Double receptor polynucleotide assay method
    51.
    发明公开
    Double receptor polynucleotide assay method 失效
    Doppel-Rezeptor-Polynukleotid-Bestimmungsverfahren。

    公开(公告)号:EP0453301A2

    公开(公告)日:1991-10-23

    申请号:EP91303516.8

    申请日:1991-04-19

    IPC分类号: C12Q1/68 G01N33/50

    摘要: A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins complexed to the pair of nucleotide sequences.

    摘要翻译: 描述了用于检测多核苷酸靶序列的方法。 该方法涉及形成响应于靶多核苷酸序列而形成的共价或非共价键合的核苷酸序列对,添加各自能够结合一对核苷酸序列中的一个成员的核苷酸序列特异性结合蛋白,并检测特异性结合 与一对核苷酸序列复合的蛋白质。

    METHOD FOR PRODUCING PROTEIN-NUCLEIC ACID COMPLEX, AND METHOD FOR DETECTING TARGET SUBSTANCE
    55.
    发明公开
    METHOD FOR PRODUCING PROTEIN-NUCLEIC ACID COMPLEX, AND METHOD FOR DETECTING TARGET SUBSTANCE 审中-公开
    工艺用于制造检测物质的蛋白质,核酸复合物和方法

    公开(公告)号:EP2865762A1

    公开(公告)日:2015-04-29

    申请号:EP13807729.2

    申请日:2013-06-14

    IPC分类号: C12P19/34 C12Q1/68 G01N33/53

    摘要: Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gin) residue or a lysine (Lys) residue to the 3'-terminal of a nucleic acid using a 3'-terminal addition enzyme that can add a nucleotide to the 3'-terminal of a nucleic acid; and a labeling compound binding step of binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gin) residue using a transglutaminase (TGase) or binding a labeling compound having a glutamine (Gin) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).

    摘要翻译: 提供了一种用于制造核酸探针也可以检测具有良好的灵敏度的目标物质的方法。 一种用于生产核酸样品的方法,包括:加入至少一种核苷三磷酸衍生物具有谷氨酰胺(Gln)残基或赖氨酸(Lys)残基的核酸的3“末端的3'末端添加步骤 使用3'末端加入酶可以做一个核苷酸添加到核酸的3'-末端; 和标记化合物结合的结合的标记化合物具有赖氨酸(Lys)残基和含有标记部分的谷氨酰胺使用转谷氨酰胺酶(TGase)(Gln)残基或结合具有谷氨酰胺(Gln)残基的标记化合物和含有步骤 标记部分使用转谷氨酰胺酶(TGase)的赖氨酸(Lys)残基。