles due to said recirculation vortex.
摘要:
The invention relates to a micropump having a deformable membrane, comprising: a first chamber (15), one wall of which comprises a first deformable membrane portion and means (72, 81, 83) for actuating said first membrane portion; a second chamber (11) provided with a second deformable membrane portion (11'); and a third chamber (13) provided with a third deformable membrane portion (13'), the second chamber and the third chamber being connected to one another by a first channel (12), at least one of said chambers being connected to the first chamber (15) by a second channel (12, 14), wherein both the second chamber (11) and the third chamber (13) comprise detection gauge means (R1-R4, R'1-R'4), respectively, but are not provided with actuation means.
摘要:
A method for producing at least one deformable membrane micropump including a first substrate and a second substrate assembled together, the first substrate including at least one cavity and the second substrate including at least one deformable membrane arranged facing the cavity. In the method: the cavity is produced in the first substrate; then the first and second substrates are assembled together; then the deformable membrane is produced in the second substrate.
摘要:
The invention relates to a microfluidic device, comprising a microfluidic array, comprising: a) two parallel plates (2, 4) each equipped with one or more electrodes (3, 13), b) at least one channel (9), positioned between the two plates, made from a material obtained by solidification or hardening of the material of a first fluid (6), c) means for varying a physical parameter of the constituent material of the walls of the channel in order to change it at least from the liquid state to the solid state.