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    REAGENT COMPOSITIONS FOR ASSAYING ELECTROLYTES
    12.
    发明公开
    REAGENT COMPOSITIONS FOR ASSAYING ELECTROLYTES 有权
    珍珠棉

    公开(公告)号：EP0989189A4

    公开(公告)日：2004-10-13

    申请号：EP99907916

    申请日：1999-03-11

    申请人： TOYO BOSEKI

    发明人： KIMATA SHINSUKE ,  ASANO SHIGEKI ,  KAWAMURA YOSHIHISA

    IPC分类号： C12Q1/40 ,  G01N31/22 ,  G01N33/84

    CPC分类号： G01N33/84 ,  C12Q1/40 ,  C12Q2334/00 ,  C12Q2334/10 ,  G01N31/22 ,  G01N2400/18 ,  G01N2800/04

    摘要： Reagent compositions for enzymatically assaying electrolytes (calcium ion, chlorine ion, etc.) which withstand as liquid reagents during distribution, have a high solution stability and are excellent in quantitative characteristics and accuracy. These compositions comprises: (a) inactivated alpha -amylase; (b) a chelating agent; (c) an alpha -amylase substrate; and (d) a cyclodextrin derivative; optionally together with an SH-group containing compound or its salt.

    摘要翻译： 提供了用于电解质酶测定的试剂组合物，其具有高的溶液稳定性，使其能够在液体试剂中作为其在市场上的分布，并且还具有优异的定量可靠性和精度。 这是用于测定电解质如钙或氯离子的试剂组合物，其包含：（a）无活性的α-淀粉酶; （b）螯合剂; （c）α-淀粉酶底物; 和（d）环糊精衍生物，并且可以任选地进一步含有含SH基团的化合物或其盐。

    METHOD FOR ANALYZING INTRACELLULAR COMPONENTS
    13.
    发明公开
    METHOD FOR ANALYZING INTRACELLULAR COMPONENTS 有权
    VERFAHREN ZUR ANALYZEINTRAZELLULÄRERSUBSTANZEN

    公开(公告)号：EP1048739A4

    公开(公告)日：2004-06-30

    申请号：EP98956015

    申请日：1998-12-01

    申请人： KIKKOMAN CORP

    发明人： HATTORI NORIAKI ,  YAJITATE KEIKO ,  NAKAJIMA MOTOO ,  MURAKAMI SEIJI

    IPC分类号： G01N33/48 ,  C12Q1/06 ,  C12Q1/66 ,  C12Q1/68 ,  G01N33/66

    CPC分类号： G01N33/66 ,  C12Q1/66 ,  C12Q2304/60 ,  G01N2400/16 ,  G01N2400/18

    摘要： A method for analyzing intracellular components characterized by involving the following steps; and kits containing (a) an extractant, (b) a branched dextrin or its derivative, and (c) a reagent for analyzing intracellular components: (1) the step of adding the extractant to a sample containing cells to thereby extract intracellular components; (2) the step of adding the branched dextrin or its derivative to the sample containing the extractant; and (3) the step of analyzing the thus extracted intracellular components. The use of the branched dextrin or its derivative makes it possible to prevent the extractant from inhibiting the analysis steps.

    摘要翻译： 一种分析细胞内成分的方法，其特征在于包括以下步骤： 和（a）提取剂，（b）支链糊精或其衍生物的试剂盒和（c）用于分析细胞内成分的试剂：（1）将提取剂加入到含有细胞的样品中从而提取细胞内成分的步骤; （2）将分支糊精或其衍生物加入含有萃取剂的样品的步骤; 和（3）分析由此提取的细胞内组分的步骤。 使用分支糊精或其衍生物可以防止萃取剂抑制分析步骤。

    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS
    16.
    发明公开
    PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS 审中-公开
    蛋白质检测使用改性环糊精

    公开(公告)号：EP2986646A4

    公开(公告)日：2017-04-12

    申请号：EP14834424

    申请日：2014-08-08

    申请人： BIO-RAD LABORATORIES INC

    发明人： MCKEE CLAYTON T ,  STRONG WILLIAM ,  OLECH LEE ,  BERKELMAN THOMAS ,  BELISLE CHRISTOPHER

    IPC分类号： G01N33/58 ,  G01N27/447 ,  G01N33/68

    CPC分类号： G01N33/5306 ,  G01N27/44747 ,  G01N33/582 ,  G01N33/6803 ,  G01N2400/18

    摘要： A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins.

    HEMOLYTIC STREPTOCOCCUS DIAGNOSTIC IMMUNOCHROMATO REAGENT, KIT, AND DETECTION METHOD
    17.
    发明公开
    HEMOLYTIC STREPTOCOCCUS DIAGNOSTIC IMMUNOCHROMATO REAGENT, KIT, AND DETECTION METHOD 有权
    诊断诊断STREPTOCOCCUS-IMMUNOCHROMATOREAGENS，KIT UND NACHWEISVERFAHREN

    公开(公告)号：EP3032260A4

    公开(公告)日：2017-03-22

    申请号：EP14834355

    申请日：2014-08-08

    申请人： TANAKA PRECIOUS METAL IND

    发明人： KATO YUYA ,  IWAMOTO HISAHIKO

    IPC分类号： G01N33/53 ,  G01N33/543 ,  G01N33/558 ,  G01N33/569

    CPC分类号： G01N33/56944 ,  G01N33/5306 ,  G01N33/54393 ,  G01N33/558 ,  G01N2400/10 ,  G01N2400/18 ,  G01N2469/10

    摘要： Disclosed herein is an immunoassay method for detecting the detection target antigen in an analyte through extraction with an extraction agent. The method is characterized in that the detection is performed in the presence of a cyclic oligosaccharide. The method is also characterized in the use of an immunochromatography kit for detecting gram-positive bacteria in an analyte, and that is configured from an analyte dilution solution, a sample dropping part, an antigen extracting part, a labeling substance retaining part, an immunochromatography medium having a detection part, and an absorption part, and in which an organic acid is retained in the antigen extracting part. The immunochromatography kit contains a cyclic oligosaccharide and a nitrite compound by containing at least one of a cyclic oligosaccharide, a nitrite compound, and a mixture thereof in the analyte dilution solution and/or the sample dropping part.

    摘要翻译： 本文公开了一种通过用提取剂提取来检测分析物中的检测目标抗原的免疫测定方法。 该方法的特征在于在环状寡糖的存在下进行检测。 该方法的特征还在于使用免疫色谱试剂盒检测分析物中的革兰氏阳性细菌，并且由分析物稀释溶液，样品滴下部分，抗原提取部分，标记物质保留部分，免疫色谱法 具有检测部分的介质和吸收部分，其中有机酸保留在抗原提取部分中。 免疫色谱试剂盒含有环状低聚糖和亚硝酸盐化合物，在分析物稀释溶液和/或试样滴加部中含有环状低聚糖，亚硝酸盐化合物及其混合物中的至少一种。