ENZYMES USEFUL FOR PERACID PRODUCTION
    51.
    发明公开

    公开(公告)号:EP2831226A1

    公开(公告)日:2015-02-04

    申请号:EP13712076.2

    申请日:2013-03-13

    IPC分类号: C12N9/18 C11D3/386 C12P7/40

    摘要: Acetyl xylan esterases and variants thereof having perhydrolytic activity are provided for producing peroxycarboxylic acids from carboxylic acid esters and a source of peroxygen. Multi-component peracid generation systems comprising an enzyme catalyst having perhydrolytic activity are also provided, as are methods of using the present enzyme catalyst to produce peroxycarboxylic acids. The polypeptide having perhydrolytic activity may be used to produce peroxycarboxylic acids suitable for use in a variety of applications such as cleaning, disinfecting, sanitizing, bleaching, wood pulp processing, paper pulp processing, and personal care applications.

    Enzymatic synthesis of polyamide in aqueous mini-emulsion
    54.
    发明公开
    Enzymatic synthesis of polyamide in aqueous mini-emulsion 审中-公开
    Enzymatische Polyamidsynthese inwässrigerMiniemulsion

    公开(公告)号:EP2738198A1

    公开(公告)日:2014-06-04

    申请号:EP12008024.7

    申请日:2012-11-29

    摘要: A process for preparing an aqueous polyamide dispersion, said process comprising reacting in an aqueous medium: a) an organic diamine compound (A) and b) an organic dicarboxylic acid compound (B), in the presence of c) at least one hydrolase enzyme (C) which catalyzes a polycondensation reaction of the diamine compound (A) and dicarboxylic acid compound (B) and d) an emulsifier (D), said process being characterized in that at least a fraction of the diamine compound (A) and the dicarboxylic acid compound (B) are provided in the aqueous medium as a disperse phase having an average droplet diameter of from 40 to 500nm.

    摘要翻译: 一种制备水性聚酰胺分散体的方法,所述方法包括在水性介质中反应:a)有机二胺化合物(A)和b)有机二羧酸化合物(B),在c)至少一种水解酶 (C),其催化二胺化合物(A)和二羧酸化合物(B)的缩聚反应和d)乳化剂(D),所述方法的特征在于,至少一部分二胺化合物(A)和 二羧酸化合物(B)作为平均液滴直径为40-500nm的分散相在水性介质中提供。

    Verfahren zur Umsetzung eines Carbonsäureesters unter Verwendung BioH-defizienter Zellen
    56.
    发明公开
    Verfahren zur Umsetzung eines Carbonsäureesters unter Verwendung BioH-defizienter Zellen 审中-公开
    Verfahren zur Umsetzung einesCarbonsäureestersunter Verwendung BioH-defizienter Zellen

    公开(公告)号:EP2730655A1

    公开(公告)日:2014-05-14

    申请号:EP12007663.3

    申请日:2012-11-12

    摘要: Die Erfindung betrifft ein Verfahren zur Umsetzung eines Carbonsäureesters der Formel (I)

            R 1 -A-COOR 2      (I),

    wobei R 1 aus der Gruppe ausgewählt ist, die Wasserstoff, -CH 2 OH, -CHO, -COOR 3 , -CH 2 SH, -CH 2 OR 3 und -CH 2 NH 2 umfasst,
    wobei R 2 aus der Gruppe ausgewählt ist, die Alkyl, bevorzugt Methyl, Ethyl und Propyl, umfasst,
    wobei R 3 aus der Gruppe ausgewählt ist, die Wasserstoff und Alkyl, bevorzugt Methyl, Ethyl und Propyl, umfasst,
    und wobei es sich bei A um einen Alkylrest mit wenigstens vier Kohlenstoffatomen handelt,
    mittels einer Zelle, umfassend den Schritt
    a) Kontaktieren der Zelle mit dem Carbonsäureester in einer wässrigen Lösung,

    wobei es sich bei der Zelle um eine rekombinante Zelle handelt, bei der die Aktivität eines Polypeptides umfassend SEQ ID NO 2 oder eine Variante davon gegenüber dem Wildtyp der Zelle verringert ist, sowie eine dazu geeignete Zelle und deren Verwendungen.

