摘要:
A method of assembling a droplet actuator assembly (1400) comprises: attaching to a mount structure (1410) a substrate (1434) including an electrode emitting an electric field (1438) affecting a droplet present in the droplet actuator assembly and a magnet assembly (1420) configured to accommodate a magnet (1428) emitting a magnetic field affecting a magnetically responsive bead present in the droplet actuator assembly. At least one of the substrate (1434) and the magnet assembly (1420) are removably attached to the mount structure (1410). Attaching the substrate (1434) may further comprise selecting the substrate from among a plurality of substrates (1434) configured to attach to the mount structure (1410) and including different electric field emission characteristics, and/or selecting the magnet assembly (1420) from among a plurality of magnetic assemblies (1420), each magnet assembly configured to attach to the mount structure (1410) and including different magnet properties.
摘要:
A method, circuit and apparatus for detecting capacitance on a droplet actuator, inter alia, for determining the presence, partial presence or absence of a droplet at an electrode on a droplet actuator by: (a) providing a droplet actuator comprising: (i) a substrate comprising electrodes arranged on the substrate for conducting droplet operations on a surface of the substrate; (ii) a capacitance detection circuit for detecting capacitance at the droplet operations surface at one or more of the electrodes; (b) detecting capacitance at the droplet operations surface at one or more of the electrodes; and (c) determining from the capacitance the presence, partial presence or absence of a droplet at the droplet operations surface at the electrode.
摘要:
A method of preparing a sample for conducting an assay, the method comprising providing an input sample comprising glycoproteins, capturing glycoproteins from the input sample on a solid support, and washing the sample support to remove unbound portions of the input sample.
摘要:
A method, comprising receiving an output voltage signal produced by a power supply superimposing an excitation signal onto the output voltage signal to produce a superimposed signal; connecting the superimposed signal to an electrode in a droplet actuator; suppressing the output voltage signal when detecting an impedance of the electrode; and measuring the impedance of the electrode produced by the excitation signal, wherein the impedance indicates presence of liquid at the electrode.
摘要:
A droplet actuator device comprises magnet sets and electrode sets, wherein: each electrode set comprises one or more electrodes associated with a substrate. Two or more electrode sets are arranged to form an array of electrodes; and configured for conducting one or more droplet operations. Each magnet set comprises one or more magnets. Each magnet set is arranged in a relationship with an electrode set such that magnetic field(s) exerted by the magnet set restrict mobility of magnetically responsive particles to a locus which is proximate to the electrode set. Each magnet set is arranged in relationship to its adjacent magnet set(s), which relationship a polar opposite to the polar orientation of the adjacent magnet set.
摘要:
A droplet actuator device comprises magnet sets and electrode sets, wherein: each electrode set comprises one or more electrodes associated with a substrate. Two or more electrode sets are arranged to form an array of electrodes; and configured for conducting one or more droplet operations. Each magnet set comprises one or more magnets. Each magnet set is arranged in a relationship with an electrode set such that magnetic field(s) exerted by the magnet set restrict mobility of magnetically responsive particles to a locus which is proximate to the electrode set. Each magnet set is arranged in relationship to its adjacent magnet set(s), which relationship a polar opposite to the polar orientation of the adjacent magnet set.