公开(公告)号：EP3039153B1

公开(公告)日：2018-08-22

申请号：EP14757955.1

申请日：2014-09-01

申请人： Evonik Degussa GmbH

发明人： DISCHERT, Wanda ,  VASSEUR, Perrine ,  FIGGE, Rainer

IPC分类号： C12P13/12 ,  C12N9/10

CPC分类号： C12P13/12 ,  C07K14/24 ,  C07K14/245 ,  C07K14/34 ,  C12N9/1007 ,  C12N15/70 ,  C12N15/77 ,  C12Y201/01013

摘要： The present application is related to a recombinant microorganism optimised for the fermentative production of methionine and/or its derivatives, wherein in said recombinant microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced. The application is also related to a method for optimizing the fermentative production of methionine and/or its derivatives comprising the steps of: c. culturing a recombinant microorganism wherein in said microorganism, the cobalamin-dependent methionine synthase activity and the methionine efflux are enhanced, in an appropriate culture medium comprising a fermentable source of carbon and a source of sulphur, and d. recovering methionine and/or its derivatives from the culture medium.

公开(公告)号：EP3359664A1

公开(公告)日：2018-08-15

申请号：EP16854192.8

申请日：2016-10-05

申请人： Massachusetts Institute Of Technology

发明人： VOIGT, Christopher ,  RYU, Min-Hyung ,  SONG, Mi, Ryoung

IPC分类号： C12N15/03 ,  C12N15/70 ,  C12N15/78 ,  A01N63/00

CPC分类号： C05F11/08 ,  C12N15/52 ,  C12N15/70

摘要： The invention relates to methods for promoting fixed nitrogen from atmospheric nitrogen, and related products. Endophytic bacteria having an exogenous nif cluster promote fixed nitrogen for cereal plants.

公开(公告)号：EP3355939A2

公开(公告)日：2018-08-08

申请号：EP16852709.1

申请日：2016-09-30

申请人： Trustees of Boston University

发明人： CHAN, Tsz Yan Clement ,  COLLINS, James J. ,  LEE, Jeong Wook ,  CAMERON, Douglas Ewen

IPC分类号： A61K48/00 ,  C12N15/11 ,  C12N15/62 ,  C12N15/63 ,  C12N15/85

CPC分类号： C12N15/70 ,  C12N15/635

摘要： Provided herein are systems, methods and compositions for rendering cells or the expression of an effector protein sensitive to a predetermined condition. In one aspect, cells can be rendered dependent upon the presence of an environmental agent, e.g., an exogenous agent, without which the cell will default to expression of a death protein and be killed. In another aspect, cells can be rendered sensitive to the presence of a set of predetermined conditions such that cells will only grow when two or more necessary exogenous agents are supplied, and without either of which, the cells are killed. In this aspect, hybrid transcription factors provide a vast array of possible predetermined conditions.

公开(公告)号：EP2993231B1

公开(公告)日：2018-08-01

申请号：EP15182886.0

申请日：2010-09-23

申请人： UCB Biopharma SPRL

发明人： Ellis, Mark ,  Humphreys, David Paul

IPC分类号： C12N9/50 ,  C12P21/00

CPC分类号： C12N1/20 ,  C07K16/241 ,  C07K16/244 ,  C07K2317/14 ,  C07K2317/20 ,  C07K2317/55 ,  C12N9/52 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19 ,  Y02P20/52

摘要： A recombinant gram-negative bacterial cell comprising one or more of the following mutated protease genes: a. a mutated Tsp gene, wherein the mutated Tsp gene encodes a Tsp protein having reduced protease activity or is a knockout mutated Tsp gene; b. a mutated ptr gene, wherein the mutated ptr gene encodes a Protease III protein having reduced protease activity or is a knockout mutated ptr gene; and c. a mutated DegP gene encoding a DegP protein having chaperone activity and reduced protease activity ; wherein the cell is isogenic to a wild-type bacterial cell except for the mutated Tsp gene and/or mutated ptr gene and/or mutated Deg P gene and optionally a polynucleotide sequence encoding a protein of interest.

公开(公告)号：EP3347483A1

公开(公告)日：2018-07-18

申请号：EP16763821.2

申请日：2016-09-12

申请人： Jennewein Biotechnologie GmbH

发明人： JENNEWEIN, Stefan ,  WARTENBERG, Dirk

IPC分类号： C12P19/18

CPC分类号： C12P19/18 ,  C12N9/1051 ,  C12N15/70 ,  C12Y204/01146

摘要： The present invention relates to methods for the production of oligosaccharides in genetically modified bacterial host cells, as well as to the genetically modified host cells used in the methods. The genetically modified host cell comprises at least one recombinant glycosyltransferase, and at least one nucleic acid sequence coding for a protein enabling the export of the oligosaccharide.

公开(公告)号：EP3091072B1

公开(公告)日：2018-07-04

申请号：EP16169431.0

申请日：2012-12-21

申请人： Caribou Biosciences, Inc.

