摘要:
A cross-linked flexible sponge adsorbent medium containing substantially uniformly distributed fibrous reinforcement is prepared under such conditions that swelling of the sponge is controlled to a water retention value of 2 to 6; and simultaneously or subsequently, the cross-linked sponge is reacted with a reagent which introduces functional groups into the modified cross-linked sponge. There is also provided apparatus having an inlet for liquid, an outlet for liquid so as to define a path for liquid flow between the inlet and the outlet, an adsorbent medium, obtainable by the above method, being positioned across the liquid flow path in the apparatus.
摘要:
Macromolecular species in a sample are chromatographically separated in a manner analogous to that which would occur in molecular sieve chromatography (gel filtration), by being passed through a separation medium comprised of a non-water-soluble fluid-permeable plug of cross-linked polyacrylamide, where the degree of cross-linking is sufficient to cause discontinuities, gaps or channels in the polymer network large enough to permit water and the macromolecules in the sample to pass through, and yet interfering with the free flow of the macromolecules sufficiently to cause their bulk flow rate through the plug to vary with their molecular weights. By varying the nature and composition of the monomers, the plug can be designed alternatively for a wide variety of types of chromatography, including ion exchange, hydrophobic interaction, affinity, boronate and dye-ligand chromatography.
摘要:
The invention relates to an apparatus useful for the separation of at least one preselected ligate present in a fluid. The invention also features an easily scaled-up membrane affinity separation process which is highly reliable, highly selective, provides a high yield of product, and is further characterized by a high volumetric throughput. The invention utilizes a substantially isotropic porous membrane, to which is associated a preselected ligand, which provides an optimum loading capacity and low dead volume while allowing high filtrate flow rates. Methods for isolating macromolecules having therapeutic value are described.
摘要:
Disclosed are solid supports including an immobilized flocculating agent which can be used as separation and assay media in the field of affinity separations and more particularly, in the field of clinical assays. In a specific embodiment, a flocculating agent such as polyethyleneimine is immobilized within a chromatographic support. The column so formed can then be used as an affinity column for affinity separations or immunoassays.
摘要:
The invention relates to an apparatus useful for the separation of at least one preselected ligate present in a fluid. The invention also features an easily scaled-up membrane affinity separation process which is highly reliable, highly selective, provides a high yield of product, and is further characterized by a high volumetric throughput. The invention utilizes a substantially isotropic porous membrane, to which is associated a preselected ligand, which provides an optimum loading capacity and low dead volume while allowing high filtrate flow rates. Methods for isolating macromolecules having therapeutic value are described.
摘要:
A porous polymeric material has cell sizes within the range 100 to 0.5µm and total pore volume with respect to the overall volume of the material in the range 75 to 98% and includes cross-linked polycondensation polymeric material. The material can be made from a high internal phase emulsion in which the cross-linked polycondensation polymer is formed in the continuous phase. The materials are preferably open interconnected cellular structures. They can be made of a wide range of polycondensation materials. The materials are thus highly porous and light weight and have a range of properties depending primarily on their constituent polycondensation materials.
摘要:
A chromatography stick for separating and collecting two or more ingredients contained in a sample according to chromatography, and a method for the separation and collection. This stick enables to develop a sample uniformly and in parallel and can be repeatedly used by cutting the sample-developed portion, thus being useful for collecting a large quantity of a sample ingredient. The stick is composed of only an adsorbing material such as silica gel and a necessary binder and is formed as a column or plate. In conducting chromatography, a sample is adsorbed at the bottom of the stick, and the bottom is placed on a plate to which a solvent can be freely supplied.