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    EXOSOME RECOVERY METHODS WITH LOW MOLECULAR WEIGHT ORGANIC ZWITTERIONS
    21.
    发明公开
    EXOSOME RECOVERY METHODS WITH LOW MOLECULAR WEIGHT ORGANIC ZWITTERIONS 审中-公开
    EXOSOMGEWINNUNGSVERFAHREN MIT ORGANISCHEN ZWITTERIONEN VON NIEDRIGEM MOLEKULARGEWICHT

    公开(公告)号：EP3060911A4

    公开(公告)日：2017-06-21

    申请号：EP14855837

    申请日：2014-10-15

    申请人： AGENCY SCIENCE TECH & RES

    发明人： GAGNON PETER STANLEY

    IPC分类号： A61K35/16 ,  A61K35/22 ,  G01N33/48

    CPC分类号： G01N33/5076 ,  A61K35/12 ,  B01D15/364 ,  G01N33/5005

    摘要： A method of isolating exosomes includes conducting at least one purification step in the presence of an organic zwitterion having a molecular weight of less than about 350 Daltons, a buffering pK of a negatively charged portion of the organic zwitterion is at least one full pH unit below an operating pH at which the at least one purification step is conducted, and a buffering pK of the positively charged portion of the organic zwitterion is at least one full pH unit above the operating pH.

    摘要翻译： 分离外泌体的方法包括在分子量小于约350道尔顿的有机两性离子存在下进行至少一个纯化步骤，有机两性离子的带负电荷部分的缓冲pK为至少一个低于完全pH单位 进行所述至少一个纯化步骤的操作pH，并且所述有机两性离子的带正电部分的缓冲pK是在所述操作pH以上的至少一个完整pH单位。

    PROCESS FOR SEPARATING A CHARGED SPECIES FROM AN AQUEOUS SYSTEM
    23.
    发明公开
    PROCESS FOR SEPARATING A CHARGED SPECIES FROM AN AQUEOUS SYSTEM 审中-公开
    维多利亚ZUR ABTRENNUNG VON GELADENEN SPEZIES AUD EINEMWÄSSRIGEN系统

    公开(公告)号：EP2188052A1

    公开(公告)日：2010-05-26

    申请号：EP08793866.8

    申请日：2008-08-29

    申请人： Afira IPR B.V.

    发明人： CUSTERS, Johannes Petrus Aldegonda ,  SABLONG, Rafaël Jean ,  LEERMAKERS, Franciscus Adrianus Maria ,  KEURENTJES, Johannes Theodorus Faustinus ,  VAN ASSELDONK, Dirk Theodorus Andreas

    IPC分类号： B01J43/00 ,  B01J49/00 ,  C02F1/42

    CPC分类号： B01J43/00 ,  B01D15/364 ,  B01D15/3876 ,  B01J49/45 ,  C02F1/42

    摘要： The present invention relates to a process for separating a charged species from an aqueous system, wherein the process comprises the following steps: (a) a first aqueous system comprising the charged species is contacted at a first temperature with an ampholytic polymeric 5 system comprising cationic and anionic domains, wherein the charged species is bonded to the ampholytic polymeric system; and (b) the ampholytic polymeric system is contacted with a second aqueous system at a second temperature, wherein the charged species is released to the second aqueous system, wherein the second temperature is higher than the first temperature and wherein the second temperature is higher than the first temperature and wherein the second 10 temperature is less than 60° C.

    摘要翻译： 本发明涉及从含水体系中分离带电物质的方法，其中该方法包括以下步骤：（a）包含带电物质的第一含水体系在第一温度下与包含阳离子的两性聚合物5体系 和阴离子结构域，其中带电物质结合到两性聚合体系; 和（b）所述两性聚合物体系在第二温度下与第二水性体系接触，其中所述带电物质被释放到所述第二水性体系，其中所述第二温度高于所述第一温度，并且其中所述第二温度高于 第一温度，其中第二个10温度低于60℃。

    Mass spectrometric quantitative detection of methyl malonic acid and succinic acid using hilic on a zwitterionic stationary phase
    26.
    发明公开
    Mass spectrometric quantitative detection of methyl malonic acid and succinic acid using hilic on a zwitterionic stationary phase 审中-公开
    甲基丙二酸和丁二酸上的两性离子固定相使用HILIC的质谱定量

    公开(公告)号：EP1962097A1

    公开(公告)日：2008-08-27

    申请号：EP07003771.8

    申请日：2007-02-23

    申请人： SeQuant AB

    发明人： Appelblad, Patrik

    IPC分类号： G01N33/82 ,  B01D15/36

    CPC分类号： G01N30/7233 ,  B01D15/364 ,  G01N30/02 ,  G01N33/50 ,  B01D15/36

    摘要： The invention provides a method for qualitatively and quantitatively detecting methyl malonic acid in a clinical sample that also may contain succinic acid and homocysteine, said method involving a liquid chromatography separation step followed by a mass spectroscopy detection step, said method comprising the steps of:
    a) providing a sample that may contain methyl malonic acid and/or succinic acid and optionally homocysteine;
    b) injecting said sample in a mobile phase comprising a high amount of water-miscible organic solvent;
    c) eluting said mobile phase containing said sample through a liquid chromatography column containing zwitterionic groups covalently bound to carriers as a stationary phase;
    d) detecting the possible presence of methyl malonic acid and succinic acid and optionally homocysteine by mass spectroscopy detection; and
    e) determining the presence and optionally the amounts of said organic molecules using calibration data.

    摘要翻译： 本发明提供了定性和定量检测甲基丙二酸在临床样品中确实使“可包含琥珀酸和高半胱氨酸的方法，所述方法涉及随之而来的是质谱检测步骤液相色谱分离步骤，所述方法包括以下步骤：a ）提供样品确实可以包含甲基丙二酸和/或丁二酸和高半胱氨酸OPTIONALLY; b）以流动相包含水混溶性有机溶剂的量高注入所述样品; c）中洗脱所述移动相通过液相色谱柱包含共价结合到载体作为固定相两性离子基团含有所述样品; d）检测的甲基丙二酸和通过质谱法检测的琥珀酸和高半胱氨酸任选地，可能存在; 和e）确定的采矿的存在和使用的校准数据，所述有机分子的可选地，所述量。