发明人： BROUNS, Stan Johan Jozef ,  VAN DER OOST, John

IPC分类号： C12N9/22 ,  C12N15/74 ,  C07K14/195

CPC分类号： C12N9/22 ,  A61K38/00 ,  A61K48/005 ,  C07K14/245 ,  C07K14/47 ,  C07K2319/09 ,  C07K2319/22 ,  C07K2319/60 ,  C07K2319/71 ,  C07K2319/80 ,  C07K2319/85 ,  C12N9/16 ,  C12N15/62 ,  C12N15/66 ,  C12N15/70 ,  C12N15/74 ,  C12N15/81 ,  C12N15/82 ,  C12N15/86 ,  C12N15/902 ,  C12N15/907 ,  C12N2310/20 ,  C12Y301/21004

摘要： A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence(Cascade);the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression. A pair of ribonucleotides fused to Fokl dimers may be used to generate double-strand breakages in the DNA to facilitate these applications in a sequence-specific manner.

公开(公告)号：EP3222714A4

公开(公告)日：2018-06-27

申请号：EP14906425

申请日：2014-12-08

申请人： QINGDAO VLAND BIOTECH GROUP CO LTD

发明人： WU XIUXIU ,  WANG HUAMING

IPC分类号： C12N9/16 ,  C12N15/55 ,  C12N15/70

CPC分类号： C12N9/16 ,  C12N15/70

摘要： Provided are mutants PHY1, PHY4 and PHY5 of a wild-type phytase APPA. After being treated for 10min at 80°C , the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 33.85%, 53.11% and 75.86% compared with that of APPA-M; after being treated for 5min at 85°C, the residual enzyme activities of the mutants PHY1, PHY4 and PHY5 are respectively higher by 14.89%, 28.45% and 44.94% compared with that of APPA-M, and the heat resistance of these mutants is significantly higher than that of APPA-M.

公开(公告)号：EP3235831A4

公开(公告)日：2018-06-20

申请号：EP15869319

申请日：2015-12-15

申请人： OXFORD VACMEDIX UK LTD ,  JIANG SHISONG

发明人： JIANG SHISONG ,  XIA XIAOBING ,  DING HANGHAI

IPC分类号： C07K19/00 ,  A61K39/02 ,  A61K39/118 ,  A61K39/12 ,  A61P31/04 ,  A61P31/12

CPC分类号： A61K47/6849 ,  A61K39/02 ,  A61K39/118 ,  A61K39/12 ,  A61K39/39 ,  A61K47/6855 ,  C07K2/00 ,  C07K16/00 ,  C07K19/00 ,  C12N5/0636 ,  C12N15/70 ,  C12N2501/505 ,  C12Q1/686

摘要： Provided are an artificial multi-antigen fusion protein and a preparation method thereof. The fusion protein can effectively stimulate CD8+T and CD4+ T cell immunities, and can be applied to immunodiagnostics or serve as a prophylactic or therapeutic vaccine.

公开(公告)号：EP3225690A4

公开(公告)日：2018-06-20

申请号：EP15862847

申请日：2015-09-01

申请人： INST OF BIOTECHNOLOGY ACADEMY OF MILITARY MEDICAL SCIENCES CHINA

发明人： WU JUN ,  PAN CHAO ,  SUN PENG ,  WANG HENGLIANG ,  LIU BO ,  PENG ZHEHUI ,  ZHU LI ,  CHANG SHAOHONG ,  GONG XIN ,  FENG ERLING ,  WANG BIN ,  ZENG MING

IPC分类号： C12N15/70 ,  A61K39/08 ,  A61K39/104 ,  A61K39/112 ,  A61P31/04 ,  C07K19/00 ,  C12N15/74

CPC分类号： A61K39/08 ,  A61K39/104 ,  C07K19/00 ,  C12N15/70 ,  C12N15/74 ,  Y02A50/472 ,  Y02A50/476 ,  Y02A50/482

摘要： The invention provided a method for preparing a bacterial polysaccharide-modified recombinant fusion protein and use of the bacterial polysaccharide-modified recombinant fusion protein. The method comprises: co-expressing a recombinant fusion protein and the Neisseria meningitidis O-oligosaccharyltransferase PglL in an O-antigen ligase gene-defective bacterium, and linking a polysaccharides endogenous or exogenous for the bacterium to the recombinant fusion protein by the O-oligosaccharyltransferase PglL, to obtain the bacterial polysaccharide-modified recombinant fusion protein. The protein can be used for preparing antibodies against bacterial polysaccharides and vaccines.

公开(公告)号：EP2834360B1

公开(公告)日：2018-05-23

申请号：EP13717574.1

申请日：2013-04-02

申请人： Ajinomoto Co., Inc.

发明人： SYCHEVA, Elena Viktorovna ,  SAMSONOV, Valery Vasilievich ,  SAVRASOVA, Ekaterina Alekseevna ,  EREMINA, Natalia Sergeevna ,  GERASKINA, Natalia Vladimirovna ,  STOYNOVA, Natalia Viktorovna

IPC分类号： C12N15/70

CPC分类号： C12P13/08 ,  C12N15/63 ,  C12N15/70 ,  C12P13/06

摘要： The present invention describes a method for producing a useful metabolite using a bacterium of the family Enterobacteriaceae, particularly a bacterium belonging to the genus Escherichia, which has been modified to contain a gene(s) expression system including elements of the LysR-type protein-regulated transcriptional machinery modified in such a way that auto-inducible positive feedback regulation of the system is mediated by a coinducer. The method is suitable for producing branched-chain L-amino acids, particularly L-valine, L-isoleucine and L-leucine; and D-pantothenic acid.