公开(公告)号：EP3033097A2

公开(公告)日：2016-06-22

申请号：EP14835828.6

申请日：2014-08-14

申请人： Biogen MA Inc. ,  Amunix Operating Inc.

发明人： LIU, Tongyao ,  CHANG, Pei-Yun ,  KULMAN, John ,  SCHELLENBERGER, Volker ,  JIANG, Haiyan ,  PETERS, Robert, T. ,  CHHABRA, Ekta, Seth

IPC分类号： A61K38/36 ,  A61K38/37 ,  A61K35/14 ,  C07K1/00 ,  C07K14/745 ,  C07K14/755

CPC分类号： A61K38/37 ,  A61K9/0019 ,  A61K38/00 ,  C07K14/00 ,  C07K14/755 ,  C07K2319/31

摘要： The present invention provides chimeric proteins comprising a Factor VIII (FVIII) polypeptide and XTEN polypeptides. The FVIII polypeptide can have a reduced affinity for von Willebrand Factor (VWF). XTEN polypeptides of appropriate sizes are inserted into the FVIII polypeptide at particular locations in order to extend the half-life of the FVIII polypeptide. The invention also includes nucleotides, vectors, host cells, and methods of using the chimeric proteins, e.g., to treat bleeding diseases and disorders.

摘要翻译： 本发明提供了包含因子VIII（FVIII）多肽和XTEN多肽的嵌合蛋白质。 FVIII多肽可以对血管性血友病因子（VWF）具有降低的亲和力。 适当大小的XTEN多肽在特定位置插入到FVIII多肽中，以延长FVIII多肽的半衰期。 本发明还包括核苷酸，载体，宿主细胞和使用嵌合蛋白的方法，例如治疗出血疾病和病症。

公开(公告)号：EP2882450A4

公开(公告)日：2016-06-22

申请号：EP13816031

申请日：2013-07-10

申请人： AMUNIX OPERATING INC ,  BIOGEN MA INC

发明人： CHHABRA EKTA SETH ,  LIU TONGYAO ,  CHANG PEI-YUN ,  PETERS ROBERT T ,  KULMAN JOHN

IPC分类号： A61K38/37 ,  A61K38/36 ,  C07K14/755 ,  C12N15/00 ,  C12N15/62 ,  C12P21/08

CPC分类号： C07K14/755 ,  A61K38/36 ,  A61K38/37 ,  A61K47/481 ,  A61K47/55 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/31 ,  C07K2319/35 ,  C07K2319/50 ,  C12N15/11

摘要： The present invention includes a chimeric protein comprising a VWF protein with D′ domain and D3 domain of VWF, one or more XTEN sequence, and a FVIII protein, wherein the VWF fragment, the XTEN sequence, or the FVIII protein are linked to or associated with each other. The chimeric protein can further comprise one or more Ig constant region or a portion thereof (e.g., an Fc region). A polypeptide chain of a VWF fragment is associated with a FVIII polypeptide chain linked to an XTEN sequence. The VWF fragment polypeptide chain can prevent or inhibit binding of endogenous VWF to FVIII protein linked to the XTEN sequence. By preventing or inhibiting binding of endogenous VWF to FVIII protein, VWF fragment can extend half-life of chimeric protein comprising FVIII protein. The invention includes nucleotides, vectors, host cells, use of VWF fragment, or chimeric proteins.

公开(公告)号：EP2755675A4

公开(公告)日：2015-07-08

申请号：EP12831010

申请日：2012-09-12

申请人： AMUNIX OPERATING INC

发明人： SCHELLENBERGER VOLKER ,  SILVERMAN JOSHUA ,  STEMMER WILLEM P ,  WANG CHIA-WEI ,  GEETHING NATHAN ,  SPINK BENJAMIN

IPC分类号： A61K38/26 ,  C07K14/605

CPC分类号： C07K14/605 ,  A61K38/00 ,  C07K2319/31

摘要： The present invention relates to compositions comprising GLP-2 protein or variants thereof linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in the treatment of GLP-2-related conditions.

