摘要:
Provided is a microscope that allows irradiation with uniform illumination light without decreasing the amount of light. Employed is a microscope 1 including an incoherent light source 31 that emits incoherent light I; an optical fiber 35 on which the incoherent light I is incident and which guides the incident incoherent light I by repeated total reflection; a DMD 37 having an array of movable micromirrors each reflecting or transmitting the guided incoherent light I; an objective lens 18 that irradiates a specimen 19 with the incoherent light I reflected or transmitted by the DMD 37 and that collects fluorescence F coming from the specimen 19; a dichroic mirror 17 that splits off the collected fluorescence F coming from the specimen 19 from the incoherent light I; and a CCD camera 13 that is disposed at a position conjugate to the position of the DMD 37 and that detects the fluorescence F coming from the specimen 19 and split off by the dichroic mirror 17.
摘要:
A proposition of the present invention is to provide a laser scanning microscope capable of enhancing the degree of freedom of observation while keeping its structure simple. Accordingly, a laser scanning microscope (100) of the present invention includes a light source part (1), a spectroscopic unit (9) guiding light from the light source part (1) to a specimen plane (16) and guiding the light from the specimen plane (16) to a detector, light path switching units (10 and 13) switching a light path between the spectroscopic unit (9) and the specimen plane (16) to one among a plurality of light paths (R1 and R2) with different routes, and a plurality of light deflecting units (11 and 12) each disposed in each of the plurality of light paths.
摘要:
The invention provides a method for taking a plurality of samples (200) from a substrate (40) with sample material (20) by using an optical microscope (1000) and a stamp (50). At least part of the stamp (50) is transparent and the stamp (50) is arranged in front of the objective (41). The elevations (58) of the stamp (50) are contacted with sample material (20) in a substrate (40).The samples (200) may be replicated to other substrates.
摘要:
A microscope system includes a casing in which an optical element is housed, an airtight specimen chamber, and an environment control unit that controls temperature and humidity in the specimen chamber and also controls temperature inside the casing. An upper plate of the specimen chamber is an open/close lid opened and closed when inserting or removing a biological specimen. The casing becomes sealed by a bottomplate of the specimen chamber as the specimen chamber is set on the casing.
摘要:
A method for sorting particles in a fluid. The method involves generating an optical landscape using an acousto-optic device. Preferably, the optical landscape is arranged to sort particles based on size or refractive index or shape.
摘要:
The invention relates to a device for optical micromanipulation 10, including means 26 for generating a light beam 12 and focusing means for focusing the light beam in a sample 16. According to the invention, the focusing means include an aspherical singlet lens 18 designed to focus the light beam 12 with an angular magnification larger in absolute value than 10. Advantageously, the device for optical micromanipulation 10 is provided with cooperation means for cooperating with an imaging device for imaging the sample such that the combined device comprising the device for optical micromanipulation 10 and the imaging device is capable of exercising the functions of optical micromanipulation and imaging simultaneously. The invention further provides a method for optical micromanipulation, comprising the steps of generating a light beam 12 and focusing the light beam 12 in a sample 16.
摘要:
Beam manipulation member for use in an optical tweezers system, the beam manipulation member comprising at least one optical element, being controllably deformable in order to act on a laser beam in response to signals coming from the optical tweezers system. The beam manipulation member may be used to change the focal distance of the optical tweezers system and also to deflect the laser beam.
摘要:
Apparatus for forming a single or a plurality of threedimensional optical traps, the apparatus comprising: a. A collimated light source that is directed onto an array of focalizing refractive or diffractive elements providing a single or a plurality of focal areas, and b. An array of reflective elements, placed opposite to the said focalizing elements described in a), which reflect back the light into the said focal area. The invention also relates to a method for using this apparatus.