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31.发明授权Method for recovering purified growth hormones from genetically engineered microorganisms 失效从遗传工程微生物中回收纯化生长激素的方法
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32.发明公开Somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations 失效在ALPHA-HELIX 1地区具有改变的SOMATOTROPINS，以及与其他突变体的组合
公开(公告)号：EP0496973A3
公开(公告)日：1993-02-24
申请号：EP91120396.6
申请日：1991-11-28
CPC分类号： C07K1/1133 , C07K14/61
摘要： The present invention relates to somatotropin analogues with amino acid changes in the alpha-helix 1 regions of said somatotropins, alone or in combination with mutations in the alpha-helix 3 and/or alpha-helix 2 regions, plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptide also are provided.
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33.发明公开Somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations 失效生长激素受体激动剂1和Kombinationen mit anderen Mutationen。
公开(公告)号：EP0496973A2
公开(公告)日：1992-08-05
申请号：EP91120396.6
申请日：1991-11-28
CPC分类号： C07K1/1133 , C07K14/61
摘要： The present invention relates to somatotropin analogues with amino acid changes in the alpha-helix 1 regions of said somatotropins, alone or in combination with mutations in the alpha-helix 3 and/or alpha-helix 2 regions, plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptide also are provided.
摘要翻译： 本发明涉及在所述生长激素的α-螺旋1区域中具有氨基酸变化的生长激素类似物，单独或与α-螺旋3和/或α-螺旋2区域中的突变组合，以及与 生长激素的天然氨基酸序列。 所得类似物在持续释放制剂中配制稳定，同时保持生物活性。 此外，还提供了对编码蛋白质和/或多肽的DNA进行定点诱变的方法。
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公开(公告)号：EP0408742A1
公开(公告)日：1991-01-23
申请号：EP90907882.0
申请日：1990-02-07
发明人： EVEN-CHEN, Zeev
CPC分类号： C07K14/005 , C12N15/85 , C12N2730/10122
摘要： On décrit des procédés permettant de produire des particules purifiées d'antigène d'enveloppe de l'hépatite B (HBsAg) dans des cellules de mammifère, qui consistent à faire la culture de cellules de mammifère produisant lesdites particules dans un milieu de culture auquel on a ajouté un sérum exempt de protéines de contamination à poids moléculaire élevé, et à récupérer ensuite les particules purifiées d'antigène d'enveloppe de l'hépatite B. L'extraction par exemple par préfractionnement des molécules ayant un poids moléculaire supérieur à environ 3 x 105 daltons permet de cultiver des cellules dans un milieu de culture contenant des taux élevés de sérum foetal de veau, d'enlever les protéines de contamination à poids moléculaire élevé qui peuvent avoir un effet d'inhibition sur la croissance des cellules, et de simplifier la purification de HBsAg, étant donné que les protéines de contamination à poids moléculaire élevé, qui sont les principaux contaminants, sont extraites par des procédés de purification.
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35.发明公开Growth hormone analogs, pharmaceutical and veterinary compositions containing said analogs and methods for production thereof 失效含有该类似物的生长激素类似物，药物和兽医学组合物及其生产方法
公开(公告)号：EP0319049A3
公开(公告)日：1990-01-03
申请号：EP88121246.8
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
摘要： Disclosed are bGH and hGH analogs having the activity of the respective naturally occurring substances and a similar amino acid sequence varying from the sequence of the respective naturally occurring substances. Further there are disclosed pharmaceutical and veterinary compositions containing said analogs as well as methods for producing and purifying said analogs.
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公开(公告)号：EP0871474A1
公开(公告)日：1998-10-21
申请号：EP95907193.0
申请日：1994-12-29
CPC分类号： C07K1/1136 , A61K38/00 , C07K14/62
摘要： An improved and efficient process for the production of recombinant human insulin by folding of a proinsulin hybrid polypeptide is provided.