摘要翻译： 本发明涉及组合物，其包括GLP-2蛋白或其变体连接至延长重组多肽（XTEN），分离的核酸编码的组合物和载体和含同样的宿主细胞，以及在治疗制造和使用搜索组合物的方法 GLP-2相关的条件。

公开(公告)号：EP2882450A2

公开(公告)日：2015-06-17

申请号：EP13816031.2

申请日：2013-07-10

申请人： Amunix Operating Inc. ,  Biogen Idec MA Inc.

发明人： CHHABRA, Ekta, Seth ,  LIU, Tongyao ,  CHANG, Pei-yun ,  PETERS, Robert, T. ,  KULMAN, John

IPC分类号： A61K38/37 ,  C12N15/00 ,  C12P21/08

CPC分类号： C07K14/755 ,  A61K38/36 ,  A61K38/37 ,  A61K47/55 ,  C07K16/00 ,  C07K2319/00 ,  C07K2319/30 ,  C07K2319/31 ,  C07K2319/35 ,  C07K2319/50 ,  C12N15/11

摘要： The present invention includes a chimeric protein comprising a VWF protein with D′ domain and D3 domain of VWF, one or more XTEN sequence, and a FVIII protein, wherein the VWF fragment, the XTEN sequence, or the FVIII protein are linked to or associated with each other. The chimeric protein can further comprise one or more Ig constant region or a portion thereof (e.g., an Fc region). A polypeptide chain of a VWF fragment is associated with a FVIII polypeptide chain linked to an XTEN sequence. The VWF fragment polypeptide chain can prevent or inhibit binding of endogenous VWF to FVIII protein linked to the XTEN sequence. By preventing or inhibiting binding of endogenous VWF to FVIII protein, VWF fragment can extend half-life of chimeric protein comprising FVIII protein. The invention includes nucleotides, vectors, host cells, use of VWF fragment, or chimeric proteins.

摘要翻译： 本发明包括嵌合蛋白，其包含具有D'结构域的VWF蛋白和VWF的D3结构域，一个或多个XTEN序列和FVIII蛋白，其中所述VWF片段，XTEN序列或FVIII蛋白连接到或相关联 与对方 嵌合蛋白可以进一步包含一个或多个Ig恒定区或其部分（例如Fc区）。 VWF片段的多肽链与与XTEN序列连接的FVIII多肽链相关。 VWF片段多肽链可以预防或抑制内源性VWF与与XTEN序列连接的FVIII蛋白的结合。 通过预防或抑制内源VWF与FVIII蛋白的结合，VWF片段可以延长包含FVIII蛋白的嵌合蛋白的半衰期。 本发明包括核苷酸，载体，宿主细胞，VWF片段或嵌合蛋白的用途。

公开(公告)号：EP2814840A1

公开(公告)日：2014-12-24

申请号：EP12868427.1

申请日：2012-07-11

申请人： Amunix Operating Inc. ,  Biogen Idec MA Inc.

发明人： SCHELLENBERGER, Volker ,  CHANG, Pei-yun ,  VARFAJ, Fatbardha ,  DING, Sheng ,  SILVERMAN, Joshua ,  WANG, Chia-wei ,  SPINK, Benjamin ,  STEMMER, Willem, P. ,  GEETHING, Nathan ,  KULMAN, John ,  LIU, Tongyao ,  TOBY, Garabet, G. ,  JIANG, Haiyan ,  PETERS, Robert ,  WANG, Deping ,  MEI, Baisong

IPC分类号： C07K14/755 ,  A61K38/37

摘要： The present invention relates to compositions comprising factor VIII coagulation factors linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of factor VIII-related diseases, disorders, and conditions.

公开(公告)号：EP2755675A2

公开(公告)日：2014-07-23

申请号：EP12831010.9

申请日：2012-09-12

申请人： Amunix Operating Inc.

