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    ANALYTICAL COMPOSITION AND METHOD
    11.
    发明公开
    ANALYTICAL COMPOSITION AND METHOD 有权
    分析组合物和方法

    公开(公告)号：EP1831698A1

    公开(公告)日：2007-09-12

    申请号：EP05804993.3

    申请日：2005-12-23

    申请人： The Robert Gordon University

    发明人： POLLARD, Patricia Mary ,  OFFICER, Simon, Robert Gordon University ,  HUNTER, Catherine, Robert Gordon University ,  PRABHU, G., Radharishna, Robert Gordon University

    IPC分类号： G01N33/58

    CPC分类号： G01N33/587 ,  G01N33/582 ,  G01N2458/40 ,  Y10T436/143333

    摘要： An optically detectable analytical composition comprising a rare earth dopant, a carrier incorporating the rare earth dopant, and at least one of: a chemical linker suitable to bind to a biological binding agent and a biological binding agent is disclosed. Preferred embodiments include a glass micro bead carrier treated to attach a biological binding agent such as a protein, or a nucleic acid. The binding agent can bind to a target species in a sample and thereafter be used to determine the presence, concentration or absence of the target in the sample. The composition can be used in a number of different applications, such as to perform fragment analysis of DNA. The narrow bands emitted by rare earth dopants when excited, allows for a large number of such dopants to be incorporated into an individual carrier, allowing detailed characterisation of the sample to be conducted in a reduced number of sample test runs.

    DIAGNOSTIC NEODYMIUM(III), YTTERBIUM(III), OR ERBIUM(III) ION-LIGAND COMPLEXES
    16.
    发明公开
    DIAGNOSTIC NEODYMIUM(III), YTTERBIUM(III), OR ERBIUM(III) ION-LIGAND COMPLEXES 失效
    ION - 配体络合物ON钕（III），镱（III）或铒（III）在诊断

    公开(公告)号：EP0968424A1

    公开(公告)日：2000-01-05

    申请号：EP98913667.6

    申请日：1998-02-28

    申请人： Akzo Nobel N.V.

    发明人： HOFSTRAAT, Johannes, Willem

    IPC分类号： G01N33/533

    CPC分类号： G01N33/533 ,  G01N2458/40 ,  Y10S435/968 ,  Y10S435/969 ,  Y10S435/971

    摘要： The invention relates to a method for detection of an analyte in a test sample by a specific binding reaction among the analyte, a specific binding partner for the analyte, and an (immuno)reactant provided with a label, characterized in that the label is a lanthanide ion-ligand complex wherein the lanthanide ion is neodymium(III) ion (Nd3+), ytterbium(III) ion (Yb3+), or erbium(III) ion (Er3+) and the ligand comprises or is in contact with a sensitizing moiety which absorbs in the 400-1000 nm region, and preferably in the 400-800 nm region. Further, a diagnostic kit is disclosed as well as a method of detecting an analyte in a matrix of biomedical interest through an oligonucleotide, an antigen, or an antibody attached to a material, preferably core-shell latex or with specific binding sites wherein the antigen or antibody is labeled with the lanthanide ion-ligand complex and brought into contact with the analyte, after which the analyte with the lanthanide-ion complex is immobilized on the material, and, optionally, residual lanthanide-ion complex is removed, after which the sample obtained is irradiated with light in the 400-1000 nm region, and the emitted light from said sample is detected if said analyte is present in the matrix of biomedical interest.

    Homogeneous biospecific assay method using lanthanide chelates as labels
    20.
    发明公开
    Homogeneous biospecific assay method using lanthanide chelates as labels 失效
    同源biospezifische Bestimmungsmethode mit Lanthanidchelaten-Marker。

    公开(公告)号：EP0324323A1

    公开(公告)日：1989-07-19

    申请号：EP88850314.1

    申请日：1988-09-20

    申请人： WALLAC OY

    发明人： Hemmilä, Ilkka ,  Lövgren, Timo

    IPC分类号： G01N33/542 ,  G01N33/533 ,  C12Q1/68

    CPC分类号： G01N33/542 ,  G01N33/582 ,  G01N2458/40 ,  Y10S436/826

    摘要： Method for a homogeneous biospecific affinity assay for determining the content of a substance (analyte) in a biological sample. The assay is carried out in an aqueous reaction medium by means of time-resolved fluorescence spectroscopy and with a biospecific affinity reactant labeled with a lanthanide chelate in which a lanthanide ion exhibiting ionic fluorescence is chelated by a ligand bound covalently to the reactant. The characteristic feature is

    (i) that the lanthanide chelate formed by the lanthanide ion together with the covalently bound ligand forms a fluorescent chelate, and
    (ii) that a modulator is added which stabilizes the lantha­nide chelate so that the lanthanide fluorescence as measured from the medium becomes a practically pure function of the analyte concentration therein.

    摘要翻译： 用于确定生物样品中物质（分析物）含量的均相生物特异性亲和测定法。 通过时间分辨荧光光谱法和用镧系元素螯合物标记的生物特异性亲和反应物在水性反应介质中进行测定，其中显示离子荧光的镧系元素离子被共价结合到反应物上的配体螯合。 其特征是（i）由镧系元素离子与共价结合的配体一起形成的镧系元素螯合物形成荧光螯合物，和（ii）添加调节剂，其使镧系元素螯合物稳定，使得镧系元素荧光从 培养基成为其中分析物浓度的实际纯度函数。 Ø