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    Amplification of HTLV-1 sequences from multiple sclerosis patients
    62.
    发明公开
    Amplification of HTLV-1 sequences from multiple sclerosis patients 失效
    多发性硬化症患者HTLV-1序列的扩增

    公开(公告)号：EP0384566A3

    公开(公告)日：1991-11-13

    申请号：EP90300550.2

    申请日：1990-01-18

    申请人： THE WISTAR INSTITUTE

    发明人： Reddy, E. Premkumar ,  Koprowski, Hilary

    IPC分类号： C12Q1/68 ,  C07H21/04

    CPC分类号： C12Q1/6853 ,  C12Q1/6883 ,  C12Q1/702

    摘要： A method for diagnosing multiple sclerosis (MS) involves ampli­fication of DNA sequences present in peripheral blood mononuclear cells of MS patients. The amplification is accomplished by use of the polymerase chain reaction employing primers derived from the sequence of HTLV-I, the aetiologic agent of adult T-cell leukemia. Particularly suitable primers are derived from the gag and env regions of the proviral genome.

    摘要翻译： 用于诊断多发性硬化（MS）的方法涉及扩增存在于MS患者的外周血单核细胞中的DNA序列。 使用引物来源于HTLV-I（成人T细胞白血病的病原体）序列的聚合酶链反应完成扩增。 特别合适的引物来自前病毒基因组的gag和env区。

    Methods for detecting growth factor receptor expression
    68.
    发明公开
    Methods for detecting growth factor receptor expression 失效
    Verfahren zum Nachweis von Wachstumsfaktorrezeptorexpression。

    公开(公告)号：EP0339884A1

    公开(公告)日：1989-11-02

    申请号：EP89303998.2

    申请日：1989-04-21

    申请人： THE WISTAR INSTITUTE OF ANATOMY AND BIOLOGY

    发明人： Rakowicz-Szulczynska, Ewa

    IPC分类号： G01N33/566 ,  G01N33/567 ,  G01N33/68 ,  G01N33/574

    CPC分类号： G01N33/74 ,  Y10S436/813 ,  Y10S436/824

    摘要： Growth factor receptor expression is detected by formation of a precipitate of polysome-bound receptor and growth factor on room-temperature incubation of mem­brane-free cytoplasm from a tumor cell with excess growth factor if the tumor cell contains actively synthesizing receptor capable of binding to the growth factor. Alter­natively isolated nuclei of a tumor cell are incubated at room temperature with varying concentrations of labelled growth factor in pure membrane-free cytoplasm of the tumor cell, in cytoplasm of the tumor cell con­taining cell membrane, in synthetic medium and in syn­thetic medium containing microsomal fractions from the tumor cell, so as to cause the nuclei to incorporate the labelled growth factor, and the levels of nuclear uptake in each fraction are compared.

    摘要翻译： 如果肿瘤细胞含有主动合成的能够结合到受体的受体的受体，则通过在具有过量生长因子的肿瘤细胞的无膜细胞质的室温培养中形成多聚糖结合受体和生长因子的沉淀来检测生长因子受体表达 生长因子 或者，将分离的肿瘤细胞核在室温下与不同浓度的标记生长因子在肿瘤细胞的纯无膜细胞质，含有细胞膜的肿瘤细胞的细胞质，合成培养基和含有微粒体分数的​​合成培养基中孵育 从肿瘤细胞中引起细胞核结合标记的生长因子，并比较每个部分的核摄取水平。