公开(公告)号：EP2826564B1

公开(公告)日：2018-12-26

申请号：EP14182497.9

申请日：2010-11-22

Applicant: 3M Innovative Properties Company

Inventor： Halverson, Kurt J. ,  Kenney, Raymond J. ,  Benson, Olester, Jr. ,  Johnston, Raymond P. ,  Mao, Guoping ,  Fleming, Patrick R. ,  Tiers, George Van Dyke ,  Jing, Naiyong

IPC: B01L3/00 ,  B29C59/00 ,  G01N21/64

CPC classification number: G01N21/75 ,  B01L3/50853 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/044 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0851 ,  B01L2300/0887 ,  B29C35/0888 ,  B29C59/046 ,  B29C59/08 ,  B29C59/10 ,  B29C59/14 ,  B29C59/16 ,  B29C2035/0827 ,  B29C2059/023 ,  G01N21/03 ,  G01N21/253 ,  G01N21/31 ,  G01N21/55 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/0346 ,  G01N2201/0446 ,  G01N2201/064 ,  G01N2201/08

Abstract: The disclosure provides microstructured articles and methods useful for detecting an analyte in a sample. The articles include microwell arrays. The articles can be used with an optical system component in methods to detect or characterize an analyte.

公开(公告)号：EP3322972A1

公开(公告)日：2018-05-23

申请号：EP16776229.3

申请日：2016-04-08

Applicant: Bactusense Technologies Ltd.

Inventor： DU-NOUR, Ofer

IPC: G01N21/47

CPC classification number: G01N21/4788 ,  A61B5/7257 ,  G01D5/266 ,  G01N21/4738 ,  G01N2021/4726 ,  G01N2021/4797 ,  G01N2201/0446 ,  G02B21/06 ,  G02B21/362

Abstract: Systems for the monitoring of bacterial levels in samples, using spectral analysis of the light diffracted from a substrate with an ordered array of pores having diameters enabling the targets to enter them. The trapping pore array is cyclically illuminated by light of different wavelengths, and the light diffracted from the pore array is imaged by a 2-dimensional detector array, with one pixel, or a small group of pixels receiving light from each associated pore. The temporal sequence of frames provides a series of images, each from the reflection of a different wavelength. A time sequenced readout of the signal from the pixel or pixels associated with each pore region, provides a spectral plot of the reflected light from that pore region. Spectral analysis of the light intensity from this series of different wavelength enables the effective optical thickness (EOT) of each pore to be extracted.

公开(公告)号：EP3250908A1

公开(公告)日：2017-12-06

申请号：EP16744003.1

申请日：2016-01-26

Applicant: California Institute of Technology

Inventor： KIM, Jinho ,  YANG, Changhuei

IPC: G01N21/62 ,  C12M1/34

CPC classification number: G02B21/365 ,  G01N21/6452 ,  G01N21/6456 ,  G01N21/6458 ,  G01N2021/6478 ,  G01N2201/0446 ,  G02B21/0076 ,  G02B21/16 ,  H04N5/2258

Abstract: An imaging device for bright field Fourier ptychographic imaging and fluorescence imaging comprises a first fluorescence illumination source configured to provide excitation light of a first range of wavelengths to a transparent well; an optical system having a pair of objectives positioned oppositely; and an image sensor configured to receive light propagated through the optical system from the transparent well. The image sensor is configured to acquire a sequence of uniquely illuminated intensity measurements of light passing through a sample in the well based on sequential illumination at different illumination angles from a variable illumination source, and further configured to acquire a first fluorescence image of the sample based on light emitted by the sample in response to excitation light of the first range of wavelengths.

公开(公告)号：EP2980560A4

公开(公告)日：2016-11-16

申请号：EP14774367

申请日：2014-02-19

Applicant: SONY CORP

Inventor： MORIMOTO NAOKI ,  KAI SHINICHI

IPC: G01N21/01 ,  G01N21/17 ,  G01N21/27 ,  G01N21/64

CPC classification number: G01N21/6428 ,  G01N21/01 ,  G01N21/27 ,  G01N21/274 ,  G01N21/6452 ,  G01N2021/0175 ,  G01N2021/1753 ,  G01N2021/178 ,  G01N2021/1789 ,  G01N2021/6419 ,  G01N2021/6439 ,  G01N2021/6441 ,  G01N2201/0446 ,  G01N2201/0453 ,  G01N2201/0484

