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    Disarmed T-DNA
    21.
    发明公开
    Disarmed T-DNA 失效
    EntschärfteT-DNS

    公开(公告)号：EP0174166A1

    公开(公告)日：1986-03-12

    申请号：EP85306159.6

    申请日：1985-08-30

    申请人： LUBRIZOL GENETICS INC.

    发明人： Chee, Paula P. ,  Kemp, John D.

    IPC分类号： C12N15/00 ,  C07H21/04 ,  C12P19/34 ,  C12N1/20 ,  C12N5/00

    CPC分类号： C12N15/8205 ,  C12N15/00

    摘要： Disarming mutations of tmr and tms T-DNA genes are disclosed which are useful in an Agrobacterium tumefaciens Ti plasmid-based plant transformation vector. As disarmed T-DNA is compatible with standard plant regeneration protocols, such a vector may be used to introduce a plant-expressible foreign gene into a dicot plant. Methods for use of disarmed T-DNA are provided as are morphologically normal transformed piants. The invention is exemplified by introduction of a Phaseolus vulgaris lectin gene into tobacco.

    摘要翻译： 公开了tmr和tms T-DNA基因的解除突变，其可用于基于根癌土壤杆菌Ti质粒的植物转化载体中。 由于解除武装的T-DNA与标准植物再生方案相容，可以使用这种载体将植物可表达的外源基因导入双子叶植物。 提供解除武装的T-DNA的方法是形态上正常的转化植物。 本发明的例子是通过将菜豆凝集素提取物引入烟草中。

    Nitrogen fixation regulator genes
    22.
    发明公开
    Nitrogen fixation regulator genes 失效
    Regulierende Genefürdie Stickstoffixierung。

    公开(公告)号：EP0164992A2

    公开(公告)日：1985-12-18

    申请号：EP85303925.3

    申请日：1985-06-04

    申请人： LUBRIZOL GENETICS INC.

    发明人： Puhler, Alfred ,  Reilaender, Helmut ,  Weber, Gerhard

    IPC分类号： C12N15/00 ,  C12N1/20

    CPC分类号： C12N15/00

    摘要： The recombinant DNA plasmid comprises a vector, a promoter of a gene of a Rhizobium species, the gene coding for a product normally capable of activating nitrogen fixation, and a foreign structural gene or foreign DNA fragment under control of the promoter. The gene may, for example, be a fixD gene and the foreign structural gene may be a bacterial toxin gene of Bacillus thuringiensis.
    Also disclosed are recombinant DNA plasmids comprising a vector, a promoter of a constitutively expressed gene, and a coding sequence of a gene which codes for a product capable of activating nitrogen fixation.

    摘要翻译： 重组DNA质粒包含载体，根瘤菌基因的启动子，编码通常能够活化固氮的产物的基因，以及在启动子控制下的外源结构基因或外来DNA片段。 该基因可以是例如fixD基因，外来结构基因可以是苏云金芽孢杆菌的细菌毒素基因。 还公开了包含载体，组成型表达基因的启动子和编码能够活化固氮的产物的基因的编码序列的重组DNA质粒。

    Transcription in plants and bacteria
    23.
    发明公开
    Transcription in plants and bacteria 失效
    转录在Pflanzen和Bakterien。

    公开(公告)号：EP0159779A1

    公开(公告)日：1985-10-30

    申请号：EP85301189.8

    申请日：1985-02-22

    申请人： LUBRIZOL GENETICS INC. ,  PURDUE RESEARCH FOUNDATION, INC.

    发明人： Gelvin, Stanton B.

    IPC分类号： C12N15/63 ,  C12N15/70 ,  C12N15/84 ,  C12N1/20

    CPC分类号： C12N15/70 ,  C12N15/8216

    摘要： It is disclosed that a promoter region that drives expression of a 1450 base T R transcript in octopine-type crown gall tumors can also promote expression of a foreign structural gene in bacteria. Use of this dual-purpose promoter region to drive expression of a single copy of a foreign structural gene in both plants and bacteria is taught. The construction of a selectable marker functional in eukaryotes and prokaryotes is exemplified, as are vectors useful in efforts to transform plants.

    摘要翻译： 公开了在章鱼型冠状胆囊肿瘤中驱动1450碱基TR转录物的表达的启动子区域也可促进外源结构基因在细菌中的表达。 教导了这种双用途启动子区域在植物和细菌中驱动外源结构基因单拷贝表达的用途。 在真核生物和原核生物中功能选择性标记的构建被证明是可以用于转化植物的载体。

    Octopine T-DNA promoters
    24.
    发明公开
    Octopine T-DNA promoters 失效
    Octopin T-DNA-Promotoren。

    公开(公告)号：EP0145338A1

    公开(公告)日：1985-06-19

    申请号：EP84307969.0

    申请日：1984-11-16

    申请人： LUBRIZOL GENETICS INC.

