公开(公告)号：EP0689592A1

公开(公告)日：1996-01-03

申请号：EP94908704.0

申请日：1994-02-02

申请人： Xoma Corporation

发明人： THEOFAN, Georgia ,  HORWITZ, Arnold ,  BURKE, David ,  BALTAIAN, Manik ,  GRINNA, Lynn

IPC分类号： C12N15 ,  A61K38 ,  A61P7 ,  A61P9 ,  A61P11 ,  A61P13 ,  A61P31 ,  A61P39 ,  C07H21 ,  C07K14 ,  C07K16 ,  C07K19 ,  C12N1 ,  C12N5 ,  C12P21 ,  C12R1

CPC分类号： C07K19/00 ,  A61K38/00 ,  C07K14/4742 ,  C07K2319/00 ,  C12N15/85 ,  C12N2830/00 ,  C12N2830/15 ,  C12N2830/30 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/44

摘要： Disclosed are novel bactericidal/permeability-increasing (BPI) protein products wherein cysteine residue number 132 or 135 is replaced by another amino acid residue, preferably an alanine or serine residue and/or wherein the leucine residue at position 193 is the carboxy terminal residue. Also disclosed are DNA sequences encoding methods for the production of the same in appropriate host cells, and stable homogeneous pharmaceutical compositions containing the analogs suitable for use treatment of gram negative bacterial infection and its sequelae.

公开(公告)号：EP0659216A1

公开(公告)日：1995-06-28

申请号：EP94921008.0

申请日：1994-06-30

申请人： Cohen-Haguenauer, Odile

发明人： Cohen-Haguenauer, Odile

IPC分类号： C12N15 ,  A61K31 ,  A61K48 ,  C07H21 ,  C12N5

CPC分类号： C12N15/86 ,  C12N2740/13043 ,  C12N2830/32 ,  C12N2830/85 ,  C12N2840/203 ,  C12N2840/206 ,  C12N2840/44

摘要： The invention relates to a recombinant vector for the cloning and/or expression and/or transfer of an exogenous nucleotide sequence, said vector being characterized in that it comprises any sequence contained in the fragment C-laI-PvuII comprising the nucleotides 7702 and 1527 of the sequence given in figure (1) and comprising the chain LTR comprised between the nucleotides 7842 and 144, the site PBS starting at the nucleotide 145, the encapsidation sequence comprised in the chain of 250 nucleotides following the end of the LTR sequence, said sequence being capable of controlling the cloning and/or expression and/or tranfer of the exogenous sequence, regardless of its transcriptional orientation with respect to the transcriptional orientation of the virus. The invention also relates to the utilisation of said vector for the transfer and/or cloning and/or expression of genes particularly for a genic therapy.

公开(公告)号：EP0658198A1

公开(公告)日：1995-06-21

申请号：EP93919176.0

申请日：1993-08-26

申请人： Beiersdorf Aktiengesellschaft ,  Gesellschaft für Biotechnologische Forschung mbH (GBF)

发明人： DIRKS, Wilhelm ,  WIRTH, Manfred ,  HAUSER, Hansjörg ,  EICHNER, Wolfram ,  ACHTERBERG, Volker ,  DÖRSCHNER, Albrecht ,  MEYER-INGOLD, Wolfgang ,  MIELKE, Heiko

IPC分类号： C12N15 ,  A61K38 ,  A61P43 ,  B29C47 ,  C07H21 ,  C07K14 ,  C12N5 ,  C12P21 ,  C12Q1

CPC分类号： C07K14/49 ,  A61K38/00 ,  B29C47/0019 ,  B29C47/76 ,  C12N15/67 ,  C12N15/85 ,  C12N2840/203 ,  C12N2840/44

摘要： Multicistronic expression units allow the equimolar expression of the genes located in the corresponding cistrons. These expression units are particularly suitable for the recombinant production of proteins composed of two or more polypeptide subunits.

公开(公告)号：EP0546073A1

公开(公告)日：1993-06-16

申请号：EP91916470.0

申请日：1991-08-28

申请人： GenPharm International, Inc.

发明人： LONBERG, Nils ,  KAY, Robert, M.

IPC分类号： A01K67 ,  A61K39 ,  C07K14 ,  C07K16 ,  C12N5 ,  C12N15 ,  C12P21 ,  A61K38

CPC分类号： C12N15/907 ,  A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/05 ,  A01K2227/105 ,  A01K2267/01 ,  A61K38/00 ,  C07K14/7051 ,  C07K16/00 ,  C07K16/2812 ,  C07K16/3007 ,  C07K16/4266 ,  C07K16/4291 ,  C07K16/44 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/56 ,  C12N5/163 ,  C12N15/00 ,  C12N15/85 ,  C12N15/8509 ,  C12N15/87 ,  C12N15/90 ,  C12N2830/008 ,  C12N2830/15 ,  C12N2830/36 ,  C12N2830/60 ,  C12N2840/44

