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419 条记录 (耗时 0.019 秒)

    METHOD FOR PRODUCTION OF BOTULINUM TOXIN
    31.
    发明公开
    METHOD FOR PRODUCTION OF BOTULINUM TOXIN 有权
    用于生产肉毒杆菌毒素

    公开(公告)号：EP2920199A4

    公开(公告)日：2016-10-05

    申请号：EP14832966

    申请日：2014-05-07

    申请人： DAEWOONG CO LTD

    发明人： KIM CHUNG SEI ,  SONG KWAN YOUNG ,  MIN KYOUNG MIN ,  AN YEONG DUK

    IPC分类号： C07K1/36 ,  C07K1/18 ,  C07K1/30

    CPC分类号： C12N9/52 ,  C07K14/33 ,  C12Y304/24069

    摘要： A method of making botulinum toxin comprises treating a culture of a botulinum toxin-producing strain with acid to precipitate a botulinum toxin; adding buffer to the precipitated botulinum toxin, followed by treatment with a protease inhibitor and nuclease, thereby extracting the botulinum toxin; treating the extracted botulinum toxin with acid to precipitate the botulinum toxin and dissolving the precipitate in buffer; and purifying the botulinum toxin by anion exchange chromatography. The use of the method makes it possible to produce a high-purity botulinum toxin by a simple process. The botulinum toxin produced by the method has high purity, and thus has an increased ability to act in a local area. Thus, the systemic circulation of the botulinum toxin is reduced to increase the safety. Accordingly, the botulinum toxin can be used for treatment of neuromuscular disorders, removal of wrinkles, and treatment of spastic hemiplegia and cerebral palsy.

    METHOD FOR PRODUCING -GLUTAMYL-VALYL-GLYCINE CRYSTAL
    33.
    发明公开
    METHOD FOR PRODUCING -GLUTAMYL-VALYL-GLYCINE CRYSTAL 审中-公开
    用于生产谷戊氨酰甘氨酸晶

    公开(公告)号：EP2883960A4

    公开(公告)日：2016-05-18

    申请号：EP13828622

    申请日：2013-08-09

    申请人： AJINOMOTO KK

    发明人： IWASAKI ERIKO

    IPC分类号： C12P21/02 ,  C07K1/30 ,  C07K5/037 ,  C12N15/54

    CPC分类号： C07K5/0819 ,  C07K1/306 ,  C07K5/0215 ,  C12P21/02

    摘要： The present invention provides a method for efficiently producing a ³-glutamyl-valyl-glycine crystal. Specifically, the present invention provides a method for producing a ³-glutamyl-valyl-glycine crystal, which includes the steps of: preparing a mixed solution of valyl-glycine or a salt thereof and ³-glutamyl-valyl-glycine, wherein the mixed solution contains valyl-glycine or the salt thereof in an amount of 20 mass% or more relative to the mass of ³-glutamyl-valyl-glycine; adjusting the amount of valyl-glycine or the salt thereof in the prepared mixed solution to 0.1 mass% or more and less than 20 mass% relative to the mass of ³-glutamyl-valyl-glycine to prepare a ³-glutamyl-valyl-glycine solution; and subjecting the ³-glutamyl-valyl-glycine solution to a crystallization procedure to produce the ³-glutamyl-valyl-glycine crystal.

    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES
    36.
    发明公开
    PROTEIN PURIFICATION IN THE PRESENCE OF NONIONIC ORGANIC POLYMERS AND ELECTROPOSITIVE SURFACES 审中-公开
    蛋白质清洁在非离子有机聚合物和电正完成PRESENCE

    公开(公告)号：EP2961759A1

    公开(公告)日：2016-01-06

    申请号：EP14756237.5

    申请日：2014-02-26

    申请人： Agency for Science, Technology and Research

    发明人： GAGNON, Peter Stanley

    IPC分类号： C07K1/14 ,  B01D15/36 ,  C07K1/30

    CPC分类号： C07K1/165 ,  B01D15/305 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/30 ,  C07K1/303 ,  B01D15/327 ,  B01D15/34

    摘要： A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

    A METHOD OF PURIFYING PROTEINS
    37.
    发明公开
    A METHOD OF PURIFYING PROTEINS 审中-公开
    一种纯化蛋白质的方法

    公开(公告)号：EP2904005A1

    公开(公告)日：2015-08-12

    申请号：EP13777203.4

    申请日：2013-10-01

    申请人： CSL Behring LLC

    发明人： BRINKMAN, Nathan

    IPC分类号： C07K14/79 ,  C07K1/14 ,  C07K1/16 ,  C07K1/30 ,  C07K1/36 ,  C07K14/47

    CPC分类号： C07K14/805 ,  C07K14/4717

    摘要： The present invention relates generally to a method of purifying proteins. More specifically, the present inventions relates to a method of purifying haptoglobin and hemopexin from the same starting material, and uses thereof.

    摘要翻译： 本发明一般涉及纯化蛋白质的方法。 更具体地说，本发明涉及从相同的起始材料中纯化触珠蛋白和血红素结合蛋白的方法及其用途。

    COMPOSITIONS AND METHODS FOR SEPARATING, CHARACTERIZING AND ADMINISTERING SOLUBLE SELENOGLYCOPROTEINS
    40.
    发明公开
    COMPOSITIONS AND METHODS FOR SEPARATING, CHARACTERIZING AND ADMINISTERING SOLUBLE SELENOGLYCOPROTEINS 审中-公开
    组合物和方法分离，表征及管理可溶性SELENOGLYCOPROTEINEN的

    公开(公告)号：EP2686416A4

    公开(公告)日：2014-11-12

    申请号：EP11861852

    申请日：2011-09-16

    申请人： ALLTECH INC

    发明人： KWIATKOWSKI STEFAN ,  POWER RONAN ,  MATNEY CLAYTON ,  GHOROGHCHIAN PAIMAN P ,  OSTERTAG ERIC M

    IPC分类号： C12N1/14 ,  C07K1/30 ,  C07K14/39

    CPC分类号： C07K14/39 ,  A61K9/1273 ,  A61K38/00

    摘要： The invention relates to soluble selenium compositions and methods of production, separation and purification thereof. In particular the present invention provides methods of preparing water soluble selenoglycoproteins (e.g., via extracting selenoglycoproteins from selenium enriched yeast), methods of supplementing a selenium deficient composition via admixing water soluble selenoglycoproteins with the selenium deficient composition, compositions comprising the water soluble selenoglycoproteins and methods of administering the same.

    摘要翻译： 本发明涉及可溶性硒组合物和生产，分离和纯化它们的方法。 特别是在本发明提供了制备水溶性含硒糖蛋白的方法（例如，通过提取从富硒酵母含硒糖蛋白）的补充通过混合水溶性含硒糖蛋白与缺乏硒组合物缺乏硒的组合物的方法，组合物包含水溶性含硒糖蛋白和方法 同样的治理。