    发明人： Barker, Richard F. ,  Kemp, John D.

    IPC分类号： C12N15/82

    CPC分类号： C12N15/8286 ,  C12N15/8216 ,  Y02A40/162

    摘要： The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

    摘要翻译： 公开了在根癌土壤杆菌ATCC 15955中发现的章鱼碱型Ti质粒的T-DNA序列。 发现由真核启动子，核糖体结合位点和多聚腺苷酸化位点限制的十四个开放阅读框。 使用来自pTi15955的启动子和多聚腺苷酸化位点来控制外源结构基因的表达，以实例为例，菜豆常用储存蛋白phase啉，寻常禾本科植物凝集素，苏木精和苏云金芽孢杆菌晶体蛋白的结构基因。 还提供了可用于操纵结构基因和T-DNA序列的载体。

    A T-DNA derived vector
    25.
    发明公开
    A T-DNA derived vector 无效
    冯T-DNS abgeleitete矢量。

    公开(公告)号：EP0140556A1

    公开(公告)日：1985-05-08

    申请号：EP84306233.2

    申请日：1984-09-12

    申请人： LUBRIZOL GENETICS INC.

    发明人： Dahl, Gary A. ,  Sutton, Dennis W. ,  Barker, Richard F.

    IPC分类号： C12N15/04 ,  C12N15/54 ,  C12N5/00

    CPC分类号： C07K14/325 ,  C12N15/8209

    摘要： During the past ten years, the ability to splice DNA from a variety of sources into a recombinant DNA molecule and then to transfer such DNA molecules into different species of prokaryotes and eukaryotes has led to the most exciting revolution in the history of biology. Most of this work has involved the use of bacteria, fungi and animals. Plants have been relatively neglected primarily because suitable vectors were not available. Recently a number of possible vectors have become available but in their natural state they have not been efficient in the transfer and expression of genes from various sources to plant species. The present invention describes some novel discoveries which increase the usefulness of naturally occurring plant DNA vectors in the genetic engineering of plants.

    摘要翻译： 在过去十年中，将DNA从各种来源剪接成重组DNA分子，然后将这些DNA分子转移到不同种类的原核生物和真核生物中的能力已经导致了生物学史上最激动人心的革命。 这项工作大多涉及细菌，真菌和动物的使用。 植物相对被忽略，主要是因为没有合适的载体。 最近，许多可能的载体已经可用，但是在其天然状态下，它们不能有效地将基因从各种来源转移和表达到植物物种。 本发明描述了一些增加植物基因工程中天然存在的植物DNA载体的有用性的新发现。

    A process for the rapid development of hybrid plants and commercial production of hybrid seed
    28.
    发明公开
    A process for the rapid development of hybrid plants and commercial production of hybrid seed 失效
    一种杂交植物的快速发展和在商业基础上生产杂交种子的过程。

    公开(公告)号：EP0044723A2

    公开(公告)日：1982-01-27

    申请号：EP81303287.7

    申请日：1981-07-16

    申请人： LUBRIZOL GENETICS INC.

    发明人： Lawrence, Robert Howard, Jr. ,  Hill, Phillip Eugene

    IPC分类号： A01H1/02

    CPC分类号： A01H1/02

    摘要： Hybrid plants and seeds are produced in increased yields. The process combines the techniques of sexual reproduction to develop new hybrids and seeds, and cloning of original parent plants to provide large scale replication of the original crosses. New hybrid seed varieties can be developed and readied for market in as little as three years, compared to the conventional eight to twelve years normally required for commercial production. Plant breeders are no longer limited to using homozygous parents in the production of new plant hybrids. The process provides a special advantage in the production of increased yields of high purity Brassica seeds. In this instance cloning parents that are maximally self-incompatible results in a parental line consisting of cloned plants that are essentially sib-incompatible; thus, the process eliminates the difficulties of conventional methods which require bud-pollination to maintain sib-incompatible parent lines through inbreeding.

    摘要翻译： 杂交植物和种子中提高的产率制备。 该工艺结合了有性繁殖开发新的混合动力和种子，和原来的亲本植物的克隆提供原十字架的大规模复制的技术。 新的杂交种子品种可以开发，并在短短三年的时间准备好市场，相较于正常所需的商业化生产传统的八到十二年。 植物育种者不再局限于生产新厂房杂交使用纯合子的父母。 该方法提供在生产高纯度的芸苔属种子的产量增加的特殊的优势。 在这种情况下克隆父母确实是最大的自交不亲和的结果对亲代每行有克隆植物是没有本质同胞不相容的; 因此，该方法消除了哪些要求蕾花授粉，以保持通过近亲繁殖同胞不相容亲本系的传统方法的困难。