发明人： SCHELLENBERGER, Volker ,  SILVERMAN, Joshua ,  STEMMER, Willem P. ,  WANG, Chia-Wei ,  GEETHING, Nathan ,  SPINK, Benjamin

IPC分类号： A61K38/26

CPC分类号： C07K14/605 ,  A61K38/00 ,  C07K2319/31

摘要： The present invention relates to compositions comprising GLP-2 protein or variants thereof linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in the treatment of GLP-2-related conditions.

公开(公告)号：EP2470670A1

公开(公告)日：2012-07-04

申请号：EP10814048.4

申请日：2010-08-02

申请人： Amunix Operating Inc.

发明人： SCHELLENBERGER, Volker ,  SILVERMAN, Joshua ,  STEMMER, Willem, Peter ,  WANG, Chia-Wei ,  SPINK, Benjamin ,  GEETHING, Nathan, Carl ,  TO, Wayne

IPC分类号： C12P21/04 ,  A61K38/00

CPC分类号： C12N9/644 ,  A61K38/00 ,  C07K14/001 ,  C07K2319/00 ,  C07K2319/31 ,  C07K2319/50 ,  C12N9/6437 ,  C12N15/70 ,  C12Y304/21022

摘要： The present invention relates to compositions comprising factor IX coagulation factors linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of coagulation factor-related diseases, disorders, and conditions.

公开(公告)号：EP2440228A1

公开(公告)日：2012-04-18

申请号：EP10786730.1

申请日：2010-06-08

申请人： Amunix Operating Inc.

发明人： SCHELLENBERGER, Volker ,  SILVERMAN, Joshua ,  STEMMER, Willem, P. ,  WANG, Chia-wei ,  GEETHING, Nathan ,  CLELAND, Jeffrey. L.

IPC分类号： A61K38/00

CPC分类号： C07K14/605 ,  A23V2200/328 ,  A61K38/00 ,  C07K14/575 ,  C07K14/57563 ,  C07K2319/00 ,  C07K2319/31 ,  C07K2319/50 ,  C12N15/70 ,  C12P21/02 ,  Y10S514/866

摘要： The present invention relates to compositions comprising glucose regulating peptides linked to extended recombinant polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of making and using such compositions in treatment of glucose regulating peptide-related diseases, disorders, and conditions.

公开(公告)号：EP3372617A2

公开(公告)日：2018-09-12

申请号：EP18159324.5

申请日：2011-04-01

申请人： Amunix Operating Inc.

发明人： Schellenberger, Volker ,  Silverman, Joshua ,  Wang, Chia-wei ,  Spink, Benjamin ,  Stemmer, Willem P. ,  Alters, Susan E.

IPC分类号： C07K19/00 ,  C07K16/28

CPC分类号： C07K16/28 ,  C07K2317/622 ,  C07K2317/90 ,  C07K2319/01

摘要： The present invention relates to binding fusion protein compositions comprising targeting moieties linked to extended recombinant polypeptide (XTEN), binding fusion protein-drug conjugate compositions, and XTEN-drug conjugate compositions, isolated nucleic acids encoding the compositions and vectors and host cells containing the same, and methods of using such compositions in treatment of diseases, disorders, and conditions.

公开(公告)号：EP3341009A2

公开(公告)日：2018-07-04

申请号：EP16842718.5

申请日：2016-08-26

申请人： Amunix Operating Inc.

发明人： SCHELLENBERGER, Volker ,  YANG, Fan ,  THAYER, Desiree ,  SIM, Bee-cheng ,  WANG, Chia-wei

IPC分类号： A61K38/16 ,  A61K38/17 ,  A61K38/24

CPC分类号： C07K16/2809 ,  A61K38/00 ,  A61K2039/507 ,  C07K16/30 ,  C07K2317/31 ,  C07K2317/60 ,  C07K2317/622 ,  C07K2319/01

摘要： The present invention relates to bispecific chimeric polypeptide assembly compositions comprising bulking moieties linked to binding domains by cleavable release segments that, when cleaved are capable of concurrently binding effector T cells with targeted tumor or cancer cells and effecting cytolysis of the tumor cells or cancer cells. The invention also provides compositions and methods of making and using the cleavable chimeric polypeptide assembly compositions.