摘要:
A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins complexed to the pair of nucleotide sequences.
摘要:
An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
摘要:
Provided is a method for producing a nucleic acid probe that can detect a target substance with good sensitivity. A method for producing a nucleic acid probe, comprising: a 3'-terminal addition step of adding at least one nucleoside triphosphate derivative having a glutamine (Gin) residue or a lysine (Lys) residue to the 3'-terminal of a nucleic acid using a 3'-terminal addition enzyme that can add a nucleotide to the 3'-terminal of a nucleic acid; and a labeling compound binding step of binding a labeling compound having a lysine (Lys) residue and containing a labeling moiety to the glutamine (Gin) residue using a transglutaminase (TGase) or binding a labeling compound having a glutamine (Gin) residue and containing a labeling moiety to the lysine (Lys) residue using a transglutaminase (TGase).
摘要:
An object of the present invention is to provide a method and means for analyzing biomolecules that can realize, in biomolecule analysis, a wide dynamic range attained by counting the number of biomolecules and rapid analysis. The present invention relates to a method for analyzing biomolecules, comprising the steps of: immobilizing biomolecules 101 to be analyzed on surfaces of magnetic microparticles 108; reacting labeled probe molecules 104 with the biomolecules 101 to be analyzed; collecting and immobilizing the microparticles 108 on a support substrate 110; and measuring a label on the support substrate 110. Since single-molecule immobilized magnetic microparticles are used in the present invention, the number of biomolecules can be counted, and since hybridization and an antigen-antibody reaction are performed with the microparticles having biomolecules immobilized thereon dispersed, the reaction can be rapidly performed. Further, the type and the abundance of biomolecules of interest can be determined at a single molecular level, so as to evaluate, in particular, the absolute concentration of biomolecules.
摘要:
Kits and a method for cleaving double-stranded DNA using Ref and RecA protein and variants thereof at a site having a DNA sequence homologous to the sequence on a single-stranded DNA targeting fragment are disclosed.
摘要:
The present invention concerns compositions and processes that use affinity tags for isolating, and detecting or quantifying analytes, including nucleic acids, proteins and polypeptides. Compositions include nucleic acid compositions and protein compositions with affinity binding pairs, including metal binding peptides and immobilized metals, or peptide affinity groups.
摘要:
A polyelectrolyte having multiple exposed functional groups, each such group being capable of covalently bonding to a molecule, is immobilized on a surface for the purpose of bonding to a biomolecule. The biomolecule can be, for example, a nucleic acid, e.g., an amine functionalized oligonucleotide. The polyelectrolyte can include, e.g., BSA (Bovine Serum Albumin) which is bound to a functionalized surface using a covalent immobilization strategy, e.g., reaction with the surface of a tosyl-activated microparticle. Following such reaction, exposed reactive functional groups on the protein, such as amine, carboxyl, thiol, hydroxyl groups can further be utilized to covalently couple the oligonucleotide of interest using suitable chemistry.