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    Substrate and chip for conducting bioassays
    42.
    发明公开
    Substrate and chip for conducting bioassays 审中-公开
    Substrat und Chip zumDurchführenvon Bioassays

    公开(公告)号：EP2343128A1

    公开(公告)日：2011-07-13

    申请号：EP10183395.2

    申请日：2000-09-17

    申请人： BioArray Solutions Ltd.

    发明人： Seul, Michael ,  Chau, Chiu Wo

    IPC分类号： B01L3/00 ,  G01N33/543

    CPC分类号： B01L3/502792 ,  B01L3/502715 ,  B01L3/50273 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0819 ,  B01L2300/089 ,  B01L2400/0454 ,  C12Q1/6837 ,  G01N15/0266 ,  G01N30/0005 ,  G01N33/54313 ,  G01N2015/0288 ,  G01N2015/149 ,  C12Q2565/607 ,  C12Q2563/155

    摘要： The application is directed to a substantially planar substrate and a chip disposed thereon, which together form part of biological assay system. The substrate further comprises several discrete hydrophilic regions which are part of a planar surface of the substrate. The hydrophilic regions are separated from other hydrophilic regions by a hydrophobic region which is also part of said planar surface. In this way, the hydrophilic regions are designed to accommodate the chip disposed thereon, since the chip has a hydrophilic surface which faces said planar surface when the chip is disposed on the substrate. On the opposing surface of the chip is deposited an array of particles, whereby a biological reagent or binding ligand is bound to the particles.

    摘要翻译： 该应用涉及基本平坦的基底和设置在其上的芯片，它们一起形成生物测定系统的一部分。 衬底还包括几个离散的亲水区域，其是衬底的平面表面的一部分。 亲水区域通过也是所述平面表面的一部分的疏水区域与其它亲水区域分离。 以这种方式，亲水区域被设计成容纳设置在其上的芯片，因为当芯片设置在基板上时，芯片具有面向所述平面表面的亲水表面。 在芯片的相对表面上沉积有颗粒的阵列，由此将生物试剂或结合配体结合到颗粒上。

    IMAGE PROCESSING AND ANALYSIS OF ARRAY DATA
    45.
    发明公开
    IMAGE PROCESSING AND ANALYSIS OF ARRAY DATA 有权
    BILDVERARBEITUNG UND ANALYZE DER MATRIXDATEN

    公开(公告)号：EP1769424A2

    公开(公告)日：2007-04-04

    申请号：EP05764118

    申请日：2005-06-28

    申请人： BIOARRAY SOLUTIONS LTD ,  XIA XIONGWU ,  YIPING GUAN

    发明人： XIONGWU XIA ,  YIPPING GUAN

    IPC分类号： G06T7/00 ,  G06K9/32

    CPC分类号： G06K9/3275 ,  G06K2209/07 ,  G06T7/73 ,  G06T2207/30072

    摘要： Disclosed are methods of processing images, and in particular aligning and orienting grids from a fluorescent array of signal sources, by linking nearest neighbor sources to form a hexagon, and then using the hexagon lines for alignment and orientation of the grid. Also disclosed are methods and algorithms for aligning image with grid and correcting for signal beads which are smaller than a grid field and the shift in a grid field. These methods can be used where the signal sources are fluorescent images from a microarray. Also disclosed are methods of automated watershed clustering following transformation.

    摘要翻译： 公开了处理图像的方法，特别是通过链接最近邻源来形成六边形，并且然后使用六边形线来对准和定向网格，从信号源的荧光阵列对准和定向网格。 还公开了用于将图像与网格对齐并校正小于网格场的信号珠和网格场中的偏移的方法和算法。 这些方法可用于信号源是来自微阵列的荧光图像。 还公开了在转化之后的自动分水岭聚类的方法。

    GENOTYPING OF MULTIPLE LOCI WITH PCR FOR DIFFERENT LOCI AMPLIFICATION AT DIFFERENT TEMPERATURES
    46.
    发明公开
    GENOTYPING OF MULTIPLE LOCI WITH PCR FOR DIFFERENT LOCI AMPLIFICATION AT DIFFERENT TEMPERATURES 审中-公开
    基因分型多个位点的手段，进行PCR扩增不同基因座在不同的温度

    公开(公告)号：EP1756312A1

    公开(公告)日：2007-02-28

    申请号：EP05750553.9

    申请日：2005-05-17

    申请人： Bioarray Solutions Ltd

    发明人： YANG, Jiacheng

    IPC分类号： C12Q1/68 ,  C12P19/34 ,  C07H21/04

    CPC分类号： C12Q1/6881 ,  C07H21/04 ,  C12Q1/686 ,  C12Q2600/16 ,  C12Q2527/15 ,  C12Q2537/143 ,  C12Q2527/101

    摘要： Disclosed is a method of performing simulltaneous PCR amplification of several designated different loci in a sample each including a different target subsequence, using a set of pairs of forward and reverse primers, wherein the pairs are complementary to target subsequences, where different primer pairs are in different reaction chambers and the sample is also present in the reaction chambers, and wherein different primer pairs have different sequences. Different reaction chambers are provided different annealing temperatures, preferably at the same time, such that the annealing temperatures selected enhance annealing conditions for the primer pairs and the target subsequences within the reaction chambers. The method allows PCR to proceed more quickly, which is important to increase throughput in a multiplexed assay, and can be particularly important for HLA-typing in a transplantation setting.