Abstract: There is provided a data processing apparatus including: a data determination portion that specifies, in each of first and second light intensity distribution data, an analysis range corresponding to a storage area for storing a detection target, the first and second light intensity distribution data being acquired on the basis of light emitted from first and light sources to a detection area; and a mode selection portion that selects an operation mode of the data determination portion. The mode selection portion selects one of a first mode in which the data determination portion specifies the analysis range in each of the first light intensity distribution data and the second light intensity distribution data, and a second mode in which the data determination portion specifies the analysis range in the second light intensity distribution data on the basis of information on the analysis range of the first light intensity distribution data.

公开(公告)号：EP2927671A4

公开(公告)日：2015-11-11

申请号：EP13859389

申请日：2013-10-29

Applicant: PANASONIC IP MAN CO LTD

Inventor： NAGATOMI KENJI ,  NAKATANI MORIO ,  NAKATANI MASAYA ,  OKI AKIO

IPC: G01N21/64 ,  B01L3/00 ,  G01N21/07

CPC classification number: G01N21/6486 ,  B01L3/502761 ,  B01L3/5085 ,  B01L2200/0668 ,  B01L2300/0645 ,  B01L2300/0806 ,  B01L2300/0864 ,  B01L2300/0893 ,  B01L2300/168 ,  B01L2400/0415 ,  G01N21/03 ,  G01N21/07 ,  G01N21/6452 ,  G01N21/69 ,  G01N2021/0346 ,  G01N2201/0446 ,  G01N2201/0612

Abstract: [Summary] There is provided a sample-holding carrier that can efficiently irradiate a sample in a well with light and a fluorescence detection device using the same. [Object] A biosensor substrate (1) includes: a base substrate (11) to which excitation light is entered from an under face; a reflective film (16) disposed on the top face side of the base substrate (11) and having electrical conductivity; a plurality of wells (13) disposed on the top face side of the reflective film (16) and having a bottom face portion (13a); and a conductive ring (15) that applies a voltage to the reflective film (16) from the outside. When a sample prepared by applying fluorescence labeling to red blood cells is accommodated in the wells (13), the red blood cells are uniformly arranged on the bottom face portion (13a) by applying a positive voltage to the reflective film (16) because the red blood cells are generally negatively charged. Thus, it is possible to efficiently irradiate individual red blood cells with excitation light.

公开(公告)号：EP2778231A4

公开(公告)日：2015-08-12

申请号：EP12847822

申请日：2012-10-23

Applicant: HAMAMATSU PHOTONICS KK

Inventor： SUGIYAMA NORIKAZU ,  KATAOKA TAKUJI ,  ARATA IKUO

IPC: C12Q1/02 ,  C12M1/34 ,  C12N5/071 ,  G01N21/49 ,  G02B21/06

CPC classification number: G01N21/47 ,  G01N21/49 ,  G01N21/59 ,  G01N33/4833 ,  G01N33/5005 ,  G01N2201/0446 ,  G01N2201/062 ,  G02B21/088 ,  G02B21/36

Abstract: A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11, mounting the petri dish 11 on a waveguide 21 via water 13, emitting illumination light L1 into the waveguide 21 and emitting the illumination light L1 to the stem cells C in the petri dish 11 via the water 13, and detecting scattered light L2, the scattered light L2 being the illumination light L1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21. Then, in the light image detected by means of the scattered light L2, a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.

公开(公告)号：EP1494007B1

公开(公告)日：2014-06-04

申请号：EP04014796.9

申请日：2004-06-24

Applicant: Tecan Trading AG

Inventor： Erlbacher, Andreas ,  Niggl, Lutz ,  Krutzenbichler, Alois

IPC: G01N21/03 ,  G01N21/64 ,  G01N21/25 ,  G01N21/53 ,  G01N21/59 ,  B01L3/00

CPC classification number: G01N21/6452 ,  B01L3/5085 ,  B01L2300/0654 ,  B01L2300/0829 ,  B01L2300/168 ,  G01N21/0303 ,  G01N21/253 ,  G01N21/59 ,  G01N2021/6463 ,  G01N2021/6491 ,  G01N2021/651 ,  G01N2201/0446 ,  G01N2201/1042 ,  Y10S436/809