    摘要翻译: 通过电池转化羧酸酯(I)包括在水溶液中使细胞与羧酸酯(I)接触。 细胞是重组细胞。 包含与说明书或其变体中给出的256个氨基酸(SEQ ID NO:2)的肽序列的多肽的细胞活性相比,野生型的细胞减少。 通过电池转化式(R1-A-COOR2)(I)的羧酸酯包括(使细胞与羧酸酯在水溶液中接触,细胞是重组细胞,细胞活性为 包含与说明书或其变体中给出的256个氨基酸(SEQ ID NO:2)的肽序列的多肽相比,野生型的细胞R1:H,-CH 2 OH,-CHO,-COOR 3,-CH 2 SH, -CH 2 OR 3或-CH 2 NH 2; R 2:烷基,优选CH 3,C 2 H 5,C 3 H 8; R 3:H,烷基,优选CH 3,C 2 H 5,C 3 H 8;和A 4 C 4烷基独立 还包括:(1)表达重组烷烃羟化酶的细胞;和(2)包含在水溶液中的细胞和式(R1-A1-COOR2)(II)的羧酸酯的反应混合物。 A1:任选取代的,任选支链和/或环状的4C,优选8C烷基,4C,优选8C芳基或4C,优选8C芳烷基。

    Method for the production of bioproducts
    59.
    发明公开
    Method for the production of bioproducts 有权
    生物制品的生产方法

    公开(公告)号:EP2664674A2

    公开(公告)日:2013-11-20

    申请号:EP13160779.8

    申请日:2009-04-28

    摘要: The present invention relates to an extract of at least one fatty acid and/or oil obtainable by the method comprising (i) maintaining a cell suspension culture of oil-producing plant cells under conditions such that the cultured cells synthesise and secrete at least one fatty acid and/or oil into the cell suspension culture medium; and (ii) extracting the thus secreted at least one fatty acid and/or oil from the cell suspension culture medium. The present invention also relates to a product comprising the extract, such as a food product, cosmetic, or lubricant, and to a biofuel obtainable by the further processing and conversion of the extract of at least one fatty acid and/or oil. The present invention further relates to a cell suspension culture of oil-producing plant cells comprising an inhibitor of fatty acid glyceration, the use of the cell suspension culture to produce at least one fatty acid, and to a plant cell suspension culture medium comprising an inhibitor of fatty acid glyceration. Additionally, the present invention relates to a method for producing biofuels from at least one fatty acid and/or mono-, di- and/or tri-glycerides comprising reacting a first volume of the at least one fatty acid and/or mono-, di- and/or tri-glycerides with a second volume of a reactant selected from an alcohol, alkane or alkene, in the presence of a base catalyst, thereby to form the biofuel, wherein the ratio of first volume to second volume is greater than 1:6, such as at least 1:5, 1:4, 1:3, 1:2, or 1:1.

    摘要翻译: 本发明涉及通过以下方法获得的至少一种脂肪酸和/或油的提取物,所述方法包括(i)在产生培养的细胞合成并分泌至少一种脂肪的条件下保持产油植物细胞的细胞悬浮培养物 酸和/或油注入细胞悬液培养基中; 和(ii)从细胞悬浮培养基中提取如此分泌的至少一种脂肪酸和/或油。 本发明还涉及包含提取物(例如食品,化妆品或润滑剂)的产品,并涉及通过进一步加工和转化至少一种脂肪酸和/或油的提取物可获得的生物燃料。 本发明进一步涉及包含脂肪酸甘油化抑制剂的产油植物细胞的细胞悬浮培养物,使用细胞悬浮培养物产生至少一种脂肪酸,以及涉及包含抑制剂的植物细胞悬浮培养基 的脂肪酸甘油酯。 另外,本发明涉及由至少一种脂肪酸和/或甘油单酯,甘油二酯和/或甘油三酯制备生物燃料的方法,包括使第一体积的至少一种脂肪酸和/或单 - ,二 - 和/ 二 - 和/或三 - 甘油酯与第二体积的选自醇,烷烃或烯烃的反应物在碱催化剂存在下反应,从而形成生物燃料,其中第一体积与第二体积的比率大于 1:6,例如至少1:5,1:4,1:3,1:2或1:1。