摘要： L'invention se rapporte à des animaux non humains transgéniques, qui sont capables de produire des anticorps hétérologues, c'est-à-dire des anticorps pour lesquels codent des gènes à chaînes lourdes et légères d'immunoglobuline qu'on ne trouve normalement pas dans le génome de cette espèce d'animal non humain. Dans un aspect de la présente invention, des transgènes codant pour des chaînes lourdes et légères d'immunoglobuline humaine hétérologue non remaniée sont introduits dans un animal non humain, de façon à obtenir un animal transgénique capable de produire des anticorps pour lesquels codent des gènes d'immunoglobuline humains. Ces anticorps hétérologues humains sont produits dans des lymphocytes B qu'on immortalise ensuite, par exemple en les faisant fusionner avec une lignée cellulaire d'immortalisation, telle qu'un myélome, ou en manipulant ces lymphocytes B au moyen d'autres techniques en vue de perpétuer une lignée cellulaire capable de produire un anticorps hétérologue monoclonal. La présente invention se rapporte également à des transgènes d'immunoglobuline à chaînes lourdes et légères permettant de produire des animaux non humains transgéniques, ainsi qu'à des procédés et à des vecteurs permettant de dissocier les locus d'immunoglobuline endogène dans l'animal transgénique. L'invention se rapporte en outre à des procédés permettant de générer un répertoire de segments de gènes de la région variable de l'immunoglobuline synthétique, destiné à être utilisé dans la construction de transgènes, ainsi qu'à des procédés permettant d'induire une production d'anticorps hétérologues grâce à l'utilisation d'animaux contenant des transgènes d'immunoglobuline à chaînes lourdes et légères remaniée ou non remaniée hétérologue.

公开(公告)号：EP0411678B1

公开(公告)日：1992-01-08

申请号：EP90118215.4

申请日：1985-12-03

申请人： GENETICS INSTITUTE, INC.

发明人： Fritsch, Edward ,  Jacobs, Kenneth ,  Hewick, Rodney M.

IPC分类号： C12N15/16 ,  C12P21/02 ,  C12N5/10 ,  C07K13/00

CPC分类号： C07K14/505 ,  C12N15/85 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/002 ,  C12N2830/42 ,  C12N2830/60 ,  C12N2830/85 ,  C12N2840/44

公开(公告)号：EP0401362A1

公开(公告)日：1990-12-12

申请号：EP90901476.0

申请日：1989-12-22

申请人： GENZYME CORPORATION

发明人： RASMUSSEN, James ,  BARSOMIAN, Gary ,  BERGH, Michel

IPC分类号： A61F9 ,  C12N9 ,  C12N15 ,  A61B18 ,  A61M1

CPC分类号： C12N15/86 ,  A61B2018/206 ,  A61F9/00736 ,  A61F9/008 ,  A61F9/00802 ,  A61F2009/0087 ,  A61F2009/00887 ,  C12N9/2402 ,  C12N15/85 ,  C12N2710/14143 ,  C12N2830/00 ,  C12N2830/60 ,  C12N2840/20 ,  C12N2840/44 ,  C12Y302/01045

摘要： L'invention concerne une cellule eucaryotique produisant de la glucosyl-céramidase de recombinaison active de manière enzymatique. Elle concerne également une cellule comprenant de l'acide nucléique codant la glucosyl-céramidase active de manière enzymatique, ainsi qu'un organisme eucaryotique contenant ladite cellule. Elle concerne en outre un procédé de production de glucosyl-céramidase active de manière enzymatique, comprenant les étapes consistant à introduire de l'acide nucléique codant la glucosyl-céramidase dans une cellule eucaryotique, ce qui induit la cellule à exprimer de la glucosyl-céramidase, et à purifier la glucosyl de la cellule.

公开(公告)号：EP0351585A3

公开(公告)日：1990-05-09

申请号：EP89111481.1

申请日：1989-06-23

申请人： Juridical Foundation The Chemo-Sero-Therapeutic Research Institute

发明人： Miyazaki, Junichi Higashimachi ,  Yamamura, Ken-ichi Higashimachi ,  Araki, Masatake ,  Yonemura, Hiroshi ,  Nozaki, Chikateru

IPC分类号： C12N15/85 ,  C12N5/06 ,  C12P21/02

CPC分类号： C07K14/005 ,  C12N9/2471 ,  C12N15/85 ,  C12N2730/10122 ,  C12N2800/108 ,  C12N2830/00 ,  C12N2830/005 ,  C12N2830/15 ,  C12N2830/60 ,  C12N2830/90 ,  C12N2840/44 ,  C12Y302/01023

摘要： An expression vector for the expression of a desired gene in an animal cell is described, which contains a chicken β-­actin gene promoter or a derivative thereof and downstream thereof a restriction enzyme site for the incorporation of a desired gene. Furthermore corresponding recombinant expres­sion vectors, transformed microorganisms and processes for the expression of a desired gene in a host cell are des­cribed.

公开(公告)号：EP0337359A3

公开(公告)日：1990-02-07

申请号：EP89106339.8

申请日：1985-07-04

申请人： SANDOZ AG ,  SANDOZ-PATENT-GMBH ,  SANDOZ-ERFINDUNGEN Verwaltungsgesellschaft m.b.H.