    Light-controlled electrokinetic assembly of particles near surfaces
    48.
    发明公开
    Light-controlled electrokinetic assembly of particles near surfaces 失效
    Lichtgesteuerte elektrokinetische Anordnung von Partikeln in derNähevonOberflächen

    公开(公告)号：EP1726957A2

    公开(公告)日：2006-11-29

    申请号：EP06018875.2

    申请日：1997-04-24

    申请人： BioArray Solutions Ltd.

    发明人： Seul, Michael

    IPC分类号： G01N33/543

    CPC分类号： G01N33/54313 ,  B01J19/0046 ,  B01J2219/00466 ,  B01J2219/00468 ,  B01J2219/00576 ,  B01J2219/00596 ,  B01J2219/00648 ,  B01J2219/00653 ,  B01J2219/00702 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01L3/502715 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/0663 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2300/089 ,  B01L2400/0415 ,  B01L2400/0454 ,  G01N33/6845

    摘要： A method of detecting the formation of an analyte-biomolecule complex comprising the following steps: providing a planar array of biomolecules comprising a plurality of beads having biomolecules attached thereto, wherein the plurality of beads comprises different bead types, said bead types being distinguishable by said biomolecules and each bead type being associated with a unique chemical or physical characteristic that identifies the biomolecules attached to said bead type, wherein beads are arranged in a planar array and biomolecules attached to said beads are exposed to a contiguous liquid phase, said biomolecules capable of forming complexes with corresponding analyte compounds, when said analyte compounds are present in said liquid phase; contacting the biomolecules with a sample that may contain one or more analyte compounds such that, if the analytes are present in the sample, said analytes bind to corresponding biomolecules to form analyte-biomolecule complexes; detecting the formation of the analyte-biomolecule complexes; and identifying the biomolecules of the analyte-biomolecule complexes by correlating the location of the analyte-biomolecule complexes with particular bead types, based on the unique chemical or physical characteristics of the beads.

    摘要翻译： 一种检测分析物 - 生物分子复合物形成的方法，包括以下步骤：提供生物分子的平面阵列，其包含多个附着有生物分子的珠粒，其中所述多个珠粒包含不同的珠粒类型，所述珠粒类型可被所述 生物分子和每种珠粒类型与识别附着于所述珠粒类型的生物分子的独特的化学或物理特征相关联，其中珠粒布置在平面阵列中，并且附着到所述珠粒上的生物分子暴露于连续的液相，所述生物分子能够 当所述分析物质存在于所述液相中时，与相应的分析物质形成络合物; 使生物分子与可能含有一种或多种分析物化合物的样品接触，使得如果分析物存在于样品中，则所述分析物结合相应的生物分子以形成分析物 - 生物分子复合物; 检测分析物 - 生物分子复合物的形成; 并且基于珠的独特化学或物理特性，通过将分析物 - 生物分子复合物与特定珠粒类型的位置相关联来鉴定分析物 - 生物分子复合物的生物分子。

    GEL-SHELL BEADS WITH ADSORBED OR BOUND BIOMOLECULES
    49.
    发明公开
    GEL-SHELL BEADS WITH ADSORBED OR BOUND BIOMOLECULES 审中-公开
    GELHÜLLENKÜGELCHEN以吸附或结合的生物分子

    公开(公告)号：EP1682866A2

    公开(公告)日：2006-07-26

    申请号：EP04817450.2

    申请日：2004-10-26

    申请人： Bioarray Solutions Ltd

    发明人： BANERJEE, Sukanta ,  TAN, Enqing

    IPC分类号： G01N1/00

    CPC分类号： G01N33/5436

    摘要： Disclosed are gel-coated beads (including HydrogelTM -coated beads), which are capable of adsorbing, or absorbing, proteins and other biomolecules onto or into the gel coating. The gel­coated beads with absorbed or adsorbed biomolecules are suitable for use in an assays, purification or other purposes. The beads have a core made from any of a number of materials, including latex, coated with the gel shell. The biomolecules can be retained within the gel, following adsorption, by covalent attachment, or, by selection of conditions of ambient pH and/or ionic strength such that they are retained without further reaction. Therefore, adsorbed proteins would retain the ability to bind to their respective ligands.

    METHOD FOR CONTROLLING SOLUTE LOADING OF POLYMER MICROPARTICLES
    50.
    发明公开
    METHOD FOR CONTROLLING SOLUTE LOADING OF POLYMER MICROPARTICLES 有权
    方法返回与某个溶解的物质的聚合物微粒控制加载

    公开(公告)号：EP1594628A2

    公开(公告)日：2005-11-16

    申请号：EP04703983.9

    申请日：2004-01-21

    申请人： Bioarray Solutions Ltd

    发明人： BANERJEE, Sukanta ,  GEORGESCU, Cecilia ,  SEUL, Michael

    IPC分类号： B05D7/00

    CPC分类号： C08J3/212 ,  C08J3/215 ,  C08J3/22 ,  C08J2325/08

    摘要： Solute-loaded polymer microparticles are obtained by immersing microparticles in a bath comprising a selected solute dissolved in a ternary solvent system. A first solvent of the ternary system is a strong solvent for both the solute and the polymer from which the microparticle was formed. A second solvent is a weak solvent or non-­ solvent for the solute and the polymer (tuning solvent). A third solvent is a weak solvent or non-solvent for the solute and polymer, but serves as a co-solvent with respect to the first and second solvents in that it is miscible with both the first and second solvents. The amount of solute incorporated into the microparticles is controlled by adjusting the ratio of solute with respect to the microparticle polymer, and by adjusting the composition of the ternary solvent system, principally the amount of tuning solvent. The method is particularly useful for providing libraries of combinatorially encoded microparticles containing distinguishable dye loadings, particularly distinguishable fluorescent dye loadings.