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37.发明授权Porcine somatotropins with alterations in the alpha-helix 1 region, and combinations with other mutations 失效在α螺旋1的区域的修改的猪生长激素的，并且与其它突变的组合
公开(公告)号：EP0496973B1
公开(公告)日：1998-02-04
申请号：EP91120396.6
申请日：1991-11-28
CPC分类号： C07K1/1133 , C07K14/61
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38.发明公开Process for the preparation of superoxide dismutase by recombinant DNA-technology 失效Verfahren zur Herstellung von Superoxid dismutase durch rekombinante DNS Technologie
公开(公告)号：EP0691406A1
公开(公告)日：1996-01-10
申请号：EP95114069.8
申请日：1984-07-03
发明人： Aviv, Haim , Gorecki, Marian , Levanon, Avigdor , Oppenheim, Amos , Vogel, Tikva , Zeelon, Elisha , Zeevi, Menachem
CPC分类号： C12N9/0089 , A61K38/00 , C07K14/61 , C12N15/73 , Y10S530/82 , Y10S530/825 , Y10S930/12
摘要： Disclosed is an improved plasmid which upon introduction into a suitable Escherichia coli host cell containing the thermolabile repressor C I , renders the host cell capable, upon increasing the temperature of the host cell to a temerature at which the repressor is destroyed, of effecting expression of a gene encoding superoxide dismutase inserted into the vector, and production of the superoxide dismutase encoded by the gene.
摘要翻译： 公开了一种改进的质粒，其在引入含有不耐热阻遏物CI的合适的大肠杆菌宿主细胞中时，使宿主细胞在宿主细胞的温度升高至阻遏物被破坏的温度时能够发挥作用 将编码超氧化物歧化酶的基因插入载体，并产生由该基因编码的超氧化物歧化酶。
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39.发明公开CLONING AND PRODUCTION OF HUMAN VON WILLEBRAND FACTOR GPIB BINDING DOMAIN POLYPEPTIDES AND METHODS OF USING SAME 失效克隆和生产人类WILLEBRAND因子GPIB结合域多聚体及其使用方法
公开(公告)号：EP0517826A4
公开(公告)日：1993-05-26
申请号：EP91906027
申请日：1991-03-01
发明人： GARFINKEL, LEONARD , RICHTER, TAMAR
IPC分类号： A61K38/43 , A61K38/00 , A61K38/36 , A61P7/02 , A61P9/08 , A61P9/10 , C07K14/00 , C07K14/435 , C07K14/47 , C07K14/745 , C07K14/755 , C12N1/21 , C12N15/09 , C12N15/12 , C12N15/14 , C12P21/00 , C12P21/02 , C12R1/19 , C07K13/00 , A61K37/00 , C12N15/70
CPC分类号： C07K14/755 , A61K38/00
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40.发明公开IMPROVED METHOD FOR PURIFICATION OF RECOMBINANT COPPER/ZINC (CU-ZN) SUPEROXIDE DISMUTASE FROM BACTERIA OR EUCARYOTIC CELLS 失效用于从细菌或真核细胞中纯化重组铜/锌（CU-ZN）超氧化物歧化酶的改进方法
公开(公告)号：EP0499621A4
公开(公告)日：1992-12-09
申请号：EP90917624
申请日：1990-11-07
发明人： BARTFELD, DANIEL , LIFSHITZ, RUTH , HADARY, DANY
IPC分类号： C12N9/02
CPC分类号： C12N9/0089
摘要： The subject invention provides a method for recovering a solution containing purified, enzymatically active Cu-Zn superoxide dismutase or a polypeptide analog thereof having substantially the same amino acid sequence as, and the biological activity of, naturally-occurring Cu-Zn superoxide dismutase from a composition which comprises cells containing Cu-Zn superoxide dismutase or a polypeptide analog thereof. The invention also provides a method of increasing the yield of recovered solutions having an increased concentration of b isoform of an enzymatically-active polypeptide analog of Cu-Zn superoxide dismutase from a composition which comprises cells containing a, b and c isoforms of the polypeptide analog.
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