发明人： Clark, Steven C. ,  Kaufman, Randal J. ,  Wong, Gordon G. ,  Wang, Elisabeth A.

IPC分类号： C12N15/00 ,  C12N5/00 ,  C07H21/04 ,  C12N1/20 ,  C12N1/16 ,  C12P21/02 ,  A61K37/02 ,  C07K3/00

CPC分类号： C12N15/85 ,  A61K38/00 ,  C07K14/535 ,  C12N15/70 ,  C12N15/81 ,  C12N2840/44

摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises:
preparing RNA from a cell that produces CSF;
preparing polyadenylated messenger RNA from said RNA;
preparing single stranded cDNA from said messenger RNA;
converting the single stranded cDNA to double stranded cDNA;
inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;
picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;
transfecting the plasmid DNA into suitable host cells for expressing CSF protein;
culturing the transfected cells and assaying the supernatant for CSF activity; and
selecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity. Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line. The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.

公开(公告)号：EP0337359A2

公开(公告)日：1989-10-18

申请号：EP89106339.8

申请日：1985-07-04

申请人： SANDOZ AG ,  SANDOZ-PATENT-GMBH ,  SANDOZ-ERFINDUNGEN Verwaltungsgesellschaft m.b.H.

发明人： Clark, Steven C. ,  Kaufman, Randal J. ,  Wong, Gordon G. ,  Wang, Elisabeth A.

IPC分类号： C12N15/00 ,  C12N5/00 ,  C07H21/04 ,  C12N1/20 ,  C12N1/16 ,  C12P21/02 ,  A61K37/02 ,  C07K3/00

CPC分类号： C12N15/85 ,  A61K38/00 ,  C07K14/535 ,  C12N15/70 ,  C12N15/81 ,  C12N2840/44

摘要： A method for preparing and isolating a transformation vector containing CSF/cDNA is described. The method comprises:
preparing RNA from a cell that produces CSF;
preparing polyadenylated messenger RNA from said RNA;
preparing single stranded cDNA from said messenger RNA;
converting the single stranded cDNA to double stranded cDNA;
inserting the double stranded cDNA into transformation vectors and transforming bacteria with said vector to form colonies;
picking pools of 200 to 500 colonies each and isolating plasmid DNA from each pool;
transfecting the plasmid DNA into suitable host cells for expressing CSF protein;
culturing the transfected cells and assaying the supernatant for CSF activity; and
selecting CSF positive pools and screening the colonies used to make the pool to identify a colony having CSF activity.
Also described are a cDNA coding for a protein having CSF activity (i.e. CSF/cDNA), a microorganism or cell line transformed with a recombinant vector containing such CSF/cDNA, and a method for producing CSF protein by expressing said CSF/cDNA by culturing a microorganism or cell line.
The invention also provides a method of purifying the CSF proteins and the purified proteins so produced.

摘要翻译： 描述了制备和分离含有CSF / cDNA的转化载体的方法。 该方法包括：从产生CSF的细胞制备RNA; 从所述RNA制备聚腺苷酸化的信使RNA; 从所述信使RNA制备单链cDNA; 将单链cDNA转化为双链cDNA; 将双链cDNA插入转化载体并用所述载体转化细菌以形成菌落; 分别取200至500个菌落的池，每个池中分离出质粒DNA; 将质粒DNA转染到合适的宿主细胞中以表达CSF蛋白; 培养转染的细胞并测定上清液的CSF活性; 并选择CSF阳性池并筛选用于制备池的菌落以鉴定具有CSF活性的菌落。 还描述了编码具有CSF活性的蛋白质（即CSF / cDNA）的cDNA，用含有这种CSF / cDNA的重组载体转化的微生物或细胞系，以及通过培养表达所述CSF / cDNA来产生CSF蛋白的方法 微生物或细胞系。 本发明还提供了一种纯化CSF蛋白质和如此制备的纯化蛋白质的方法。

公开(公告)号：EP0278776A2

公开(公告)日：1988-08-17

申请号：EP88301190.0

申请日：1988-02-12

申请人： GENENTECH, INC.

发明人： Lawn, Richard Mark ,  Wion, Karen Lynne ,  Vehar, Gorden Allen

IPC分类号： C12N15/12 ,  C07K14/745 ,  C12N5/10 ,  C12N1/21

CPC分类号： C12N15/85 ,  A61K38/00 ,  C07K14/005 ,  C07K14/475 ,  C07K14/745 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/32 ,  C07K2319/50 ,  C12N9/6429 ,  C12N2710/16622 ,  C12N2800/108 ,  C12N2830/42 ,  C12N2840/44 ,  C12Y304/21005

摘要： DNA isolates coding for tissue factor protein and derivatives thereof and methods of obtaining such DNA and producing tissue factor protein and derivatives thereof using recombinant expression systems for use in therapeutic compositions for the treatment of coagulation disorders.

摘要翻译： 编码组织因子蛋白质的DNA分离物及其衍生物以及使用用于治疗凝血障碍的治疗组合物的重组表达系统获得此类DNA并产生组织因子蛋白及其衍生物的方法。