公开(公告)号：EP2504103A2

公开(公告)日：2012-10-03

申请号：EP10781799.1

申请日：2010-11-22

Applicant: 3M Innovative Properties Company

Inventor： HALVERSON, Kurt J. ,  KENNEY, Raymond J. ,  BENSON, Olester Jr. ,  JOHNSTON, Raymond P. ,  MAO, Guoping ,  FLEMING, Patrick R. ,  TIERS, George Van Dyke ,  JING, Naiyong

IPC: B01L3/00 ,  B29C59/00 ,  G01N21/64

CPC classification number: G01N21/75 ,  B01L3/50853 ,  B01L2200/0689 ,  B01L2200/12 ,  B01L2300/044 ,  B01L2300/0654 ,  B01L2300/0819 ,  B01L2300/0822 ,  B01L2300/0851 ,  B01L2300/0887 ,  B29C35/0888 ,  B29C59/046 ,  B29C59/08 ,  B29C59/10 ,  B29C59/14 ,  B29C59/16 ,  B29C2035/0827 ,  B29C2059/023 ,  G01N21/03 ,  G01N21/253 ,  G01N21/31 ,  G01N21/55 ,  G01N21/6428 ,  G01N21/6452 ,  G01N2021/0346 ,  G01N2201/0446 ,  G01N2201/064 ,  G01N2201/08

Abstract: The disclosure provides microstructured articles and methods useful for detecting an analyte in a sample. The articles include microwell arrays. The articles can be used with an optical system component in methods to detect or characterize an analyte.

Abstract translation: 本公开提供了用于检测样品中的分析物的微结构化制品和方法。 文章包括微孔阵列。 在用于检测或表征分析物的方法中，物品可以与光学系统组件一起使用。

公开(公告)号：EP1882189A2

公开(公告)日：2008-01-30

申请号：EP06784370.6

申请日：2006-04-20

Applicant: Fluidigm Corporation

Inventor： SUN, Gang ,  HARRIS, Greg ,  MAY, Andrew ,  SELF, Kyle ,  FARRELL, Kevin ,  WYATT, Paul

IPC: G01N35/00

CPC classification number: G01N35/00029 ,  B01L3/5027 ,  B01L3/502715 ,  B01L2200/10 ,  B01L2300/06 ,  B01L2300/0654 ,  B01L2300/0829 ,  C12Q1/686 ,  G01N15/1475 ,  G01N21/6428 ,  G01N35/00732 ,  G01N35/00871 ,  G01N35/0092 ,  G01N35/0099 ,  G01N35/1065 ,  G01N2035/00148 ,  G01N2035/00237 ,  G01N2035/00752 ,  G01N2035/0091 ,  G01N2035/1034 ,  G01N2201/025 ,  G01N2201/0446 ,  Y10S901/01 ,  Y10T436/11

Abstract: Systems for managing workflows to perform chemical or biological reactions using microfluidic devices.

公开(公告)号：EP1390720A2

公开(公告)日：2004-02-25

申请号：EP02736265.6

申请日：2002-05-02

Applicant: Technische Universiteit Delft

Inventor： IORDANOV, Ventzeslav, Petrov ,  SARRO, Pasqualina, Maria ,  WOLFFENBUTTEL, Reinoud, Felix ,  VELLEKOOP, Michael, Johannes

IPC: G01N21/25 ,  G01N21/64 ,  G01N21/03 ,  G01N33/551 ,  C12Q1/68

CPC classification number: G01N21/6452 ,  C12Q1/26 ,  C12Q1/32 ,  C12Q1/485 ,  G01N21/0303 ,  G01N21/6428 ,  G01N33/551 ,  G01N33/552 ,  G01N33/553 ,  G01N2021/6471 ,  G01N2021/6482 ,  G01N2201/0446 ,  G02B5/207 ,  G02B5/208

Abstract: The invention relates to a method of performing a fluorescence assay. According to the invention a filter is integrated with at least one component chosen from i) a body comprising a well, wherein the well in which the assay is being performed is exposed to excitation light in such a manner that the filter is positioned between the light-sensitive element and the excitation light source, and ii) a light-sensitive element, wherein the filter is applied at least at the light-sensitive side of the surface of the light-sensitive element, and any light that may be emitted is detected by means of the light-sensitive element. The invention also relates to an apparatus suitable for carrying out the method, as well as a method for its manufacture.